WO2005085451A3 - Polynucleotide phosphorylase (pnpase) as target for herbicides - Google Patents

Polynucleotide phosphorylase (pnpase) as target for herbicides Download PDF

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Publication number
WO2005085451A3
WO2005085451A3 PCT/EP2005/050942 EP2005050942W WO2005085451A3 WO 2005085451 A3 WO2005085451 A3 WO 2005085451A3 EP 2005050942 W EP2005050942 W EP 2005050942W WO 2005085451 A3 WO2005085451 A3 WO 2005085451A3
Authority
WO
WIPO (PCT)
Prior art keywords
herbicides
pnpase
target
polynucleotide phosphorylase
growth
Prior art date
Application number
PCT/EP2005/050942
Other languages
French (fr)
Other versions
WO2005085451A2 (en
Inventor
Frederik Boernke
Thomas Ehrhardt
Annette Freund
Wolfgang Lein
Andreas Reindl
Ralf-Michael Schmidt
Uwe Sonnewald
Nigel Marc Stitt
Original Assignee
Basf Ag
Frederik Boernke
Thomas Ehrhardt
Annette Freund
Wolfgang Lein
Andreas Reindl
Ralf-Michael Schmidt
Uwe Sonnewald
Nigel Marc Stitt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Frederik Boernke, Thomas Ehrhardt, Annette Freund, Wolfgang Lein, Andreas Reindl, Ralf-Michael Schmidt, Uwe Sonnewald, Nigel Marc Stitt filed Critical Basf Ag
Priority to DE112005000590T priority Critical patent/DE112005000590T5/en
Publication of WO2005085451A2 publication Critical patent/WO2005085451A2/en
Publication of WO2005085451A3 publication Critical patent/WO2005085451A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1258—Polyribonucleotide nucleotidyltransferase (2.7.7.8), i.e. polynucleotide phosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Analytical Chemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to polynucleotide phosphorylase (PNPase), which, when absent brings about reduced growth and chlorotic leaves, as target for herbicides. For this purpose, nucleic acid sequences encompassing SEQ. ID No. 1 and functional equivalents of SEQ.ID No. 1 are provided. Moreover, the present invention relates to the use of polynucleotide phosphorylase in a method for identifying compounds with herbicidal or growth-regulatory activity, and to the use of the compounds identified by this method as herbicides or growth regulators.
PCT/EP2005/050942 2004-03-10 2005-02-23 Polynucleotide phosphorylase (pnpase) as target for herbicides WO2005085451A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112005000590T DE112005000590T5 (en) 2004-03-10 2005-02-23 Polynucleotide phosphorylase (PNPase) as a target for herbicides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04005667.3 2004-03-10
EP04005667 2004-03-10

Publications (2)

Publication Number Publication Date
WO2005085451A2 WO2005085451A2 (en) 2005-09-15
WO2005085451A3 true WO2005085451A3 (en) 2006-07-06

Family

ID=34917172

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/050942 WO2005085451A2 (en) 2004-03-10 2005-02-23 Polynucleotide phosphorylase (pnpase) as target for herbicides

Country Status (2)

Country Link
DE (1) DE112005000590T5 (en)
WO (1) WO2005085451A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000000623A1 (en) * 1998-06-30 2000-01-06 Syngenta Participations Ag Hmp-p kinase and tmp-ppase from arabidopsis thaliana and their use in herbicide screening
WO2000077185A2 (en) * 1999-06-15 2000-12-21 Syngenta Participations Ag Herbicide target genes and methods
WO2003025004A2 (en) * 2001-09-18 2003-03-27 Affinium Pharmaceuticals, Inc. Purified bacterial polypeptides involved in nucleic acid processing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000000623A1 (en) * 1998-06-30 2000-01-06 Syngenta Participations Ag Hmp-p kinase and tmp-ppase from arabidopsis thaliana and their use in herbicide screening
WO2000077185A2 (en) * 1999-06-15 2000-12-21 Syngenta Participations Ag Herbicide target genes and methods
WO2003025004A2 (en) * 2001-09-18 2003-03-27 Affinium Pharmaceuticals, Inc. Purified bacterial polypeptides involved in nucleic acid processing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LI QING-SHUN ET AL: "Polynucleotide phosphorylase is a component of a novel plant poly(A) polymerase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 28, 10 July 1998 (1998-07-10), pages 17539 - 17543, XP002293936, ISSN: 0021-9258 *
REUVEN NINA BACHER ET AL: "Functional overlap of tRNA nucleotidyltransferase, poly(A) polymerase I, and polynucleotide phosphorylase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 52, 26 December 1997 (1997-12-26), pages 33255 - 33259, XP002293937, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2005085451A2 (en) 2005-09-15
DE112005000590T5 (en) 2007-04-26

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