CA2701094A1 - Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production - Google Patents

Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production Download PDF

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Publication number
CA2701094A1
CA2701094A1 CA2701094A CA2701094A CA2701094A1 CA 2701094 A1 CA2701094 A1 CA 2701094A1 CA 2701094 A CA2701094 A CA 2701094A CA 2701094 A CA2701094 A CA 2701094A CA 2701094 A1 CA2701094 A1 CA 2701094A1
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Prior art keywords
acid
oil
weight percent
desaturase
seq
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Abandoned
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CA2701094A
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English (en)
French (fr)
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Zhixiong Xue
Narendra S. Yadav
Quinn Qun Zhu
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EIDP Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
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  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Diabetes (AREA)
  • Molecular Biology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Rheumatology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Psychiatry (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Mycology (AREA)
  • Pain & Pain Management (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
CA2701094A 2007-10-03 2008-10-03 Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production Abandoned CA2701094A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US97717407P 2007-10-03 2007-10-03
US97717707P 2007-10-03 2007-10-03
US60/977,174 2007-10-03
US60/977,177 2007-10-03
PCT/US2008/078642 WO2009046231A1 (en) 2007-10-03 2008-10-03 Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

Publications (1)

Publication Number Publication Date
CA2701094A1 true CA2701094A1 (en) 2009-04-09

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ID=40084411

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Application Number Title Priority Date Filing Date
CA2701094A Abandoned CA2701094A1 (en) 2007-10-03 2008-10-03 Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

Country Status (6)

Country Link
US (5) US8323935B2 (enExample)
EP (1) EP2195415A1 (enExample)
JP (1) JP5711968B2 (enExample)
CN (1) CN101970638B (enExample)
CA (1) CA2701094A1 (enExample)
WO (1) WO2009046231A1 (enExample)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8313911B2 (en) * 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7794701B2 (en) * 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US8957280B2 (en) * 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
CN104212844A (zh) 2007-06-01 2014-12-17 索拉兹米公司 在微生物中生产油
JP5656836B2 (ja) 2008-08-29 2015-01-21 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 油性生物中の含油量の変更のためのｓｎｆ１タンパク質キナーゼ活性の操作
EP2324119B1 (en) * 2008-09-19 2016-03-30 E. I. du Pont de Nemours and Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
WO2010080388A1 (en) 2008-12-18 2010-07-15 E. I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
AU2010260227B2 (en) 2009-06-16 2015-01-29 E. I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases
US8399226B2 (en) * 2009-06-16 2013-03-19 E I Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica
DK2442666T3 (da) * 2009-06-16 2015-07-13 Du Pont Olier med højt indhold af eicosapentaensyre fra forbedrede og optimerede stammer af yarrowia lipolytica
CA2761589C (en) * 2009-06-16 2017-12-19 E. I. Du Pont De Nemours And Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
US20110178105A1 (en) * 2010-01-15 2011-07-21 E.I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
BR122015020119B8 (pt) 2010-01-19 2021-05-04 Dsm Ip Assets Bv óleo microbiano, produtos alimentícios para um animal não humano e biomassa isolada
EP2561050A1 (en) 2010-04-22 2013-02-27 E.I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
EP2576800B1 (en) * 2010-05-28 2019-01-30 Corbion Biotech, Inc. Method for producing oils from Prototheca
US8769868B2 (en) 2010-06-09 2014-07-08 Photonz Corporation Limited Compositions comprising eicosapentaenoic acid suitable for high purification
US20120207912A1 (en) 2010-08-11 2012-08-16 E.I. Du Pont De Nemours And Company Aquaculture meat products
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
TW201207116A (en) * 2010-08-13 2012-02-16 Univ Fu Jen Catholic A method for producing oil by Cystofilobasidium spp.
EP2609202A1 (en) * 2010-08-26 2013-07-03 E.I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
CN103249834B (zh) * 2010-08-26 2016-10-26 纳幕尔杜邦公司 生产高水平二十碳五烯酸的重组微生物宿主细胞
US8980589B2 (en) 2010-08-26 2015-03-17 E I Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
AU2011323288B2 (en) 2010-11-03 2016-05-26 Corbion Biotech, Inc. Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods
JP2014503216A (ja) 2010-12-30 2014-02-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー スクロース利用のための、ヤロウィア・リポリティカ（Ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）におけるサッカロミセス・セレビシア（Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）ＳＵＣ２遺伝子の使用
CA2823210A1 (en) 2010-12-30 2012-07-05 E.I. Du Pont De Nemours And Company Increased oil content by increasing yap1 transcription factor activity in oleaginous yeasts
AU2012212079B2 (en) 2011-02-02 2016-07-28 Corbion Biotech, Inc. Tailored oils produced from recombinant oleaginous microorganisms
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CN103841825B (zh) * 2011-02-11 2017-03-22 纳幕尔杜邦公司 从微生物生物质中获取含脂质组合物的方法
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
EP2691518A1 (en) 2011-03-31 2014-02-05 E.I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US8906650B2 (en) 2011-04-01 2014-12-09 E I Du Pont De Nemours And Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
WO2012138612A1 (en) 2011-04-07 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast
US8785164B2 (en) 2011-05-26 2014-07-22 E I Du Pont De Nemours And Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
US8544772B2 (en) * 2011-05-27 2013-10-01 Ginn Mineral Technology, Inc. Method for the recovery of minerals
WO2013013211A1 (en) * 2011-07-21 2013-01-24 Dsm Ip Assets B.V. Fatty acid compositions
PL2734626T3 (pl) * 2011-07-21 2021-06-28 Dsm Ip Assets B.V. Oleje mikrobiologiczne wzbogacone w wielonienasycone kwasy tłuszczowe
CN104011217B (zh) * 2011-07-21 2018-09-04 帝斯曼知识产权资产管理有限公司 生产二十碳五烯酸的微生物、脂肪酸组合物及其制作方法与用途
CN103031349B (zh) * 2011-09-29 2014-07-09 私立辅仁大学 利用Cystofilobasidium属的酵母菌产生油脂的方法和系统
EP2839018B1 (en) 2012-04-18 2019-06-05 Corbion Biotech, Inc. Tailored oils
CA2877279A1 (en) 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company Improved production of polyunsaturated fatty acids by coexpression of acyl-coa:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
CN102787128A (zh) * 2012-08-28 2012-11-21 江南大学 一种用于多不饱和脂肪酸生物合成的脱饱和酶Δ9 Des
EP2935601B1 (en) 2012-12-21 2018-03-21 E. I. du Pont de Nemours and Company Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight
US9005940B2 (en) 2012-12-21 2015-04-14 E I Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells
US10123986B2 (en) 2012-12-24 2018-11-13 Qualitas Health, Ltd. Eicosapentaenoic acid (EPA) formulations
SG10201709538QA (en) 2012-12-24 2017-12-28 Qualitas Health Ltd Eicosapentaenoic acid (epa) formulations
US9629820B2 (en) 2012-12-24 2017-04-25 Qualitas Health, Ltd. Eicosapentaenoic acid (EPA) formulations
US9896691B2 (en) 2013-05-03 2018-02-20 Board Of Regents, The University Of Texas System Compositions and methods for lipid production
CN105829521A (zh) 2013-10-04 2016-08-03 索拉兹米公司 定制油
CN103749744A (zh) * 2014-02-24 2014-04-30 四川省三台县通宇食品有限公司 一种富含氨基酸食用菌油的制备方法
EP3167053B1 (en) 2014-07-10 2019-10-09 Corbion Biotech, Inc. Novel ketoacyl acp synthase genes and uses thereof
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
IL241841B (en) * 2014-09-30 2019-09-26 Univ Free State Production of proteins for human and animal health using yarrowia lipolytica
US11168369B2 (en) * 2014-11-25 2021-11-09 The Brigham And Women's Hospital, Inc. Method of identifying and treating a person having a predisposition to or afflicted with a cardiometabolic disease
US10683552B2 (en) 2014-11-25 2020-06-16 Presidents And Fellows Of Harvard College Clonal haematopoiesis
EP3267990A4 (en) 2015-03-10 2018-09-05 ELC Management LLC Methods and compositions for treating skin to resolve inflammation and screening for actives that stimulate pro-resolution pathways
CN104805027B (zh) * 2015-05-27 2018-04-27 天津大学 一种重组耶氏解脂酵母菌株及其构建方法和应用
WO2017015368A1 (en) 2015-07-22 2017-01-26 E I Du Pont De Nemours And Company High level production of long-chain dicarboxylic acids with microbes
GB201602410D0 (en) 2016-02-10 2016-03-23 Tate & Lyle Ingredients Methods for improving the gastrointestinal tolerance of food and beverage products comprising sweet, low-digestible carbohydrates
US20180192669A1 (en) 2015-10-01 2018-07-12 Dsm Ip Assets B.V. Supplement material for use in pet food
FI4144844T3 (fi) 2015-10-12 2025-11-24 Dupont Us Holding Llc Suojatut dna-templaatit geenimuokkaukseen ja homologisen rekombinaation lisäämiseen soluissa ja niiden käyttömenetelmät
CN106190872B (zh) * 2016-09-23 2019-10-25 厦门大学 发酵罐底物流加生产二十二碳六烯酸的方法
HUE060726T2 (hu) 2016-12-16 2023-04-28 Univ Danmarks Tekniske Telítetlen zsíralkoholok és telítetlen zsír-acil-acetátok elõállítása élesztõben
DE17825464T1 (de) 2016-12-16 2019-12-19 Danmarks Tekniske Universitet Verfahren zur herstellung von fettalkoholen und derivaten davon in hefe
EP3348647A1 (en) 2017-01-13 2018-07-18 Institut National De La Recherche Agronomique Mutant yeast strain capable of producing medium chain fatty acids
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
CN107502601B (zh) * 2017-06-13 2020-04-21 天津科技大学 一种胰蛋白酶抗性改良的脂肪酶突变体及其基因和应用
WO2019143660A1 (en) * 2018-01-16 2019-07-25 Trustees Of Boston University Clonal hematopoiesis and cytokine targets
US11913046B2 (en) 2018-04-23 2024-02-27 Inbiose N.V. Increasing export of 2'fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
WO2020141206A1 (en) 2019-01-03 2020-07-09 Corbion Biotech Inc. Process for manufacturing lysed cell suspension
CN110499259B (zh) * 2019-07-22 2021-07-27 浙江工业大学 一种解酯耶氏酵母yw100-1及其应用
US11572546B2 (en) 2019-07-29 2023-02-07 Ginkgo Bioworks, Inc. Methods and compositions involving promoters derived from Yarrowia lipolytica
WO2022235655A1 (en) 2021-05-04 2022-11-10 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
EP4334364A1 (en) 2021-05-04 2024-03-13 Nutrition & Biosciences USA 4, Inc. Compositions comprising oxidized insoluble alpha-glucan
EP4463519A1 (en) 2022-01-12 2024-11-20 Nutrition & Biosciences USA 4, Inc. Coating compositions comprising rubber and insoluble alpha-glucan
US20250188647A1 (en) 2022-03-21 2025-06-12 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
EP4627090A1 (en) * 2022-12-02 2025-10-08 Mara Renewables Corporation Oil compositions with engineered lipid profiles and methods of producing same
CN116121093B (zh) * 2022-12-12 2025-11-07 浙江大学杭州国际科创中心 一种用于表达细胞色素p450的工程酵母菌株及其应用
WO2025207802A1 (en) 2024-03-27 2025-10-02 Nutrition & Biosciences USA 4, Inc. Recombinant microbial strains comprising reduced beta-oxidation phenotypes and methods thereof
CN118703533B (zh) * 2024-08-01 2025-11-21 湖南农业大学 OsGAT3基因在调控水稻Cd、Cu或Mn含量及株高中的应用

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3783396T2 (de) * 1986-07-08 1993-07-29 Suntory Ltd Verfahren zur herstellung von bishomo-gamma-linolensaeure und eicosapentaensaeure.
US5246841A (en) * 1986-12-26 1993-09-21 Sagami Chemical Research Center Microbial process for production of eicosapentaenoic acid
US5244921A (en) * 1990-03-21 1993-09-14 Martek Corporation Eicosapentaenoic acids and methods for their production
US5246842A (en) * 1991-10-30 1993-09-21 The United States Of America As Represented By The Secretary Of Agriculture Production of eicosapentaenoic acid from filamentous fungi utilizing lactose as a primary carbon source
JP4151853B2 (ja) * 1996-10-11 2008-09-17 スカリスタ リミテッド エイコサペンタエン酸および／またはステアリドン酸を含む治療用薬剤
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6825017B1 (en) * 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
ES2265865T3 (es) * 1999-09-30 2007-03-01 Loders Croklaan B.V. Composiciones que contienen acido pinolenico y su uso como un componente para la salud.
EP1279400A1 (en) 2001-07-25 2003-01-29 Target Hit sa Modifying the fatty acid composition of cell membranes of organs and tissues
US7410663B2 (en) * 2002-08-07 2008-08-12 Kao Corporation Oil or fat composition
WO2004018598A1 (ja) 2002-08-07 2004-03-04 Kao Corporation 油脂組成物
BR0317609B1 (pt) 2002-12-20 2013-10-08 Textura laminada com tufos
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US8313911B2 (en) * 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7504259B2 (en) * 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
WO2006012325A1 (en) 2004-06-25 2006-02-02 E.I. Dupont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7465564B2 (en) 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7588931B2 (en) * 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
CA2624616A1 (en) * 2005-05-12 2006-11-23 Martek Biosciences Corporation Biomass hydrolysate and uses and production thereof
FR2888976B1 (fr) 2005-07-25 2007-10-12 Oberthur Card Syst Sa Entite electronique avec moyens de communication par contact et a distance
KR20080071190A (ko) * 2005-11-23 2008-08-01 이 아이 듀폰 디 네모아 앤드 캄파니 델타-９ 일롱가제, 및 다중불포화 지방산 생성에 있어서의이들의 용도
US20070225370A1 (en) * 2006-03-21 2007-09-27 Joar Opheim Nutritional Compositions and Methods
US7943823B2 (en) * 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
MX2009010574A (es) * 2007-04-03 2009-10-22 Du Pont Multienzimas y su uso en la fabricacion de acidos grasos poliinsaturados.
US8188338B2 (en) * 2007-04-10 2012-05-29 E. I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids

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