JP6587702B2 - 無細胞軟骨グラフトの調製及びその使用 - Google Patents
無細胞軟骨グラフトの調製及びその使用 Download PDFInfo
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3612—Cartilage, synovial fluid
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/362—Skin, e.g. dermal papillae
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
- A61L27/3645—Connective tissue
- A61L27/3654—Cartilage, e.g. meniscus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
Description
ステップ(1)において、動物の軟骨をダイシングして軟骨基質を作製する。
ステップ(2)において、ステップ(1)の軟骨基質をアルカリ処理する。
ステップ(3)において、ステップ(2)のアルカリ処理された軟骨基質を消毒処理する。
ステップ(4)において、ステップ(3)の消毒された軟骨基質を脱細胞化処理して無細胞軟骨グラフトを作製する。
ステップ(1)において、動物の軟骨をダイシングして複数の軟骨基質を作製する。
ステップ(2)において、ステップ(1)の軟骨基質をアルカリ処理する。
ステップ(3)において、ステップ(2)のアルカリ処理された軟骨基質を消毒処理する。
ステップ(4)において、ステップ(3)の消毒された軟骨基質を脱細胞化処理して無細胞軟骨グラフトを作製する。
実施例
材料及び方法
細胞培養
Claims (6)
- 以下のステップ(1)〜(4)を含む無細胞軟骨グラフトの調製方法であって、
ステップ(1)において、動物の軟骨をダイシングして、複数の軟骨基質を作製し、
ステップ(2)において、水酸化ナトリウム溶液でステップ(1)の軟骨基質を室温で約3−24時間処理し、
ステップ(3)において、消毒剤でステップ(2)のアルカリ処理された軟骨基質を消毒し、前記消毒剤は、アルコール、酸化剤、放射線、フェノール化合物、及び第四級アンモニウム化合物からなる群より選択され、
ステップ(4)において、ステップ(3)の消毒された軟骨基質を超臨界流体(SCF)処理して、前記無細胞軟骨グラフトを作製し、前記SCF処理は、約150−350barの圧力、30−50℃の温度で約100分間行われ、
前記SCFは、超臨界二酸化炭素(scCO 2 )であり、
前記製造方法では、前記無細胞軟骨グラフトを調製する際に、高張性塩溶液、酵素消化、又は脱水を必要としない、無細胞軟骨グラフトの調製方法。 - 前記軟骨は、ブタから得られた鼻軟骨、肋軟骨、耳軟骨、又は膝軟骨である請求項1に記載の方法。
- 各軟骨グラフトは、円盤状、ストリップ状、円錐状、又は粒子状である請求項2に記載の方法。
- 前記アルコールは、エタノール又はイソプロパノールであり、
前記酸化剤は、過酸化水素であり、
前記フェノール化合物は、パラベン、安息香酸、又はサリチル酸であり、
前記第四級アンモニウム化合物は、塩化ベンザルコニウム、塩化アルキルジメチルベンジルアンモニウム、ジデシルジメチルアンモニウムクロライド、塩化アンモニウムである請求項1に記載の方法。 - 前記温度は約37℃であり、前記圧力は約350barである請求項1に記載の方法。
- 前記無細胞軟骨グラフトの各々は、天然軟骨よりも少なくとも2倍の応力に耐えることができる請求項5に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US201562203904P | 2015-08-11 | 2015-08-11 | |
US62/203,904 | 2015-08-11 | ||
PCT/CN2016/094633 WO2017025053A1 (en) | 2015-08-11 | 2016-08-11 | Preparation of acellular cartilage graft and uses thereof |
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JP2018527047A JP2018527047A (ja) | 2018-09-20 |
JP6587702B2 true JP6587702B2 (ja) | 2019-10-09 |
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JP2017567442A Active JP6889118B2 (ja) | 2015-08-11 | 2016-08-11 | 高純度コラーゲン粒子の製造及びその使用 |
JP2017567443A Active JP6587702B2 (ja) | 2015-08-11 | 2016-08-11 | 無細胞軟骨グラフトの調製及びその使用 |
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US (2) | US10881761B2 (ja) |
EP (2) | EP3328455B1 (ja) |
JP (2) | JP6889118B2 (ja) |
KR (2) | KR102126140B1 (ja) |
CN (2) | CN108025111B (ja) |
ES (1) | ES2891828T3 (ja) |
HK (2) | HK1246358A1 (ja) |
TW (2) | TWI600660B (ja) |
WO (2) | WO2017025054A1 (ja) |
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KR101954905B1 (ko) * | 2016-08-08 | 2019-03-08 | 주식회사 도프 | 지방흡입 유출물로부터 초임계공정을 이용하여 콜라겐을 분리하는 방법 |
JP7012950B2 (ja) * | 2016-11-17 | 2022-01-31 | 地方独立行政法人東京都立産業技術研究センター | 生体組織孔閉鎖用、潰瘍保護用及び血管塞栓療術用ゾル |
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EP3334835A1 (en) | 2018-06-20 |
JP2018527047A (ja) | 2018-09-20 |
CN107849596B (zh) | 2022-02-25 |
CN107849596A (zh) | 2018-03-27 |
HK1246358A1 (zh) | 2018-09-07 |
TW201710493A (zh) | 2017-03-16 |
TW201712026A (zh) | 2017-04-01 |
EP3328455B1 (en) | 2021-07-07 |
JP6889118B2 (ja) | 2021-06-18 |
JP2018523467A (ja) | 2018-08-23 |
TWI641683B (zh) | 2018-11-21 |
EP3328455A4 (en) | 2019-04-03 |
CN108025111A (zh) | 2018-05-11 |
US20200040030A1 (en) | 2020-02-06 |
KR20180021853A (ko) | 2018-03-05 |
US20180369449A1 (en) | 2018-12-27 |
ES2891828T3 (es) | 2022-01-31 |
HK1248149A1 (zh) | 2018-10-12 |
KR102126140B1 (ko) | 2020-06-24 |
US11185610B2 (en) | 2021-11-30 |
WO2017025053A1 (en) | 2017-02-16 |
EP3334835A4 (en) | 2019-05-01 |
KR20180021851A (ko) | 2018-03-05 |
TWI600660B (zh) | 2017-10-01 |
WO2017025054A1 (en) | 2017-02-16 |
CN108025111B (zh) | 2021-04-16 |
EP3328455A1 (en) | 2018-06-06 |
KR102116620B1 (ko) | 2020-05-29 |
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