CA2893270C - Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof - Google Patents

Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof Download PDF

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Publication number
CA2893270C
CA2893270C CA2893270A CA2893270A CA2893270C CA 2893270 C CA2893270 C CA 2893270C CA 2893270 A CA2893270 A CA 2893270A CA 2893270 A CA2893270 A CA 2893270A CA 2893270 C CA2893270 C CA 2893270C
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Prior art keywords
afgatr
starch
glucoamylase
amylase
glucose
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English (en)
French (fr)
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CA2893270A1 (en
Inventor
Jing GE
Ling Hua
Sung Ho Lee
Jalsen LI
Jayarama K. Shetty
Zhongmei TANG
Bo Zhang
Kun ZHONG
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Danisco US Inc
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Danisco US Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
CA2893270A 2012-12-11 2013-11-21 Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof Active CA2893270C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2012086349 2012-12-11
CNPCT/CN2012/086349 2012-12-11
PCT/US2013/071154 WO2014092960A1 (en) 2012-12-11 2013-11-21 Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof

Publications (2)

Publication Number Publication Date
CA2893270A1 CA2893270A1 (en) 2014-06-19
CA2893270C true CA2893270C (en) 2024-01-02

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CA2893270A Active CA2893270C (en) 2012-12-11 2013-11-21 Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof

Country Status (5)

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US (3) US20160115509A1 (enExample)
EP (2) EP2931872B1 (enExample)
JP (2) JP6499081B2 (enExample)
CA (1) CA2893270C (enExample)
WO (1) WO2014092960A1 (enExample)

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EP3227430B1 (en) * 2014-12-01 2021-05-19 Danisco US Inc. Fungal host strains, dna constructs, and methods of use
US10612058B2 (en) * 2014-12-19 2020-04-07 Danisco Us Inc Methods for saccharifying a starch substrate
US20190010473A1 (en) * 2015-12-21 2019-01-10 Danisco Us Inc. Improved granular starch conversion enzymes and methods
CN109072267A (zh) * 2015-12-21 2018-12-21 丹尼斯科美国公司 改善的颗粒状淀粉转化酶和方法
BR112018012617A2 (pt) * 2015-12-21 2018-12-04 C Koops Bart enzimas e métodos de conversão de amido granular aprimorada
MX2019003250A (es) * 2016-09-23 2019-08-29 Dupont Nutrition Biosci Aps Uso de alfa-1,4/1,6-glucosido hidrolasas activas a ph bajo como aditivo alimentario para rumiantes para mejorar la digestion de almidon.
EP3592849A1 (en) 2017-03-07 2020-01-15 Danisco US Inc. Thermostable glucoamylase and methods of use, thereof
WO2019047199A1 (en) 2017-09-11 2019-03-14 Danisco Us Inc. GLUCOAMYLASE AND METHODS OF USE THEREOF
EP3673039B1 (en) * 2017-09-29 2025-03-19 International N&H Denmark ApS Production of brewer's wort having increased fermentable sugars
CN114107360B (zh) * 2022-01-28 2022-04-08 中国农业科学院北京畜牧兽医研究所 一种通过干扰磷酸酶基因提高里氏木霉纤维素酶表达的方法

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Also Published As

Publication number Publication date
US20210047664A1 (en) 2021-02-18
JP2019115353A (ja) 2019-07-18
WO2014092960A1 (en) 2014-06-19
US10767207B2 (en) 2020-09-08
JP2016500267A (ja) 2016-01-12
US20190177757A1 (en) 2019-06-13
CA2893270A1 (en) 2014-06-19
EP2931872A1 (en) 2015-10-21
EP2931872B1 (en) 2018-01-17
EP3321353A1 (en) 2018-05-16
JP6499081B2 (ja) 2019-04-10
US20160115509A1 (en) 2016-04-28

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