JP5711968B2 - 高エイコサペンタエン酸を生成するためのヤロウィア・リポリティカ(YarrowiaLipolytica)の最適化株 - Google Patents

高エイコサペンタエン酸を生成するためのヤロウィア・リポリティカ(YarrowiaLipolytica)の最適化株 Download PDF

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JP5711968B2
JP5711968B2 JP2010528141A JP2010528141A JP5711968B2 JP 5711968 B2 JP5711968 B2 JP 5711968B2 JP 2010528141 A JP2010528141 A JP 2010528141A JP 2010528141 A JP2010528141 A JP 2010528141A JP 5711968 B2 JP5711968 B2 JP 5711968B2
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seq
gene
desaturase
epa
yarrowia lipolytica
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JP2011501661A (ja
JP2011501661A5 (enExample
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チーシオン・シュエ
ナレンドラ・エス・ヤダヴ
クイン・クン・ズュー
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EIDP Inc
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JP2010528141A 2007-10-03 2008-10-03 高エイコサペンタエン酸を生成するためのヤロウィア・リポリティカ(YarrowiaLipolytica)の最適化株 Expired - Fee Related JP5711968B2 (ja)

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Application Number Priority Date Filing Date Title
US97717407P 2007-10-03 2007-10-03
US97717707P 2007-10-03 2007-10-03
US60/977,174 2007-10-03
US60/977,177 2007-10-03
PCT/US2008/078642 WO2009046231A1 (en) 2007-10-03 2008-10-03 Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

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JP2011501661A JP2011501661A (ja) 2011-01-13
JP2011501661A5 JP2011501661A5 (enExample) 2011-11-17
JP5711968B2 true JP5711968B2 (ja) 2015-05-07

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EP (1) EP2195415A1 (enExample)
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CA (1) CA2701094A1 (enExample)
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Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8313911B2 (en) * 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US8119860B2 (en) * 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7943365B2 (en) * 2007-05-03 2011-05-17 E.I. Du Pont De Nemours And Company Δ-5 desaturases and their use in making polyunsaturated fatty acids
JP2010528627A (ja) 2007-06-01 2010-08-26 ソラザイム、インク 微生物による油の生産
EP2324120B1 (en) 2008-08-29 2016-01-27 E. I. du Pont de Nemours and Company Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms
DK2324119T3 (en) * 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
CA2744716C (en) 2008-12-18 2017-05-23 E.I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process
US8207363B2 (en) * 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
CN102803289B (zh) * 2009-06-16 2015-07-22 纳幕尔杜邦公司 用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株
US8399226B2 (en) * 2009-06-16 2013-03-19 E I Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica
WO2010147907A1 (en) 2009-06-16 2010-12-23 E. I. Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica
BRPI1009690A2 (pt) * 2009-06-16 2015-08-25 Du Pont "célula hospedeira microbiana oleaginosa, óleo, método de elaboração de um óleo, método de aumento da eficiência de conversão de alongamento de c18 para c20 em uma célula hospedeira recombinante microbiana oleaginosa e método de aumento da eficiência de conversão de dessaturação (delta) 4 em uma célula hospedeira recombinante microbiana oleaginosa produtora de ácidos graxos póli-insaturados de cadeia longa"
WO2011087981A2 (en) 2010-01-15 2011-07-21 E. I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
CA2993690C (en) 2010-01-19 2022-03-22 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
CN103282474A (zh) 2010-04-22 2013-09-04 纳幕尔杜邦公司 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法
SG10201504197SA (en) * 2010-05-28 2015-06-29 Solazyme Inc Food Compositions Comprising Tailored Oils
JP5678180B2 (ja) 2010-06-09 2015-02-25 フォトンズ コーポレーション リミテッド 高度精製に適した、エイコサペンタエン酸を含む組成物
WO2012021686A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Improved aquaculture meat products
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
TW201207116A (en) 2010-08-13 2012-02-16 Univ Fu Jen Catholic A method for producing oil by Cystofilobasidium spp.
WO2012027676A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
US8703473B2 (en) 2010-08-26 2014-04-22 E I Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
AU2011293189B2 (en) * 2010-08-26 2017-02-16 E. I. Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
EP3521408B1 (en) 2010-11-03 2021-12-22 Corbion Biotech, Inc. Genetically-engineered chlorella or prototheca microbe and oil produced therefrom
CN103502461A (zh) 2010-12-30 2014-01-08 纳幕尔杜邦公司 在含油酵母中通过提高yap1转录因子活性提高油含量
BR112013016769A2 (pt) 2010-12-30 2016-10-11 Du Pont yarrowia lipolytica transformada e método para produzir pelo menos um produto não nativo de interesse
CN110066836A (zh) 2011-02-02 2019-07-30 柯碧恩生物技术公司 产自重组产油微生物的定制油
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
JP6061866B2 (ja) * 2011-02-11 2017-01-18 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company 微生物バイオマスから脂質含有組成物を得る方法
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8969049B2 (en) 2011-03-31 2015-03-03 E I Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
EP2694665A1 (en) 2011-04-01 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
EP2702148A1 (en) 2011-04-07 2014-03-05 E.I. Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast
EP2714724A1 (en) 2011-05-26 2014-04-09 E. I. Du Pont de Nemours and Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
US8544772B2 (en) * 2011-05-27 2013-10-01 Ginn Mineral Technology, Inc. Method for the recovery of minerals
CN105255738B (zh) * 2011-07-21 2024-12-27 帝斯曼知识产权资产管理有限公司 生产二十碳五烯酸的微生物、脂肪酸组合物及其制作方法与用途
EA035287B1 (ru) * 2011-07-21 2020-05-25 ДСМ АйПи АССЕТС Б.В. Микроорганизмы, продуцирующие эйкозапентаеновую кислоту, композиции, содержащие жирные кислоты, и способы их получения и применения
ES2857173T3 (es) * 2011-07-21 2021-09-28 Dsm Ip Assets Bv Aceites microbianos enriquecidos en ácidos grasos poliinsaturados
CN103031349B (zh) * 2011-09-29 2014-07-09 私立辅仁大学 利用Cystofilobasidium属的酵母菌产生油脂的方法和系统
BR112014025719A8 (pt) 2012-04-18 2017-10-03 Solazyme Inc Óleos customizados
WO2013192007A1 (en) 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES
CN102787128A (zh) * 2012-08-28 2012-11-21 江南大学 一种用于多不饱和脂肪酸生物合成的脱饱和酶Δ9 Des
US9701989B2 (en) 2012-12-21 2017-07-11 E I Du Pont De Nemours And Company Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight
CA2892516A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
US10123986B2 (en) 2012-12-24 2018-11-13 Qualitas Health, Ltd. Eicosapentaenoic acid (EPA) formulations
MX372724B (es) 2012-12-24 2020-06-19 Qualitas Health Inc Formulaciones de acido eicosapentaenoico (epa).
US9629820B2 (en) 2012-12-24 2017-04-25 Qualitas Health, Ltd. Eicosapentaenoic acid (EPA) formulations
CN105431529A (zh) 2013-05-03 2016-03-23 德克萨斯大学系统董事会 用于真菌脂质生产的组合物和方法
MX369685B (es) 2013-10-04 2019-11-19 Terravia Holdings Inc Aceites adaptables.
CN103749744A (zh) * 2014-02-24 2014-04-30 四川省三台县通宇食品有限公司 一种富含氨基酸食用菌油的制备方法
EP3620517A3 (en) 2014-07-10 2020-07-29 Corbion Biotech, Inc. Ketoacyl acp synthase genes and uses thereof
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
IL241841B (en) * 2014-09-30 2019-09-26 Univ Free State Production of proteins for human and animal health using yarrowia lipolytica
EP3626832B1 (en) * 2014-11-25 2024-06-12 The Brigham and Women's Hospital, Inc. Method of identifying a person having a predisposition to or afflicted with a cardiometabolic disease
WO2016085876A1 (en) 2014-11-25 2016-06-02 The Broad Institute Inc. Clonal haematopoiesis
US9682034B2 (en) 2015-03-10 2017-06-20 Elc Management Llc Methods and compositions for treating skin to resolve inflammation and screening for actives that stimulate pro-resolution pathways
CN104805027B (zh) * 2015-05-27 2018-04-27 天津大学 一种重组耶氏解脂酵母菌株及其构建方法和应用
JP6898915B2 (ja) 2015-07-22 2021-07-07 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company 生物を用いる長鎖ジカルボン酸の高レベルの生産
GB201602410D0 (en) 2016-02-10 2016-03-23 Tate & Lyle Ingredients Methods for improving the gastrointestinal tolerance of food and beverage products comprising sweet, low-digestible carbohydrates
JP6897917B2 (ja) 2015-10-01 2021-07-07 ディーエスエム アイピー アセッツ ビー.ブイ.Dsm Ip Assets B.V. ペットフードに用いるサプリメント素材
FI4144844T3 (fi) 2015-10-12 2025-11-24 Dupont Us Holding Llc Suojatut dna-templaatit geenimuokkaukseen ja homologisen rekombinaation lisäämiseen soluissa ja niiden käyttömenetelmät
CN106190872B (zh) * 2016-09-23 2019-10-25 厦门大学 发酵罐底物流加生产二十二碳六烯酸的方法
EP4159850A1 (en) 2016-12-16 2023-04-05 Danmarks Tekniske Universitet Production of desaturated fatty alcohols and desaturated fatty alcohol acetates in yeast
WO2018109163A1 (en) 2016-12-16 2018-06-21 Danmarks Tekniske Universitet Methods for producing fatty alcohols and derivatives thereof in yeast
EP3348647A1 (en) 2017-01-13 2018-07-18 Institut National De La Recherche Agronomique Mutant yeast strain capable of producing medium chain fatty acids
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
CN107502601B (zh) * 2017-06-13 2020-04-21 天津科技大学 一种胰蛋白酶抗性改良的脂肪酶突变体及其基因和应用
WO2019143660A1 (en) * 2018-01-16 2019-07-25 Trustees Of Boston University Clonal hematopoiesis and cytokine targets
US11913046B2 (en) 2018-04-23 2024-02-27 Inbiose N.V. Increasing export of 2'fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
MX2021008018A (es) 2019-01-03 2021-12-10 Corbion Biotech Inc Procedimiento para la fabricacion de suspension de celulas lisadas.
CN110499259B (zh) * 2019-07-22 2021-07-27 浙江工业大学 一种解酯耶氏酵母yw100-1及其应用
US11572546B2 (en) 2019-07-29 2023-02-07 Ginkgo Bioworks, Inc. Methods and compositions involving promoters derived from Yarrowia lipolytica
WO2022235655A1 (en) 2021-05-04 2022-11-10 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
US20240239921A1 (en) 2021-05-04 2024-07-18 Nutrition & Biosciences USA 4, Inc. Compositions comprising oxidized insoluble alpha-glucan
EP4463519A1 (en) 2022-01-12 2024-11-20 Nutrition & Biosciences USA 4, Inc. Coating compositions comprising rubber and insoluble alpha-glucan
CA3246295A1 (en) 2022-03-21 2023-09-28 Nutrition & Biosciences Usa 4 Inc COMPOSITIONS CONTAINING AN INSOLUBLE ALPHA-GLUCANE
JP2025539450A (ja) * 2022-12-02 2025-12-05 マラ リニューアブルズ コーポレーション 操作された脂質特性を有する油組成物及びその生成方法
CN116121093B (zh) * 2022-12-12 2025-11-07 浙江大学杭州国际科创中心 一种用于表达细胞色素p450的工程酵母菌株及其应用
WO2025207802A1 (en) 2024-03-27 2025-10-02 Nutrition & Biosciences USA 4, Inc. Recombinant microbial strains comprising reduced beta-oxidation phenotypes and methods thereof
CN118703533B (zh) * 2024-08-01 2025-11-21 湖南农业大学 OsGAT3基因在调控水稻Cd、Cu或Mn含量及株高中的应用

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2052564T3 (es) 1986-07-08 1994-07-16 Suntory Ltd Procedimiento para la produccion de acido bishomo-gamma-linolenico y acido eicosapanteonico.
US5246841A (en) 1986-12-26 1993-09-21 Sagami Chemical Research Center Microbial process for production of eicosapentaenoic acid
US5244921A (en) 1990-03-21 1993-09-14 Martek Corporation Eicosapentaenoic acids and methods for their production
US5246842A (en) 1991-10-30 1993-09-21 The United States Of America As Represented By The Secretary Of Agriculture Production of eicosapentaenoic acid from filamentous fungi utilizing lactose as a primary carbon source
DK0956013T3 (da) 1996-10-11 2003-08-04 Scarista Ltd Farmaceutisk præparat indeholdende eicosapentaensyre og/eller stearidonsyre
US5968809A (en) 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6825017B1 (en) 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
EP1088552B1 (en) * 1999-09-30 2006-04-19 Loders Croklaan B.V. Compositions containing pinolenic acid and its use a health component
EP1279400A1 (en) 2001-07-25 2003-01-29 Target Hit sa Modifying the fatty acid composition of cell membranes of organs and tissues
US7410663B2 (en) 2002-08-07 2008-08-12 Kao Corporation Oil or fat composition
WO2004018598A1 (ja) 2002-08-07 2004-03-04 Kao Corporation 油脂組成物
AU2003301007B2 (en) 2002-12-20 2008-01-31 The Procter & Gamble Company Tufted laminate web
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US8313911B2 (en) * 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7214491B2 (en) 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7504259B2 (en) * 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
US7550651B2 (en) 2004-06-25 2009-06-23 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7465564B2 (en) 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US8048652B2 (en) * 2005-05-12 2011-11-01 Martek Biosciences Corporation Biomass hydrolysate and uses and production thereof
FR2888976B1 (fr) 2005-07-25 2007-10-12 Oberthur Card Syst Sa Entite electronique avec moyens de communication par contact et a distance
WO2007061742A1 (en) 2005-11-23 2007-05-31 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US20070225370A1 (en) 2006-03-21 2007-09-27 Joar Opheim Nutritional Compositions and Methods
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
DK2129777T3 (en) 2007-04-03 2016-01-25 Du Pont MULTIZYMER AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
US8188338B2 (en) * 2007-04-10 2012-05-29 E. I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids

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