JP6592427B2 - タンパク質および核酸を検出するためのナノ細孔バイオセンサー - Google Patents
タンパク質および核酸を検出するためのナノ細孔バイオセンサー Download PDFInfo
- Publication number
- JP6592427B2 JP6592427B2 JP2016504425A JP2016504425A JP6592427B2 JP 6592427 B2 JP6592427 B2 JP 6592427B2 JP 2016504425 A JP2016504425 A JP 2016504425A JP 2016504425 A JP2016504425 A JP 2016504425A JP 6592427 B2 JP6592427 B2 JP 6592427B2
- Authority
- JP
- Japan
- Prior art keywords
- clya
- pore
- seq
- modified
- modified clya
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/255—Salmonella (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Food Science & Technology (AREA)
- Nanotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Electrochemistry (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361805068P | 2013-03-25 | 2013-03-25 | |
| US61/805,068 | 2013-03-25 | ||
| GB1313477.0 | 2013-07-29 | ||
| GBGB1313477.0A GB201313477D0 (en) | 2013-07-29 | 2013-07-29 | Nanopore biosensors for detection of proteins and nucleic acids |
| PCT/BE2014/000013 WO2014153625A1 (en) | 2013-03-25 | 2014-03-25 | Nanopore biosensors for detection of proteins and nucleic acids |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016516735A JP2016516735A (ja) | 2016-06-09 |
| JP2016516735A5 JP2016516735A5 (enExample) | 2017-04-27 |
| JP6592427B2 true JP6592427B2 (ja) | 2019-10-16 |
Family
ID=49167071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016504425A Active JP6592427B2 (ja) | 2013-03-25 | 2014-03-25 | タンパク質および核酸を検出するためのナノ細孔バイオセンサー |
Country Status (9)
| Country | Link |
|---|---|
| US (7) | US10006905B2 (enExample) |
| EP (1) | EP2978773B1 (enExample) |
| JP (1) | JP6592427B2 (enExample) |
| CN (1) | CN105358567B (enExample) |
| AU (1) | AU2014245783B2 (enExample) |
| BR (1) | BR112015024452B1 (enExample) |
| CA (1) | CA2904202C (enExample) |
| GB (1) | GB201313477D0 (enExample) |
| WO (1) | WO2014153625A1 (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| EP3097210B2 (en) | 2014-01-22 | 2022-04-20 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| CN119899249A (zh) | 2014-09-01 | 2025-04-29 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
| US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
| WO2016077313A1 (en) * | 2014-11-11 | 2016-05-19 | Bgi Shenzhen | Multi-pass sequencing |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| CN107735686B (zh) * | 2015-04-14 | 2021-06-11 | 鲁汶天主教大学 | 具有内部蛋白质衔接子的纳米孔 |
| CN109072295B (zh) * | 2015-12-08 | 2022-05-31 | 鲁汶大学研究与发展中心 | 修饰的纳米孔，包含其的组合物及其应用 |
| KR102222191B1 (ko) | 2016-03-02 | 2021-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 기공 |
| CN118272512A (zh) | 2016-04-06 | 2024-07-02 | 牛津纳米孔科技公开有限公司 | 突变体孔 |
| WO2017184866A1 (en) | 2016-04-21 | 2017-10-26 | F. Hoffmann-La Roche Ag | Alpha-hemolysin variants and uses thereof |
| FR3053119A1 (fr) * | 2016-06-24 | 2017-12-29 | Excilone | Procede de detection electrique de peptides, proteines et autres macromolecules |
| GB201612458D0 (en) * | 2016-07-14 | 2016-08-31 | Howorka Stefan And Pugh Genevieve | Membrane spanning DNA nanopores for molecular transport |
| WO2018039587A1 (en) * | 2016-08-26 | 2018-03-01 | Ohio State Innovation Foundation | Phi29 nanochannel for early detection of breast cancer biomarkers |
| CA3053086A1 (en) | 2017-02-10 | 2018-08-16 | Oxford Nanopore Technologies Limited | Modified nanopores, compositions comprising the same, and uses thereof |
| GB201705764D0 (en) | 2017-04-10 | 2017-05-24 | Imp Innovations Ltd | Analyte detection method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| EP3645552B1 (en) | 2017-06-30 | 2023-06-28 | Vib Vzw | Novel protein pores |
| US11567061B2 (en) | 2017-07-17 | 2023-01-31 | President And Fellows Of Harvard College | Nanopore-matched protein shuttle for molecular characterization |
| US11639922B2 (en) | 2017-07-17 | 2023-05-02 | President And Fellows Of Harvard College | Nanopore-matched protein shuttle for molecular characterization and methodology for data analysis thereof |
| CN118949941A (zh) | 2018-02-12 | 2024-11-15 | 牛津纳米孔科技公开有限公司 | 纳米孔组件及其用途 |
| GB201809207D0 (en) * | 2018-06-05 | 2018-07-25 | Univ Oxford Innovation Ltd | Molecular electronic device |
| JP2022500074A (ja) | 2018-09-11 | 2022-01-04 | レイクスユニフェルシテイト フローニンゲン | 調節可能な孔直径を有する生物学的ナノポアと分析ツールとしてのその使用 |
| US20200326325A1 (en) | 2019-04-12 | 2020-10-15 | Lisa Diamond | Nanosensor chip with compound nanopores |
| CN111912892B (zh) * | 2019-05-07 | 2022-03-25 | 南京大学 | 气单胞菌溶素纳米孔通道在生物磷酸化及相关酶分析中的应用 |
| CN112708544A (zh) * | 2019-10-25 | 2021-04-27 | 成都今是科技有限公司 | 基因测序的测量装置及其测量方法 |
| CN111235224B (zh) * | 2020-01-14 | 2023-06-20 | 广东工业大学 | 一种基于磁泳分离的生物分子精确修饰方法及装置 |
| CN112481363B (zh) * | 2020-03-09 | 2024-05-28 | 南京大学 | 突变Aerolysin单体在检测RNA碱基序列及RNA修饰方面的应用 |
| WO2021182957A1 (en) | 2020-03-11 | 2021-09-16 | Rijksuniversiteit Groningen | Engineered plyab nanopores and uses thereof. |
| CN112480204A (zh) * | 2020-04-13 | 2021-03-12 | 南京大学 | 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法 |
| WO2021236828A1 (en) * | 2020-05-20 | 2021-11-25 | The Board Of Trustees Of The University Of Illinois | Methods and compositions for rapid direct detection and differentiation of infectious from noninfectious virus |
| KR102309424B1 (ko) * | 2020-09-28 | 2021-10-07 | 한국생명공학연구원 | 나노포어를 이용한 리보스위치-표적 물질의 스크리닝 방법 |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| CN120435657A (zh) | 2022-10-28 | 2025-08-05 | 格罗宁根大学 | 蛋白质的基于纳米孔的分析 |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| WO2026052811A2 (en) | 2024-09-06 | 2026-03-12 | Oxford Nanopore Technologies Plc | Method |
| WO2026052817A1 (en) | 2024-09-06 | 2026-03-12 | Oxford Nanopore Technologies Plc | Method |
Family Cites Families (152)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI82266C (fi) | 1982-10-19 | 1991-02-11 | Cetus Corp | Foerfarande foer framstaellning av il-2 -mutein. |
| US5198543A (en) | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| US5777078A (en) | 1993-04-28 | 1998-07-07 | Worcester Foundation For Experimental Biology | Triggered pore-forming agents |
| EP0753071A1 (en) | 1993-04-28 | 1997-01-15 | Worcester Foundation For Experimental Biology | Cell-targeted lytic pore-forming agents |
| DE4320201A1 (de) | 1993-06-18 | 1995-01-12 | Asta Medica Ag | Verwendung von Cetrorelix und weiteren Nona- und Dekapeptiden zur Herstellung eines Arzneimittels zur Bekämpfung von Aids und zur Wachstumsstimulation |
| US5386373A (en) | 1993-08-05 | 1995-01-31 | Pavilion Technologies, Inc. | Virtual continuous emission monitoring system with sensor validation |
| US5561043A (en) | 1994-01-31 | 1996-10-01 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
| US7569341B2 (en) | 1994-01-31 | 2009-08-04 | Trustees Of Boston University | Nucleic acid directed immobilization arrays and methods of assembly |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| JP3891620B2 (ja) | 1996-11-14 | 2007-03-14 | 株式会社アイシン・コスモス研究所 | ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法 |
| US20020197614A1 (en) | 1997-05-16 | 2002-12-26 | Mosaic Technologies, Inc. | Electrophoretic analysis of target molecules using adapter molecules |
| WO1999005167A1 (en) | 1997-07-25 | 1999-02-04 | University Of Massachusetts | Designed protein pores as components for biosensors |
| US6087099A (en) | 1997-09-08 | 2000-07-11 | Myriad Genetics, Inc. | Method for sequencing both strands of a double stranded DNA in a single sequencing reaction |
| US6127166A (en) | 1997-11-03 | 2000-10-03 | Bayley; Hagan | Molluscan ligament polypeptides and genes encoding them |
| JPH11137260A (ja) | 1997-11-06 | 1999-05-25 | Soyaku Gijutsu Kenkyusho:Kk | 抗インフルエンザウイルス環状ダンベル型ｒｎａ−ｄｎａキメラ化合物及び抗インフルエンザウイルス剤 |
| US6123819A (en) | 1997-11-12 | 2000-09-26 | Protiveris, Inc. | Nanoelectrode arrays |
| DE19826758C1 (de) | 1998-06-15 | 1999-10-21 | Soft Gene Gmbh | Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte |
| US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
| US7155344B1 (en) | 1998-07-27 | 2006-12-26 | Caliper Life Sciences, Inc. | Distributed database for analytical instruments |
| US6150112A (en) | 1998-09-18 | 2000-11-21 | Yale University | Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US6426231B1 (en) | 1998-11-18 | 2002-07-30 | The Texas A&M University System | Analyte sensing mediated by adapter/carrier molecules |
| NO986133D0 (no) | 1998-12-23 | 1998-12-23 | Preben Lexow | FremgangsmÕte for DNA-sekvensering |
| EP1192103A1 (en) | 1999-06-22 | 2002-04-03 | President And Fellows of Harvard College | Control of solid state dimensional features |
| CA2376650C (en) | 1999-06-29 | 2008-01-15 | University Health Network | Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes |
| CA2381139A1 (en) | 1999-08-31 | 2001-03-08 | Michael Niederweis | Method for the production of a channel forming protein |
| EP1238105A2 (en) | 1999-12-02 | 2002-09-11 | Molecular Staging Inc. | Generation of single-strand circular dna from linear self-annealing segments |
| US6916665B2 (en) * | 2000-02-11 | 2005-07-12 | The Texas A&M University System | Biosensor compositions and methods of use |
| US7078501B2 (en) | 2000-02-25 | 2006-07-18 | Invitrogen Corporation | Topoisomerase linker-mediated amplification methods |
| AU2001250912B2 (en) | 2000-03-21 | 2007-01-04 | Curagen Corporation | VEGF-modulated genes and methods employing them |
| JP2003527849A (ja) | 2000-03-22 | 2003-09-24 | キュラゲン コーポレイション | 新規ペプチド及びこれをコードする核酸 |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US7839450B2 (en) | 2000-09-25 | 2010-11-23 | Sensovation Ag | Image sensor device, apparatus and method for optical measurements |
| AU2002239284A1 (en) | 2000-11-27 | 2002-06-03 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US20030087232A1 (en) | 2001-01-25 | 2003-05-08 | Fred Christians | Methods for screening polypeptides |
| US7807408B2 (en) | 2001-03-19 | 2010-10-05 | President & Fellows Of Harvard College | Directed evolution of proteins |
| JP2002355035A (ja) | 2001-05-08 | 2002-12-10 | Inst Of Physical & Chemical Res | スフィンゴミエリン検出プローブ |
| US6995236B2 (en) | 2001-05-08 | 2006-02-07 | Riken | Sphingomyelin detecting probe |
| US6863833B1 (en) | 2001-06-29 | 2005-03-08 | The Board Of Trustees Of The Leland Stanford Junior University | Microfabricated apertures for supporting bilayer lipid membranes |
| WO2003004992A2 (en) | 2001-07-03 | 2003-01-16 | The Regents Of The University Of California | Mammalian sweet and amino acid heterodimeric taste receptors |
| CA2450435C (en) | 2001-08-31 | 2009-02-03 | Gentex Corporation | Vehicle lamp assembly with heat sink |
| DE60324778D1 (de) | 2002-03-15 | 2009-01-02 | Nuevolution As | Eine verbesserte methode zur synthese von matritzenabhängigen molekülen |
| WO2003095669A1 (en) | 2002-05-10 | 2003-11-20 | The Texas A & M University System | Stochastic sensing through covalent interactions |
| US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| AU2004225520A1 (en) | 2003-03-25 | 2004-10-14 | Stratagene | DNA polymerase fusions and uses thereof |
| US7163658B2 (en) | 2003-04-23 | 2007-01-16 | Rouvain Bension | Rapid sequencing of polymers |
| US7344882B2 (en) | 2003-05-12 | 2008-03-18 | Bristol-Myers Squibb Company | Polynucleotides encoding variants of the TRP channel family member, LTRPC3 |
| AU2003904237A0 (en) | 2003-08-08 | 2003-08-21 | Garvan Institute Of Medical Research | Novel translocation assay |
| WO2005056750A2 (en) | 2003-12-11 | 2005-06-23 | Quark Biotech, Inc. | Inversion-duplication of nucleic acids and libraries prepared thereby |
| US7424370B2 (en) | 2004-02-06 | 2008-09-09 | Council Of Scientific And Industrial Research | Computational method for identifying adhesin and adhesin-like proteins of therapeutic potential |
| JP2005253427A (ja) | 2004-03-15 | 2005-09-22 | Aisin Seiki Co Ltd | 核酸検出方法及び核酸単離方法 |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| WO2006020775A2 (en) | 2004-08-13 | 2006-02-23 | Harvard University | An ultra high-throughput opti-nanopore dna readout platform |
| US20060105461A1 (en) | 2004-10-22 | 2006-05-18 | May Tom-Moy | Nanopore analysis system |
| US7867716B2 (en) | 2004-12-21 | 2011-01-11 | The Texas A&M University System | High temperature ion channels and pores |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| US7272518B2 (en) | 2005-07-01 | 2007-09-18 | Square D Company | Automated hierarchy classification in utility monitoring systems |
| WO2007024997A2 (en) | 2005-08-22 | 2007-03-01 | Fermalogic, Inc. | Methods of increasing production of secondary metabolites |
| US7877154B2 (en) | 2005-09-30 | 2011-01-25 | Fisher-Rosemount Systems, Inc. | Method and system for controlling a batch process |
| KR100730350B1 (ko) | 2005-10-17 | 2007-06-19 | 삼성전자주식회사 | 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치 |
| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| AU2006331512B2 (en) | 2005-12-22 | 2012-02-23 | Pacific Biosciences Of California, Inc. | Active surface coupled polymerases |
| US7932029B1 (en) | 2006-01-04 | 2011-04-26 | Si Lok | Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities |
| US7849581B2 (en) | 2006-05-05 | 2010-12-14 | University Of Utah Research Foundation | Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof |
| CN101578373A (zh) | 2006-09-06 | 2009-11-11 | 费斯生物制药公司 | 融合肽治疗组合物 |
| US7638034B2 (en) | 2006-09-21 | 2009-12-29 | Los Alamos National Security, Llc | Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions |
| US20100311602A1 (en) | 2006-10-13 | 2010-12-09 | J. Craig Venter Institute, Inc. | Sequencing method |
| US8594848B2 (en) | 2006-11-28 | 2013-11-26 | Lester F. Ludwig | Reconfigurable chemical process systems |
| EP2122344B8 (en) | 2007-02-20 | 2019-08-21 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor system |
| WO2008124107A1 (en) | 2007-04-04 | 2008-10-16 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| WO2008147816A2 (en) * | 2007-05-22 | 2008-12-04 | Cornell Research Foundation, Inc. | Compositions and methods for the display of proteins on the surface of bacteria and their derived vesicles and uses thereof |
| US8158344B2 (en) | 2007-06-29 | 2012-04-17 | Duke University | Methods and compositions for correlating genetic markers with multiple sclerosis |
| GB0716005D0 (en) | 2007-08-16 | 2007-09-26 | Imp Innovations Ltd | Single molecule spectroscopy using nanoporpus membranes |
| GB0716264D0 (en) | 2007-08-21 | 2007-09-26 | Isis Innovation | Bilayers |
| EP3540436B1 (en) | 2007-09-12 | 2023-11-01 | President And Fellows Of Harvard College | High-resolution molecular sensor |
| GB2453377A (en) | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
| WO2009052214A2 (en) | 2007-10-15 | 2009-04-23 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8502972B2 (en) | 2007-12-31 | 2013-08-06 | Fujirebio Inc. | Clusters of microresonators for cavity mode optical sensing |
| US8231969B2 (en) | 2008-03-26 | 2012-07-31 | University Of Utah Research Foundation | Asymmetrically functionalized nanoparticles |
| JP2011515102A (ja) | 2008-03-28 | 2011-05-19 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | 核酸シーケンシング用組成物及び方法 |
| US8772041B2 (en) | 2008-05-22 | 2014-07-08 | The Regents Of The University Of California | Membrane precursors and membranes formed therefrom |
| US8652771B2 (en) | 2008-05-28 | 2014-02-18 | University of Souther California | Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects |
| US20110229877A1 (en) | 2008-07-07 | 2011-09-22 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| CA2730068A1 (en) | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| DE202009019021U1 (de) | 2008-09-22 | 2015-09-24 | University Of Washington | Msp-nanoporen |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| GB0820927D0 (en) | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| EP2359289A4 (en) | 2008-11-26 | 2015-11-11 | Illumina Inc | METHODS AND SYSTEMS FOR ANALYZING SEQUENCING DATA |
| EP2391732B1 (en) | 2009-01-30 | 2015-05-27 | Oxford Nanopore Technologies Limited | Methods using adaptors for nucleic acid constructs in transmembrane sequencing |
| CN102482330A (zh) | 2009-01-30 | 2012-05-30 | 牛津纳米孔技术有限公司 | 酶突变体 |
| US8399219B2 (en) | 2009-02-23 | 2013-03-19 | Cytomx Therapeutics, Inc. | Protease activatable interferon alpha proprotein |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| FR2943656A1 (fr) | 2009-03-25 | 2010-10-01 | Air Liquide | Procede et installation de production d'hydrogene mettant en oeuvre un compresseur thermocinetique |
| CA2758617C (en) | 2009-04-20 | 2017-10-03 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor array |
| EP2507387B1 (en) | 2009-12-01 | 2017-01-25 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument and method |
| FR2955773B1 (fr) | 2010-02-01 | 2017-05-26 | Commissariat Energie Atomique | Complexe moleculaire de ciblage des antigenes vers les cellules presentatrices d'antigene et ses applications pour la vaccination |
| ES2978883T3 (es) | 2010-02-23 | 2024-09-23 | Univ Washington | Secuenciación de analitos con nanoporos |
| EP2556085A2 (en) | 2010-04-05 | 2013-02-13 | Bar-Ilan University | Protease-activatable pore-forming polypeptides |
| WO2012042226A2 (en) | 2010-10-01 | 2012-04-05 | Oxford Nanopore Technologies Limited | Biochemical analysis apparatus and rotary valve |
| CN102116783B (zh) | 2010-12-31 | 2013-05-29 | 北京普源精电科技有限公司 | 一种波形显示方法 |
| GB201100516D0 (en) | 2011-01-12 | 2011-02-23 | Isis Innovation | Method using fluorinated amphiphiles |
| CN102174554A (zh) | 2011-01-24 | 2011-09-07 | 内蒙古民族大学 | 双控双调节原核表达载体系统及其构建方法和用途 |
| BR112013020411B1 (pt) | 2011-02-11 | 2021-09-08 | Oxford Nanopore Technologies Limited | Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo |
| SG10201604316WA (en) | 2011-05-27 | 2016-07-28 | Oxford Nanopore Tech Ltd | Coupling method |
| US9580480B2 (en) | 2011-05-31 | 2017-02-28 | Massachusetts Institute Of Technology | Cell-directed synthesis of multifunctional nanopatterns and nanomaterials |
| KR20140050067A (ko) | 2011-07-25 | 2014-04-28 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법 |
| EP3663412B1 (en) | 2011-09-23 | 2022-03-09 | Oxford Nanopore Technologies PLC | Analysis of a polymer comprising polymer units by means of translocation through a nanopore |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| CA2861808C (en) | 2011-12-29 | 2021-02-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| EP2798083B1 (en) | 2011-12-29 | 2017-08-09 | Oxford Nanopore Technologies Limited | Method for characterising a polynucelotide by using a xpd helicase |
| CA2861457A1 (en) | 2012-01-20 | 2013-07-25 | Genia Technologies, Inc. | Nanopore based molecular detection and sequencing |
| CA2864035C (en) * | 2012-02-15 | 2021-05-18 | Oxford Nanopore Technologies Limited | A method for determining the presence of an analyte using an aptamer |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| TWI655213B (zh) | 2012-07-13 | 2019-04-01 | Menicon Co., Ltd. | 自我組織化肽衍生物的製造方法 |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| AU2013291763B2 (en) | 2012-07-19 | 2019-06-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| CA2879355C (en) | 2012-07-19 | 2021-09-21 | Oxford Nanopore Technologies Limited | Helicase construct and its use in characterising polynucleotides |
| JP6375301B2 (ja) | 2012-10-26 | 2018-08-15 | オックスフォード ナノポール テクノロジーズ リミテッド | 液滴界面 |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| US20140206842A1 (en) | 2013-01-22 | 2014-07-24 | Muhammed Majeed | Peptides Modified with Triterpenoids and Small Organic Molecules: Synthesis and use in Cosmeceutical |
| WO2014122654A2 (en) | 2013-02-07 | 2014-08-14 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Hybrid nanopores and uses thereof for detection of analytes |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| EP2970951B1 (en) | 2013-03-13 | 2019-02-20 | Illumina, Inc. | Methods for nucleic acid sequencing |
| US10613076B2 (en) | 2013-03-14 | 2020-04-07 | The Trustees Of Boston University | Optoelectronic control of solid-state nanopores |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| EP3004378B1 (en) | 2013-05-24 | 2017-12-20 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing using nanopores |
| WO2015051378A1 (en) | 2013-10-04 | 2015-04-09 | University Of Washington Through Its Center For Commercialization | Systems and methods for nanopore-based analysis of nucleic acids |
| EP3575410A3 (en) | 2013-10-18 | 2020-03-04 | Oxford Nanopore Technologies Limited | Modified enzymes |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| ES2735015T3 (es) | 2013-11-26 | 2019-12-13 | Illumina Inc | Composiciones y métodos para secuenciar polinucleótidos |
| WO2015097289A1 (en) | 2013-12-24 | 2015-07-02 | Vib Vzw | Secretion and functional display of chimeric polypeptides |
| EP3097210B2 (en) | 2014-01-22 | 2022-04-20 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| US10337060B2 (en) | 2014-04-04 | 2019-07-02 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| FR3023394B1 (fr) | 2014-07-02 | 2017-12-29 | Adn Access Data Networks | Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture |
| CN119899249A (zh) | 2014-09-01 | 2025-04-29 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
| US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| CN107735686B (zh) | 2015-04-14 | 2021-06-11 | 鲁汶天主教大学 | 具有内部蛋白质衔接子的纳米孔 |
| CN109072295B (zh) | 2015-12-08 | 2022-05-31 | 鲁汶大学研究与发展中心 | 修饰的纳米孔，包含其的组合物及其应用 |
| KR102222191B1 (ko) | 2016-03-02 | 2021-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 기공 |
| CN118272512A (zh) | 2016-04-06 | 2024-07-02 | 牛津纳米孔科技公开有限公司 | 突变体孔 |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| EP3645552B1 (en) | 2017-06-30 | 2023-06-28 | Vib Vzw | Novel protein pores |
-
2013
- 2013-07-29 GB GBGB1313477.0A patent/GB201313477D0/en not_active Ceased
-
2014
- 2014-03-25 JP JP2016504425A patent/JP6592427B2/ja active Active
- 2014-03-25 US US14/779,895 patent/US10006905B2/en active Active
- 2014-03-25 AU AU2014245783A patent/AU2014245783B2/en active Active
- 2014-03-25 BR BR112015024452-1A patent/BR112015024452B1/pt active IP Right Grant
- 2014-03-25 EP EP14724622.7A patent/EP2978773B1/en active Active
- 2014-03-25 WO PCT/BE2014/000013 patent/WO2014153625A1/en not_active Ceased
- 2014-03-25 CA CA2904202A patent/CA2904202C/en active Active
- 2014-03-25 CN CN201480018571.6A patent/CN105358567B/zh active Active
-
2016
- 2016-06-24 US US15/192,317 patent/US20160370358A1/en not_active Abandoned
-
2018
- 2018-05-21 US US15/984,974 patent/US10514378B2/en active Active
-
2019
- 2019-07-24 US US16/521,475 patent/US10802015B2/en active Active
- 2019-09-11 US US16/568,225 patent/US10976311B2/en active Active
-
2021
- 2021-03-09 US US17/196,810 patent/US11761956B2/en active Active
-
2023
- 2023-08-02 US US18/363,859 patent/US20240345073A9/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN105358567A (zh) | 2016-02-24 |
| US20210405039A1 (en) | 2021-12-30 |
| US20160053300A1 (en) | 2016-02-25 |
| US20160370358A1 (en) | 2016-12-22 |
| US20200072824A1 (en) | 2020-03-05 |
| CN105358567B (zh) | 2021-12-10 |
| WO2014153625A1 (en) | 2014-10-02 |
| BR112015024452B1 (pt) | 2023-12-05 |
| EP2978773B1 (en) | 2020-05-27 |
| US20180335425A1 (en) | 2018-11-22 |
| US10006905B2 (en) | 2018-06-26 |
| EP2978773A1 (en) | 2016-02-03 |
| US10802015B2 (en) | 2020-10-13 |
| GB201313477D0 (en) | 2013-09-11 |
| CA2904202A1 (en) | 2014-10-02 |
| BR112015024452A2 (pt) | 2017-10-24 |
| US11761956B2 (en) | 2023-09-19 |
| US20240044881A1 (en) | 2024-02-08 |
| AU2014245783A1 (en) | 2015-10-01 |
| US10976311B2 (en) | 2021-04-13 |
| US10514378B2 (en) | 2019-12-24 |
| AU2014245783B2 (en) | 2018-08-16 |
| CA2904202C (en) | 2021-03-30 |
| US20190346431A1 (en) | 2019-11-14 |
| US20240345073A9 (en) | 2024-10-17 |
| JP2016516735A (ja) | 2016-06-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11761956B2 (en) | Nanopore biosensors for detection of proteins and nucleic acids | |
| US20220091096A1 (en) | Nanopores with internal protein adaptors | |
| CN102216783B (zh) | Msp纳米微孔和相关方法 | |
| JP2010539966A (ja) | 分子アダプター | |
| EP3485029B1 (en) | Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin | |
| JP2025060611A (ja) | 調節可能な孔直径を有する生物学的ナノポアと分析ツールとしてのその使用 | |
| JP2019507583A (ja) | 修飾ナノポア、それを含む組成物およびその使用 | |
| Class et al. | Patent application title: NANOPORE BIOSENSORS FOR DETECTION OF PROTEINS AND NUCLEIC ACIDS | |
| Huang | Engineering biological nanopores for proteomics study | |
| HK40052928A (en) | Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin | |
| Franceschini et al. | DNA analysis with the biological nanopore ClyA |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20151125 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170324 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20170324 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20180220 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20180515 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20180720 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180820 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20181211 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20190304 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190424 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20190827 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20190920 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6592427 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |