JP6499081B2 - アスペルギルス・フミガタス(Aspergillusfumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichodermareesei)宿主細胞、及びその使用方法 - Google Patents

アスペルギルス・フミガタス(Aspergillusfumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichodermareesei)宿主細胞、及びその使用方法 Download PDF

Info

Publication number
JP6499081B2
JP6499081B2 JP2015547962A JP2015547962A JP6499081B2 JP 6499081 B2 JP6499081 B2 JP 6499081B2 JP 2015547962 A JP2015547962 A JP 2015547962A JP 2015547962 A JP2015547962 A JP 2015547962A JP 6499081 B2 JP6499081 B2 JP 6499081B2
Authority
JP
Japan
Prior art keywords
variant
afgatr
starch
composition
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015547962A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016500267A5 (enExample
JP2016500267A (ja
Inventor
ジー、ジン
ファー、リン
リー、スン・ホー
リ、ジャルセン
シェティ、ジャヤラマ・ケー
タン、ゾンメイ
ジャン、ボー
ゾン、クン
Original Assignee
ダニスコ・ユーエス・インク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ダニスコ・ユーエス・インク filed Critical ダニスコ・ユーエス・インク
Publication of JP2016500267A publication Critical patent/JP2016500267A/ja
Publication of JP2016500267A5 publication Critical patent/JP2016500267A5/ja
Application granted granted Critical
Publication of JP6499081B2 publication Critical patent/JP6499081B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
JP2015547962A 2012-12-11 2013-11-21 アスペルギルス・フミガタス(Aspergillusfumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichodermareesei)宿主細胞、及びその使用方法 Active JP6499081B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2012086349 2012-12-11
CNPCT/CN2012/086349 2012-12-11
PCT/US2013/071154 WO2014092960A1 (en) 2012-12-11 2013-11-21 Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019046834A Division JP2019115353A (ja) 2012-12-11 2019-03-14 アスペルギルス・フミガタス(Aspergillus・fumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichoderma・reesei)宿主細胞、及びその使用方法

Publications (3)

Publication Number Publication Date
JP2016500267A JP2016500267A (ja) 2016-01-12
JP2016500267A5 JP2016500267A5 (enExample) 2016-12-28
JP6499081B2 true JP6499081B2 (ja) 2019-04-10

Family

ID=49876974

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2015547962A Active JP6499081B2 (ja) 2012-12-11 2013-11-21 アスペルギルス・フミガタス(Aspergillusfumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichodermareesei)宿主細胞、及びその使用方法
JP2019046834A Pending JP2019115353A (ja) 2012-12-11 2019-03-14 アスペルギルス・フミガタス(Aspergillus・fumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichoderma・reesei)宿主細胞、及びその使用方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2019046834A Pending JP2019115353A (ja) 2012-12-11 2019-03-14 アスペルギルス・フミガタス(Aspergillus・fumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichoderma・reesei)宿主細胞、及びその使用方法

Country Status (5)

Country Link
US (3) US20160115509A1 (enExample)
EP (2) EP2931872B1 (enExample)
JP (2) JP6499081B2 (enExample)
CA (1) CA2893270C (enExample)
WO (1) WO2014092960A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2935920T3 (es) 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación
CA2869047C (en) 2012-03-30 2022-11-01 Novozymes North America, Inc. Processes of producing fermentation products
EP3227430B1 (en) * 2014-12-01 2021-05-19 Danisco US Inc. Fungal host strains, dna constructs, and methods of use
US10612058B2 (en) * 2014-12-19 2020-04-07 Danisco Us Inc Methods for saccharifying a starch substrate
US20190010473A1 (en) * 2015-12-21 2019-01-10 Danisco Us Inc. Improved granular starch conversion enzymes and methods
CN109072267A (zh) * 2015-12-21 2018-12-21 丹尼斯科美国公司 改善的颗粒状淀粉转化酶和方法
BR112018012617A2 (pt) * 2015-12-21 2018-12-04 C Koops Bart enzimas e métodos de conversão de amido granular aprimorada
MX2019003250A (es) * 2016-09-23 2019-08-29 Dupont Nutrition Biosci Aps Uso de alfa-1,4/1,6-glucosido hidrolasas activas a ph bajo como aditivo alimentario para rumiantes para mejorar la digestion de almidon.
EP3592849A1 (en) 2017-03-07 2020-01-15 Danisco US Inc. Thermostable glucoamylase and methods of use, thereof
WO2019047199A1 (en) 2017-09-11 2019-03-14 Danisco Us Inc. GLUCOAMYLASE AND METHODS OF USE THEREOF
EP3673039B1 (en) * 2017-09-29 2025-03-19 International N&H Denmark ApS Production of brewer's wort having increased fermentable sugars
CN114107360B (zh) * 2022-01-28 2022-04-08 中国农业科学院北京畜牧兽医研究所 一种通过干扰磷酸酶基因提高里氏木霉纤维素酶表达的方法

Family Cites Families (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
JPS57174089A (en) 1981-04-20 1982-10-26 Novo Industri As Chain dividing enzyme product
US5422267A (en) 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
EP0208491B1 (en) 1985-07-03 1993-08-25 Genencor International, Inc. Hybrid polypeptides and process for their preparation
EP0218272B1 (en) 1985-08-09 1992-03-18 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
EP0258068B1 (en) 1986-08-29 1994-08-31 Novo Nordisk A/S Enzymatic detergent additive
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
DE3854249T2 (de) 1987-08-28 1996-02-29 Novonordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
EP0394352B1 (en) 1988-01-07 1992-03-11 Novo Nordisk A/S Enzymatic detergent
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
JP2728531B2 (ja) 1988-03-24 1998-03-18 ノボ ノルディスク アクティーゼルスカブ セルラーゼ調製品
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
US5055403A (en) 1989-06-26 1991-10-08 Enzyme Bio-Systems, Ltd. Thermoduric and aciduric pullulanase enzyme and method for its production
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
KR100236540B1 (ko) 1990-04-14 2000-01-15 레클로우크스 라우에르 알카리성 바실러스-리파제, 이를 코-딩하는 dna 서열 및 리파제를 생산하는 바실러스 균주
ATE118545T1 (de) 1990-05-09 1995-03-15 Novo Nordisk As Eine ein endoglucanase enzym enthaltende zellulasezubereitung.
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
DK0548228T3 (da) 1990-09-13 1999-05-10 Novo Nordisk As Lipasevarianter
WO1992006154A1 (en) 1990-09-28 1992-04-16 The Procter & Gamble Company Polyhydroxy fatty acid amide surfactants to enhance enzyme performance
CA2097180C (en) 1990-12-10 2007-08-14 Timothy Fowler Saccharification of cellulose by cloning and amplification of the .beta.-glucosidase gene of trichoderma reesei
ES2174820T3 (es) 1991-01-16 2002-11-16 Procter & Gamble Composiciones detergentes compactas con celulasa de alta actividad.
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
DE69209500T2 (de) 1991-04-30 1996-10-31 Procter & Gamble Gerüstsubstanzhaltige flüssigwaschmittel mit borsäure-polyolkomplex zur ptoteolytischen enzyminhibierung
KR100258460B1 (ko) 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
ATE262035T1 (de) 1992-10-06 2004-04-15 Novozymes As Zellulosevarianten
US5281526A (en) 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
DE69325981T2 (de) 1992-12-28 2000-02-03 Genencor International, Inc. Pullulanase, Mikroorganismen die sie produzieren, Verfahren zur Herstellung und ihre Anwendung
CA2155831C (en) 1993-02-11 2009-11-10 Richard L. Antrim Oxidatively stable alpha-amylase
EP0652946B1 (en) 1993-04-27 2005-01-26 Genencor International, Inc. New lipase variants for use in detergent applications
DK52393D0 (enExample) 1993-05-05 1993-05-05 Novo Nordisk As
DK76893D0 (enExample) 1993-06-28 1993-06-28 Novo Nordisk As
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
US5817495A (en) 1993-10-13 1998-10-06 Novo Nordisk A/S H2 O2 -stable peroxidase variants
DK114893D0 (enExample) 1993-10-14 1993-10-14 Novo Nordisk As
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
WO1995022615A1 (en) 1994-02-22 1995-08-24 Novo Nordisk A/S A method of preparing a variant of a lipolytic enzyme
DE69536145D1 (de) 1994-03-08 2011-04-07 Novozymes As Neuartige alkalische Zellulasen
WO1995030744A2 (en) 1994-05-04 1995-11-16 Genencor International Inc. Lipases with improved surfactant resistance
AU2884595A (en) 1994-06-20 1996-01-15 Unilever Plc Modified pseudomonas lipases and their use
AU2884695A (en) 1994-06-23 1996-01-19 Unilever Plc Modified pseudomonas lipases and their use
US6093563A (en) 1994-07-08 2000-07-25 Ibex Technologies R And D, Inc. Chondroitin lyase enzymes
ATE389012T1 (de) 1994-10-06 2008-03-15 Novozymes As Ein enzympräparat mit endoglucanase aktivität
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
US5827719A (en) 1994-10-26 1998-10-27 Novo Nordisk A/S Enzyme with lipolytic activity
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
CA2211316C (en) 1995-02-03 2013-10-01 Novo Nordisk A/S Method of designing alpha-amylase mutants with predetermined properties
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
CN102080070B (zh) 1995-03-17 2016-01-20 诺沃奇梅兹有限公司 新的内切葡聚糖酶
US5736499A (en) 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
AU6414196A (en) 1995-07-14 1997-02-18 Novo Nordisk A/S A modified enzyme with lipolytic activity
AU6513096A (en) 1995-07-19 1997-02-18 Novo Nordisk A/S Treatment of fabrics
JP4068142B2 (ja) 1995-08-11 2008-03-26 ノボザイムス アクティーゼルスカブ 新規の脂肪分解酵素
EP0904360B1 (en) 1996-04-30 2013-07-31 Novozymes A/S alpha-AMYLASE MUTANTS
US5763385A (en) 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
US6300065B1 (en) * 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US5958739A (en) 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
WO1998008940A1 (en) 1996-08-26 1998-03-05 Novo Nordisk A/S A novel endoglucanase
CN101085985B (zh) 1996-09-17 2012-05-16 诺沃奇梅兹有限公司 纤维素酶变体
DE69718351T2 (de) 1996-10-08 2003-11-20 Novozymes A/S, Bagsvaerd Diaminobenzoesäure derivate als farbstoffvorläufer
AU4773197A (en) 1996-11-04 1998-05-29 Novo Nordisk A/S Subtilase variants and compositions
ATE510910T1 (de) 1996-11-04 2011-06-15 Novozymes As Subtilase-varianten und verbindungen
US6159731A (en) 1997-02-12 2000-12-12 Massachusetts Institute Of Technology Daxx, a Fas-binding protein that activates JNK and apoptosis
US6008026A (en) 1997-07-11 1999-12-28 Genencor International, Inc. Mutant α-amylase having introduced therein a disulfide bond
WO1999019467A1 (en) 1997-10-13 1999-04-22 Novo Nordisk A/S α-AMYLASE MUTANTS
US6254645B1 (en) 1999-08-20 2001-07-03 Genencor International, Inc. Enzymatic modification of the surface of a polyester fiber or article
US6933140B1 (en) 1999-11-05 2005-08-23 Genencor International, Inc. Enzymes useful for changing the properties of polyester
US20030082595A1 (en) * 2001-08-03 2003-05-01 Bo Jiang Nucleic acids of aspergillus fumigatus encoding industrial enzymes and methods of use
DK1862626T3 (da) 2003-05-29 2011-12-12 Genencor Int Nye Trichoderma-gener
EP1675941B1 (en) 2003-06-25 2013-05-22 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
WO2005052148A2 (en) 2003-11-21 2005-06-09 Genencor International, Inc. Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch substrates
WO2005056783A1 (en) 2003-12-05 2005-06-23 Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Catalytic domains of beta(1,4)-galactosyltransferase i having altered metal ion specificity
CA2562171A1 (en) 2004-04-08 2005-11-24 Genencor International, Inc. Mutant .alpha.-amylases
DK2115143T3 (da) * 2007-02-07 2014-10-13 Danisco Us Inc Genencor Div Buttiauxella sp.-fytasevarianter med ændrede egenskaber
AU2009223508B2 (en) 2008-03-07 2013-08-01 Danisco Us Inc. Expression of catalase in Trichoderma
CA2801577C (en) 2010-06-03 2024-07-02 Stellenbosch University YEAST WITH SACCHAROLYTIC ENZYME EXPRESSION FOR BIOLOGICAL TRANSFORMATION CONSOLIDATED WITH STARCH AND CELLULOSE
JP5626854B2 (ja) * 2010-06-08 2014-11-19 国立大学法人神戸大学 エタノールの生産方法
EP2859109A2 (en) * 2012-08-16 2015-04-15 Danisco US Inc. Process for producing glucose from starch employing the aspergillus clavatus alpha-amylase and a pullulanase

Also Published As

Publication number Publication date
US20210047664A1 (en) 2021-02-18
JP2019115353A (ja) 2019-07-18
CA2893270C (en) 2024-01-02
WO2014092960A1 (en) 2014-06-19
US10767207B2 (en) 2020-09-08
JP2016500267A (ja) 2016-01-12
US20190177757A1 (en) 2019-06-13
CA2893270A1 (en) 2014-06-19
EP2931872A1 (en) 2015-10-21
EP2931872B1 (en) 2018-01-17
EP3321353A1 (en) 2018-05-16
US20160115509A1 (en) 2016-04-28

Similar Documents

Publication Publication Date Title
JP6560214B2 (ja) プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法
JP6499081B2 (ja) アスペルギルス・フミガタス(Aspergillusfumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichodermareesei)宿主細胞、及びその使用方法
CN104870631B (zh) 表达来自烟曲霉的葡糖淀粉酶的里氏木霉宿主细胞和其使用方法
US20130323798A1 (en) Method of using alpha-amylase from aspergillus clavatus for saccharification
CN103917642A (zh) 变体成麦芽六糖α-淀粉酶变体
JP2016501512A (ja) 糖化のためにタラロミセス・エメルソニ(TALAROMYCESEMERSONII)由来のα−アミラーゼを使用する方法
CN104903461A (zh) 将支链淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法
US20150218606A1 (en) Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
EP3060659B1 (en) Alpha-amylases from exiguobacterium, and methods of use, thereof
JP2015534456A (ja) アスペルギルス・クラバタス(Aspergillusclavatus)由来のαアミラーゼ及びイソアミラーゼを糖化に使用する方法
CN104769106A (zh) 使用来自埃默森篮状菌(TALAROMYCES EMERSONII)的α-淀粉酶进行糖化的方法
CN104903459A (zh) 将支链淀粉酶和来自烟曲霉的α-淀粉酶用于糖化的方法
WO2015021600A1 (en) Beta-amylase and methods of use
EP2922951B1 (en) Amylase with maltogenic properties
CN104903458A (zh) 将异淀粉酶和来自烟曲霉的α-淀粉酶用于糖化的方法
CN104903460A (zh) 将异淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法
CN104321437A (zh) 使用来自棒曲霉(ASPERGILLUS CLAVATUS)的α-淀粉酶进行糖化
WO2015094809A1 (en) Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof
CN104822829A (zh) 具有产麦芽糖特性的淀粉酶

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161104

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20161104

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170920

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20171003

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171030

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180403

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180625

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180903

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180928

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20190212

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20190314

R150 Certificate of patent or registration of utility model

Ref document number: 6499081

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250