CA2893270C - Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof - Google Patents

Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof Download PDF

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Publication number
CA2893270C
CA2893270C CA2893270A CA2893270A CA2893270C CA 2893270 C CA2893270 C CA 2893270C CA 2893270 A CA2893270 A CA 2893270A CA 2893270 A CA2893270 A CA 2893270A CA 2893270 C CA2893270 C CA 2893270C
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afgatr
starch
glucoamylase
amylase
glucose
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French (fr)
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CA2893270A1 (en
Inventor
Jing GE
Ling Hua
Sung Ho Lee
Jalsen LI
Jayarama K. Shetty
Zhongmei TANG
Bo Zhang
Kun ZHONG
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Danisco US Inc
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Danisco US Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
CA2893270A 2012-12-11 2013-11-21 Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof Active CA2893270C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2012086349 2012-12-11
CNPCT/CN2012/086349 2012-12-11
PCT/US2013/071154 WO2014092960A1 (en) 2012-12-11 2013-11-21 Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof

Publications (2)

Publication Number Publication Date
CA2893270A1 CA2893270A1 (en) 2014-06-19
CA2893270C true CA2893270C (en) 2024-01-02

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CA2893270A Active CA2893270C (en) 2012-12-11 2013-11-21 Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof

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US (3) US20160115509A1 (enExample)
EP (2) EP3321353A1 (enExample)
JP (2) JP6499081B2 (enExample)
CA (1) CA2893270C (enExample)
WO (1) WO2014092960A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2935920T3 (es) 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación
US9856498B2 (en) 2012-03-30 2018-01-02 Novozymes A/S Processes of producing fermentation products
EP3227430B1 (en) * 2014-12-01 2021-05-19 Danisco US Inc. Fungal host strains, dna constructs, and methods of use
CN107429267B (zh) * 2014-12-19 2021-07-16 丹尼斯科美国公司 葡糖淀粉酶共混物
EP3394279A1 (en) * 2015-12-21 2018-10-31 Danisco US Inc. Improved granular starch conversion enzymes and methods
EP3394278A1 (en) * 2015-12-21 2018-10-31 Danisco US Inc. Improved granular starch conversion enzymes and methods
EP3394277B1 (en) * 2015-12-21 2025-11-12 Danisco US Inc. Improved granular starch conversion enzymes and methods
US20210299229A1 (en) 2016-09-23 2021-09-30 Dupon Nutrition Biosciences Aps USE OF LOW pH ACTIVE ALPHA-1,4;/1,6-GLYCOSIDE HYDROLASES (GLCH) AS A FEED ADDITIVE FOR RUMINANTS TO ENHANCE STARCH DIGESTION
BR112019018506A2 (pt) 2017-03-07 2020-04-28 Danisco Us Inc glucoamilase termoestável e métodos de uso da mesma
WO2019047199A1 (en) 2017-09-11 2019-03-14 Danisco Us Inc. GLUCOAMYLASE AND METHODS OF USE THEREOF
ES3027937T3 (en) 2017-09-29 2025-06-17 Int N&H Denmark Aps Production of brewer's wort having increased fermentable sugars
CN114107360B (zh) * 2022-01-28 2022-04-08 中国农业科学院北京畜牧兽医研究所 一种通过干扰磷酸酶基因提高里氏木霉纤维素酶表达的方法

Family Cites Families (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
JPS57174089A (en) 1981-04-20 1982-10-26 Novo Industri As Chain dividing enzyme product
US5422267A (en) 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
ATE93541T1 (de) 1985-07-03 1993-09-15 Genencor Int Hybride polypeptide und verfahren zu deren herstellung.
JPH0697997B2 (ja) 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 新規の酵素的洗浄剤添加物
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
US4810414A (en) 1986-08-29 1989-03-07 Novo Industri A/S Enzymatic detergent additive
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
EP0305216B1 (en) 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DE68924654T2 (de) 1988-01-07 1996-04-04 Novonordisk As Spezifische Protease.
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
JP2728531B2 (ja) 1988-03-24 1998-03-18 ノボ ノルディスク アクティーゼルスカブ セルラーゼ調製品
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
US5055403A (en) 1989-06-26 1991-10-08 Enzyme Bio-Systems, Ltd. Thermoduric and aciduric pullulanase enzyme and method for its production
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
DE59101948D1 (de) 1990-04-14 1994-07-21 Kali Chemie Ag Alkalische bacillus-lipasen, hierfür codierende dna-sequenzen sowie bacilli, die diese lipasen produzieren.
AU639570B2 (en) 1990-05-09 1993-07-29 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
EP0548228B1 (en) 1990-09-13 1998-08-12 Novo Nordisk A/S Lipase variants
DE69126879T2 (de) 1990-09-28 1998-02-19 The Procter & Gamble Co., Cincinnati, Ohio Polyhydroxyfettsäureamidtenside zur erhöhung der enzymleistung
ATE223490T1 (de) 1990-12-10 2002-09-15 Genencor Int Verbesserte saccharifizierung von zellulose durch klonierung und vervielfältigung des beta- glukosidase genes aus trichoderma reesei
ES2174820T3 (es) 1991-01-16 2002-11-16 Procter & Gamble Composiciones detergentes compactas con celulasa de alta actividad.
PL170474B1 (pl) 1991-04-30 1996-12-31 Procter & Gamble C iekla kom pozycja detergentow a PL PL PL
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
KR100258460B1 (ko) 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
KR100303619B1 (ko) 1992-10-06 2001-11-22 피아 스타르 셀룰라제변이체
US5281526A (en) 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
DE69325981T2 (de) 1992-12-28 2000-02-03 Genencor International, Inc. Pullulanase, Mikroorganismen die sie produzieren, Verfahren zur Herstellung und ihre Anwendung
CN1104499C (zh) 1993-02-11 2003-04-02 吉恩康国际有限公司 氧化作用稳定的α-淀粉酶
JP3618748B2 (ja) 1993-04-27 2005-02-09 ジェネンコー インターナショナル インコーポレイテッド 洗剤に使用する新しいリパーゼ変異体
DK52393D0 (enExample) 1993-05-05 1993-05-05 Novo Nordisk As
DK76893D0 (enExample) 1993-06-28 1993-06-28 Novo Nordisk As
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
CN1133062A (zh) 1993-10-13 1996-10-09 诺沃挪第克公司 对过氧化氢稳定的过氧化物酶变体
DK114893D0 (enExample) 1993-10-14 1993-10-14 Novo Nordisk As
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
WO1995022615A1 (en) 1994-02-22 1995-08-24 Novo Nordisk A/S A method of preparing a variant of a lipolytic enzyme
AU1890095A (en) 1994-03-08 1995-09-25 Novo Nordisk A/S Novel alkaline cellulases
JP3851656B2 (ja) 1994-05-04 2006-11-29 ジェネンコア インターナショナル インコーポレーテッド 改善された界面活性剤耐性を有するリパーゼ
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
WO1996000292A1 (en) 1994-06-23 1996-01-04 Unilever N.V. Modified pseudomonas lipases and their use
US6093563A (en) 1994-07-08 2000-07-25 Ibex Technologies R And D, Inc. Chondroitin lyase enzymes
ATE389012T1 (de) 1994-10-06 2008-03-15 Novozymes As Ein enzympräparat mit endoglucanase aktivität
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
WO1996013580A1 (en) 1994-10-26 1996-05-09 Novo Nordisk A/S An enzyme with lipolytic activity
DK2199378T3 (da) 1995-02-03 2012-10-29 Novozymes As alfa-amylase mutanter
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
WO1996029397A1 (en) 1995-03-17 1996-09-26 Novo Nordisk A/S Novel endoglucanases
US5736499A (en) 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
ATE282087T1 (de) 1995-07-14 2004-11-15 Novozymes As Modifiziertes enzym mit lipolytischer aktivität
AU6513096A (en) 1995-07-19 1997-02-18 Novo Nordisk A/S Treatment of fabrics
AU6655196A (en) 1995-08-11 1997-03-12 Novo Nordisk A/S Novel lipolytic enzymes
EP0904360B1 (en) 1996-04-30 2013-07-31 Novozymes A/S alpha-AMYLASE MUTANTS
US5763385A (en) 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
US6300065B1 (en) * 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US5958739A (en) 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
WO1998008940A1 (en) 1996-08-26 1998-03-05 Novo Nordisk A/S A novel endoglucanase
EP1726644A1 (en) 1996-09-17 2006-11-29 Novozymes A/S Cellulase variants
WO1998015257A1 (en) 1996-10-08 1998-04-16 Novo Nordisk A/S Diaminobenzoic acid derivatives as dye precursors
ATE409743T1 (de) 1996-11-04 2008-10-15 Novozymes As Subtilase varianten und verbindungen
WO1998020115A1 (en) 1996-11-04 1998-05-14 Novo Nordisk A/S Subtilase variants and compositions
US6159731A (en) 1997-02-12 2000-12-12 Massachusetts Institute Of Technology Daxx, a Fas-binding protein that activates JNK and apoptosis
US6008026A (en) 1997-07-11 1999-12-28 Genencor International, Inc. Mutant α-amylase having introduced therein a disulfide bond
DK2206768T3 (en) 1997-10-13 2015-06-29 Novozymes As Alfa-amylasemutanter
US6254645B1 (en) 1999-08-20 2001-07-03 Genencor International, Inc. Enzymatic modification of the surface of a polyester fiber or article
US6933140B1 (en) 1999-11-05 2005-08-23 Genencor International, Inc. Enzymes useful for changing the properties of polyester
WO2003012071A2 (en) * 2001-08-03 2003-02-13 Elitra Pharmaceuticals, Inc. Nucleic acids of aspergillus fumigatus encoding industrial enzymes and methods of use
EP2261326B1 (en) 2003-05-29 2017-03-29 Danisco US Inc. Novel Trichoderma genes
WO2005001064A2 (en) 2003-06-25 2005-01-06 Novozymes A/S Polypeptides having alpha-amylase activity and polypeptides encoding same
CN102174490B (zh) 2003-11-21 2016-09-28 丹尼斯科美国公司 颗粒淀粉水解酶在木霉菌中的表达和从颗粒淀粉底物产生葡萄糖的方法
US8703459B2 (en) 2003-12-05 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Catalytic domains of beta(1,4)-galactosyltransferase I having altered metal ion specificity
CN1938422A (zh) 2004-04-08 2007-03-28 金克克国际有限公司 突变体α-淀粉酶
WO2008097619A2 (en) * 2007-02-07 2008-08-14 Danisco Us, Inc., Genencor Division Variant buttiauxella sp. phytases having altered properties
BRPI0910252A2 (pt) 2008-03-07 2015-09-01 Danisco Us Inc Expressão de catalise em trichoderma
EP2576762A4 (en) 2010-06-03 2013-12-04 Mascoma Corp YEAST FOR THE EXPRESSION OF SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOVERIC PROCESSING USING STARCH AND CELLULOSE
JP5626854B2 (ja) * 2010-06-08 2014-11-19 国立大学法人神戸大学 エタノールの生産方法
MX2015001818A (es) * 2012-08-16 2015-05-07 Danisco Inc Proceso para producir glucosa a partir de almidon con el uso de la alfa-amilasa de aspergillus clavatus y una pululanasa.

Also Published As

Publication number Publication date
JP2019115353A (ja) 2019-07-18
WO2014092960A1 (en) 2014-06-19
EP2931872B1 (en) 2018-01-17
US20160115509A1 (en) 2016-04-28
EP3321353A1 (en) 2018-05-16
US20190177757A1 (en) 2019-06-13
US10767207B2 (en) 2020-09-08
JP2016500267A (ja) 2016-01-12
EP2931872A1 (en) 2015-10-21
US20210047664A1 (en) 2021-02-18
CA2893270A1 (en) 2014-06-19
JP6499081B2 (ja) 2019-04-10

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