JP6512825B2 - 組換え微生物およびその使用方法 - Google Patents

組換え微生物およびその使用方法 Download PDF

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JP6512825B2
JP6512825B2 JP2014555522A JP2014555522A JP6512825B2 JP 6512825 B2 JP6512825 B2 JP 6512825B2 JP 2014555522 A JP2014555522 A JP 2014555522A JP 2014555522 A JP2014555522 A JP 2014555522A JP 6512825 B2 JP6512825 B2 JP 6512825B2
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seq
microorganism
gene
acetolactate
butanediol
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JP2015505471A (ja
JP2015505471A5 (cg-RX-API-DMAC10.html
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コエプケ,マイケル
ナガラジュ,シルパ
チェン,ウェンディ
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ランザテク・ニュージーランド・リミテッド
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01004—R,R-butanediol dehydrogenase (1.1.1.4)
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JP2014555522A 2012-01-31 2013-01-31 組換え微生物およびその使用方法 Active JP6512825B2 (ja)

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EP (1) EP2710117B1 (cg-RX-API-DMAC10.html)
JP (2) JP6512825B2 (cg-RX-API-DMAC10.html)
KR (1) KR101511639B1 (cg-RX-API-DMAC10.html)
CN (1) CN104395455B (cg-RX-API-DMAC10.html)
AU (1) AU2013215706B2 (cg-RX-API-DMAC10.html)
BR (1) BR112014018844B1 (cg-RX-API-DMAC10.html)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019088292A (ja) * 2012-01-31 2019-06-13 ランザテク・ニュージーランド・リミテッド 組換え微生物およびその使用方法

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3047028T5 (da) * 2013-09-22 2024-10-14 Lanzatech Nz Inc Fermenteringsfremgangsmåde
US20150152445A1 (en) * 2013-12-03 2015-06-04 Lanzatech New Zealand Limited Microorganisms and methods for the production of ketones
WO2015116874A1 (en) * 2014-01-30 2015-08-06 Lanzatech New Zealand Limited Recombinant microorganisms and methods of use thereof
KR102308556B1 (ko) * 2014-08-11 2021-10-07 란자테크 뉴질랜드 리미티드 변경된 일산화탄소 탈수소효소(codh) 활성을 가지는 유전자 조작된 박테리아
AU2015360786B2 (en) * 2014-12-08 2021-10-21 Lanzatech Nz, Inc. Recombinant microorganisms exhibiting increased flux through a fermentation pathway
WO2017037897A1 (ja) * 2015-09-02 2017-03-09 積水化学工業株式会社 組換え細胞、組換え細胞の製造方法、並びに、1,4-ブタンジオールの生産方法
JP6905518B2 (ja) 2015-10-13 2021-07-21 ランザテク・ニュージーランド・リミテッド エネルギー発生発酵経路を含む遺伝子操作細菌
CN108603178A (zh) * 2016-01-06 2018-09-28 株式会社西化学 Co水合酶以及使用其产生甲酸的方法
US11634733B2 (en) 2017-06-30 2023-04-25 Inv Nylon Chemicals Americas, Llc Methods, materials, synthetic hosts and reagents for the biosynthesis of hydrocarbons and derivatives thereof
US11162115B2 (en) 2017-06-30 2021-11-02 Inv Nylon Chemicals Americas, Llc Methods, synthetic hosts and reagents for the biosynthesis of hydrocarbons
US11505809B2 (en) * 2017-09-28 2022-11-22 Inv Nylon Chemicals Americas Llc Organisms and biosynthetic processes for hydrocarbon synthesis
US20190233816A1 (en) 2018-01-26 2019-08-01 Massachusetts Institute Of Technology Structure-guided chemical modification of guide rna and its applications
CN113490748A (zh) * 2019-02-25 2021-10-08 西北大学 来源于梭菌属提取物的无细胞蛋白质合成平台
CN111089914A (zh) * 2019-11-07 2020-05-01 河北首朗新能源科技有限公司 微生物发酵生产乙醇中代谢物的高效液相检测方法
CN111154683B (zh) * 2020-01-19 2022-05-13 南京工业大学 一种食甲基丁酸杆菌的优化培养方法及其应用
CN111220762A (zh) * 2020-03-24 2020-06-02 河北首朗新能源科技有限公司 一种乙醇梭菌蛋白中有机物的含量的检测方法及应用
CN114144513A (zh) * 2020-04-29 2022-03-04 朗泽科技有限公司 从气态底物发酵生产β-酮己二酸盐
JP2023520257A (ja) * 2020-06-06 2023-05-16 ランザテク,インコーポレイテッド アセトラクテートデカルボキシラーゼ遺伝子座位でのノックインを有する微生物
TW202212566A (zh) * 2020-09-25 2022-04-01 美商朗澤科技有限公司 重組微生物及其用途
CN113234653B (zh) * 2021-04-22 2022-10-04 中国科学院青岛生物能源与过程研究所 一种提高合成气发酵产乙醇含量的突变株和利用突变株的应用
US20240117393A1 (en) 2022-09-30 2024-04-11 Ajinomoto Co., Inc. Method for producing l-amino acid
CN115960808A (zh) * 2022-11-24 2023-04-14 苏州聚维元创生物科技有限公司 一种敲除基因的重组大肠杆菌及其构建方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5437631B2 (ja) * 2005-07-01 2014-03-12 ザ ユニバーシティー オブ フロリダ リサーチ ファンデーション インク エタノール産生のための組み換えホスト細胞及び培地
WO2008013996A2 (en) 2006-07-27 2008-01-31 Gevo Inc. Engineered microorganisms for increasing product yield in biotransformations, related methods and systems
DE102007048277A1 (de) * 2007-10-08 2009-04-09 Agraferm Technologies Ag Verfahren und Vorrichtung zur mikrobiellen Herstellung eines bestimmten Produktes und Methan
KR101375029B1 (ko) * 2007-11-13 2014-03-14 란자테크 뉴질랜드 리미티드 신규 세균 및 이의 이용 방법
US8119378B2 (en) 2008-03-12 2012-02-21 Lanzatech New Zealand Limited Microbial alcohol production process
AU2009258344B2 (en) * 2008-06-09 2013-11-07 Lanzatech Nz, Inc. Production of butanediol by anaerobic microbial fermentation
DE102009002583A1 (de) 2009-04-23 2010-10-28 Evonik Degussa Gmbh Zellen und Verfahren zur Herstellung von Aceton
US8143037B2 (en) * 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii
BR112013003644B1 (pt) * 2010-07-28 2020-11-17 Lanzatech New Zealand Limited isolado biologicamente puro de uma bactéria clostridium autoethanogenum
EP2710117B1 (en) * 2012-01-31 2016-04-27 Lanzatech New Zealand Limited Recombinant microorganisms and methods of use thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019088292A (ja) * 2012-01-31 2019-06-13 ランザテク・ニュージーランド・リミテッド 組換え微生物およびその使用方法

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KR20130138866A (ko) 2013-12-19
CN104395455B (zh) 2020-05-19
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EP2710117A2 (en) 2014-03-26
EP2710117B1 (en) 2016-04-27
BR112014018844B1 (pt) 2022-09-27
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JP2019088292A (ja) 2019-06-13
JP6898365B2 (ja) 2021-07-07
CN104395455A (zh) 2015-03-04
CA2862790A1 (en) 2013-08-08
BR112014018844A2 (pt) 2020-08-18
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