JP4826995B2 - 抗体の変質を防止する精製方法 - Google Patents

抗体の変質を防止する精製方法 Download PDF

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Publication number
JP4826995B2
JP4826995B2 JP2005013897A JP2005013897A JP4826995B2 JP 4826995 B2 JP4826995 B2 JP 4826995B2 JP 2005013897 A JP2005013897 A JP 2005013897A JP 2005013897 A JP2005013897 A JP 2005013897A JP 4826995 B2 JP4826995 B2 JP 4826995B2
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antibody
arginine
protein
buffer
recovered
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JP2005206602A (ja
JP2005206602A5 (https=
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良輔 弓岡
大輔 江島
力 荒川
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Ajinomoto Co Inc
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Ajinomoto Co Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2839—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
    • C07K16/2845—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta2-subunit-containing molecules, e.g. CD11, CD18
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2005013897A 2004-01-21 2005-01-21 抗体の変質を防止する精製方法 Expired - Lifetime JP4826995B2 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US53750304P 2004-01-21 2004-01-21
US60/537503 2004-01-21
US63140704P 2004-11-30 2004-11-30
US60/631407 2004-11-30

Publications (3)

Publication Number Publication Date
JP2005206602A JP2005206602A (ja) 2005-08-04
JP2005206602A5 JP2005206602A5 (https=) 2008-03-06
JP4826995B2 true JP4826995B2 (ja) 2011-11-30

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ID=34753090

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JP2005013897A Expired - Lifetime JP4826995B2 (ja) 2004-01-21 2005-01-21 抗体の変質を防止する精製方法

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US (3) US8084032B2 (https=)
EP (1) EP1568710B1 (https=)
JP (1) JP4826995B2 (https=)
CN (1) CN1680426B (https=)
DE (1) DE602005018406D1 (https=)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
DE602006003108D1 (de) * 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
ATE551433T1 (de) * 2005-05-06 2012-04-15 Gen Probe Inc Verfahren und produkte zum erfassen von nukleinsäurezielen
US20080125580A1 (en) 2006-07-14 2008-05-29 Genentech, Inc. Refolding of Recombinant Proteins
WO2008031020A2 (en) 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography
TWI456062B (zh) 2006-09-13 2014-10-11 Abbvie Inc 細胞培養改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
EP2089429B1 (en) * 2006-10-19 2014-11-26 Janssen Biotech, Inc. Process for preparing unaggregated antibody fc domains
JP5505853B2 (ja) 2007-02-13 2014-05-28 味の素株式会社 微酸性アルギニンを添加剤とするウイルス不活化法
CN102089319B (zh) * 2008-05-08 2013-11-13 味之素株式会社 蛋白质的重折叠方法
JP4542172B2 (ja) * 2008-05-19 2010-09-08 日本電波工業株式会社 感知装置及び感知方法
EP2321338A1 (en) * 2008-08-14 2011-05-18 Merck Sharp & Dohme Corp. Methods for purifying antibodies using protein a affinity chromatography
AU2009347206C1 (en) 2008-10-20 2016-12-08 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
MX2011004201A (es) 2008-10-20 2011-05-24 Abbott Lab Desactivacion viral durante purificacion de anticuerpos.
JP2011041475A (ja) * 2009-08-19 2011-03-03 Asahi Kasei Medical Co Ltd 抗体製造方法
EP2513134B1 (en) * 2009-12-18 2017-09-06 Novartis AG Wash solution and method for affinity chromatography
SG182553A1 (en) * 2010-01-22 2012-08-30 Boehringer Ingelheim Int Chromatographic method for purifying fc-containing proteins
EP2538790B1 (en) 2010-02-26 2015-07-29 Ajinomoto Co., Inc. Virus-inactivating composition containing low-molecular weight compound and arginine
EP3266793A1 (en) * 2010-06-21 2018-01-10 Kyowa Hakko Kirin Co., Ltd. Method for purifying protein using amino acid
BR112013030628B1 (pt) * 2011-06-01 2022-01-25 Novartis Ag Método para produção de uma proteína purificada de interesse utilizando uma matriz de cromatografia de afinidade, e método para produção de um anticorpo purificado, fragmento de anticorpo ou proteína de fusão de fc utilizando uma coluna de proteína a
CN107365388B (zh) 2011-06-23 2021-05-14 味之素株式会社 多聚体蛋白的制备方法
US20150239956A1 (en) * 2012-06-27 2015-08-27 Asahi Kasei Medical Co., Ltd. High-affinity antibody and method for manufacturing the same
WO2014054744A1 (ja) * 2012-10-03 2014-04-10 協和発酵キリン株式会社 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法
BR112015031541A2 (pt) 2013-06-17 2017-07-25 De Staat Der Nederlanden Vert Door De Mini Van Vws Miniie Van Volksgezondheid Welzijn En Sport métodos para o impedimento da agregação de componentes virais
JP6236948B2 (ja) * 2013-07-17 2017-11-29 東ソー株式会社 抗体精製用溶出液および当該溶出液を用いた抗体精製方法
CN105273041A (zh) * 2014-07-08 2016-01-27 上海中信国健药业股份有限公司 一种蛋白质亲和层析洗脱方法
TW201628649A (zh) 2014-10-09 2016-08-16 再生元醫藥公司 減少醫藥調配物中微可見顆粒之方法
AU2015335743B2 (en) 2014-10-23 2020-12-24 Amgen Inc. Reducing viscosity of pharmaceutical formulations
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
EP3374371A4 (en) * 2015-11-09 2019-12-11 University of Notre Dame SMALL MOLECULE AFFINITY MEMBRANE PURIFICATION SYSTEMS AND USE THEREOF
HRP20160086A2 (hr) 2016-01-27 2017-08-11 Sveučilište u Zagrebu Eluens za imunoafinitetnu kromatografiju virusa i proteina
US11186858B1 (en) 2016-03-15 2021-11-30 Fresenius Kabi Deutschland Gmbh Methods for increasing biosimilarity
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US11708390B2 (en) 2016-05-11 2023-07-25 Cytiva Bioprocess R&D Ab Method of storing a separation matrix
CN109071613A (zh) 2016-05-11 2018-12-21 通用电气医疗集团生物工艺研发股份公司 分离基质
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
JP6987424B2 (ja) 2016-05-11 2022-01-05 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを洗浄および／または消毒する方法
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10688412B2 (en) 2016-07-25 2020-06-23 Cehpalon, Inc. Affinity chromatography wash buffer
KR102369014B1 (ko) 2016-08-16 2022-03-02 리제너론 파아마슈티컬스, 인크. 혼합물로부터 개별 항체들을 정량하는 방법
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
EA201990986A1 (ru) 2016-10-25 2019-09-30 Ридженерон Фармасьютикалз, Инк. Способы и системы анализа хроматографических данных
JP7377596B2 (ja) 2017-02-22 2023-11-10 アムジエン・インコーポレーテツド 低粘度、高濃度エボロクマブ製剤及びそれらの製造方法
US20200003764A1 (en) * 2017-04-07 2020-01-02 Konica Minolta, Inc. Method for manufacturing purified product of protein-modified phosphor-integrated particle, method for manufacturing fluorescent staining liquid, purified product of protein-modified phosphor-integrated particle, and filter for purifying fluorescent staining liquid and protein-modified phosphor-integrated particle
WO2019060062A1 (en) 2017-09-19 2019-03-28 Regeneron Pharmaceuticals Inc. METHODS OF REDUCING PARTICLE FORMATION AND COMPOSITIONS FORMED THEREFROM
JP7158015B2 (ja) * 2017-11-09 2022-10-21 国立研究開発法人産業技術総合研究所 ポリペプチドの凝集化抑制方法
CN111902720B (zh) 2018-03-21 2025-02-07 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
TWI853823B (zh) 2018-07-02 2024-09-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
EA202192422A1 (ru) 2019-03-05 2021-12-09 Ридженерон Фармасьютикалз, Инк. Человеческий сывороточный альбумин в составах
BR112021023542A2 (pt) 2019-08-02 2022-03-22 UCB Biopharma SRL Métodos para purificar anticorpos
JP2022550837A (ja) * 2019-10-04 2022-12-05 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング プロテインａアフィニティークロマトグラフィーにおけるモノクローナル抗体の溶離
CN112898413A (zh) * 2021-04-07 2021-06-04 杭州奕安济世生物药业有限公司 采用亲和层析纯化减少抗体聚集的方法
CN113980092B (zh) * 2021-12-09 2024-05-14 上海药明生物技术有限公司 一种蛋白质亲和纯化方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1323567C (en) 1987-10-05 1993-10-26 Gene R. Nathans Method for purification of antibodies
JPH02273194A (ja) 1989-04-14 1990-11-07 Sumitomo Chem Co Ltd ヒトモノクローナル抗体の安定な精製法
JPH08503698A (ja) 1992-10-02 1996-04-23 ザ ゼネラル ホスピタル コーポレーション アミノ酸模倣物を溶出試薬として用いる親和精製方法
US6171586B1 (en) * 1997-06-13 2001-01-09 Genentech, Inc. Antibody formulation
DE60213248T2 (de) 2001-06-05 2006-11-30 Genetics Institute, LLC, Cambridge Verfahren zur reinigung hochanionischer proteine
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
DE602006003108D1 (de) 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
JP5505853B2 (ja) 2007-02-13 2014-05-28 味の素株式会社 微酸性アルギニンを添加剤とするウイルス不活化法
CN102089319B (zh) 2008-05-08 2013-11-13 味之素株式会社 蛋白质的重折叠方法

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Publication number Publication date
JP2005206602A (ja) 2005-08-04
US8470328B2 (en) 2013-06-25
US20120142901A1 (en) 2012-06-07
CN1680426B (zh) 2010-05-26
EP1568710A3 (en) 2005-10-05
US8435527B2 (en) 2013-05-07
EP1568710A2 (en) 2005-08-31
EP1568710B1 (en) 2009-12-23
US20120264918A1 (en) 2012-10-18
CN1680426A (zh) 2005-10-12
US8084032B2 (en) 2011-12-27
DE602005018406D1 (de) 2010-02-04
US20050176109A1 (en) 2005-08-11

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