JP5907891B2 - ツマジロクサヨトウ(fallarmyworm)およびユーロピアンコーンボーラー(Europeancornborer)を防除するための殺虫性タンパク質の組み合わせならびに害虫抵抗性管理のための方法 - Google Patents
ツマジロクサヨトウ(fallarmyworm)およびユーロピアンコーンボーラー(Europeancornborer)を防除するための殺虫性タンパク質の組み合わせならびに害虫抵抗性管理のための方法 Download PDFInfo
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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Description
構造化された緩衝帯:コーンベルトにおける非鱗翅目(lepidopteran)Btトウモロコシ緩衝帯、20%
コットンベルトにおける非鱗翅目(lepidopteran)Bt緩衝帯、50%
ブロック
内部(すなわち、Bt圃場内)
外部(すなわち、任意交配を最大限に高めるための、Bt圃場の1/2マイル(可能であれば1/4マイル)以内にある別の圃場)
圃場内帯状地(strip)
帯状地は、幼生移動の効果を低減するために少なくとも4列幅(好ましくは6列)である必要がある」
「コーンボーラーIRMの要件:
− 緩衝帯雑種をトウモロコシ畑の少なくとも20%に植えること
− ワタ生産区域において、緩衝帯は50%でなければならない
− 緩衝帯雑種の1/2マイル以内に植えなければならない
− 緩衝帯はBt圃場内に帯状地として設置することができる;緩衝帯は、少なくとも4列幅でなければならない
− 標的昆虫に対して経済的許容限界に達した場合のみ、緩衝帯を従来の殺有害生物剤で処理してよい
− Btベースの噴霧可能な殺虫剤は、緩衝帯トウモロコシに用いることができない
− Btトウモロコシの農場毎に適切な緩衝帯を設置しなければならない」
(参考文献)
Wolfersberger, M.G., (1993), Preparation and Partial Characterization of Amino Acid Transporting Brush Border Membrane Vesicles from the Larval Midgut of the Gypsy Moth (Lymantria Dispar). Arch. Insect Biochem. Physiol. 24: 139-147.
Liang, Y., Patel, S.S., and Dean, D.H., (1995), Irreversible Binding Kinetics of Bacillus thuringiensis Cry1A Delta-Endotoxins to Gypsy Moth Brush Border Membrane Vesicles is Directly Correlated to Toxicity. J. Bioi. Chern., 270, 24719-24724
バイオアッセイ
Cry1Faに対し感受性および抵抗性(rFAWおよびrECB)両方の他の主要な害虫に対するその活性に関し、対象Cryタンパク質を試験した。表1に示す通り、Cry1IおよびCry2Aaは、抵抗性および感受性両方のECB幼虫に対し活性を有する。Cry1Eaは、抵抗性および感受性両方のFAWに対し活性を有する(この有害生物の概略的な考察に関し、例えば、Tabashnik、PNAS (2008)、105巻49号、19029〜19030を参照されたし)。
本願発明は以下の態様に関するものである。
(1) Cry1Fa殺虫性タンパク質をコードするDNAと、Cry2Aa殺虫性タンパク質およびCry1I殺虫性タンパク質からなる群から選択される第二の殺虫性タンパク質をコードするDNAとを含むトランスジェニック植物。
(2) Cry1Fa殺虫性タンパク質をコードするDNAと、Cry1E殺虫性タンパク質をコードするDNAとを含むトランスジェニック植物。
(3) Cry1Be、Cry1AbおよびDIG−3からなる群から選択される第三の殺虫性タンパク質をコードするDNAをさらに含む、(1)に記載のトランスジェニック植物。
(4) 前記第三のタンパク質が、Cry1Beからなる群から選択され、前記植物が、Cry1Ca、Cry1DaおよびVip3Abからなる群から選択される第四の殺虫性タンパク質をコードするDNAをさらに含む、(3)に記載のトランスジェニック植物。
(5) Cry1Be、Cry1Ca、Cry1DaおよびVip3Abからなる群から選択される第三の殺虫性タンパク質をコードするDNAをさらに含む、(2)に記載のトランスジェニック植物。
(6) 前記第三のタンパク質が、Cry1Beからなる群から選択され、前記植物が、Cry1Ab、Cry1I、Cry2AおよびDIG−3からなる群から選択される第四の殺虫性タンパク質をコードするDNAをさらに含む、(5)に記載のトランスジェニック植物。
(7) 前記DNAを含む、(1)〜(6)のいずれかに記載の植物の種子。
(8) 非Bt緩衝帯植物および(1)〜(6)のいずれかに記載の複数の植物を含む植物の圃場であって、前記緩衝帯植物は前記圃場の全ての作物植物の40%未満を構成する、圃場。
(9) 前記緩衝帯植物が前記圃場の全ての作物植物の30%未満を構成する、(8)に記載の植物の圃場。
(10) 前記緩衝帯植物が前記圃場の全ての作物植物の20%未満を構成する、(8)に記載の植物の圃場。
(11) 前記緩衝帯植物が前記圃場の全ての作物植物の10%未満を構成する、(8)に記載の植物の圃場。
(12) 前記緩衝帯植物が前記圃場の全ての作物植物の5%未満を構成する、(8)に記載の植物の圃場。
(13) 前記緩衝帯植物がブロックまたは帯状地にある、(8)に記載の植物の圃場。
(14) 非Bt緩衝帯植物からの緩衝帯種子および(7)に記載の複数の種子を含む種子混合物であって、前記緩衝帯種子は混合物の全ての種子の40%未満を構成する、種子混合物。
(15) 前記緩衝帯種子が混合物の全ての種子の30%未満を構成する、(14)に記載の種子混合物。
(16) 前記緩衝帯種子が混合物の全ての種子の20%未満を構成する、(14)に記載の種子混合物。
(17) 前記緩衝帯種子が混合物の全ての種子の10%未満を構成する、(14)に記載の種子混合物。
(18) 前記緩衝帯種子が混合物の全ての種子の5%未満を構成する、(14)に記載の種子混合物。
(19) 種子を播いて(8)に記載の植物の圃場を作製するステップを含む、昆虫によるCry毒素への抵抗性の発達を管理する方法。
(20) 前記植物が10エーカーよりも多くを占める、(8)〜(13)のいずれかに記載の圃場。
(21) 前記植物がトウモロコシ、ダイズおよびワタからなる群から選択される、(1)〜(6)のいずれかに記載の植物。
(22) 前記植物がトウモロコシ植物である、(21)に記載の植物。
(23) ユーロピアンコーンボーラー昆虫をCry1F殺虫性タンパク質ならびにCry2Aa殺虫性タンパク質およびCry1I殺虫性タンパク質からなる群から選択される第二の殺虫性タンパク質と接触させることにより、ユーロピアンコーンボーラー昆虫を防除する方法。
(24) Cry2Aa殺虫性タンパク質およびCry1I殺虫性タンパク質からなる群から選択される第二の殺虫性タンパク質と組み合わせたCry1F殺虫性タンパク質をコードする単離されたポリヌクレオチド。
(25) Cry2Aa殺虫性タンパク質およびCry1I殺虫性タンパク質からなる群から選択される第二の殺虫性タンパク質と組み合わせたCry1F殺虫性タンパク質を作製する方法。
(26) ツマジロクサヨトウ昆虫をCry1F殺虫性タンパク質およびCry1E殺虫性タンパク質と接触させることにより、ツマジロクサヨトウ昆虫を防除する方法。
(27) Cry1E殺虫性タンパク質と組み合わせたCry1F殺虫性タンパク質をコードする単離されたポリヌクレオチド。
(28) Cry1E殺虫性タンパク質と組み合わせたCry1F殺虫性タンパク質を作製する方法。
(29) (1)に記載の植物の植物細胞であって、前記Cry1Fa殺虫性タンパク質が、配列番号1と少なくとも99%同一であり、前記Cry2Aa殺虫性タンパク質が、配列番号2と少なくとも99%同一であり、前記Cry1I殺虫性タンパク質が、配列番号3と少なくとも99%同一である、植物細胞。
(30) (2)に記載の植物の植物細胞であって、前記Cry1Fa殺虫性タンパク質が、配列番号1と少なくとも99%同一であり、前記Cry1E殺虫性タンパク質が、配列番号4と少なくとも99%同一である、植物細胞。
Claims (2)
- ユーロピアンコーンボーラー昆虫をCry1F殺虫性タンパク質ならびにCry2Aa殺虫性タンパク質およびCry1I殺虫性タンパク質からなる群から選択される第二の殺虫性タンパク質と接触させることにより、ユーロピアンコーンボーラー昆虫を防除する方法。
- ツマジロクサヨトウ昆虫をCry1F殺虫性タンパク質およびCry1E殺虫性タンパク質と接触させることにより、ツマジロクサヨトウ昆虫を防除する方法。
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US28427809P | 2009-12-16 | 2009-12-16 | |
US61/284,278 | 2009-12-16 | ||
PCT/US2010/060812 WO2011075586A1 (en) | 2009-12-16 | 2010-12-16 | Insecticidal protein combinations for controlling fall armyworm and european corn borer, and methods for insect resistance managements |
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JP2012544847A Pending JP2013514776A (ja) | 2009-12-16 | 2010-12-16 | ユーロピアンコーンボーラー防除のためのCRY1AbおよびCRY2Aaを含む殺虫性タンパク質の組み合わせならびに昆虫抵抗性管理のための方法 |
JP2012544837A Expired - Fee Related JP5907891B2 (ja) | 2009-12-16 | 2010-12-16 | ツマジロクサヨトウ(fallarmyworm)およびユーロピアンコーンボーラー(Europeancornborer)を防除するための殺虫性タンパク質の組み合わせならびに害虫抵抗性管理のための方法 |
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EP (3) | EP2512220B1 (ja) |
JP (2) | JP2013514776A (ja) |
KR (2) | KR20120115316A (ja) |
CN (3) | CN107177627A (ja) |
AR (2) | AR079622A1 (ja) |
AU (2) | AU2010330915B2 (ja) |
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CA (2) | CA2782627A1 (ja) |
CL (3) | CL2012001634A1 (ja) |
CO (2) | CO6561808A2 (ja) |
IL (2) | IL220336A (ja) |
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NZ (4) | NZ630817A (ja) |
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Families Citing this family (65)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103533828A (zh) * | 2010-12-16 | 2014-01-22 | 陶氏益农公司 | Vip3Ab和Cry1Ab用于管理抗性昆虫的组合用途 |
BR102012019434B1 (pt) | 2011-07-26 | 2021-11-09 | Dow Agrosciences Llc | Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1 |
CN103841821A (zh) * | 2011-08-05 | 2014-06-04 | 陶氏益农公司 | 与Cry1Ab组合的DIG3杀虫晶体蛋白的用途 |
US10119149B2 (en) | 2011-08-05 | 2018-11-06 | Dow Agrosciences Llc | Use of DIG3 insecticidal crystal protein in combination with cry1Ab for management of resistance in european cornborer |
CA2886787A1 (en) * | 2012-10-05 | 2014-04-10 | Dow Agrosciences Llc | Use of cry1ea in combinations for management of resistant fall armyworm insects |
WO2014071182A1 (en) | 2012-11-01 | 2014-05-08 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
CN102972428B (zh) * | 2012-12-11 | 2014-07-09 | 北京大北农科技集团股份有限公司 | 控制害虫的方法 |
US10023877B2 (en) | 2013-03-15 | 2018-07-17 | Pioneer Hi-Bred International, Inc. | PHI-4 polypeptides and methods for their use |
EP3032942B1 (en) | 2013-08-16 | 2020-03-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3043635B1 (en) | 2013-09-13 | 2020-02-12 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN103688974B (zh) * | 2013-11-11 | 2015-07-15 | 北京大北农科技集团股份有限公司 | 控制害虫的方法 |
CN103757049B (zh) * | 2013-12-24 | 2016-06-08 | 北京大北农科技集团股份有限公司 | 控制害虫的构建体及其方法 |
CN103725704B (zh) * | 2014-01-17 | 2015-11-18 | 北京大北农科技集团股份有限公司 | 控制害虫的构建体及其方法 |
WO2015120276A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
EP3102684B1 (en) | 2014-02-07 | 2020-05-06 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2016000237A1 (en) | 2014-07-03 | 2016-01-07 | Pioneer Overseas Corporation | Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes |
US10435706B2 (en) | 2014-10-16 | 2019-10-08 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EA038923B1 (ru) | 2015-03-11 | 2021-11-10 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидная днк-конструкция и способы её применения |
RU2017144238A (ru) | 2015-05-19 | 2019-06-19 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
AU2016278142A1 (en) | 2015-06-16 | 2017-11-30 | E. I. Du Pont De Nemours And Company | Compositions and methods to control insect pests |
AR104833A1 (es) | 2015-07-01 | 2017-08-16 | Syngenta Participations Ag | Composiciones y métodos para controlar plagas de plantas |
AR105155A1 (es) | 2015-07-07 | 2017-09-13 | Syngenta Participations Ag | Composiciones y métodos para controlar plagas de plantas |
MX2018000615A (es) | 2015-07-13 | 2018-08-01 | Pivot Bio Inc | Metodos y composiciones para mejorar atributos de plantas. |
MX2018001523A (es) | 2015-08-06 | 2018-03-15 | Pioneer Hi Bred Int | Proteinas insecticidas derivadas de plantas y metodos para su uso. |
EP3341483B1 (en) | 2015-08-28 | 2019-12-18 | Pioneer Hi-Bred International, Inc. | Ochrobactrum-mediated transformation of plants |
WO2017062412A1 (en) | 2015-10-05 | 2017-04-13 | Massachusetts Institute Of Technology | Nitrogen fixation using refactored nif clusters |
US20180325119A1 (en) | 2015-12-18 | 2018-11-15 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN117003840A (zh) | 2016-04-14 | 2023-11-07 | 先锋国际良种公司 | 具有改善的活性谱的杀昆虫多肽及其用途 |
US11028407B2 (en) | 2016-04-19 | 2021-06-08 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof |
EP3445160A4 (en) * | 2016-04-19 | 2019-10-30 | Dow Agrosciences LLC | COMBINATION OF FOUR VIP AND CRY PROTEIN TOXINS FOR CONTROL OF INSECT PESTS IN PLANTS |
EP3960863A1 (en) | 2016-05-04 | 2022-03-02 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US20190185867A1 (en) | 2016-06-16 | 2019-06-20 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
US20190194676A1 (en) | 2016-06-24 | 2019-06-27 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
EP3954202A1 (en) | 2016-07-01 | 2022-02-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
UA127343C2 (uk) | 2016-07-29 | 2023-07-26 | Монсанто Текнолоджі Ллс | Спосіб і композиція для експресії генів в рослині |
EP4050021A1 (en) | 2016-11-01 | 2022-08-31 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN106749566B (zh) * | 2016-11-21 | 2020-05-05 | 北京大北农科技集团股份有限公司 | 杀虫蛋白组合及其管理昆虫抗性的方法 |
CN110088123B (zh) | 2016-12-14 | 2023-10-20 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
CA3046226A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN110267976B (zh) | 2017-01-12 | 2024-01-26 | 孟山都技术公司 | 对鳞翅目昆虫具有活性的杀害虫毒素蛋白质 |
WO2018132325A1 (en) * | 2017-01-12 | 2018-07-19 | Syngenta Participations Ag | Insecticidal proteins |
CN110799474B (zh) | 2017-01-12 | 2022-07-26 | 皮沃特生物公司 | 用于改良植物性状的方法及组合物 |
WO2018140214A1 (en) | 2017-01-24 | 2018-08-02 | Pioneer Hi-Bred International, Inc. | Nematicidal protein from pseudomonas |
WO2018148001A1 (en) | 2017-02-08 | 2018-08-16 | Pioneer Hi-Bred International Inc | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
EP3622076A1 (en) | 2017-05-11 | 2020-03-18 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
BR112019024827A2 (pt) | 2017-05-26 | 2020-06-16 | Pioneer Hi-Bred International, Inc. | Construto de dna, planta transgênica ou progênie da mesma, composição e método para controlar uma população de pragas de insetos |
CN107474120B (zh) * | 2017-08-16 | 2020-08-18 | 中国农业大学 | 人工合成用于转基因抗虫植物的Bt杀虫基因mcry1F |
US20200165626A1 (en) | 2017-10-13 | 2020-05-28 | Pioneer Hi-Bred International, Inc. | Virus-induced gene silencing technology for insect control in maize |
US11993778B2 (en) | 2017-10-25 | 2024-05-28 | Pivot Bio, Inc. | Methods and compositions for improving engineered microbes that fix nitrogen |
EP3755797A1 (en) | 2018-02-22 | 2020-12-30 | Zymergen, Inc. | Method for creating a genomic library enriched for bacillus and identification of novel cry toxins |
WO2019169227A1 (en) | 2018-03-02 | 2019-09-06 | Zymergen Inc. | Insecticidal protein discovery platform and insecticidal proteins discovered therefrom |
US20210002657A1 (en) | 2018-03-02 | 2021-01-07 | Pioneer Hi-Bred International, Inc. | Plant health assay |
CA3096516A1 (en) | 2018-05-22 | 2019-11-28 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
US11963530B2 (en) | 2018-06-27 | 2024-04-23 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
EP3844283A1 (en) | 2018-08-29 | 2021-07-07 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
WO2021221690A1 (en) | 2020-05-01 | 2021-11-04 | Pivot Bio, Inc. | Modified bacterial strains for improved fixation of nitrogen |
US11580389B2 (en) | 2020-01-14 | 2023-02-14 | International Business Machines Corporation | System and method for predicting fall armyworm using weather and spatial dynamics |
CA3172322A1 (en) | 2020-05-01 | 2021-11-04 | Karsten TEMME | Modified bacterial strains for improved fixation of nitrogen |
US20230235352A1 (en) | 2020-07-14 | 2023-07-27 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP4192848A1 (en) | 2020-08-10 | 2023-06-14 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
WO2023278804A1 (en) | 2021-07-02 | 2023-01-05 | Pivot Bio, Inc. | Genetically-engineered bacterial strains for improved fixation of nitrogen |
CN114128728B (zh) * | 2021-12-17 | 2023-03-21 | 湖北省农业科学院植保土肥研究所 | 去氢木香内酯对草地贪夜蛾的生物活性及其在农业防治中的应用 |
WO2023156906A1 (en) * | 2022-02-15 | 2023-08-24 | Futuragene Israel Ltd | A bacillus thuringiensis pesticidal protein (bt pp) combination useful for plant protection |
Family Cites Families (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5338544A (en) * | 1987-04-16 | 1994-08-16 | Ecogen Inc. | CryIIB protein, insecticidal compositions and methods of use thereof |
US5045469A (en) * | 1988-10-27 | 1991-09-03 | Mycogen Corporation | Novel bacillus thuringiensis isolate denoted B. T. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin |
US5188960A (en) | 1989-06-27 | 1993-02-23 | Mycogen Corporation | Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins |
US6329574B1 (en) * | 1990-01-22 | 2001-12-11 | Dekalb Genetics Corporation | High lysine fertile transgenic corn plants |
US5273746A (en) * | 1992-01-29 | 1993-12-28 | Mycogen Corporation | Bacillus thuringiensis isolates active against phthiraptera pests |
EP0628051B1 (en) | 1992-02-12 | 2003-07-02 | Chromagen, Inc. | Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides |
GB9318207D0 (en) * | 1993-09-02 | 1993-10-20 | Sandoz Ltd | Improvements in or relating to organic compounds |
US5527883A (en) * | 1994-05-06 | 1996-06-18 | Mycogen Corporation | Delta-endotoxin expression in pseudomonas fluorescens |
US5508264A (en) | 1994-12-06 | 1996-04-16 | Mycogen Corporation | Pesticidal compositions |
ID25530A (id) * | 1996-11-20 | 2000-10-12 | Ecogen Inc | δ-ENDOTOKSIN BERSPEKTRUM-LEBAR |
US6218188B1 (en) * | 1997-11-12 | 2001-04-17 | Mycogen Corporation | Plant-optimized genes encoding pesticidal toxins |
CA2325567A1 (en) * | 1998-05-01 | 1999-11-11 | Maxygen, Inc. | Optimization of pest resistance genes using dna shuffling |
US6489542B1 (en) * | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
AR024430A1 (es) * | 1999-06-29 | 2002-10-02 | Syngenta Ltd | Proteinas insecticidas y combinaciones sinergicas de las mismas |
CA2384967A1 (en) * | 1999-09-15 | 2001-03-22 | Monsanto Technology Llc | Lepidopteran-active bacillus thuringiensis .delta.-endotoxin compositions and methods of use |
US6750379B2 (en) * | 2000-03-09 | 2004-06-15 | Dekalb Genetics Corporation | Homologous recombination-mediated transgene alterations in plants |
US6551962B1 (en) | 2000-10-06 | 2003-04-22 | Monsanto Technology Llc | Method for deploying a transgenic refuge |
BRPI0206346B1 (pt) * | 2001-01-09 | 2018-04-03 | Bayer Cropscience Nv | Dna que codifica uma proteína inseticida cry2ae, gene quimérico compreendendo o mesmo, proteína inseticida, uso da mesma, micro-organismo, bem como processo para tornar uma planta resistente a um inseto lepidóptero |
US7053266B2 (en) * | 2002-03-27 | 2006-05-30 | Council Of Scientfic And Industrial Research | Chimeric cry1E δendotoxin and methods of controlling insects |
US7351881B2 (en) * | 2003-02-20 | 2008-04-01 | Athenix Corporation | AXMI-008, a delta-endotoxin gene and methods for its use |
US7355099B2 (en) * | 2003-02-20 | 2008-04-08 | Athenix Corporation | AXMI-004, a delta-endotoxin gene and methods for its use |
NZ588825A (en) * | 2003-02-20 | 2011-06-30 | Athenix Corp | AXMI-014 delta-endotoxin |
US7253343B2 (en) * | 2003-08-28 | 2007-08-07 | Athenix Corporation | AXMI-003, a delta-endotoxin gene and methods for its use |
AR048747A1 (es) * | 2004-03-05 | 2006-05-24 | Agrigenetics Inc | Combinaciones de cry1ab y cry1fa como una herramienta para el control de la resistencia de los insectos |
US7179965B2 (en) * | 2004-03-26 | 2007-02-20 | Dow Agrosciences Llc | Cry1F and Cry1Ac transgenic cotton lines and event-specific identification thereof |
US20080226753A1 (en) * | 2004-03-29 | 2008-09-18 | Pioneer Hi-Bred International, Inc. | Method of Reducing Insect Resistant Pests in Transgenic Crops |
WO2008085729A2 (en) * | 2006-12-22 | 2008-07-17 | Pioneer Hi-Bred International, Inc. | Resistance management strategies for transgenic crops |
US20080256669A1 (en) * | 2007-04-16 | 2008-10-16 | Monsanto Company | Plants with Multiple Transgenes on a Chromosome |
CN101688216B (zh) * | 2007-06-01 | 2014-03-26 | 拜尔作物科学公司 | 编码杀虫蛋白质的新基因 |
WO2009132850A1 (en) * | 2008-05-01 | 2009-11-05 | Bayer Bioscience N.V. | Armyworm insect resistance management in transgenic plants |
US20110088129A1 (en) * | 2008-06-13 | 2011-04-14 | Carmen Sara Hernandez | Bollworm Insect Resistance Management in Transgenic Plants |
US20090313717A1 (en) * | 2008-06-16 | 2009-12-17 | Carmen Sara Hernandez | Bollworm insect resistance management in transgenic plants |
CN102066566A (zh) * | 2008-06-13 | 2011-05-18 | 拜尔生物科学公司 | 转基因植物中的棉铃虫昆虫抗性管理 |
AU2010236944B2 (en) | 2009-04-17 | 2016-07-21 | Dow Agrosciences Llc | DIG-3 insecticidal Cry toxins |
WO2011050271A1 (en) * | 2009-10-23 | 2011-04-28 | Monsanto Technology Llc | Methods and compositions for expression of transgenes in plants |
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