BRPI0606179A2 - promotores de aox1 mutantes - Google Patents
promotores de aox1 mutantesInfo
- Publication number
- BRPI0606179A2 BRPI0606179A2 BRPI0606179-6A BRPI0606179A BRPI0606179A2 BR PI0606179 A2 BRPI0606179 A2 BR PI0606179A2 BR PI0606179 A BRPI0606179 A BR PI0606179A BR PI0606179 A2 BRPI0606179 A2 BR PI0606179A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleotides
- mutant
- seq
- aox1 promoters
- pichia pastoris
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
- C12Y101/03013—Alcohol oxidase (1.1.3.13)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
PROMOTORES DE AOX1 MUTANTES. A presente invenção refere-se a um promotor de álcool oxidase 1 (AOX1) de Pichia pastoris mutante, do promotor de AOX1 de Pichia pastoris de tipo selvagem (SEQ ID N 1), compreendendo pelo menos uma mutação selecionada a partir do grupo consistindo em: a) um sítio de ligação a fator de transcrição (TFBS), b) nucleotídeos 170 a 235 (-784 a -719), nucleotídeos 170 a 191 (-784 a -763), nucleotídeos 192 a 213 (-762 a -741), nucleotídeos 192 a 210 (-762 a -744), nucleotídeos 207 a 209 (-747 a -745), nucleotídeos 214 a 235 (-740 a -719), nucleotídeos 304 a 350 (-650 a -604), nucleotídeos 364 a 393 (-590 a -561), nucleotídeos 434 a 508 (-520 a -446), nucleotídeos 509 a 551 (-445 a -403), nucleotídeos 552 a 560 (-402 a -394), nucleotídeos 585 a 617 (-369 a -337), nucleotídeos 621 a 660 (-333 a -294), nucleotídeos 625 a 683 (-329 a -271), nucleotídeos 736 a 741 (-218 a -213), nucleotídeos 737 a 738 (-217 a -216), nucleotídeos 726 a 755 (-228 a -199), nucleotídeos 784 a 800 (-170 a -154) ou nucleotídeos 823 a 861 (-131 a -93) de SEQ ID N 1, e suas combinações.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ATA304/2005 | 2005-02-23 | ||
| AT0030405A AT501955B1 (de) | 2005-02-23 | 2005-02-23 | Mutierte aox1-promotoren |
| PCT/AT2006/000079 WO2006089329A2 (en) | 2005-02-23 | 2006-02-23 | Mutant aox 1 promoters |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BRPI0606179A2 true BRPI0606179A2 (pt) | 2009-06-02 |
| BRPI0606179B1 BRPI0606179B1 (pt) | 2018-05-29 |
Family
ID=36927772
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0606179-6A BRPI0606179B1 (pt) | 2005-02-23 | 2006-02-23 | Promotor isolado de álcool oxidase 1 (aox1) de pichia pastoris, vetor, célula, kit, método para a expressão de uma proteína recombinante, peptídeo ou ácido nucléico funcional, em uma célula, método para a expressão de uma molécula de ácido nucleico para a expressão de uma proteína, peptídeo ou ácido nucléico funcional, e método para o isolamento de clones de superexpressão |
Country Status (21)
| Country | Link |
|---|---|
| US (2) | US9012175B2 (pt) |
| EP (2) | EP1851312B9 (pt) |
| JP (2) | JP5497986B2 (pt) |
| KR (2) | KR101358663B1 (pt) |
| CN (2) | CN101128581B (pt) |
| AT (3) | AT501955B1 (pt) |
| AU (2) | AU2006216094B2 (pt) |
| BR (1) | BRPI0606179B1 (pt) |
| CA (2) | CA2785161C (pt) |
| DE (2) | DE602006019789D1 (pt) |
| DK (2) | DK2199389T3 (pt) |
| ES (2) | ES2358086T3 (pt) |
| HK (2) | HK1148313A1 (pt) |
| HR (2) | HRP20110084T2 (pt) |
| IN (1) | IN2012DN06694A (pt) |
| NZ (2) | NZ585821A (pt) |
| PL (2) | PL2199389T3 (pt) |
| PT (1) | PT1851312E (pt) |
| SI (2) | SI1851312T1 (pt) |
| UA (2) | UA94399C2 (pt) |
| WO (1) | WO2006089329A2 (pt) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7989206B2 (en) * | 2008-03-27 | 2011-08-02 | E.I. du Pont de Nemours and Company Alliance for Sustainable Energy LLC | High expression Zymomonas promoters |
| EP2300615A2 (en) * | 2008-07-11 | 2011-03-30 | Novozymes A/S | Pichia pastoris das promoter variants |
| EP2258855A1 (en) | 2009-05-28 | 2010-12-08 | Universität für Bodenkultur Wien | Expression sequences |
| US20140011236A1 (en) * | 2011-03-22 | 2014-01-09 | Merch Sharp & Dohme Corp. | Promoters for high level recombinant expression in fungal host cells |
| GB201113419D0 (en) * | 2011-08-04 | 2011-09-21 | Fujifilm Diosynth Biotechnologies Uk Ltd | Yeast vector |
| EP2565262A1 (en) | 2011-08-31 | 2013-03-06 | VTU Holding GmbH | Protein expression |
| EP3508494B1 (en) * | 2011-10-07 | 2022-02-23 | Lonza Ltd | Regulatable promoter |
| WO2014088563A1 (en) * | 2012-12-05 | 2014-06-12 | Exxonmobil Research And Engineering Company | Nannochloropsis kozak consensus sequence |
| EP3839055B1 (en) * | 2013-03-08 | 2023-08-23 | Biogrammatics, Inc. | Yeast promoters for protein expression |
| DK3594351T3 (da) | 2013-03-08 | 2022-01-03 | Keck Graduate Inst Of Applied Life Sciences | Gærpromotorer fra pichia pastoris |
| ES2751336T3 (es) | 2013-03-15 | 2020-03-31 | Lonza Ag | Promotor constitutivo |
| US9150870B2 (en) | 2013-03-15 | 2015-10-06 | Lonza Ltd. | Constitutive promoter |
| US11518797B2 (en) | 2014-11-11 | 2022-12-06 | Clara Foods Co. | Methods and compositions for egg white protein production |
| JP6833812B2 (ja) | 2015-08-05 | 2021-02-24 | ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ | プロモーター変異体 |
| CN108728477B (zh) * | 2017-04-24 | 2022-02-22 | 华东理工大学 | 一种高效的转座突变系统及构建方法 |
| AU2018291000B2 (en) * | 2017-06-30 | 2022-10-27 | Dow Global Technologies Llc | Coating for aldehyde remediation and method of making |
| EP3802832A1 (en) * | 2018-06-07 | 2021-04-14 | Basf Se | Promoter for yeast |
| JP2022501028A (ja) * | 2018-09-24 | 2022-01-06 | オルタ ドグ テクニク ユニヴェルシテシ | プロモータ工学によるアルコールデヒドロゲナーゼ２（ａｄｈ２）プロモータ変異体の設計 |
| WO2020068018A1 (en) * | 2018-09-24 | 2020-04-02 | Orta Dogu Teknik Universitesi | Modified aox1 promoter variants |
| CN110029120B (zh) * | 2019-03-19 | 2022-03-01 | 青岛蔚蓝生物集团有限公司 | 一种植酸酶高产菌株及其应用 |
| CN109735547B (zh) * | 2019-03-19 | 2022-05-31 | 青岛蔚蓝生物集团有限公司 | 一种提高毕赤酵母外源蛋白表达量的启动子及其应用 |
| CN110029122B (zh) * | 2019-03-19 | 2022-03-01 | 青岛蔚蓝生物集团有限公司 | 一种甘露聚糖酶高产菌株及其应用 |
| US11427932B2 (en) * | 2019-04-17 | 2022-08-30 | Impossible Foods Inc. | Materials and methods for protein production |
| CN110106168B (zh) * | 2019-05-27 | 2021-07-13 | 西南大学 | 基于转录因子间的互作关系构建双激素响应启动子的方法 |
| EP3997118A4 (en) | 2019-07-11 | 2023-07-19 | Clara Foods Co. | PROTEIN COMPOSITIONS AND CONSUMABLE PRODUCTS THEREOF |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CN110724720A (zh) * | 2019-11-01 | 2020-01-24 | 山东仙普爱瑞科技股份有限公司 | 一种猪α-干扰素的发酵工艺 |
| WO2022171827A1 (en) | 2021-02-12 | 2022-08-18 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Signal peptides for increased protein secretion |
| CA3131181C (en) * | 2021-06-24 | 2023-03-07 | BioBoost Synbio Consulting Inc. | Method for methanol free culturing of methylotrophic yeast for the biosynthesis of added value products |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2063890C (en) * | 1991-03-27 | 2007-03-13 | Masami Miura | Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism |
| CA2058820C (en) * | 1991-04-25 | 2003-07-15 | Kotikanyad Sreekrishna | Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells |
| JPH06209763A (ja) * | 1993-01-13 | 1994-08-02 | Green Cross Corp:The | 変異株 |
| WO1994021802A1 (en) * | 1993-03-25 | 1994-09-29 | Biosource Genetics Corporation | PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2) |
| JP3290108B2 (ja) * | 1997-08-29 | 2002-06-10 | 麒麟麦酒株式会社 | プロモーター活性を増大させるｄｎａ配列 |
| CN1192103C (zh) * | 1998-01-27 | 2005-03-09 | 三井化学株式会社 | 生产植酸酶的方法 |
| WO2000056903A2 (en) * | 1999-03-22 | 2000-09-28 | Zymogenetics, Inc. | IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) |
| CN1188524C (zh) * | 2001-02-28 | 2005-02-09 | 珠江医院 | 人SP－A1在pichia pastoris中的表达 |
| CA2442935A1 (en) * | 2001-04-05 | 2002-10-17 | The Board Of Regents Of The University Of Nebraska | Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast |
| EP1431387B1 (en) * | 2002-12-20 | 2008-02-27 | Roche Diagnostics GmbH | Heat-labile desoxyribonculease I variants |
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2005
- 2005-02-23 AT AT0030405A patent/AT501955B1/de not_active IP Right Cessation
-
2006
- 2006-02-23 EP EP06704721A patent/EP1851312B9/en active Active
- 2006-02-23 EP EP10155180A patent/EP2199389B1/en active Active
- 2006-02-23 CN CN2006800058881A patent/CN101128581B/zh active Active
- 2006-02-23 ES ES10155180T patent/ES2358086T3/es active Active
- 2006-02-23 WO PCT/AT2006/000079 patent/WO2006089329A2/en active Application Filing
- 2006-02-23 CA CA2785161A patent/CA2785161C/en active Active
- 2006-02-23 NZ NZ585821A patent/NZ585821A/en unknown
- 2006-02-23 KR KR1020127018581A patent/KR101358663B1/ko active IP Right Grant
- 2006-02-23 DE DE602006019789T patent/DE602006019789D1/de active Active
- 2006-02-23 AU AU2006216094A patent/AU2006216094B2/en active Active
- 2006-02-23 NZ NZ560542A patent/NZ560542A/en unknown
- 2006-02-23 DK DK10155180.2T patent/DK2199389T3/da active
- 2006-02-23 BR BRPI0606179-6A patent/BRPI0606179B1/pt active IP Right Grant
- 2006-02-23 KR KR1020077021821A patent/KR101352399B1/ko active IP Right Grant
- 2006-02-23 CA CA2598514A patent/CA2598514C/en active Active
- 2006-02-23 PL PL10155180T patent/PL2199389T3/pl unknown
- 2006-02-23 ES ES06704721T patent/ES2356127T3/es active Active
- 2006-02-23 SI SI200630919T patent/SI1851312T1/sl unknown
- 2006-02-23 UA UAA200710436A patent/UA94399C2/ru unknown
- 2006-02-23 PT PT06704721T patent/PT1851312E/pt unknown
- 2006-02-23 PL PL06704721T patent/PL1851312T3/pl unknown
- 2006-02-23 DK DK06704721.7T patent/DK1851312T5/da active
- 2006-02-23 JP JP2007556461A patent/JP5497986B2/ja active Active
- 2006-02-23 DE DE602006018113T patent/DE602006018113D1/de active Active
- 2006-02-23 US US11/817,005 patent/US9012175B2/en active Active
- 2006-02-23 SI SI200630962T patent/SI2199389T1/sl unknown
- 2006-02-23 UA UAA201012563A patent/UA98604C2/ru unknown
- 2006-02-23 AT AT06704721T patent/ATE487788T1/de active
- 2006-02-23 IN IN6694DEN2012 patent/IN2012DN06694A/en unknown
- 2006-02-23 CN CN2010101738787A patent/CN101935657B/zh active Active
- 2006-02-23 AT AT10155180T patent/ATE496125T1/de active
-
2008
- 2008-08-08 HK HK11102457.9A patent/HK1148313A1/xx not_active IP Right Cessation
- 2008-08-08 HK HK08108778.3A patent/HK1113172A1/xx not_active IP Right Cessation
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2010
- 2010-04-14 US US12/759,763 patent/US9279129B2/en active Active
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2011
- 2011-01-18 AU AU2011200187A patent/AU2011200187B2/en active Active
- 2011-02-03 HR HR20110084T patent/HRP20110084T2/hr unknown
- 2011-04-08 HR HR20110254T patent/HRP20110254T1/hr unknown
- 2011-11-09 JP JP2011245688A patent/JP5552107B2/ja active Active
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Legal Events
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| B25D | Requested change of name of applicant approved |
Owner name: TECHNISCHE UNIVERSITAET GRAZ (AT) , VTU EQUITY GMB Free format text: ALTERADO DE: VTU-ENGINEERING PLANUNGS- UND BERATUNGSGESELLSCHAFT M.B.H |
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| B25A | Requested transfer of rights approved |
Owner name: TECHNISCHE UNIVERSITAET GRAZ (AT) , VTU HOLDING GM Free format text: TRANSFERIDO DE: VTU EQUITY GMBH |
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| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 29/05/2018, OBSERVADAS AS CONDICOES LEGAIS. |