JP5083325B2 - 走査型レーザ顕微鏡 - Google Patents
走査型レーザ顕微鏡 Download PDFInfo
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- JP5083325B2 JP5083325B2 JP2009545455A JP2009545455A JP5083325B2 JP 5083325 B2 JP5083325 B2 JP 5083325B2 JP 2009545455 A JP2009545455 A JP 2009545455A JP 2009545455 A JP2009545455 A JP 2009545455A JP 5083325 B2 JP5083325 B2 JP 5083325B2
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
- G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
- G02B26/10—Scanning systems
- G02B26/105—Scanning systems with one or more pivoting mirrors or galvano-mirrors
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- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Computer Vision & Pattern Recognition (AREA)
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Description
Claims (3)
- レーザ光を試料上で走査し、該試料からの光を用いて前記試料の画像を取得する走査型レーザ顕微鏡において、
前記レーザ光を1ラインずつスキャンさせる走査手段と、
設定されたフレームレートから求められる1フレームの走査に必要な時間に基づいて、1ラインの走査に用いられる時間として要求される要求ライン走査時間を算出する算出手段と、
前記走査手段を駆動する駆動波形データの1ライン分のデータ数、および、前記駆動波形データの読み出し周期のうちの少なくとも一方を調整して、前記走査手段が1ライン走査する時間を、前記算出手段により算出された要求ライン走査時間に略一致させる調整手段と
を備えることを特徴とする走査型レーザ顕微鏡。 - 前記走査型レーザ顕微鏡では、前記レーザ光がスキャンされるスキャン領域のうちの一部のキャプチャ領域の画像が取得され、
前記調整手段は、前記駆動波形データの1ライン分のデータ数のうちの、前記キャプチャ領域以外のスキャン領域についての駆動波形データのデータ数、または、所定のラインの終端から次のラインの先端に前記走査手段を駆動する間の駆動波形データのデータ数を調整する
ことを特徴とする請求項1に記載の走査型レーザ顕微鏡。 - 基準周波数のクロック信号を所定の分周比で変換し、前記読み出し周期の信号を生成する生成手段をさらに備え、
前記調整手段は、前記生成手段における分周比を変更して前記読み出し周期を段階的に短縮させ、前記走査手段が1ライン走査する時間が、前記要求ライン走査時間とならなかった場合、前記駆動波形データのデータ数を増減させて、前記走査手段が1ライン走査する時間を前記要求ライン走査時間に略一致させる
ことを特徴とする請求項1に記載の走査型レーザ顕微鏡。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009545455A JP5083325B2 (ja) | 2007-12-13 | 2008-12-12 | 走査型レーザ顕微鏡 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007322064 | 2007-12-13 | ||
JP2007322064 | 2007-12-13 | ||
PCT/JP2008/072611 WO2009075346A1 (ja) | 2007-12-13 | 2008-12-12 | 走査型レーザ顕微鏡 |
JP2009545455A JP5083325B2 (ja) | 2007-12-13 | 2008-12-12 | 走査型レーザ顕微鏡 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2009075346A1 JPWO2009075346A1 (ja) | 2011-04-28 |
JP5083325B2 true JP5083325B2 (ja) | 2012-11-28 |
Family
ID=40755582
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009545455A Active JP5083325B2 (ja) | 2007-12-13 | 2008-12-12 | 走査型レーザ顕微鏡 |
Country Status (4)
Country | Link |
---|---|
US (1) | US8194311B2 (ja) |
EP (1) | EP2237097A4 (ja) |
JP (1) | JP5083325B2 (ja) |
WO (1) | WO2009075346A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5554970B2 (ja) * | 2009-11-25 | 2014-07-23 | オリンパス株式会社 | 走査型顕微鏡 |
US10027855B2 (en) | 2010-06-30 | 2018-07-17 | Ge Healthcare Bio-Science Corp. | System for synchronization in a line scanning imaging microscope |
CN103698880B (zh) * | 2013-12-26 | 2016-01-27 | 中国科学院苏州生物医学工程技术研究所 | 激光扫描共聚焦显微镜用二维针孔电动实时调整系统 |
WO2016123479A1 (en) | 2015-01-31 | 2016-08-04 | Board Of Regents, The University Of Texas System | High-speed laser scanning microscopy platform for high-throughput automated 3d imaging and functional volumetric imaging |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003177323A (ja) * | 2001-12-11 | 2003-06-27 | Olympus Optical Co Ltd | 走査型レーザ顕微鏡、駆動波形データ生成方法、及びその制御プログラム |
JP2005352030A (ja) * | 2004-06-09 | 2005-12-22 | Olympus Corp | 走査型レーザ観察装置、観察方法及び観察プログラム |
JP2006145857A (ja) * | 2004-11-19 | 2006-06-08 | Olympus Corp | 走査型レーザ顕微鏡 |
JP2006195440A (ja) * | 2004-12-13 | 2006-07-27 | Olympus Corp | 走査型レーザ顕微鏡装置 |
JP2006227600A (ja) * | 2005-01-18 | 2006-08-31 | Olympus Corp | 走査型顕微鏡および標本画像取得方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003098468A (ja) | 2001-09-20 | 2003-04-03 | Nikon Corp | スキャナ装置およびそれを備えたコンフォーカル顕微鏡 |
EP1793256A4 (en) * | 2004-06-14 | 2010-05-26 | Olympus Corp | OPTICAL GRID MICROSCOPE OBSERVATION DEVICE |
DE102004034951A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Verfahren zur bildlichen Erfassung von Objekten mittels eines Lichtrastermikrokopes mit linienförmiger Abtastung |
JP2006119347A (ja) * | 2004-10-21 | 2006-05-11 | Olympus Corp | レーザ走査型顕微鏡 |
EP1669789B1 (en) * | 2004-12-13 | 2011-07-06 | Olympus Corporation | Laser scanning microscope apparatus |
US7612350B2 (en) * | 2005-01-18 | 2009-11-03 | Olympus Corporation | Scanning microscope and specimen image obtaining method in which adjacent data obtaining points are not consecutively obtained |
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2008
- 2008-12-12 WO PCT/JP2008/072611 patent/WO2009075346A1/ja active Application Filing
- 2008-12-12 EP EP08858605A patent/EP2237097A4/en not_active Withdrawn
- 2008-12-12 JP JP2009545455A patent/JP5083325B2/ja active Active
-
2010
- 2010-06-11 US US12/814,166 patent/US8194311B2/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003177323A (ja) * | 2001-12-11 | 2003-06-27 | Olympus Optical Co Ltd | 走査型レーザ顕微鏡、駆動波形データ生成方法、及びその制御プログラム |
JP2005352030A (ja) * | 2004-06-09 | 2005-12-22 | Olympus Corp | 走査型レーザ観察装置、観察方法及び観察プログラム |
JP2006145857A (ja) * | 2004-11-19 | 2006-06-08 | Olympus Corp | 走査型レーザ顕微鏡 |
JP2006195440A (ja) * | 2004-12-13 | 2006-07-27 | Olympus Corp | 走査型レーザ顕微鏡装置 |
JP2006227600A (ja) * | 2005-01-18 | 2006-08-31 | Olympus Corp | 走査型顕微鏡および標本画像取得方法 |
Also Published As
Publication number | Publication date |
---|---|
JPWO2009075346A1 (ja) | 2011-04-28 |
EP2237097A4 (en) | 2013-02-27 |
WO2009075346A1 (ja) | 2009-06-18 |
US8194311B2 (en) | 2012-06-05 |
EP2237097A1 (en) | 2010-10-06 |
US20100245994A1 (en) | 2010-09-30 |
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