JP5497986B2 - 突然変異体ａｏｘ１プロモーター - Google Patents

突然変異体ａｏｘ１プロモーター Download PDF

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Publication number
JP5497986B2
JP5497986B2 JP2007556461A JP2007556461A JP5497986B2 JP 5497986 B2 JP5497986 B2 JP 5497986B2 JP 2007556461 A JP2007556461 A JP 2007556461A JP 2007556461 A JP2007556461 A JP 2007556461A JP 5497986 B2 JP5497986 B2 JP 5497986B2
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promoter
cell
nucleic acid
aox1
pichia pastoris
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Japanese (ja)
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JP2008531000A (ja
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フランツ・ハルトナー
アントン・グリーダー
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VTU Holding GmbH
Technische Universitaet Graz
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VTU Holding GmbH
Technische Universitaet Graz
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03013—Alcohol oxidase (1.1.3.13)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2007556461A 2005-02-23 2006-02-23 突然変異体ａｏｘ１プロモーター Expired - Lifetime JP5497986B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ATA304/2005 2005-02-23
AT0030405A AT501955B1 (de) 2005-02-23 2005-02-23 Mutierte aox1-promotoren
PCT/AT2006/000079 WO2006089329A2 (en) 2005-02-23 2006-02-23 Mutant aox 1 promoters

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2011245688A Division JP5552107B2 (ja) 2005-02-23 2011-11-09 突然変異体ａｏｘ１プロモーター

Publications (2)

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JP2008531000A JP2008531000A (ja) 2008-08-14
JP5497986B2 true JP5497986B2 (ja) 2014-05-21

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JP2007556461A Expired - Lifetime JP5497986B2 (ja) 2005-02-23 2006-02-23 突然変異体ａｏｘ１プロモーター
JP2011245688A Expired - Lifetime JP5552107B2 (ja) 2005-02-23 2011-11-09 突然変異体ａｏｘ１プロモーター

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US (2) US9012175B2 (enExample)
EP (2) EP2199389B1 (enExample)
JP (2) JP5497986B2 (enExample)
KR (2) KR101352399B1 (enExample)
CN (2) CN101935657B (enExample)
AT (3) AT501955B1 (enExample)
AU (2) AU2006216094B2 (enExample)
BR (1) BRPI0606179B1 (enExample)
CA (2) CA2598514C (enExample)
DE (2) DE602006018113D1 (enExample)
DK (2) DK2199389T3 (enExample)
ES (2) ES2358086T3 (enExample)
HR (2) HRP20110084T2 (enExample)
IN (1) IN2012DN06694A (enExample)
NZ (2) NZ560542A (enExample)
PL (2) PL1851312T3 (enExample)
PT (1) PT1851312E (enExample)
SI (2) SI2199389T1 (enExample)
UA (2) UA94399C2 (enExample)
WO (1) WO2006089329A2 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5444323B2 (ja) * 2008-03-27 2014-03-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 高発現ザイモモナス（Ｚｙｍｏｍｏｎａｓ）プロモーター
EP2300615A2 (en) * 2008-07-11 2011-03-30 Novozymes A/S Pichia pastoris das promoter variants
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
WO2012129036A2 (en) * 2011-03-22 2012-09-27 Merck Sharp & Dohme Corp. Promoters for high level recombinant expression in fungal host cells
GB201113419D0 (en) * 2011-08-04 2011-09-21 Fujifilm Diosynth Biotechnologies Uk Ltd Yeast vector
EP2565262A1 (en) * 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
AU2012320421B2 (en) 2011-10-07 2017-03-02 Lonza Ltd Regulatable promoter
WO2014088563A1 (en) * 2012-12-05 2014-06-12 Exxonmobil Research And Engineering Company Nannochloropsis kozak consensus sequence
PL2964765T3 (pl) 2013-03-08 2019-10-31 Keck Graduate Inst Of Applied Life Sciences Promotory drożdżowe z pichia pastoris
DK2964775T3 (en) 2013-03-08 2019-04-15 Biogrammatics Inc Yeast promoters for protein expression
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
EP2970994B1 (en) 2013-03-15 2019-07-17 Lonza Ltd Constitutive promoter
CN114365792A (zh) 2014-11-11 2022-04-19 克莱拉食品公司 用于生成卵清蛋白的方法和组合物
HRP20220299T1 (hr) 2015-05-11 2022-05-13 Impossible Foods Inc. Ekspresijski konstrukti i postupci za genetsku modifikaciju metilotrofnog kvasca
WO2017021541A1 (en) 2015-08-05 2017-02-09 Lonza Ltd Promoter variants
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
KR102596200B1 (ko) * 2017-06-30 2023-10-30 다우 글로벌 테크놀로지스 엘엘씨 알데히드 개선을 위한 코팅 및 이의 제조 방법
US11866714B2 (en) 2018-06-07 2024-01-09 Basf Se Promoter for yeast
WO2020068018A1 (en) * 2018-09-24 2020-04-02 Orta Dogu Teknik Universitesi Modified aox1 promoter variants
CN112739819A (zh) * 2018-09-24 2021-04-30 中东科技大学 利用启动子工程化设计醇脱氢酶2(adh2)启动子变体
CN109735547B (zh) * 2019-03-19 2022-05-31 青岛蔚蓝生物集团有限公司 一种提高毕赤酵母外源蛋白表达量的启动子及其应用
CN110029120B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种植酸酶高产菌株及其应用
CN110029122B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种甘露聚糖酶高产菌株及其应用
AU2020257270B2 (en) * 2019-04-17 2026-01-15 Impossible Foods Inc. Materials and methods for protein production
CN110106168B (zh) * 2019-05-27 2021-07-13 西南大学 基于转录因子间的互作关系构建双激素响应启动子的方法
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
ES3015627T3 (en) 2019-07-11 2025-05-06 Clara Foods Co Beverage composition comprising recombinant ovomucoid protein
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
CN110724720A (zh) * 2019-11-01 2020-01-24 山东仙普爱瑞科技股份有限公司 一种猪α-干扰素的发酵工艺
EP3922726A1 (en) * 2020-06-10 2021-12-15 Erber Aktiengesellschaft Truncated promoter for recombinant gene expression
CN117120618A (zh) 2021-02-12 2023-11-24 勃林格殷格翰 Rcv 两合公司 用于增加蛋白质分泌的信号肽
US11512336B1 (en) * 2021-06-24 2022-11-29 BioBoost Synbio Consulting Inc. Method for methanol free culturing of methylotrophic yeast for the biosynthesis of added value products
CN121263531A (zh) 2023-06-06 2026-01-02 富士胶片株式会社 基因重组酵母、目标蛋白质的制造方法、试剂盒及基因重组酵母的制造方法
MX2025015008A (es) * 2023-06-15 2026-02-03 Danstar Ferment Ag Promotores que reaccionan a la fuente de carbono c2
CN117417934B (zh) * 2023-10-27 2024-09-13 江南大学 跨菌种启动子、多菌种穿梭质粒及其应用
WO2025104126A1 (en) 2023-11-14 2025-05-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
WO2025163019A1 (en) 2024-02-01 2025-08-07 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
CN118599913B (zh) * 2024-08-07 2024-12-27 南昌大学 醛氧化酶Aox1基因敲除动物模型的构建方法及应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2063890C (en) * 1991-03-27 2007-03-13 Masami Miura Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
JPH06209763A (ja) * 1993-01-13 1994-08-02 Green Cross Corp:The 変異株
WO1994021802A1 (en) * 1993-03-25 1994-09-29 Biosource Genetics Corporation PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2)
JP3290108B2 (ja) * 1997-08-29 2002-06-10 麒麟麦酒株式会社 プロモーター活性を増大させるｄｎａ配列
CN1192103C (zh) * 1998-01-27 2005-03-09 三井化学株式会社 生产植酸酶的方法
AU3910000A (en) * 1999-03-22 2000-10-09 Zymogenetics Inc. Improved methods for producing proteins in transformed (pichia)
CN1188524C (zh) * 2001-02-28 2005-02-09 珠江医院 人SP－A1在pichia pastoris中的表达
WO2002081650A2 (en) * 2001-04-05 2002-10-17 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
EP1431387B1 (en) * 2002-12-20 2008-02-27 Roche Diagnostics GmbH Heat-labile desoxyribonculease I variants

Also Published As

Publication number Publication date
AT501955B1 (de) 2007-08-15
KR20120106979A (ko) 2012-09-27
PT1851312E (pt) 2011-01-05
EP2199389A1 (en) 2010-06-23
HRP20110084T2 (hr) 2011-10-31
CN101935657A (zh) 2011-01-05
PL1851312T3 (pl) 2011-05-31
BRPI0606179A2 (pt) 2009-06-02
KR101358663B1 (ko) 2014-02-20
EP1851312A2 (en) 2007-11-07
WO2006089329A3 (en) 2007-05-31
EP1851312B9 (en) 2011-04-20
US9279129B2 (en) 2016-03-08
US9012175B2 (en) 2015-04-21
CA2785161C (en) 2020-03-31
CN101128581A (zh) 2008-02-20
ATE496125T1 (de) 2011-02-15
AU2006216094B2 (en) 2010-10-21
SI1851312T1 (sl) 2011-03-31
HK1113172A1 (en) 2008-09-26
UA98604C2 (ru) 2012-05-25
HK1148313A1 (en) 2011-09-02
AU2011200187B2 (en) 2012-01-19
CA2598514A1 (en) 2006-08-31
AU2011200187A1 (en) 2011-02-03
DK1851312T3 (da) 2011-02-28
JP5552107B2 (ja) 2014-07-16
AU2006216094A1 (en) 2006-08-31
KR101352399B1 (ko) 2014-02-18
WO2006089329A2 (en) 2006-08-31
AT501955A1 (de) 2006-12-15
BRPI0606179B1 (pt) 2018-05-29
IN2012DN06694A (enExample) 2015-10-23
UA94399C2 (ru) 2011-05-10
DK1851312T5 (da) 2011-04-18
ES2356127T9 (es) 2011-05-30
WO2006089329A8 (en) 2006-11-02
CA2598514C (en) 2013-07-09
US20100196913A1 (en) 2010-08-05
NZ560542A (en) 2010-06-25
CA2785161A1 (en) 2006-08-31
JP2012080886A (ja) 2012-04-26
HRP20110084T1 (hr) 2011-03-31
HRP20110254T1 (hr) 2011-05-31
US20080153126A1 (en) 2008-06-26
ES2356127T3 (es) 2011-04-05
SI2199389T1 (sl) 2011-05-31
DE602006019789D1 (de) 2011-03-03
NZ585821A (en) 2011-02-25
EP2199389B1 (en) 2011-01-19
DK2199389T3 (da) 2011-04-11
EP1851312B1 (en) 2010-11-10
ES2358086T3 (es) 2011-05-05
KR20070107781A (ko) 2007-11-07
JP2008531000A (ja) 2008-08-14
CN101128581B (zh) 2012-04-18
PL2199389T3 (pl) 2011-06-30
ATE487788T1 (de) 2010-11-15
CN101935657B (zh) 2013-02-13
DE602006018113D1 (de) 2010-12-23

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