CN101970638B - 用于高水平生产二十碳五烯酸的优化解脂耶氏酵母菌株 - Google Patents
用于高水平生产二十碳五烯酸的优化解脂耶氏酵母菌株 Download PDFInfo
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- CN101970638B CN101970638B CN200880118875.4A CN200880118875A CN101970638B CN 101970638 B CN101970638 B CN 101970638B CN 200880118875 A CN200880118875 A CN 200880118875A CN 101970638 B CN101970638 B CN 101970638B
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- seq
- desaturase
- gene
- epa
- yarrowia lipolytica
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
- A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
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- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
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- A61P9/12—Antihypertensives
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Diabetes (AREA)
- Molecular Biology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Rheumatology (AREA)
- Physical Education & Sports Medicine (AREA)
- Psychiatry (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Mycology (AREA)
- Endocrinology (AREA)
- Pain & Pain Management (AREA)
- Biophysics (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97717407P | 2007-10-03 | 2007-10-03 | |
| US97717707P | 2007-10-03 | 2007-10-03 | |
| US60/977177 | 2007-10-03 | ||
| US60/977174 | 2007-10-03 | ||
| PCT/US2008/078642 WO2009046231A1 (en) | 2007-10-03 | 2008-10-03 | Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101970638A CN101970638A (zh) | 2011-02-09 |
| CN101970638B true CN101970638B (zh) | 2015-02-11 |
Family
ID=40084411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200880118875.4A Expired - Fee Related CN101970638B (zh) | 2007-10-03 | 2008-10-03 | 用于高水平生产二十碳五烯酸的优化解脂耶氏酵母菌株 |
Country Status (6)
| Country | Link |
|---|---|
| US (5) | US8323935B2 (enExample) |
| EP (1) | EP2195415A1 (enExample) |
| JP (1) | JP5711968B2 (enExample) |
| CN (1) | CN101970638B (enExample) |
| CA (1) | CA2701094A1 (enExample) |
| WO (1) | WO2009046231A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107502601A (zh) * | 2017-06-13 | 2017-12-22 | 天津科技大学 | 一种胰蛋白酶抗性改良的脂肪酶突变体及其基因和应用 |
Families Citing this family (82)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8313911B2 (en) * | 2003-05-07 | 2012-11-20 | E I Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US8119860B2 (en) * | 2007-04-16 | 2012-02-21 | E. I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
| US7943365B2 (en) * | 2007-05-03 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Δ-5 desaturases and their use in making polyunsaturated fatty acids |
| JP2010528627A (ja) | 2007-06-01 | 2010-08-26 | ソラザイム、インク | 微生物による油の生産 |
| EP2324120B1 (en) | 2008-08-29 | 2016-01-27 | E. I. du Pont de Nemours and Company | Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms |
| DK2324119T3 (en) * | 2008-09-19 | 2016-07-18 | Du Pont | Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids |
| CA2744716C (en) | 2008-12-18 | 2017-05-23 | E.I. Du Pont De Nemours And Company | Reducing byproduction of malonates in a fermentation process |
| US8207363B2 (en) * | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
| CN102803289B (zh) * | 2009-06-16 | 2015-07-22 | 纳幕尔杜邦公司 | 用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株 |
| US8399226B2 (en) * | 2009-06-16 | 2013-03-19 | E I Du Pont De Nemours And Company | High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica |
| WO2010147907A1 (en) | 2009-06-16 | 2010-12-23 | E. I. Du Pont De Nemours And Company | High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica |
| BRPI1009690A2 (pt) * | 2009-06-16 | 2015-08-25 | Du Pont | "célula hospedeira microbiana oleaginosa, óleo, método de elaboração de um óleo, método de aumento da eficiência de conversão de alongamento de c18 para c20 em uma célula hospedeira recombinante microbiana oleaginosa e método de aumento da eficiência de conversão de dessaturação (delta) 4 em uma célula hospedeira recombinante microbiana oleaginosa produtora de ácidos graxos póli-insaturados de cadeia longa" |
| WO2011087981A2 (en) | 2010-01-15 | 2011-07-21 | E. I. Du Pont De Nemours And Company | Clinical benefits of eicosapentaenoic acid in humans |
| CA2993690C (en) | 2010-01-19 | 2022-03-22 | Dsm Ip Assets B.V. | Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof |
| CN103282474A (zh) | 2010-04-22 | 2013-09-04 | 纳幕尔杜邦公司 | 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法 |
| SG10201504197SA (en) * | 2010-05-28 | 2015-06-29 | Solazyme Inc | Food Compositions Comprising Tailored Oils |
| JP5678180B2 (ja) | 2010-06-09 | 2015-02-25 | フォトンズ コーポレーション リミテッド | 高度精製に適した、エイコサペンタエン酸を含む組成物 |
| WO2012021686A1 (en) | 2010-08-11 | 2012-02-16 | E. I. Du Pont De Nemours And Company | Improved aquaculture meat products |
| US20120040076A1 (en) | 2010-08-11 | 2012-02-16 | E. I. Du Pont De Nemours And Company | Aquaculture feed compositions |
| TW201207116A (en) | 2010-08-13 | 2012-02-16 | Univ Fu Jen Catholic | A method for producing oil by Cystofilobasidium spp. |
| WO2012027676A1 (en) | 2010-08-26 | 2012-03-01 | E. I. Du Pont De Nemours And Company | Mutant delta-9 elongases and their use in making polyunsaturated fatty acids |
| US8703473B2 (en) | 2010-08-26 | 2014-04-22 | E I Du Pont De Nemours And Company | Recombinant microbial host cells for high eicosapentaenoic acid production |
| AU2011293189B2 (en) * | 2010-08-26 | 2017-02-16 | E. I. Du Pont De Nemours And Company | Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids |
| EP3521408B1 (en) | 2010-11-03 | 2021-12-22 | Corbion Biotech, Inc. | Genetically-engineered chlorella or prototheca microbe and oil produced therefrom |
| CN103502461A (zh) | 2010-12-30 | 2014-01-08 | 纳幕尔杜邦公司 | 在含油酵母中通过提高yap1转录因子活性提高油含量 |
| BR112013016769A2 (pt) | 2010-12-30 | 2016-10-11 | Du Pont | yarrowia lipolytica transformada e método para produzir pelo menos um produto não nativo de interesse |
| CN110066836A (zh) | 2011-02-02 | 2019-07-30 | 柯碧恩生物技术公司 | 产自重组产油微生物的定制油 |
| US20130040340A1 (en) | 2011-02-07 | 2013-02-14 | E. I. Du Pont De Nemours And Company | Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms |
| JP6061866B2 (ja) * | 2011-02-11 | 2017-01-18 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company | 微生物バイオマスから脂質含有組成物を得る方法 |
| US8759044B2 (en) | 2011-03-23 | 2014-06-24 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
| US8765425B2 (en) | 2011-03-23 | 2014-07-01 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
| US8969049B2 (en) | 2011-03-31 | 2015-03-03 | E I Du Pont De Nemours And Company | Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast |
| US20120247066A1 (en) | 2011-04-01 | 2012-10-04 | Ice House America, Llc | Ice bagging apparatus and methods |
| EP2694665A1 (en) | 2011-04-01 | 2014-02-12 | E. I. Du Pont de Nemours and Company | Yarrowia esterase/lipase promoter regions for gene expression in yeast |
| WO2012138613A1 (en) | 2011-04-05 | 2012-10-11 | E. I. Du Pont De Nemours And Company | Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast |
| EP2702148A1 (en) | 2011-04-07 | 2014-03-05 | E.I. Du Pont De Nemours And Company | Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast |
| EP2714724A1 (en) | 2011-05-26 | 2014-04-09 | E. I. Du Pont de Nemours and Company | Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein |
| US8544772B2 (en) * | 2011-05-27 | 2013-10-01 | Ginn Mineral Technology, Inc. | Method for the recovery of minerals |
| CN105255738B (zh) * | 2011-07-21 | 2024-12-27 | 帝斯曼知识产权资产管理有限公司 | 生产二十碳五烯酸的微生物、脂肪酸组合物及其制作方法与用途 |
| EA035287B1 (ru) * | 2011-07-21 | 2020-05-25 | ДСМ АйПи АССЕТС Б.В. | Микроорганизмы, продуцирующие эйкозапентаеновую кислоту, композиции, содержащие жирные кислоты, и способы их получения и применения |
| ES2857173T3 (es) * | 2011-07-21 | 2021-09-28 | Dsm Ip Assets Bv | Aceites microbianos enriquecidos en ácidos grasos poliinsaturados |
| CN103031349B (zh) * | 2011-09-29 | 2014-07-09 | 私立辅仁大学 | 利用Cystofilobasidium属的酵母菌产生油脂的方法和系统 |
| BR112014025719A8 (pt) | 2012-04-18 | 2017-10-03 | Solazyme Inc | Óleos customizados |
| WO2013192007A1 (en) | 2012-06-19 | 2013-12-27 | E. I. Du Pont De Nemours And Company | MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES |
| CN102787128A (zh) * | 2012-08-28 | 2012-11-21 | 江南大学 | 一种用于多不饱和脂肪酸生物合成的脱饱和酶Δ9 Des |
| US9701989B2 (en) | 2012-12-21 | 2017-07-11 | E I Du Pont De Nemours And Company | Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight |
| CA2892516A1 (en) | 2012-12-21 | 2014-06-26 | E. I. Du Pont De Nemours And Company | Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells |
| US10123986B2 (en) | 2012-12-24 | 2018-11-13 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
| MX372724B (es) | 2012-12-24 | 2020-06-19 | Qualitas Health Inc | Formulaciones de acido eicosapentaenoico (epa). |
| US9629820B2 (en) | 2012-12-24 | 2017-04-25 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
| CN105431529A (zh) | 2013-05-03 | 2016-03-23 | 德克萨斯大学系统董事会 | 用于真菌脂质生产的组合物和方法 |
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| CN107502601A (zh) * | 2017-06-13 | 2017-12-22 | 天津科技大学 | 一种胰蛋白酶抗性改良的脂肪酶突变体及其基因和应用 |
| CN107502601B (zh) * | 2017-06-13 | 2020-04-21 | 天津科技大学 | 一种胰蛋白酶抗性改良的脂肪酶突变体及其基因和应用 |
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| US20150024441A1 (en) | 2015-01-22 |
| JP5711968B2 (ja) | 2015-05-07 |
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