JP4060892B2 - タンパク質のin vitroディスプレイ及び進化のための選択粒子としてのリボソーム複合体 - Google Patents

タンパク質のin vitroディスプレイ及び進化のための選択粒子としてのリボソーム複合体 Download PDF

Info

Publication number
JP4060892B2
JP4060892B2 JP50038699A JP50038699A JP4060892B2 JP 4060892 B2 JP4060892 B2 JP 4060892B2 JP 50038699 A JP50038699 A JP 50038699A JP 50038699 A JP50038699 A JP 50038699A JP 4060892 B2 JP4060892 B2 JP 4060892B2
Authority
JP
Japan
Prior art keywords
mrna
protein
dna
peptide
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP50038699A
Other languages
English (en)
Japanese (ja)
Other versions
JP2002500514A (ja
JP2002500514A5 (enExample
Inventor
マイケル ジョン タウシッグ
ミンユー ヘー
Original Assignee
ディサーナ リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27268863&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP4060892(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9710829.4A external-priority patent/GB9710829D0/en
Priority claimed from GBGB9724850.4A external-priority patent/GB9724850D0/en
Priority claimed from GBGB9804195.7A external-priority patent/GB9804195D0/en
Application filed by ディサーナ リミテッド filed Critical ディサーナ リミテッド
Publication of JP2002500514A publication Critical patent/JP2002500514A/ja
Publication of JP2002500514A5 publication Critical patent/JP2002500514A5/ja
Application granted granted Critical
Publication of JP4060892B2 publication Critical patent/JP4060892B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1041Ribosome/Polysome display, e.g. SPERT, ARM
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP50038699A 1997-05-28 1998-05-28 タンパク質のin vitroディスプレイ及び進化のための選択粒子としてのリボソーム複合体 Expired - Fee Related JP4060892B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GBGB9710829.4A GB9710829D0 (en) 1997-05-28 1997-05-28 Selection particles for in vitro display and evolution of antibody combining sites
GB9710829.4 1997-05-28
GB9724850.4 1997-11-26
GBGB9724850.4A GB9724850D0 (en) 1997-11-26 1997-11-26 Selection particles for in vitro display and evolution of antibody combining sites
GB9804195.7 1998-02-28
GBGB9804195.7A GB9804195D0 (en) 1998-02-28 1998-02-28 Selection particles for in vitro display and evolution of antibody combining sites
PCT/GB1998/001564 WO1998054312A1 (en) 1997-05-28 1998-05-28 Ribosome complexes as selection particles for in vitro display and evolution of proteins

Publications (3)

Publication Number Publication Date
JP2002500514A JP2002500514A (ja) 2002-01-08
JP2002500514A5 JP2002500514A5 (enExample) 2005-12-08
JP4060892B2 true JP4060892B2 (ja) 2008-03-12

Family

ID=27268863

Family Applications (1)

Application Number Title Priority Date Filing Date
JP50038699A Expired - Fee Related JP4060892B2 (ja) 1997-05-28 1998-05-28 タンパク質のin vitroディスプレイ及び進化のための選択粒子としてのリボソーム複合体

Country Status (10)

Country Link
US (1) US6620587B1 (enExample)
EP (1) EP0985032B2 (enExample)
JP (1) JP4060892B2 (enExample)
AT (1) ATE250668T1 (enExample)
CA (1) CA2291721C (enExample)
DE (1) DE69818458T3 (enExample)
DK (1) DK0985032T4 (enExample)
ES (1) ES2209145T5 (enExample)
PT (1) PT985032E (enExample)
WO (1) WO1998054312A1 (enExample)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4854999A (en) * 1998-07-02 2000-01-24 Invitro Diagnostics, Inc. Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule
DE19932688B4 (de) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen
GB9917027D0 (en) * 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
EP1276900A2 (en) 2000-01-11 2003-01-22 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
CA2402452A1 (en) 2000-03-31 2001-10-11 Cambridge Antibody Technology Limited Improvements to ribosome display
EP1652919A1 (en) * 2000-03-31 2006-05-03 Cambridge Antibody Technology LTD Improvements to ribosome display
US7129326B2 (en) 2000-04-14 2006-10-31 Genencor International, Inc. Methods for selective targeting
US20020098524A1 (en) 2000-04-14 2002-07-25 Murray Christopher J. Methods for selective targeting
EP1372721A4 (en) 2001-03-07 2008-04-02 Childrens Medical Center METHOD FOR SCREENING PEPTIDE DISPLAY LIBRARIES WITH A MINIMUM DISPLAY
EP1401850A1 (en) * 2001-06-20 2004-03-31 Nuevolution A/S Nucleoside derivatives for library preparation
US20060234231A1 (en) * 2001-06-20 2006-10-19 Nuevolution A/S Microarrays displaying encoded molecules
DE10145226A1 (de) * 2001-09-13 2003-04-10 Lifebits Ag Herstellung von trägergebundenen Molekülen
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
DE20200926U1 (de) * 2002-01-23 2002-04-18 Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz Festwalzgerät einer Festwalzmaschine für Kurbelwellen
EP1490384A2 (en) * 2002-03-15 2004-12-29 Nuevolution A/S A building block forming a c-c bond upon reaction
WO2003078626A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block capable of transferring a functional entity
CN101006177B (zh) 2002-03-15 2011-10-12 纽韦卢森公司 改善的用于合成模制分子的方法
WO2003078446A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond upon reaction
AU2003247266A1 (en) * 2002-08-01 2004-02-23 Nuevolution A/S Multi-step synthesis of templated molecules
US7842476B2 (en) * 2002-09-06 2010-11-30 Isogenica Limited In vitro peptide expression library
CN106337046B (zh) 2002-10-30 2021-03-23 纽韦卢森公司 合成双功能复合物的方法
WO2004048399A2 (en) 2002-11-25 2004-06-10 Genencor International, Inc. Skin or hair binding peptides
ATE450609T1 (de) * 2002-12-19 2009-12-15 Nuevolution As Durch quasizufallsstrukturen und funktionen geführte synthesemethode
WO2004074501A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S A method for obtaining structural information about an encoded molecule
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
DE602004021094D1 (de) * 2003-03-19 2009-06-25 Discerna Ltd Gewinnung von dna
US7915201B2 (en) * 2003-03-20 2011-03-29 Nuevolution A/S Ligational encoding of small molecules
DE10324447A1 (de) 2003-05-28 2004-12-30 Scil Proteins Gmbh Generierung künstlicher Bindungsproteine auf der Grundlage von Ubiquitin
GB0316314D0 (en) * 2003-07-12 2003-08-13 Univ Belfast Method
ATE447626T1 (de) 2003-09-18 2009-11-15 Nuevolution As Methode zur gewinnung struktureller informationen kodierter moleküle und zur selektion von verbindungen
US20090239211A1 (en) * 2004-02-17 2009-09-24 Nuevolution A/S Method For Enrichment Involving Elimination By Mismatch Hybridisation
DE602005017370D1 (de) * 2004-03-22 2009-12-10 Nuevolution As Ligationscodierung unter verwendung von oligonukleotidbausteinen
US20050250112A1 (en) * 2004-05-07 2005-11-10 Padmabandu Gothami A Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species
US9006393B1 (en) 2004-08-26 2015-04-14 Patricia L. Clark Molecular constructs and uses thereof in ribosomal translational events
DE102004049479A1 (de) 2004-10-11 2006-04-13 Scil Proteins Gmbh Proteinkonjugate zur Verwendung in Therapie, Diagnose und Chromatographie
LT3018206T (lt) 2005-12-01 2021-12-10 Nuevolution A/S Fermentiniai kodavimo būdai, skirti didelių bibliotekų efektyviai sintezei
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
US7777002B2 (en) * 2006-06-29 2010-08-17 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7910695B2 (en) 2006-06-29 2011-03-22 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7879975B2 (en) * 2006-06-29 2011-02-01 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7923533B2 (en) * 2006-06-29 2011-04-12 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7816101B2 (en) * 2006-06-29 2010-10-19 The Invention Science Fund I, Llc Apparatus for arbitrary peptide synthesis
EP1925664A1 (en) 2006-11-15 2008-05-28 Scil proteins GmbH Artificial binding proteins based on a modified alpha helical region of ubiquitin
US8865179B2 (en) * 2008-01-26 2014-10-21 Swey-Shen Alexchen Aptameric IgE peptides in a protein scaffold as an allergy vaccine
KR20110036638A (ko) * 2008-07-25 2011-04-07 리차드 더블유. 와그너 단백질 스크리닝 방법
CN102227638B (zh) * 2008-09-30 2015-05-20 Abbvie公司 Rna展示的改良方法
BR112012014194A2 (pt) 2009-12-14 2017-01-10 Scil Proteins Gmbh um método para identificar proteínas ubiquitinas modificadas hetero-multiméricas com capacidade de ligação a ligandos
EP2546342A4 (en) 2010-03-11 2013-12-04 Riken METHOD FOR SELECTION OF A POLYPEPTIDE SEQUENCE, METAL OXIDE OR SILICON WITH A BONDING SPEPTIDE AND USE THEREOF
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
CA2832672A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
GB201011971D0 (en) 2010-07-15 2010-09-01 Olink Ab Methods and product
US10669569B2 (en) 2010-10-15 2020-06-02 Navinci Diagnostics Ab Dynamic range methods
EP2466310A1 (en) 2010-12-17 2012-06-20 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH Means and methods for detecting double-strand breaks
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
JP5948673B2 (ja) 2011-03-10 2016-07-06 ジーンフロンティア株式会社 リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
WO2012161227A1 (ja) 2011-05-23 2012-11-29 独立行政法人理化学研究所 核酸構築物、核酸-蛋白質複合体、及びその利用
EP2721152B1 (en) 2011-06-15 2019-03-27 Navigo Proteins GmbH Dimeric binding proteins based on modified ubiquitins
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
US10421958B2 (en) 2014-02-05 2019-09-24 Molecular Templates, Inc. Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity
WO2016062989A1 (en) * 2014-10-22 2016-04-28 Crescendo Biologics Limited Human vh domain scaffolds
JP6738340B2 (ja) 2015-02-06 2020-08-12 ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH 新規なegfr結合タンパク質
ES2916101T3 (es) 2015-07-16 2022-06-28 Navigo Proteins Gmbh Nuevas proteínas de unión a inmunoglobulina y su uso en la purificación por afinidad
JP2018520675A (ja) 2015-07-20 2018-08-02 ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH ジユビキチン変異タンパク質をベースとした新規結合タンパク質及びその生成方法
MY193806A (en) 2015-09-24 2022-10-27 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
WO2018057051A1 (en) 2016-09-24 2018-03-29 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
JP2019526526A (ja) 2016-05-04 2019-09-19 ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH ペプチドリンカーを含む化学的部分の部位特異的カップリングのための標的化合物
DK3497117T5 (da) 2016-08-11 2024-09-02 Navigo Proteins Gmbh Nye alkalisk stabile immunglobulin-bindende proteiner
EP3706804B1 (en) 2017-11-07 2022-02-23 Navigo Proteins GmbH Fusion proteins with specificity for ed-b and long serum half-life for diagnosis or treatment of cancer
WO2021030271A2 (en) 2019-08-09 2021-02-18 Nutcracker Therapeutics, Inc. Methods and apparatuses for manufacturing for removing material from a therapeutic composition
EP4382534A1 (en) 2022-12-06 2024-06-12 Biomérieux Synthetic peptide for use in an immunomolecular assay
CN120858284A (zh) 2023-01-27 2025-10-28 生物梅里埃公司 用于更快生物标志物检测的改良的免疫分子测定
WO2025111396A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Molecular barcode set and use thereof in multiplex proximity detection assays

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06178688A (ja) 1992-12-11 1994-06-28 Mitsui Toatsu Chem Inc E型緑膿菌を抗原とするヒト抗体をコードする遺伝子
AU8124694A (en) * 1993-10-29 1995-05-22 Affymax Technologies N.V. In vitro peptide and antibody display libraries
CA2402452A1 (en) 2000-03-31 2001-10-11 Cambridge Antibody Technology Limited Improvements to ribosome display

Also Published As

Publication number Publication date
CA2291721A1 (en) 1998-12-03
AU7666698A (en) 1998-12-30
JP2002500514A (ja) 2002-01-08
PT985032E (pt) 2004-02-27
AU725957B2 (en) 2000-10-26
DK0985032T3 (da) 2004-02-02
CA2291721C (en) 2011-08-09
DE69818458T2 (de) 2004-07-08
DK0985032T4 (da) 2009-08-31
DE69818458D1 (de) 2003-10-30
EP0985032B1 (en) 2003-09-24
WO1998054312A1 (en) 1998-12-03
US6620587B1 (en) 2003-09-16
EP0985032A1 (en) 2000-03-15
ES2209145T5 (es) 2009-10-30
ES2209145T3 (es) 2004-06-16
EP0985032B2 (en) 2009-06-03
ATE250668T1 (de) 2003-10-15
DE69818458T3 (de) 2009-12-17

Similar Documents

Publication Publication Date Title
JP4060892B2 (ja) タンパク質のin vitroディスプレイ及び進化のための選択粒子としてのリボソーム複合体
US5922545A (en) In vitro peptide and antibody display libraries
US10329555B2 (en) High throughput generation and affinity maturation of humanized antibody
JP3507073B2 (ja) 特異的結合対の成員の製造方法
US20020068276A1 (en) Methods for selecting functional polypeptides
He et al. Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display
JP7772379B2 (ja) 抗原特異的結合ポリペプチド遺伝子ディスプレイベクターの構築方法および用途
US20020076692A1 (en) Ribosome display
Lamminmäki et al. Expanding the conformational diversity by random insertions to CDRH2 results in improved anti-estradiol antibodies
WO1995011922A1 (en) In vitro peptide and antibody display libraries
KR20110076906A (ko) 개선된 rna 디스플레이 방법
JP2003536048A (ja) タンパク質の単離および分析
EP2970952B1 (en) An integrated system for library construction, affinity binder screening and expression thereof
He et al. Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system
Contreras-Martínez et al. Intracellular ribosome display via SecM translation arrest as a selection for antibodies with enhanced cytosolic stability
EP3526330A1 (en) Triple vector for expressing antibody molecules in full therapeutic format
AU2003253193B2 (en) Novel tricistronic vectors and uses therefor
AU725957C (en) Ribosome complexes as selection particles for in vitro display and evolution of proteins
CN111201239A (zh) 用于开发特异于表位翻译后修饰状态的抗体的方法和组合物
US12503693B2 (en) Integrated system for library construction, affinity binder screening and expression thereof
WO2004087914A1 (ja) 抗体作製方法
Deen Isolation of Human Endoplasmic Reticulum Antibodies Using Phage Display Technology
WO2003097796A2 (en) T7 bacteriophage display of fabs

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20050527

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20050527

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20070710

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20071010

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20071127

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20071221

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101228

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101228

Year of fee payment: 3

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101228

Year of fee payment: 3

R371 Transfer withdrawn

Free format text: JAPANESE INTERMEDIATE CODE: R371

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101228

Year of fee payment: 3

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101228

Year of fee payment: 3

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101228

Year of fee payment: 3

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20111228

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121228

Year of fee payment: 5

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121228

Year of fee payment: 5

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20131228

Year of fee payment: 6

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees