CN101128581B - 突变aox1启动子 - Google Patents

突变aox1启动子 Download PDF

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Publication number
CN101128581B
CN101128581B CN2006800058881A CN200680005888A CN101128581B CN 101128581 B CN101128581 B CN 101128581B CN 2006800058881 A CN2006800058881 A CN 2006800058881A CN 200680005888 A CN200680005888 A CN 200680005888A CN 101128581 B CN101128581 B CN 101128581B
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China
Prior art keywords
promoter
nucleotides
nucleic acid
cells
cell
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Expired - Lifetime
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CN2006800058881A
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Chinese (zh)
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CN101128581A (zh
Inventor
弗朗兹·哈特纳
安东·格利德
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Valladogan LLC
Boehringer Ingelheim RCV GmbH and Co KG
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Vtu Holding Co ltd
University Graz Tech
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03013—Alcohol oxidase (1.1.3.13)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
CN2006800058881A 2005-02-23 2006-02-23 突变aox1启动子 Expired - Lifetime CN101128581B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ATA304/2005 2005-02-23
AT0030405A AT501955B1 (de) 2005-02-23 2005-02-23 Mutierte aox1-promotoren
PCT/AT2006/000079 WO2006089329A2 (en) 2005-02-23 2006-02-23 Mutant aox 1 promoters

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN2010101738787A Division CN101935657B (zh) 2005-02-23 2006-02-23 突变aox1启动子

Publications (2)

Publication Number Publication Date
CN101128581A CN101128581A (zh) 2008-02-20
CN101128581B true CN101128581B (zh) 2012-04-18

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CN2010101738787A Expired - Lifetime CN101935657B (zh) 2005-02-23 2006-02-23 突变aox1启动子

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US (2) US9012175B2 (enExample)
EP (2) EP2199389B1 (enExample)
JP (2) JP5497986B2 (enExample)
KR (2) KR101358663B1 (enExample)
CN (2) CN101128581B (enExample)
AT (3) AT501955B1 (enExample)
AU (2) AU2006216094B2 (enExample)
BR (1) BRPI0606179B1 (enExample)
CA (2) CA2785161C (enExample)
DE (2) DE602006019789D1 (enExample)
DK (2) DK2199389T3 (enExample)
ES (2) ES2358086T3 (enExample)
HR (2) HRP20110084T2 (enExample)
IN (1) IN2012DN06694A (enExample)
NZ (2) NZ560542A (enExample)
PL (2) PL2199389T3 (enExample)
PT (1) PT1851312E (enExample)
SI (2) SI2199389T1 (enExample)
UA (2) UA94399C2 (enExample)
WO (1) WO2006089329A2 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009228322B2 (en) * 2008-03-27 2013-09-26 Alliance For Sustainable Energy, Llc High expression Zymomonas promoters
US20110129874A1 (en) * 2008-07-11 2011-06-02 Novozymes A/S Pichia Pastoris Das Promoter Variants
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
WO2012129036A2 (en) * 2011-03-22 2012-09-27 Merck Sharp & Dohme Corp. Promoters for high level recombinant expression in fungal host cells
GB201113419D0 (en) * 2011-08-04 2011-09-21 Fujifilm Diosynth Biotechnologies Uk Ltd Yeast vector
EP2565262A1 (en) 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
KR102128957B1 (ko) 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
EP2931739A4 (en) 2012-12-05 2016-08-03 Exxonmobil Res & Eng Co Nannochloropsis-KOZAK SEQUENCE
BR112015021753B1 (pt) 2013-03-08 2023-02-23 Keck Graduate Institute Of Applied Life Sciences Ácidos nucleicos isolados de pichia pastoris, vetor de expressão, células hospedeiras de levedura e construto de dna, bem como método para produzir uma proteína
DK2964775T3 (en) 2013-03-08 2019-04-15 Biogrammatics Inc Yeast promoters for protein expression
SG11201507557UA (en) 2013-03-15 2015-10-29 Lonza Ag Constitutive promoter
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
HRP20220299T1 (hr) 2015-05-11 2022-05-13 Impossible Foods Inc. Ekspresijski konstrukti i postupci za genetsku modifikaciju metilotrofnog kvasca
CA2992170C (en) 2015-08-05 2022-08-16 Lonza Ltd Artificial pg1-x promoters
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
KR102596200B1 (ko) * 2017-06-30 2023-10-30 다우 글로벌 테크놀로지스 엘엘씨 알데히드 개선을 위한 코팅 및 이의 제조 방법
WO2019236451A1 (en) * 2018-06-07 2019-12-12 Basf Se Promoter for yeast
WO2020068018A1 (en) * 2018-09-24 2020-04-02 Orta Dogu Teknik Universitesi Modified aox1 promoter variants
US12091658B2 (en) 2018-09-24 2024-09-17 Orta Dogu Teknik Universitesi Alcohol dehydrogenase 2 (ADH2) promoter variants by promoter engineering
CN110029120B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种植酸酶高产菌株及其应用
CN109735547B (zh) * 2019-03-19 2022-05-31 青岛蔚蓝生物集团有限公司 一种提高毕赤酵母外源蛋白表达量的启动子及其应用
CN110029122B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种甘露聚糖酶高产菌株及其应用
BR112021020727A2 (pt) * 2019-04-17 2021-12-14 Impossible Foods Inc Materiais e métodos para produção de proteína
CN110106168B (zh) * 2019-05-27 2021-07-13 西南大学 基于转录因子间的互作关系构建双激素响应启动子的方法
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
FI13395Y1 (fi) 2019-07-11 2023-06-06 Clara Foods Co Koostumuksia, jotka käsittävät rekombinanttia ovomukoidiproteiinia
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
CN110724720A (zh) * 2019-11-01 2020-01-24 山东仙普爱瑞科技股份有限公司 一种猪α-干扰素的发酵工艺
EP3922726A1 (en) * 2020-06-10 2021-12-15 Erber Aktiengesellschaft Truncated promoter for recombinant gene expression
EP4291659A1 (en) 2021-02-12 2023-12-20 Boehringer Ingelheim RCV GmbH & Co KG Signal peptides for increased protein secretion
US11512336B1 (en) * 2021-06-24 2022-11-29 BioBoost Synbio Consulting Inc. Method for methanol free culturing of methylotrophic yeast for the biosynthesis of added value products
EP4726051A1 (en) 2023-06-06 2026-04-15 FUJIFILM Corporation Recombinant yeast, method for producing target protein, and kit and method for producing recombinant yeast
WO2024256996A1 (en) * 2023-06-15 2024-12-19 Danstar Ferment Ag C2 carbon source-responsive promoters
CN117417934B (zh) * 2023-10-27 2024-09-13 江南大学 跨菌种启动子、多菌种穿梭质粒及其应用
AU2024379744A1 (en) 2023-11-14 2026-05-07 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
AU2025216822A1 (en) 2024-02-01 2026-07-23 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
CN118599913B (zh) * 2024-08-07 2024-12-27 南昌大学 醛氧化酶Aox1基因敲除动物模型的构建方法及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1372001A (zh) * 2001-02-28 2002-10-02 珠江医院 人SP－A1在pichia pastoris中的表达

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2063890C (en) * 1991-03-27 2007-03-13 Masami Miura Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
JPH06209763A (ja) * 1993-01-13 1994-08-02 Green Cross Corp:The 変異株
WO1994021802A1 (en) * 1993-03-25 1994-09-29 Biosource Genetics Corporation PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2)
JP3290108B2 (ja) * 1997-08-29 2002-06-10 麒麟麦酒株式会社 プロモーター活性を増大させるｄｎａ配列
CN1192103C (zh) * 1998-01-27 2005-03-09 三井化学株式会社 生产植酸酶的方法
WO2000056903A2 (en) * 1999-03-22 2000-09-28 Zymogenetics, Inc. IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)
WO2002081650A2 (en) * 2001-04-05 2002-10-17 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
EP1431387B1 (en) * 2002-12-20 2008-02-27 Roche Diagnostics GmbH Heat-labile desoxyribonculease I variants

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1372001A (zh) * 2001-02-28 2002-10-02 珠江医院 人SP－A1在pichia pastoris中的表达

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
戴秀玉等人.毕赤氏酵母醇氧化酶-2基因启动子突变体的分离和鉴定.遗传学报27 7.2000,27(7),641-646.
戴秀玉等人.毕赤氏酵母醇氧化酶-2基因启动子突变体的分离和鉴定.遗传学报27 7.2000,27(7),641-646. *

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HK1113172A1 (en) 2008-09-26
EP1851312B9 (en) 2011-04-20
KR20120106979A (ko) 2012-09-27
WO2006089329A2 (en) 2006-08-31
AU2006216094A1 (en) 2006-08-31
DK2199389T3 (da) 2011-04-11
EP1851312B1 (en) 2010-11-10
ATE496125T1 (de) 2011-02-15
AU2006216094B2 (en) 2010-10-21
ATE487788T1 (de) 2010-11-15
CA2785161C (en) 2020-03-31
UA94399C2 (ru) 2011-05-10
DE602006018113D1 (de) 2010-12-23
DK1851312T5 (da) 2011-04-18
CN101128581A (zh) 2008-02-20
JP5552107B2 (ja) 2014-07-16
NZ560542A (en) 2010-06-25
JP5497986B2 (ja) 2014-05-21
CA2598514A1 (en) 2006-08-31
EP2199389B1 (en) 2011-01-19
HRP20110084T2 (hr) 2011-10-31
ES2356127T3 (es) 2011-04-05
US20100196913A1 (en) 2010-08-05
DE602006019789D1 (de) 2011-03-03
HK1148313A1 (en) 2011-09-02
EP2199389A1 (en) 2010-06-23
SI2199389T1 (sl) 2011-05-31
AU2011200187A1 (en) 2011-02-03
WO2006089329A3 (en) 2007-05-31
JP2012080886A (ja) 2012-04-26
ES2358086T3 (es) 2011-05-05
PL2199389T3 (pl) 2011-06-30
AT501955B1 (de) 2007-08-15
EP1851312A2 (en) 2007-11-07
IN2012DN06694A (enExample) 2015-10-23
US20080153126A1 (en) 2008-06-26
WO2006089329A8 (en) 2006-11-02
HRP20110254T1 (hr) 2011-05-31
KR101358663B1 (ko) 2014-02-20
DK1851312T3 (da) 2011-02-28
US9012175B2 (en) 2015-04-21
ES2356127T9 (es) 2011-05-30
SI1851312T1 (sl) 2011-03-31
KR20070107781A (ko) 2007-11-07
US9279129B2 (en) 2016-03-08
AT501955A1 (de) 2006-12-15
BRPI0606179B1 (pt) 2018-05-29
NZ585821A (en) 2011-02-25
JP2008531000A (ja) 2008-08-14
CA2785161A1 (en) 2006-08-31
KR101352399B1 (ko) 2014-02-18
CN101935657B (zh) 2013-02-13
BRPI0606179A2 (pt) 2009-06-02
AU2011200187B2 (en) 2012-01-19
UA98604C2 (ru) 2012-05-25
PL1851312T3 (pl) 2011-05-31
CA2598514C (en) 2013-07-09
PT1851312E (pt) 2011-01-05
CN101935657A (zh) 2011-01-05
HRP20110084T1 (hr) 2011-03-31

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