JP4766750B2 - 位置特定可能なアレイによるリガーゼ検出反応を用いた核酸配列差異の検出 - Google Patents

位置特定可能なアレイによるリガーゼ検出反応を用いた核酸配列差異の検出 Download PDF

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JP4766750B2
JP4766750B2 JP2000606786A JP2000606786A JP4766750B2 JP 4766750 B2 JP4766750 B2 JP 4766750B2 JP 2000606786 A JP2000606786 A JP 2000606786A JP 2000606786 A JP2000606786 A JP 2000606786A JP 4766750 B2 JP4766750 B2 JP 4766750B2
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oligonucleotide probe
oligonucleotide
ligation
nucleotide sequence
target
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JP2003520570A (ja
JP2003520570A5 (https=
Inventor
フランシス バラニー
ノーマン ピー. ジェリー
ナンシー イー. ウィトウスキ
ジョセフ デイ
ロバート ピー. ハンマー
ジョージ バラニー
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University of Minnesota Twin Cities
Louisiana State University
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Louisiana State University
University of Minnesota System
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JP2000606786A 1999-03-19 2000-03-17 位置特定可能なアレイによるリガーゼ検出反応を用いた核酸配列差異の検出 Expired - Fee Related JP4766750B2 (ja)

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US12535799P 1999-03-19 1999-03-19
US60/125,357 1999-03-19
PCT/US2000/007006 WO2000056927A2 (en) 1999-03-19 2000-03-17 Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

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JP2011091716A Division JP2011200230A (ja) 1999-03-19 2011-04-18 位置特定可能なアレイによるリガーゼ検出反応を用いた核酸配列差異の検出

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JP2003520570A JP2003520570A (ja) 2003-07-08
JP2003520570A5 JP2003520570A5 (https=) 2010-09-30
JP4766750B2 true JP4766750B2 (ja) 2011-09-07

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JP2011091716A Withdrawn JP2011200230A (ja) 1999-03-19 2011-04-18 位置特定可能なアレイによるリガーゼ検出反応を用いた核酸配列差異の検出

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US (2) US6506594B1 (https=)
EP (1) EP1208223A2 (https=)
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Families Citing this family (178)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000033244A2 (en) * 1998-11-27 2000-06-08 Synaptics (Uk) Limited Position sensor
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20020119448A1 (en) 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
US7041445B2 (en) 1999-11-15 2006-05-09 Clontech Laboratories, Inc. Long oligonucleotide arrays
US6235483B1 (en) * 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
US6872552B2 (en) * 2000-02-29 2005-03-29 Burt D. Ensley Method of reconstituting nucleic acid molecules
WO2001079548A2 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
EP1164201A1 (en) * 2000-06-14 2001-12-19 Facultés Universitaires Notre-Dame de la Paix Reverse detection for identification and/or quantification of nucleotide target sequences on biochips
AU784561B2 (en) * 2000-06-23 2006-05-04 Yissum Research Development Company Of The Hebrew University Of Jerusalem Positively-charged peptide nucleic acid analogs with improved properties
EP1186671A3 (en) * 2000-09-05 2003-12-17 Agilent Technologies, Inc. (a Delaware corporation) Method for hybridization of arrays on siliceous surfaces
DE60131903T2 (de) * 2000-10-24 2008-11-27 The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto Direkte multiplex charakterisierung von genomischer dna
US7091034B2 (en) * 2000-12-15 2006-08-15 Burstein Technologies, Inc. Detection system for disk-based laboratory and improved optical bio-disc including same
JP3605039B2 (ja) * 2001-01-09 2004-12-22 株式会社日立製作所 核酸マイクロアレイ、その製造方法及びそれを用いた核酸検出方法
JP4189471B2 (ja) * 2001-01-25 2008-12-03 クラスターテクノロジー株式会社 アモルファスカーボン基材
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
JP2003294742A (ja) * 2002-03-29 2003-10-15 Inst Of Physical & Chemical Res ハイブリダイゼーション用基板及びその使用方法
EP1491888B1 (en) 2002-04-03 2008-09-17 Mitsubishi Rayon Co., Ltd. Microarray comprising gel having biosubstance fixed thereto as well as manufacture and use thereof
US20070275411A1 (en) 2006-05-25 2007-11-29 Mcgall Glenn H Silane mixtures
US7332273B2 (en) 2002-06-20 2008-02-19 Affymetrix, Inc. Antireflective coatings for high-resolution photolithographic synthesis of DNA arrays
US7038037B2 (en) * 2002-06-20 2006-05-02 Isis Pharmaceuticals, Inc. Method for sequential support-bound synthesis of conjugated oligomeric compounds
WO2004025258A2 (en) * 2002-09-10 2004-03-25 Sydney Kimmel Cancer Center Gene segregation and biological sample classification methods
US7070922B2 (en) * 2002-12-04 2006-07-04 International Business Machines Corporation Surface treatment
WO2004058052A2 (en) 2002-12-20 2004-07-15 Applera Corporation Genetic polymorphisms associated with myocardial infarction, methods of detection and uses thereof
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
CN103396933B (zh) 2003-02-26 2016-04-20 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
EP1604040B1 (en) * 2003-03-07 2010-10-13 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
WO2005001113A2 (en) * 2003-06-27 2005-01-06 Thomas Jefferson University Methods for detecting nucleic acid variations
US20050042363A1 (en) * 2003-08-18 2005-02-24 Kukhtin Alexander V. Method for fabrication of biochips with a macroporous polymer substrate
US20050266417A1 (en) * 2003-09-12 2005-12-01 Francis Barany Methods for identifying target nucleic acid molecules
WO2005056837A2 (en) 2003-11-26 2005-06-23 Applera Corporation Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof
DE10359173B4 (de) * 2003-12-17 2006-11-09 Robert Bosch Gmbh Messvorrichtung mit mehreren auf einem Substrat angeordneten potentiometrischen Elektrodenpaaren
KR100580642B1 (ko) * 2004-01-12 2006-05-16 삼성전자주식회사 표면에 활성화된 카르복실기를 갖는 기판을 이용하여 생물분자를 고체 기판상에 고밀도로 고정화하는 방법 및 그에 의하여 제조되는 마이크로어레이
CA2555704A1 (en) 2004-02-10 2005-08-25 Cornell Research Foundation, Inc. Method for detection of promoter methylation status
DE602005023710D1 (de) * 2004-02-20 2010-11-04 Japan Science & Tech Agency Dna-array zur analyse der dna-methylierung, konstruktionsverfahren dafür sowie verfahren zur analyse der dna-methylierung
KR100590550B1 (ko) * 2004-03-12 2006-06-19 삼성전자주식회사 동일한 표적핵산에 결합하는 프로브 폴리뉴클레오티드사이의 거리가 최대가 되도록 배열되어 있는마이크로어레이 및 그를 제조하는 방법
US7723126B2 (en) * 2004-03-24 2010-05-25 Wisconsin Alumni Research Foundation Plasma-enhanced functionalization of inorganic oxide surfaces
US7767220B2 (en) * 2004-04-23 2010-08-03 Boston Scientific Scimed, Inc. Implantable or insertable medical articles having covalently modified, biocompatible surfaces
US7820380B2 (en) 2004-05-07 2010-10-26 Celera Corporation Genetic polymorphisms associated with liver fibrosis
US7338763B2 (en) * 2004-06-02 2008-03-04 Eppendorf Array Technologies S.A. Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays
US7785843B2 (en) * 2004-06-23 2010-08-31 Sequenom, Inc. Target-specific compomers and methods of use
US20060009038A1 (en) * 2004-07-12 2006-01-12 International Business Machines Corporation Processing for overcoming extreme topography
US7642055B2 (en) 2004-09-21 2010-01-05 Applied Biosystems, Llc Two-color real-time/end-point quantitation of microRNAs (miRNAs)
JPWO2006064745A1 (ja) * 2004-12-17 2008-06-12 東洋紡績株式会社 塩基多型の同定方法
US20060199207A1 (en) * 2005-02-24 2006-09-07 Matysiak Stefan M Self-assembly of molecules using combinatorial hybridization
WO2006099365A2 (en) 2005-03-11 2006-09-21 Applera Corporation Genetic polymorphisms associated with coronary heart disease, methods of detection and uses thereof
US7494776B2 (en) * 2005-07-07 2009-02-24 Beckman Coulter, Inc. Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands
US7799530B2 (en) 2005-09-23 2010-09-21 Celera Corporation Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof
WO2007076128A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
US20070264130A1 (en) * 2006-01-27 2007-11-15 Phluid, Inc. Infusion Pumps and Methods for Use
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
WO2007116129A1 (en) * 2006-04-10 2007-10-18 Wallac Oy A universal labeling system
CN1896284B (zh) * 2006-06-30 2013-09-11 博奥生物有限公司 一种鉴别等位基因类型的方法
WO2008039998A2 (en) * 2006-09-28 2008-04-03 President And Fellows Of Harvard College Methods for sequencing dna
US7910303B2 (en) 2006-10-20 2011-03-22 Celera Corporation Genetic polymorphisms associated with venous thrombosis, methods of detection and uses thereof
US8029902B2 (en) * 2006-12-11 2011-10-04 Wisconsin Alumni Research Foundation Plasma-enhanced functionalization of substrate surfaces with quaternary ammonium and quaternary phosphonium groups
US20120115131A1 (en) 2007-05-31 2012-05-10 Yale University Genetic lesion associated with cancer
JP2009011247A (ja) * 2007-07-05 2009-01-22 Sumitomo Bakelite Co Ltd 遺伝子の検出方法
US8039212B2 (en) 2007-11-05 2011-10-18 Celera Corporation Genetic polymorphisms associated with liver fibrosis, methods of detection and uses thereof
US8852864B2 (en) * 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
US8986253B2 (en) * 2008-01-25 2015-03-24 Tandem Diabetes Care, Inc. Two chamber pumps and related methods
US20090221620A1 (en) 2008-02-20 2009-09-03 Celera Corporation Gentic polymorphisms associated with stroke, methods of detection and uses thereof
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
US20090287180A1 (en) * 2008-05-19 2009-11-19 Diperna Paul M Disposable pump reservoir and related methods
EP3093351B1 (en) 2008-07-09 2018-04-18 Celera Corporation Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof
US8408421B2 (en) * 2008-09-16 2013-04-02 Tandem Diabetes Care, Inc. Flow regulating stopcocks and related methods
CA2737461A1 (en) * 2008-09-19 2010-03-25 Tandem Diabetes Care, Inc. Solute concentration measurement device and related methods
EP2192409B1 (en) * 2008-11-26 2014-04-23 Corning Incorporated Label independent detection biosensor composition and methods thereof
CN102282155B (zh) 2008-12-02 2017-06-09 日本波涛生命科学公司 磷原子修饰的核酸的合成方法
KR20100089688A (ko) * 2009-02-04 2010-08-12 삼성전자주식회사 표적핵산의 서열을 분석하는 방법
WO2010101696A1 (en) 2009-02-06 2010-09-10 Yale University A snp marker of breast and ovarian cancer risk
SG174617A1 (en) 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
CN102625850B (zh) 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
CA2766210A1 (en) 2009-06-25 2010-12-29 Yale University Single nucleotide polymorphisms in brca1 and cancer risk
RU2612521C2 (ru) 2009-07-06 2017-03-09 Онтории, Инк. Новые пролекарства нуклеиновых кислот и способы их применения
US8926561B2 (en) 2009-07-30 2015-01-06 Tandem Diabetes Care, Inc. Infusion pump system with disposable cartridge having pressure venting and pressure feedback
US9175339B2 (en) 2009-10-29 2015-11-03 Ngk Insulators, Ltd. Method for detection of target nucleic acid
US20110269735A1 (en) 2010-04-19 2011-11-03 Celera Corporation Genetic polymorphisms associated with statin response and cardiovascular diseases, methods of detection and uses thereof
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
JP5868324B2 (ja) 2010-09-24 2016-02-24 株式会社Wave Life Sciences Japan 不斉補助基
US20120108651A1 (en) 2010-11-02 2012-05-03 Leiden University Medical Center (LUMC) Acting on Behalf of Academic Hospital Leiden (AZL) Genetic polymorphisms associated with venous thrombosis and statin response, methods of detection and uses thereof
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US20120190020A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US20140024590A1 (en) 2011-02-18 2014-01-23 Yale University KRAS-Variant And Endometriosis
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
WO2012129352A1 (en) 2011-03-21 2012-09-27 Yale University The kras variant and tumor biology
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
US8809238B2 (en) 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
CN103796657B (zh) 2011-07-19 2017-07-11 波涛生命科学有限公司 合成官能化核酸的方法
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
EP2607495A1 (en) * 2011-12-23 2013-06-26 Genomica S.A.U. Method for detection of KRAS mutations
WO2013119845A1 (en) 2012-02-07 2013-08-15 Vibrant Holdings, Llc Substrates, peptide arrays, and methods
US9045803B2 (en) 2012-02-29 2015-06-02 Abbott Molecular Inc. Hepatitis B virus typing and resistance assay
US9180242B2 (en) 2012-05-17 2015-11-10 Tandem Diabetes Care, Inc. Methods and devices for multiple fluid transfer
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
CN102719538A (zh) * 2012-06-07 2012-10-10 上海交通大学 非侵入性产前诊断高危遗传性耳聋的基因芯片及制备方法
CA2877236C (en) * 2012-06-20 2021-03-09 Toray Industries, Inc. Method for detecting nucleic acid and nucleic acid detection kit
CA2879066C (en) 2012-07-13 2019-08-13 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant
PL2872485T3 (pl) 2012-07-13 2021-05-31 Wave Life Sciences Ltd. Asymetryczna grupa pomocnicza
KR102213609B1 (ko) 2012-07-13 2021-02-08 웨이브 라이프 사이언시스 리미티드 키랄 제어
EP2875156A4 (en) 2012-07-19 2016-02-24 Ariosa Diagnostics Inc SEQUENTIAL LIGATION-BASED MULTIPLEX DETECTION OF GENETIC VARIANTS
JP6234463B2 (ja) 2012-09-10 2017-11-22 ジーンスカイ ダイアグノスティクス(スーツォウ) インコーポレーテッドGenesky Diagnostics (Suzhou) Inc. 核酸の多重分析の方法
US10006909B2 (en) * 2012-09-28 2018-06-26 Vibrant Holdings, Llc Methods, systems, and arrays for biomolecular analysis
WO2014052487A1 (en) 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
US10286376B2 (en) 2012-11-14 2019-05-14 Vibrant Holdings, Llc Substrates, systems, and methods for array synthesis and biomolecular analysis
US9173998B2 (en) 2013-03-14 2015-11-03 Tandem Diabetes Care, Inc. System and method for detecting occlusions in an infusion pump
ES2746237T3 (es) 2013-03-14 2020-03-05 Univ Cornell Método para la cuantificación relativa de cambios en la metilación del ADN, usando reacciones combinadas de nucleasa, ligamiento, y polimerasa
WO2014200579A1 (en) 2013-06-13 2014-12-18 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
IL285521B2 (en) * 2013-08-19 2023-03-01 Singular Bio Inc Methods for single molecule detection
EP3041957A4 (en) 2013-09-04 2017-03-29 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
US10322173B2 (en) 2014-01-15 2019-06-18 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
JPWO2015108047A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤
JPWO2015108048A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
BR112016016400A2 (pt) 2014-01-16 2017-10-03 Wave Life Sciences Ltd Composições de oligonucleotídeos quiralmente controlados, seu uso, sua composição farmacêutica, e métodos
US10260111B1 (en) 2014-01-20 2019-04-16 Brett Eric Etchebarne Method of detecting sepsis-related microorganisms and detecting antibiotic-resistant sepsis-related microorganisms in a fluid sample
US10221447B2 (en) 2014-04-01 2019-03-05 Cornell University Detection of DNA methylation using combined nuclease ligation reactions
US10526601B2 (en) 2014-05-23 2020-01-07 Digenomix Corporation Haploidome determination by digitized transposons
JP6793112B2 (ja) * 2014-08-01 2020-12-02 アリオサ ダイアグノスティックス インコーポレイテッドAriosa Diagnostics,Inc. 胎児コピー数変異の統計的尤度を提供するアッセイ方法および胎児染色体異数性の尤度決定のためのアッセイ方法
ES2828959T3 (es) 2014-08-22 2021-05-28 Cepheid Métodos para detectar la influenza
WO2016069853A2 (en) 2014-10-30 2016-05-06 Cepheid Methods of detecting ebola
HK1247255A1 (zh) 2014-12-15 2018-09-21 Cepheid 底数大於2的指数核酸扩增
KR101742681B1 (ko) 2015-01-30 2017-06-01 에스디 바이오센서 주식회사 상보적 염기서열 내지는 미스-매치된 염기를 포함하는 상보적인 염기서열과 연결된 pcr 프라이머 및 이를 이용한 핵산 증폭 방법
CN107250445A (zh) 2015-02-27 2017-10-13 富鲁达公司 用于高通量研究的单个细胞核酸
EP3307885B1 (en) 2015-06-15 2020-10-21 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
WO2017123696A1 (en) 2016-01-12 2017-07-20 Interleukin Genetics, Inc. Methods for predicting response to treatment
WO2017189906A1 (en) 2016-04-27 2017-11-02 Mira Dx, Inc. Immune-based treatment of kras-variant cancer patients
JP2018068268A (ja) * 2016-11-04 2018-05-10 株式会社Gsp研究所 染色体の構造異常を迅速に検出するための方法、促進剤およびそれを含むキット
AU2017376118B2 (en) 2016-12-12 2022-09-01 Cepheid Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or mRNA expression levels in an automated reaction cartridge
JP2020503857A (ja) 2016-12-12 2020-02-06 セファイド 自動反応カートリッジにおける統合化イムノpcr及び核酸分析
US10337070B2 (en) 2017-01-12 2019-07-02 Cardioforecast Ltd. Methods and kits for treating cardiovascular disease
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
WO2019213619A1 (en) 2018-05-04 2019-11-07 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
CN112770776B (zh) 2018-07-30 2025-08-19 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
CA3118000A1 (en) 2018-10-29 2020-05-07 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers
WO2020163630A1 (en) 2019-02-06 2020-08-13 Singular Genomics Systems, Inc. Compositions and methods for nucleic acid sequencing
US20220249660A1 (en) 2019-06-06 2022-08-11 Sitokine Limited Compositions and methods for treating lung, colorectal and breast cancer
WO2021028469A1 (en) 2019-08-12 2021-02-18 Sitokine Limited Compositions and methods for treating cytokine release syndrome and neurotoxicity
US12577514B2 (en) 2019-10-29 2026-03-17 Toray Industries, Inc. Method of producing biochips
EP3901286A1 (en) 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
ES3030914T3 (en) 2019-11-04 2025-07-02 Mirnax Biosens S L Bivalent reverse primer
EP4097251A1 (en) 2020-01-29 2022-12-07 10X Genomics, Inc. Compositions and methods for analyte detection
WO2021205013A1 (en) 2020-04-09 2021-10-14 Sitokine Limited Compositions and methods for treating covid-19
CA3176983A1 (en) 2020-04-27 2021-11-04 Cepheid Exponential base-3 and greater nucleic acid amplification with cycling probe
BR112022026414A2 (pt) 2020-06-26 2023-03-14 Cepheid Métodos para detectar sars-cov-2, influenza e rsv
CN116250060A (zh) * 2020-07-27 2023-06-09 南京金斯瑞生物科技有限公司 一种半导体芯片的表面连接体及其制备方法和应用
BR112023006262A2 (pt) 2020-10-06 2023-05-09 Cepheid Métodos de diagnóstico de tuberculose e de diferenciação entre tuberculose ativa e latente
WO2022261366A1 (en) * 2021-06-09 2022-12-15 Avery Digital Data, Inc. Electronic devices for polymer synthesis and assembly
US20230140360A1 (en) 2021-10-22 2023-05-04 Cepheid Compositions and methods of diagnosing and treating tuberculosis
KR20250017227A (ko) 2022-05-19 2025-02-04 세페이드 Mvp 카트리지 및 사용 및 제조 방법
EP4587840A1 (en) 2022-09-15 2025-07-23 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
US20240218467A1 (en) 2022-12-16 2024-07-04 Cepheid Mpox clade ii biomarker panel
IL322301A (en) 2023-01-23 2025-09-01 Next Gen Diagnostics Llc Methods for rapid detection of antibiotic resistance in Escherichia coli
EP4658408A2 (en) 2023-01-30 2025-12-10 Cepheid Respiratory biomarker panel
WO2025006633A1 (en) 2023-06-27 2025-01-02 Cepheid Pancoronavirus biomarker panel
US20250171840A1 (en) 2023-09-18 2025-05-29 Cepheid Three-phase nested amplification and multi-phasic detection
CN117358168A (zh) * 2023-10-24 2024-01-09 苏州拉索生物芯片科技有限公司 一种改性二氧化硅微球的制备方法及应用
WO2025101548A2 (en) 2023-11-08 2025-05-15 Cepheid Her2 low quantification method
WO2025175133A1 (en) 2024-02-14 2025-08-21 Cepheid Melt shape genotyping
WO2025179142A1 (en) 2024-02-22 2025-08-28 Cepheid Maximizing optical channel capabilities for target detection
WO2025207561A1 (en) 2024-03-27 2025-10-02 Cepheid Isothermal amplification using novel synthetic oligonucleotides
WO2026019604A1 (en) 2024-07-19 2026-01-22 Cepheid Exponential base-x amplification with hairpin nested primers and universal flanking primers
WO2026055639A1 (en) 2024-09-06 2026-03-12 Cepheid Sample in sequencing-ready library out automated library preparation solution

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997031256A2 (en) * 1996-02-09 1997-08-28 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1998003673A1 (en) * 1996-07-19 1998-01-29 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction

Family Cites Families (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258506A (en) * 1984-10-16 1993-11-02 Chiron Corporation Photolabile reagents for incorporation into oligonucleotide chains
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5418149A (en) * 1990-07-24 1995-05-23 Hoffmann-La Roche Inc. Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) * 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5700637A (en) * 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
AU5640090A (en) * 1989-03-21 1990-11-05 Collaborative Research Inc. A dna diagnostic test using an exonuclease activity
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5527681A (en) * 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
CA2046713A1 (en) * 1990-10-16 1992-04-17 Richard M. Martinelli Amplification of midivariant dna templates
EP0834575B1 (en) 1990-12-06 2001-11-28 Affymetrix, Inc. (a Delaware Corporation) Identification of nucleic acids in samples
WO1992011390A1 (en) * 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
US5290925A (en) * 1990-12-20 1994-03-01 Abbott Laboratories Methods, kits, and reactive supports for 3' labeling of oligonucleotides
RU1794088C (ru) 1991-03-18 1993-02-07 Институт Молекулярной Биологии Ан@ Ссср Способ определени нуклеотидной последовательности ДНК и устройство дл его осуществлени
WO1992021079A1 (en) * 1991-05-24 1992-11-26 The President And Fellows Of Harvard College Parallel sequential reactor
US5278298A (en) * 1991-05-29 1994-01-11 Merck & Co., Inc. Eimeria brunetti 16s rDNA probes
DE69213112T2 (de) * 1991-06-20 1997-04-03 Hoffmann La Roche Verbesserte Methoden zur Nukleinsäure-Amplifizierung
US5371241A (en) * 1991-07-19 1994-12-06 Pharmacia P-L Biochemicals Inc. Fluorescein labelled phosphoramidites
AU2872092A (en) * 1991-10-15 1993-05-21 Multilyte Limited Binding assay employing labelled reagent
US5594121A (en) * 1991-11-07 1997-01-14 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
EP0675966B1 (en) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5470705A (en) * 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
EP0636186B1 (en) 1992-04-03 1998-11-25 The Perkin-Elmer Corporation Probe composition and method
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
US6180338B1 (en) * 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
WO1994009022A1 (en) 1992-10-09 1994-04-28 Oncor, Inc. Methods for the detection of chromosome structural abnormalities by in situ hybridization to fixed tissue
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
JP2575270B2 (ja) 1992-11-10 1997-01-22 浜松ホトニクス株式会社 核酸の塩基配列決定方法、単一分子検出方法、その装置及び試料の作成方法
EP0686200A4 (en) 1993-01-27 1997-07-02 Oncor Inc AMPLIFICATION OF NUCLEIC ACID SEQUENCES
WO1994017206A1 (en) 1993-01-27 1994-08-04 Oncor, Inc. Method for amplifying nucleic acid sequences
US5593840A (en) * 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
US5593826A (en) * 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5415839A (en) 1993-10-21 1995-05-16 Abbott Laboratories Apparatus and method for amplifying and detecting target nucleic acids
US6156501A (en) * 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5352582A (en) * 1993-10-28 1994-10-04 Hewlett-Packard Company Holographic based bio-assay
US5429807A (en) * 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5834181A (en) 1994-07-28 1998-11-10 Genzyme Corporation High throughput screening method for sequences or genetic alterations in nucleic acids
DE69528706T2 (de) * 1994-08-19 2003-06-12 Pe Corp. (Ny), Foster City Gekoppeltes ampflikation- und ligationverfahren
US5648213A (en) 1994-08-30 1997-07-15 Beckman Instruments, Inc. Compositions and methods for use in detection of analytes
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5512441A (en) * 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method
US5667974A (en) * 1995-06-07 1997-09-16 Abbott Laboratories Method for detecting nucleic acid sequences using competitive amplification
US5723320A (en) * 1995-08-29 1998-03-03 Dehlinger; Peter J. Position-addressable polynucleotide arrays
DK0862656T3 (da) * 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
EP2369007B1 (en) * 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6682918B1 (en) * 1996-07-19 2004-01-27 Arch Development Corporation Bacterial sucrose synthase compositions and methods of use
US5837659A (en) * 1997-08-21 1998-11-17 International Flavors & Fragrances Inc. Process for producing C9 C11 and C13 alkanols and microorganism capable of the same
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997031256A2 (en) * 1996-02-09 1997-08-28 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1998003673A1 (en) * 1996-07-19 1998-01-29 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction

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