JP6185978B2 - ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物 - Google Patents

ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物 Download PDF

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JP6185978B2
JP6185978B2 JP2015500639A JP2015500639A JP6185978B2 JP 6185978 B2 JP6185978 B2 JP 6185978B2 JP 2015500639 A JP2015500639 A JP 2015500639A JP 2015500639 A JP2015500639 A JP 2015500639A JP 6185978 B2 JP6185978 B2 JP 6185978B2
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human
light chain
sequence
histidine
chain variable
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JP2015510767A5 (enExample
JP2015510767A (ja
Inventor
ジョン ミクウィルター，
ジョン ミクウィルター，
リン マクドナルド，
リン マクドナルド，
アンドリュー ジェイ． マーフィー，
アンドリュー ジェイ． マーフィー，
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2015500639A 2012-03-16 2013-03-15 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物 Active JP6185978B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261611950P 2012-03-16 2012-03-16
US61/611,950 2012-03-16
US201261736930P 2012-12-13 2012-12-13
US61/736,930 2012-12-13
PCT/US2013/031823 WO2013138680A1 (en) 2012-03-16 2013-03-15 Histidine engineered light chain antibodies and genetically modified non-human animals for generating the same

Related Child Applications (1)

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JP2017052358A Division JP6574802B2 (ja) 2012-03-16 2017-03-17 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物

Publications (3)

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JP2015510767A JP2015510767A (ja) 2015-04-13
JP2015510767A5 JP2015510767A5 (enExample) 2016-05-12
JP6185978B2 true JP6185978B2 (ja) 2017-08-23

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JP2015500639A Active JP6185978B2 (ja) 2012-03-16 2013-03-15 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物
JP2017052358A Active JP6574802B2 (ja) 2012-03-16 2017-03-17 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物
JP2019029627A Active JP7304169B2 (ja) 2012-03-16 2019-02-21 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物
JP2020066673A Active JP7199398B2 (ja) 2012-03-16 2020-04-02 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物
JP2021183079A Withdrawn JP2022017547A (ja) 2012-03-16 2021-11-10 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物

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JP2017052358A Active JP6574802B2 (ja) 2012-03-16 2017-03-17 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物
JP2019029627A Active JP7304169B2 (ja) 2012-03-16 2019-02-21 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物
JP2020066673A Active JP7199398B2 (ja) 2012-03-16 2020-04-02 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物
JP2021183079A Withdrawn JP2022017547A (ja) 2012-03-16 2021-11-10 ヒスチジン操作された軽鎖抗体およびこれを作製するための遺伝子改変された非ヒト動物

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US (3) US9334334B2 (enExample)
EP (4) EP3348140B1 (enExample)
JP (5) JP6185978B2 (enExample)
KR (1) KR102228296B1 (enExample)
CN (3) CN107827979A (enExample)
AU (3) AU2013204579B2 (enExample)
BR (1) BR112014022850A2 (enExample)
CA (1) CA2865643A1 (enExample)
CY (2) CY1120599T1 (enExample)
DK (2) DK2825036T3 (enExample)
ES (3) ES2665793T3 (enExample)
HR (2) HRP20181228T1 (enExample)
HU (3) HUE037659T2 (enExample)
IL (5) IL234355B (enExample)
LT (3) LT3348140T (enExample)
MX (2) MX381210B (enExample)
MY (1) MY172730A (enExample)
NZ (1) NZ629639A (enExample)
PL (2) PL2883449T3 (enExample)
PT (3) PT3348140T (enExample)
RS (2) RS57414B1 (enExample)
RU (2) RU2017135754A (enExample)
SG (2) SG11201405165UA (enExample)
SI (3) SI2883449T1 (enExample)
SM (2) SMT201800395T1 (enExample)
WO (1) WO2013138680A1 (enExample)

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