JP6189581B2 - 幹細胞の分化のための方法および組成物 - Google Patents

幹細胞の分化のための方法および組成物 Download PDF

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JP6189581B2
JP6189581B2 JP2011551266A JP2011551266A JP6189581B2 JP 6189581 B2 JP6189581 B2 JP 6189581B2 JP 2011551266 A JP2011551266 A JP 2011551266A JP 2011551266 A JP2011551266 A JP 2011551266A JP 6189581 B2 JP6189581 B2 JP 6189581B2
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JP2012518415A (ja
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クリスティン エー. ダイ,
クリスティン エー. ダイ,
ピーター フールケン,
ピーター フールケン,
ジョルジア サルバジオット,
ジョルジア サルバジオット,
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セルラー ダイナミクス インターナショナル, インコーポレイテッド
セルラー ダイナミクス インターナショナル, インコーポレイテッド
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Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150088336A (ko) 2007-09-28 2015-07-31 안트로제네시스 코포레이션 인간 태반 관류액 및 인간 태반-유래 중간체 천연 킬러 세포를 사용한 종양 억제 방법
WO2010120785A2 (en) * 2009-04-13 2010-10-21 The Regents Of The University Of California Methods and compositions for stem cell cultures
US9428735B2 (en) 2010-02-25 2016-08-30 The Johns Hopkins University Smooth muscle-like cells (SMLCs) dervided from human pluripotent stem cells
US20130071927A1 (en) * 2010-05-05 2013-03-21 Sydney Ivf Limited Media and methods for cell culture
US20120064040A1 (en) * 2010-06-03 2012-03-15 The Regents Of The University Of California Serum free culture medium and supplement
ES2666746T3 (es) 2010-07-13 2018-05-07 Anthrogenesis Corporation Métodos para generar linfocitos citolíticos naturales
US8765470B2 (en) 2010-08-04 2014-07-01 Cellular Dynamics International, Inc. Reprogramming immortalized B-cells to induced pluripotent stem cells
CN102985531B (zh) * 2010-09-10 2019-04-23 国立大学法人东京大学 涉及由多能干细胞向血细胞分化的培养方法
AU2011328087A1 (en) 2010-11-09 2013-06-06 Lonza Cologne Gmbh Method for controlling binding of cells to a substrate
JP6163104B2 (ja) 2010-11-15 2017-07-12 アクセラレイテッド・バイオサイエンシズ・コーポレーション ヒト・トロホブラスト幹細胞からの神経幹細胞の生成
US20140045265A1 (en) * 2011-02-16 2014-02-13 Salk Institute For Biological Studies Robust and efficient differentiation of human pluripotent stem cells to multipotent vascular progenitors
US9499789B2 (en) 2011-02-23 2016-11-22 Kyoto University Method for producing dendritic cells from pluripotent stem cells
US20120258439A1 (en) * 2011-04-11 2012-10-11 Ali Bin M Abdullah Jaffar Protein-Free Culture Media Products
HRP20171937T1 (hr) * 2011-09-29 2018-02-09 Cellsnap, Llc Pripravci za i postupci ispitivanja toksigenosti
KR20240019378A (ko) * 2011-12-01 2024-02-14 뉴욕 스템 셀 파운데이션, 인코포레이티드 유도 다능성 줄기 세포 또는 분화된 세포를 제조하기 위한 자동화 시스템
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
WO2013086029A1 (en) 2011-12-05 2013-06-13 Primorigen Biosciences Inc. Compositions and methods for differentiating pluripotent stem cells into primitive blood cells and uses thereof
WO2013130820A1 (en) * 2012-02-29 2013-09-06 The Johns Hopkins University Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation
SG11201502375QA (en) 2012-11-21 2015-05-28 Merz Pharma Gmbh & Co Kgaa Means and methods for determination of botulinum neurotoxin biological activity
CA2891542C (en) 2012-11-30 2021-12-14 Accelerated Biosciences Corp. Pancreatic progenitor cells expressing betatrophin and insulin
WO2014100779A1 (en) * 2012-12-21 2014-06-26 Advanced Cell Technology, Inc. Methods ofr production of platelets from pluripotent stem cells and compositions thereof
WO2014123879A1 (en) 2013-02-05 2014-08-14 Anthrogenesis Corporation Natural killer cells from placenta
US9994825B2 (en) 2013-03-15 2018-06-12 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
EP2777703B1 (en) * 2013-03-15 2016-09-14 Lonza Cologne GmbH Compositions and methods for enhancing the therapeutic potential of stem cells
US9506037B2 (en) 2013-03-15 2016-11-29 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
SG11201510685UA (en) 2013-06-28 2016-01-28 Merz Pharma Gmbh & Co Kgaa Means and methods for the determination of the biological activity of neurotoxin polypeptides in cells
WO2015097920A1 (ja) * 2013-12-27 2015-07-02 京都府公立大学法人 角膜内皮細胞の細胞治療併用剤
WO2015119642A1 (en) * 2014-02-10 2015-08-13 The Johns Hopkins University Low oxygen tension enhances endothelial fate of human pluripotent stem cells
AU2015220915B2 (en) 2014-02-19 2020-09-17 Merz Pharma Gmbh & Co. Kgaa Gangliosides for standardizing and increasing the sensitivity of cells to Botulinum neurotoxins in in vitro test systems
US20170044500A1 (en) 2014-04-24 2017-02-16 Board Of Regents, The University Of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
EP3224347A4 (en) 2014-11-26 2018-08-15 Accelerated BioSciences Corp. Induced hepatocytes and uses thereof
JP6772138B2 (ja) 2014-12-19 2020-10-21 メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー 細胞内のBoNT/Eの生物学的活性を決定するための手段及び方法
JP6800859B2 (ja) 2015-01-26 2020-12-16 フェイト セラピューティクス,インコーポレイテッド 造血細胞分化を誘導するための方法および組成物
TWI704156B (zh) 2015-03-04 2020-09-11 德商曼茲法瑪股份有限公司 用於增進肉毒桿菌神經毒素進入細胞之特定攝取的方法
CN115806940A (zh) 2015-11-04 2023-03-17 菲特治疗公司 多能细胞的基因组工程改造
JP7534070B2 (ja) * 2015-11-04 2024-08-14 フェイト セラピューティクス,インコーポレイテッド 造血細胞分化を誘導するための方法および組成物
DK3460042T3 (da) 2016-05-18 2024-03-11 Univ Keio Celledyrkningsmedium til dyrkning af et organoid, dyrkningsfremgnagsmåde og organoid
KR102592176B1 (ko) 2018-01-05 2023-10-20 플레이틀렛 바이오제네시스, 인크. 거핵구 생성을 위한 조성물 및 방법
JP2021529770A (ja) 2018-06-29 2021-11-04 プレートレット バイオジェネシス, インコーポレイテッド 薬物送達のための組成物およびその使用方法
AU2019336221B2 (en) * 2018-09-07 2025-05-15 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
CN110438065B (zh) * 2019-07-25 2020-06-05 中山大学 一种诱导人诱导性多能干细胞分化为内皮祖细胞的方法
AU2020381537A1 (en) * 2019-11-14 2022-06-09 Amgen Inc. Hematopoietic precursor cell production
JPWO2022039250A1 (enExample) * 2020-08-21 2022-02-24
WO2024018018A1 (fr) 2022-07-21 2024-01-25 Etablissement Francais Du Sang Utilisation d'un composé vitisine pour la production de cellules hématopoïétiques
WO2025126208A1 (en) * 2023-12-12 2025-06-19 Accellta Ltd. Media for maintaining, differentiating, or both, pluripotent stem cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3065499D1 (en) 1979-04-11 1983-12-15 Mitsui Petrochemical Ind Process for producing spherical carrier particles for olefin polymerisation catalyst, catalyst comprising such carrier particles, and use of such catalyst in olefin polymerisation
GB9807935D0 (en) 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
WO1998030679A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
WO2000057913A1 (en) 1999-03-25 2000-10-05 Welfide Corporation Preventives/remedies for interstitial pneumonia and pulmonary fibrosis
WO2000078351A1 (en) 1999-06-18 2000-12-28 Mitsubishi Pharma Corporation Osteogenesis promoters
JP4563573B2 (ja) * 2000-12-07 2010-10-13 株式会社カネカ 抗原およびこの抗原を識別するモノクローナル抗体
ES2298497T3 (es) 2002-01-23 2008-05-16 Bayer Pharmaceuticals Corporation Inhibidores de quinasa rho.
US6664266B2 (en) 2002-03-14 2003-12-16 Children's Medical Center Corporation Axon regeneration with PKC inhibitiors
GB0206860D0 (en) 2002-03-22 2002-05-01 Glaxo Group Ltd Compounds
AU2003234186B2 (en) 2002-04-22 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Peptide inhibitors of protein kinase C Gamma for pain management
WO2004029200A2 (en) 2002-09-26 2004-04-08 Children's Medical Center Corporation Method of enhancing proliferation and/or hematopoietic differentiation of stem cells
WO2006029197A1 (en) 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
MX2007002389A (es) 2004-09-08 2009-02-12 Wisconsin Alumni Res Found Cultivo de celulas progenitoras embrionarias humanas.
KR20070073932A (ko) 2004-11-01 2007-07-10 위스콘신 얼럼나이 리서어치 화운데이션 줄기 세포 유래 혈소판
WO2008011176A2 (en) 2006-07-21 2008-01-24 Northwestern University Myosin light chain kinase inhibitor compounds, compositions and related methods of use
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
CA2675445C (en) * 2007-01-17 2014-11-04 Wisconsin Alumni Research Foundation Improved culture of stem cells
KR101516833B1 (ko) 2007-03-23 2015-05-07 위스콘신 얼럼나이 리서어치 화운데이션 체세포 재프로그래밍
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US8815585B2 (en) 2007-06-29 2014-08-26 Cellular Dynamics International, Inc. Automated method and apparatus for embryonic stem cell culture
US8513012B2 (en) * 2008-05-02 2013-08-20 Cellular Dynamics International, Inc. Method for production of mast cells from stem cells

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US20100216181A1 (en) 2010-08-26
CA2753208A1 (en) 2010-08-26
WO2010096746A1 (en) 2010-08-26
US8557580B2 (en) 2013-10-15
CA2753208C (en) 2018-08-21
JP6691509B2 (ja) 2020-04-28
JP2017148079A (ja) 2017-08-31
JP2012518415A (ja) 2012-08-16
JP2016019543A (ja) 2016-02-04
EP2398897B1 (en) 2017-06-28
EP2398897A1 (en) 2011-12-28

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