JP6189581B2 - 幹細胞の分化のための方法および組成物 - Google Patents

幹細胞の分化のための方法および組成物 Download PDF

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JP6189581B2
JP6189581B2 JP2011551266A JP2011551266A JP6189581B2 JP 6189581 B2 JP6189581 B2 JP 6189581B2 JP 2011551266 A JP2011551266 A JP 2011551266A JP 2011551266 A JP2011551266 A JP 2011551266A JP 6189581 B2 JP6189581 B2 JP 6189581B2
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JP2012518415A (ja
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クリスティン エー. ダイ,
クリスティン エー. ダイ,
ピーター フールケン,
ピーター フールケン,
ジョルジア サルバジオット,
ジョルジア サルバジオット,
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セルラー ダイナミクス インターナショナル, インコーポレイテッド
セルラー ダイナミクス インターナショナル, インコーポレイテッド
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PCT/US2010/024881 WO2010096746A1 (en) 2009-02-20 2010-02-22 Methods and compositions for the differentiation of stem cells

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JP2015213836A Division JP2016019543A (ja) 2009-02-20 2015-10-30 幹細胞の分化のための方法および組成物
JP2017113273A Division JP6691509B2 (ja) 2009-02-20 2017-06-08 幹細胞の分化のための方法および組成物

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Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2700617C (en) 2007-09-28 2018-11-06 Celgene Cellular Therapeutics Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells
KR20120017019A (ko) * 2009-04-13 2012-02-27 더 리전트 오브 더 유니버시티 오브 캘리포니아 줄기 세포 배양 방법 및 조성물
US9428735B2 (en) 2010-02-25 2016-08-30 The Johns Hopkins University Smooth muscle-like cells (SMLCs) dervided from human pluripotent stem cells
US20130071927A1 (en) * 2010-05-05 2013-03-21 Sydney Ivf Limited Media and methods for cell culture
US20120064040A1 (en) * 2010-06-03 2012-03-15 The Regents Of The University Of California Serum free culture medium and supplement
KR20180099915A (ko) * 2010-07-13 2018-09-05 안트로제네시스 코포레이션 천연 킬러 세포의 생성 방법
US8765470B2 (en) 2010-08-04 2014-07-01 Cellular Dynamics International, Inc. Reprogramming immortalized B-cells to induced pluripotent stem cells
EP2615165B1 (en) * 2010-09-10 2016-08-03 The University of Tokyo Culture method related to differentiation of pluripotent stem cells into blood cells
JP2013541344A (ja) 2010-11-09 2013-11-14 ロンザ ケルン ゲーエムベーハー 支持体への細胞の結合を調節するための方法
WO2012068170A2 (en) 2010-11-15 2012-05-24 Jau-Nan Lee Generation of neural stem cells from human trophoblast stem cells
WO2012112776A2 (en) * 2011-02-16 2012-08-23 Salk Institute For Biological Studies Robust and efficient differentiation of human pluripotent stem cells to multipotent vascular progenitors
US9499789B2 (en) 2011-02-23 2016-11-22 Kyoto University Method for producing dendritic cells from pluripotent stem cells
EP2697360A1 (en) * 2011-04-11 2014-02-19 Jaffar Ali Bin M. Abdullah Protein-free culture media products
KR101640694B1 (ko) * 2011-09-29 2016-07-18 셀스냅, 엘엘씨 독소생산능 시험용 조성물 및 방법
AU2012345676B2 (en) * 2011-12-01 2018-10-04 New York Stem Cell Foundation, Inc. Automated system for producing induced pluripotent stem cells or differentiated cells
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
US9428732B2 (en) 2011-12-05 2016-08-30 Primorigen Biosciences, Inc. Compositions and methods for differentiating pluripotent stem cells into primitive blood cells and uses thereof
WO2013130820A1 (en) * 2012-02-29 2013-09-06 The Johns Hopkins University Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation
US20160289731A1 (en) 2012-11-21 2016-10-06 Merz Pharma Gmbh & Co, Kgaa Means and methods for determination of botulinum neurotoxin biological activity
GB2523696B (en) 2012-11-30 2017-12-27 Accelerated Biosciences Corp Methods of differentiating stem cells to pancreatic progenitor cells by modulating miR-124
CN114558032B (zh) * 2012-12-21 2025-07-29 安斯泰来再生医药协会 由多能干细胞制备血小板的方法及其组合物
AU2014215458A1 (en) 2013-02-05 2015-08-13 Anthrogenesis Corporation Natural killer cells from placenta
EP2777703B1 (en) * 2013-03-15 2016-09-14 Lonza Cologne GmbH Compositions and methods for enhancing the therapeutic potential of stem cells
US9506037B2 (en) 2013-03-15 2016-11-29 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
US9994825B2 (en) 2013-03-15 2018-06-12 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
JP6608357B2 (ja) 2013-06-28 2019-11-20 メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー 細胞において神経毒ポリペプチドの生物活性を決定する手段及び方法
US10959997B2 (en) 2013-12-27 2021-03-30 Kyoto Prefectural Public University Corporation Combined agent for cell therapy of corneal endothelial cell
WO2015119642A1 (en) * 2014-02-10 2015-08-13 The Johns Hopkins University Low oxygen tension enhances endothelial fate of human pluripotent stem cells
AU2015220915B2 (en) 2014-02-19 2020-09-17 Merz Pharma Gmbh & Co. Kgaa Gangliosides for standardizing and increasing the sensitivity of cells to Botulinum neurotoxins in in vitro test systems
WO2015164740A1 (en) 2014-04-24 2015-10-29 Board Of Regents, The University Of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
SG11201704291QA (en) 2014-11-26 2017-06-29 Accelerated Biosciences Corp Induced hepatocytes and uses thereof
AU2015367329B2 (en) 2014-12-19 2021-02-18 Merz Pharma Gmbh & Co. Kgaa Means and methods for the determination of the biological activity of BoNT/E in cells
WO2016123100A1 (en) 2015-01-26 2016-08-04 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
TWI704156B (zh) 2015-03-04 2020-09-11 德商曼茲法瑪股份有限公司 用於增進肉毒桿菌神經毒素進入細胞之特定攝取的方法
WO2017078807A1 (en) 2015-11-04 2017-05-11 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
CN108368520B (zh) 2015-11-04 2023-01-17 菲特治疗公司 多能细胞的基因组工程改造
ES2973213T3 (es) 2016-05-18 2024-06-19 Univ Keio Medio de cultivo celular para cultivo de organoide, método de cultivo y organoide
MX2020007071A (es) 2018-01-05 2020-11-11 Platelet Biogenesis Inc Composiciones y metodos para producir megacariocitos.
EP3813853A4 (en) 2018-06-29 2022-04-06 Platelet Biogenesis, Inc. DELIVERY COMPOSITIONS AND METHODS OF USE
AU2019336221B2 (en) 2018-09-07 2025-05-15 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
CN110438065B (zh) * 2019-07-25 2020-06-05 中山大学 一种诱导人诱导性多能干细胞分化为内皮祖细胞的方法
EP4058564A1 (en) * 2019-11-14 2022-09-21 Amgen Inc. Hematopoietic precursor cell production
WO2022039250A1 (ja) * 2020-08-21 2022-02-24 Jsr株式会社 ヒト人工多能性幹細胞の培養方法、ヒト人工多能性幹細胞培養物、及び脳オルガノイドの製造方法
EP4558619A1 (fr) 2022-07-21 2025-05-28 Etablissement Français du Sang Utilisation d'un composé vitisine pour la production de cellules hématopoïétiques
WO2025126208A1 (en) * 2023-12-12 2025-06-19 Accellta Ltd. Media for maintaining, differentiating, or both, pluripotent stem cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0018737B1 (en) 1979-04-11 1983-11-09 Mitsui Petrochemical Industries, Ltd. Process for producing spherical carrier particles for olefin polymerisation catalyst, catalyst comprising such carrier particles, and use of such catalyst in olefin polymerisation
GB9807935D0 (en) 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
AU5734998A (en) 1997-01-10 1998-08-03 Life Technologies, Inc. Embryonic stem cell serum replacement
US6844354B1 (en) 1999-03-25 2005-01-18 Mitsubishi Pharma Corporation Agent for prophylaxis and treatment of interstitial pneumonia and pulmonary fibrosis
AU5250400A (en) 1999-06-18 2001-01-09 Mitsubishi Pharma Corporation Osteogenesis promoters
JP4563573B2 (ja) * 2000-12-07 2010-10-13 株式会社カネカ 抗原およびこの抗原を識別するモノクローナル抗体
MXPA04007196A (es) 2002-01-23 2005-06-08 Bayer Pharmaceuticals Corp Inhibidores de rho-quinasa.
US6664266B2 (en) 2002-03-14 2003-12-16 Children's Medical Center Corporation Axon regeneration with PKC inhibitiors
GB0206860D0 (en) 2002-03-22 2002-05-01 Glaxo Group Ltd Compounds
US7393835B2 (en) 2002-04-22 2008-07-01 The Board Of Trustees Of The Leland Stanford Junior University Peptide inhibitors of protein kinase C
AU2003273332A1 (en) 2002-09-26 2004-04-19 Children's Medical Center Corporation Method of enhancing proliferation and/or hematopoietic differentiation of stem cells
BRPI0514778B1 (pt) 2004-09-08 2018-07-10 Wisconsin Alumni Research Foundation Meio para cultivar células tronco embrionárias
DK3196296T3 (en) 2004-09-08 2019-02-04 Wisconsin Alumini Res Foundation Cultivation of human embryonic stem cells
AU2005302258B2 (en) 2004-11-01 2010-10-21 Wisconsin Alumni Research Foundation Platelets from stem cells
WO2008011176A2 (en) 2006-07-21 2008-01-24 Northwestern University Myosin light chain kinase inhibitor compounds, compositions and related methods of use
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
CA2675445C (en) * 2007-01-17 2014-11-04 Wisconsin Alumni Research Foundation Improved culture of stem cells
EP3399025B1 (en) 2007-03-23 2025-05-07 Wisconsin Alumni Research Foundation Somatic cell reprogramming
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
DK2173863T3 (en) 2007-06-29 2019-01-21 Fujifilm Cellular Dynamics Inc Automated method and apparatus for embryonic stem cell culture
US8513012B2 (en) * 2008-05-02 2013-08-20 Cellular Dynamics International, Inc. Method for production of mast cells from stem cells

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JP2012518415A (ja) 2012-08-16
EP2398897B1 (en) 2017-06-28
JP2017148079A (ja) 2017-08-31
EP2398897A1 (en) 2011-12-28
US20100216181A1 (en) 2010-08-26
US8557580B2 (en) 2013-10-15
CA2753208A1 (en) 2010-08-26
JP2016019543A (ja) 2016-02-04
CA2753208C (en) 2018-08-21
JP6691509B2 (ja) 2020-04-28
WO2010096746A1 (en) 2010-08-26

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