WO2006086669A8 - Aptamer regulated nucleic acids and uses thereof - Google Patents

Aptamer regulated nucleic acids and uses thereof

Info

Publication number
WO2006086669A8
WO2006086669A8 PCT/US2006/004801 US2006004801W WO2006086669A8 WO 2006086669 A8 WO2006086669 A8 WO 2006086669A8 US 2006004801 W US2006004801 W US 2006004801W WO 2006086669 A8 WO2006086669 A8 WO 2006086669A8
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
aptamer
regulated nucleic
ligand
aptamer regulated
Prior art date
Application number
PCT/US2006/004801
Other languages
French (fr)
Other versions
WO2006086669A2 (en
WO2006086669A3 (en
Inventor
Christina D Smolke
Travis S Bayer
Original Assignee
California Inst Of Techn
Christina D Smolke
Travis S Bayer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/243,889 external-priority patent/US9315862B2/en
Application filed by California Inst Of Techn, Christina D Smolke, Travis S Bayer filed Critical California Inst Of Techn
Priority to US11/884,110 priority Critical patent/US8772464B2/en
Publication of WO2006086669A2 publication Critical patent/WO2006086669A2/en
Publication of WO2006086669A8 publication Critical patent/WO2006086669A8/en
Publication of WO2006086669A3 publication Critical patent/WO2006086669A3/en
Priority to US14/287,824 priority patent/US9309568B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes

Abstract

The invention relates to aptamer-regulated, ligand-responsive nucleic acids, or 'ampliSwitches,' and uses thereof. Particular embodiments include a ligand- responsive nucleic acid that comprises a primer sequence domain and an aptamer domain that is responsive to a ligand.
PCT/US2006/004801 2004-10-05 2006-02-09 Aptamer regulated nucleic acids and uses thereof WO2006086669A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/884,110 US8772464B2 (en) 2004-10-05 2006-02-09 Aptamer regulated nucleic acids and uses thereof
US14/287,824 US9309568B2 (en) 2004-10-05 2014-05-27 Aptamer regulated nucleic acids and uses thereof

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US65142405P 2005-02-09 2005-02-09
US60/651,424 2005-02-09
US11/243,889 US9315862B2 (en) 2004-10-05 2005-10-05 Aptamer regulated nucleic acids and uses thereof
US11/243,889 2005-10-05
PCT/US2005/036161 WO2006042112A2 (en) 2004-10-05 2005-10-05 Aptamer regulated nucleic acids and uses thereof
USPCT/US2005/036161 2005-10-05

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/036161 Continuation-In-Part WO2006042112A2 (en) 2004-10-05 2005-10-05 Aptamer regulated nucleic acids and uses thereof

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US11/884,110 A-371-Of-International US8772464B2 (en) 2004-10-05 2006-02-09 Aptamer regulated nucleic acids and uses thereof
US14/287,824 Division US9309568B2 (en) 2004-10-05 2014-05-27 Aptamer regulated nucleic acids and uses thereof

Publications (3)

Publication Number Publication Date
WO2006086669A2 WO2006086669A2 (en) 2006-08-17
WO2006086669A8 true WO2006086669A8 (en) 2006-12-07
WO2006086669A3 WO2006086669A3 (en) 2007-03-01

Family

ID=36580287

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/004801 WO2006086669A2 (en) 2004-10-05 2006-02-09 Aptamer regulated nucleic acids and uses thereof

Country Status (1)

Country Link
WO (1) WO2006086669A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006042112A2 (en) 2004-10-05 2006-04-20 California Institute Of Technology Aptamer regulated nucleic acids and uses thereof
WO2008058291A2 (en) 2006-11-09 2008-05-15 California Institute Of Technology Modular aptamer-regulated ribozymes
WO2009011855A2 (en) * 2007-07-16 2009-01-22 California Institute Of Technology Selection of nucleic acid-based sensor domains within nucleic acid switch platform
US20120165387A1 (en) 2007-08-28 2012-06-28 Smolke Christina D General composition framework for ligand-controlled RNA regulatory systems
US8865667B2 (en) 2007-09-12 2014-10-21 California Institute Of Technology Higher-order cellular information processing devices
US9029524B2 (en) 2007-12-10 2015-05-12 California Institute Of Technology Signal activated RNA interference
US9145555B2 (en) 2009-04-02 2015-09-29 California Institute Of Technology Integrated—ligand-responsive microRNAs
US9310357B2 (en) * 2012-09-28 2016-04-12 Src, Inc. Detection of chemical and biological agents using oligonucleotide aptamers
WO2015045593A1 (en) 2013-09-30 2015-04-02 Ｎｅｃソリューションイノベータ株式会社 Method and kit for analyzing target
WO2017214202A1 (en) * 2016-06-07 2017-12-14 Cepheid Thermostable polymerase inhibitor compositions and methods
US11359240B2 (en) 2017-03-15 2022-06-14 National University Corporation Gunma University Method for detecting target molecule in which rolling circle amplification is used
TW201930593A (en) * 2017-09-11 2019-08-01 英商卡美納生物科學公司 Compositions and methods for complex unbiased solution profiling
GB2570157A (en) * 2018-01-15 2019-07-17 Dnanudge Ltd Molecule detection
US10467679B1 (en) 2019-04-15 2019-11-05 Dnanudge Limited Product recommendation device and method
US10811140B2 (en) 2019-03-19 2020-10-20 Dnanudge Limited Secure set-up of genetic related user account
US10699806B1 (en) 2019-04-15 2020-06-30 Dnanudge Limited Monitoring system, wearable monitoring device and method
CN114990126B (en) * 2022-08-03 2022-11-18 珠海宝锐生物科技有限公司 Aptamer specifically binding to Bst DNA polymerase large-fragment active site and application thereof

Also Published As

Publication number Publication date
WO2006086669A2 (en) 2006-08-17
WO2006086669A3 (en) 2007-03-01

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