JP5420837B2 - 胚幹細胞の培地及び培養 - Google Patents

胚幹細胞の培地及び培養 Download PDF

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JP5420837B2
JP5420837B2 JP2007531300A JP2007531300A JP5420837B2 JP 5420837 B2 JP5420837 B2 JP 5420837B2 JP 2007531300 A JP2007531300 A JP 2007531300A JP 2007531300 A JP2007531300 A JP 2007531300A JP 5420837 B2 JP5420837 B2 JP 5420837B2
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stem cells
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JP2008512122A (ja
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ジェイムズ エイ トムソン
テナイル ルードウィッグ
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ウィスコンシン アラムニ リサーチ ファンデーション
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JP2007531300A 2004-09-08 2005-09-08 胚幹細胞の培地及び培養 Expired - Lifetime JP5420837B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US60804004P 2004-09-08 2004-09-08
US60/608,040 2004-09-08
PCT/US2005/031838 WO2006029197A1 (en) 2004-09-08 2005-09-08 Medium and culture of embryonic stem cells

Related Child Applications (2)

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JP2011165510A Division JP2011206064A (ja) 2004-09-08 2011-07-28 胚幹細胞の培地及び培養
JP2013202449A Division JP2014014373A (ja) 2004-09-08 2013-09-27 胚幹細胞の培地及び培養

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JP2008512122A JP2008512122A (ja) 2008-04-24
JP2008512122A5 JP2008512122A5 (https=) 2013-11-21
JP5420837B2 true JP5420837B2 (ja) 2014-02-19

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JP2007531300A Expired - Lifetime JP5420837B2 (ja) 2004-09-08 2005-09-08 胚幹細胞の培地及び培養
JP2011165510A Pending JP2011206064A (ja) 2004-09-08 2011-07-28 胚幹細胞の培地及び培養
JP2013202449A Pending JP2014014373A (ja) 2004-09-08 2013-09-27 胚幹細胞の培地及び培養

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JP2011165510A Pending JP2011206064A (ja) 2004-09-08 2011-07-28 胚幹細胞の培地及び培養
JP2013202449A Pending JP2014014373A (ja) 2004-09-08 2013-09-27 胚幹細胞の培地及び培養

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US (5) US7449334B2 (https=)
EP (1) EP1791948B1 (https=)
JP (3) JP5420837B2 (https=)
KR (1) KR101264940B1 (https=)
CN (2) CN101052712A (https=)
AT (1) ATE549395T1 (https=)
AU (1) AU2005282510B2 (https=)
BR (1) BRPI0514778B1 (https=)
CA (1) CA2579643C (https=)
ES (1) ES2383813T3 (https=)
GB (1) GB2432846B (https=)
IL (1) IL181437A (https=)
IS (1) IS8618A (https=)
MX (1) MX2007002390A (https=)
NO (1) NO344695B1 (https=)
NZ (1) NZ553241A (https=)
WO (1) WO2006029197A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200034331A (ko) * 2018-09-21 2020-03-31 메디포스트(주) 피부 세포 활성화용 조성물 및 이의 용도

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005282414C1 (en) * 2004-09-08 2011-04-07 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells
CN101052712A (zh) * 2004-09-08 2007-10-10 威斯康星校友研究基金会 培养人胚胎干细胞
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
CA2613889A1 (en) 2005-06-08 2006-12-14 Centocor, Inc. A cellular therapy for ocular degeneration
US20070025973A1 (en) * 2005-07-29 2007-02-01 Fitzsimmons Frederick J Reprogramming of adult or neonic stem cells and methods of use
EP1945758A2 (en) * 2005-10-07 2008-07-23 Cellartis AB A method for obtaining a xeno-free hbs cell line
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
GB0613756D0 (en) * 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
WO2008089351A1 (en) * 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells
WO2008112323A1 (en) * 2007-03-15 2008-09-18 California Stem Cell, Inc. Cardiomyocytes and methods of producing and purifying cardiomyocytes
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen
US8815585B2 (en) * 2007-06-29 2014-08-26 Cellular Dynamics International, Inc. Automated method and apparatus for embryonic stem cell culture
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CA3114827C (en) 2007-07-31 2023-09-05 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
EP2229434B1 (en) 2007-11-27 2011-09-07 Lifescan, Inc. Differentiation of human embryonic stem cells
EP2233565B1 (en) * 2007-12-28 2015-05-13 Fujirebio Inc. Medium for mammalian somatic cells and additive therefor
KR101731474B1 (ko) 2008-02-21 2017-05-11 얀센 바이오테크 인코포레이티드 세포 부착, 배양 및 탈리를 위한 방법, 표면 개질 플레이트 및 조성물
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US8513012B2 (en) * 2008-05-02 2013-08-20 Cellular Dynamics International, Inc. Method for production of mast cells from stem cells
PL2310492T3 (pl) 2008-06-30 2015-12-31 Janssen Biotech Inc Różnocowanie pluripotencjalnych komórek macierzystych
EP2318516A1 (en) * 2008-06-30 2011-05-11 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
US10047346B2 (en) 2008-08-08 2018-08-14 Mayo Foundation For Medical Education And Research Method of treating heart tissue using induced pluripotent stem cells
MX349178B (es) 2008-10-31 2017-07-17 Centocor Ortho Biotech Inc Diferenciación de células madre embrionarias humanas al linaje endocrino pancreático.
PL2346988T3 (pl) 2008-10-31 2017-10-31 Janssen Biotech Inc Różnicowanie ludzkich zarodkowych komórek macierzystych do linii endokrynnej trzustki
ES2642070T3 (es) 2008-11-20 2017-11-15 Janssen Biotech, Inc. Cultivo de células madre pluripotentes en microportadores
ES2584053T3 (es) 2008-11-20 2016-09-23 Janssen Biotech, Inc. Métodos y composiciones para la unión de células y cultivo en sustratos planos
EP2398897B1 (en) * 2009-02-20 2017-06-28 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
KR101720961B1 (ko) 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
SG177483A1 (en) 2009-07-20 2012-02-28 Janssen Biotech Inc Differentiation of human embryonic stem cells
EP2456859A4 (en) 2009-07-20 2015-03-18 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
KR102058901B1 (ko) 2009-07-20 2019-12-24 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
ES2665006T3 (es) * 2009-10-29 2018-04-24 Janssen Biotech, Inc. Células madre pluripotentes
US10485829B2 (en) 2009-11-17 2019-11-26 Astellas Institute For Regenerative Medicine Methods of producing human RPE cells and pharmaceutical preparations of human RPE cells
FI20096288A0 (fi) 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
SG181687A1 (en) 2009-12-23 2012-07-30 Centocor Ortho Biotech Inc Differentiation of human embryonic stem cells
CA2784425A1 (en) 2009-12-23 2011-06-30 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
EP2542667A4 (en) 2010-03-01 2013-07-31 Janssen Biotech Inc PROCESS FOR CLEANING CELLS OBTAINED FROM PLURIPOTENTIAL STEM CELLS
RU2663339C1 (ru) 2010-05-12 2018-08-03 Янссен Байотек, Инк. Дифференцирование эмбриональных стволовых клеток человека
US9273286B2 (en) 2010-06-13 2016-03-01 Institute Of Biophysics, Chinese Academy Of Sciences Methods and compositions for preparing cardiomyocytes from stem cells and uses thereof
EP2596119B8 (en) 2010-07-23 2021-06-02 Astellas Institute for Regenerative Medicine Methods for detection of rare subpopulations of cells and highly purified compositions of cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
EP2601288B1 (en) 2010-08-05 2016-04-06 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
CN103221536B (zh) 2010-08-31 2016-08-31 詹森生物科技公司 人胚胎干细胞的分化
ES2658146T3 (es) 2010-08-31 2018-03-08 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas
EP2611907B1 (en) 2010-08-31 2016-05-04 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
CN102559587A (zh) * 2010-12-16 2012-07-11 中国科学院上海药物研究所 诱导性多能干细胞的制备方法以及用于制备诱导性多能干细胞的培养基
CN103384680B (zh) * 2010-12-17 2015-08-26 拜奥拉米那公司 细胞培养基
US9133266B2 (en) * 2011-05-06 2015-09-15 Wisconsin Alumni Research Foundation Vitronectin-derived cell culture substrate and uses thereof
US20130029416A1 (en) 2011-07-22 2013-01-31 Tayaramma Thatava Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny
WO2013022584A1 (en) 2011-08-05 2013-02-14 Corning Incorporated Modulation of the mammalian receptor mtor to inhibit stem cell differentiation into neurons
CN102399742B (zh) * 2011-09-30 2014-07-30 东莞中山大学研究院 用于哺乳动物唾液腺上皮细胞和唾液腺干细胞培养的无血清培养液
WO2013082268A1 (en) 2011-11-30 2013-06-06 The Wistar Institute Of Anatomy And Biology Methods and compositions for regulation of cell aging, carcinogenesis, and reprogramming
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
KR102320571B1 (ko) 2011-12-05 2021-11-02 팩터 바이오사이언스 인크. 세포를 형질감염시키는 방법들 및 생성물들
US8772460B2 (en) 2011-12-16 2014-07-08 Wisconsin Alumni Research Foundation Thermostable FGF-2 mutant having enhanced stability
KR102203056B1 (ko) 2011-12-22 2021-01-14 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 단일 인슐린 호르몬 양성 세포로의 분화
HK1206058A1 (en) 2012-03-07 2015-12-31 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
EP2841563B1 (en) 2012-04-24 2019-06-12 Dan S. Kaufman Method for developing natural killer cells from stem cells
AU2013271581B2 (en) 2012-06-08 2018-08-09 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
CN104508121B (zh) 2012-07-23 2018-01-19 中国科学院生物物理研究所 体外诱导多能干细胞分化为心室肌细胞的方法
WO2014071219A1 (en) 2012-11-01 2014-05-08 Factor Bioscience Inc. Methods and products for expressing proteins in cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
BR112015015770A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc cultivo de células-tronco embrionárias humanas na interface ar-líquido para diferenciação em células pancreáticas endócrinas
JP6557147B2 (ja) 2012-12-31 2019-08-07 ヤンセン バイオテツク，インコーポレーテツド Ｈｂ９調節物を用いたヒト胚性幹細胞の膵臓内分泌細胞への分化
JP6677631B2 (ja) 2013-03-13 2020-04-08 ウイスコンシン アラムナイ リサーチ ファウンデーシヨンＷｉｓｃｏｎｓｉｎ Ａｌｕｍｎｉ Ｒｅｓｅａｒｃｈ Ｆｏｕｎｄａｔｉｏｎ 定義された条件下におけるヒト多能性幹細胞の造血内皮分化のための方法及び材料
NL2011170C2 (en) * 2013-07-15 2015-01-21 Univ Erasmus Medical Ct Method and culture medium for in vitro culturing of stem cells.
WO2015042356A1 (en) 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
ES2939542T3 (es) 2014-01-31 2023-04-24 Factor Bioscience Inc Métodos y productos para la producción y la administración de ácido nucleico
EP3134515B1 (en) 2014-04-24 2019-03-27 Board of Regents, The University of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
US10280400B2 (en) 2014-05-05 2019-05-07 Indiana University Research And Technology Corporation Method for producing retinal ganglion cells from pluripotent cells
US10006006B2 (en) 2014-05-16 2018-06-26 Janssen Biotech, Inc. Use of small molecules to enhance MAFA expression in pancreatic endocrine cells
JP7199809B2 (ja) 2015-02-13 2023-01-06 ファクター バイオサイエンス インコーポレイテッド 核酸製品及びその投与方法
CN104830772A (zh) * 2015-05-28 2015-08-12 深圳富利鑫健康产业发展有限公司 一种造血干细胞培养基及其应用和干细胞培养方法
CN108431211B (zh) * 2015-10-20 2026-03-24 富士胶片细胞动力公司 通过遗传编程的多谱系造血前体细胞产生
DK3443073T3 (da) 2016-04-14 2024-09-02 Janssen Biotech Inc Differentiering af pluripotente stamceller til intestinale mellemtarmendodermceller
US10576167B2 (en) 2016-08-17 2020-03-03 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
EP3518941B1 (en) 2016-09-30 2022-03-09 Mayo Foundation for Medical Education and Research Viral vectors for nuclear reprogramming
SG11201906731XA (en) 2017-01-23 2019-08-27 Stemcell Technologies Canada Inc Media and methods for enhancing the survival and proliferation of stem cells
WO2018195175A1 (en) 2017-04-18 2018-10-25 FUJIFILM Cellular Dynamics, Inc. Antigen-specific immune effector cells
CA3064864A1 (en) 2017-05-25 2018-11-29 New York Stem Cell Foundation, Inc. Method and composition for generating basal forebrain cholinergic neurons (bfcns)
CN110799639A (zh) * 2017-06-27 2020-02-14 味之素株式会社 含有核黄素衍生物的培养基
KR102121042B1 (ko) * 2017-12-06 2020-06-09 이정복 조류의 에그로부터 분리한 세포인 배반엽 전분화능세포, 신경줄기세포, 배아섬유아세포 컨디션드 배지를 유효성분으로 하는 세포 증식, 피부재생 및 주름 개선용 배지 조성물
WO2019177420A1 (ko) * 2018-03-16 2019-09-19 사회복지법인 삼성생명공익재단 Fgf-17을 유효 성분으로 포함하는 줄기세포 증식 촉진용 배지 조성물 및 이를 이용한 줄기세포 증식 촉진 방법
JP7699552B2 (ja) 2019-07-03 2025-06-27 ファクター バイオサイエンス インコーポレイテッド カチオン性脂質及びその使用
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
CN111690595B (zh) * 2020-07-21 2021-07-16 生物岛实验室 一种无血清无饲养层的胚胎干细胞或多能干细胞培养基及其应用
CN112029708A (zh) * 2020-09-09 2020-12-04 江苏育瑞康生物科技有限公司 一种干细胞培养系统

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
ES2705683T3 (es) * 2002-12-16 2019-03-26 Technion Res & Dev Foundation Medio de cultivo de células madre pluripotentes
KR100632851B1 (ko) 2004-01-20 2006-10-13 한국원자력연구소 전분화능 줄기세포의 분화를 억제하는 방법
KR20070029745A (ko) * 2004-05-21 2007-03-14 위셀 리서치 인스티튜트, 인크. 배아 줄기 세포의 영양 세포 독립적 장기간 배양
AU2005282414C1 (en) * 2004-09-08 2011-04-07 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells
CN101052712A (zh) * 2004-09-08 2007-10-10 威斯康星校友研究基金会 培养人胚胎干细胞

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200034331A (ko) * 2018-09-21 2020-03-31 메디포스트(주) 피부 세포 활성화용 조성물 및 이의 용도
KR102216302B1 (ko) 2018-09-21 2021-02-17 메디포스트(주) 피부 세포 활성화용 조성물 및 이의 용도

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