DE69818458D1 - Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinen - Google Patents
Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinenInfo
- Publication number
- DE69818458D1 DE69818458D1 DE69818458T DE69818458T DE69818458D1 DE 69818458 D1 DE69818458 D1 DE 69818458D1 DE 69818458 T DE69818458 T DE 69818458T DE 69818458 T DE69818458 T DE 69818458T DE 69818458 D1 DE69818458 D1 DE 69818458D1
- Authority
- DE
- Germany
- Prior art keywords
- complexes
- protein
- peptide
- mrna
- vitro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9710829 | 1997-05-28 | ||
GBGB9710829.4A GB9710829D0 (en) | 1997-05-28 | 1997-05-28 | Selection particles for in vitro display and evolution of antibody combining sites |
GB9724850 | 1997-11-26 | ||
GBGB9724850.4A GB9724850D0 (en) | 1997-11-26 | 1997-11-26 | Selection particles for in vitro display and evolution of antibody combining sites |
GB9804195 | 1998-02-28 | ||
GBGB9804195.7A GB9804195D0 (en) | 1998-02-28 | 1998-02-28 | Selection particles for in vitro display and evolution of antibody combining sites |
PCT/GB1998/001564 WO1998054312A1 (en) | 1997-05-28 | 1998-05-28 | Ribosome complexes as selection particles for in vitro display and evolution of proteins |
Publications (3)
Publication Number | Publication Date |
---|---|
DE69818458D1 true DE69818458D1 (de) | 2003-10-30 |
DE69818458T2 DE69818458T2 (de) | 2004-07-08 |
DE69818458T3 DE69818458T3 (de) | 2009-12-17 |
Family
ID=27268863
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69818458T Expired - Lifetime DE69818458T3 (de) | 1997-05-28 | 1998-05-28 | Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinen |
Country Status (10)
Country | Link |
---|---|
US (1) | US6620587B1 (de) |
EP (1) | EP0985032B2 (de) |
JP (1) | JP4060892B2 (de) |
AT (1) | ATE250668T1 (de) |
CA (1) | CA2291721C (de) |
DE (1) | DE69818458T3 (de) |
DK (1) | DK0985032T4 (de) |
ES (1) | ES2209145T5 (de) |
PT (1) | PT985032E (de) |
WO (1) | WO1998054312A1 (de) |
Families Citing this family (71)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU4854999A (en) * | 1998-07-02 | 2000-01-24 | Invitro Diagnostics, Inc. | Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule |
DE19932688B4 (de) | 1999-07-13 | 2009-10-08 | Scil Proteins Gmbh | Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen |
GB9917027D0 (en) * | 1999-07-20 | 1999-09-22 | Affibody Technology Sweeden Ab | In vitro selection and optional identification of polypeptides using solid support carriers |
US20010039014A1 (en) * | 2000-01-11 | 2001-11-08 | Maxygen, Inc. | Integrated systems and methods for diversity generation and screening |
JP2003529369A (ja) * | 2000-03-31 | 2003-10-07 | ケンブリッジ アンティボディー テクノロジー リミティド | リボソーム・ディスプレイの改良 |
EP1652919A1 (de) * | 2000-03-31 | 2006-05-03 | Cambridge Antibody Technology LTD | Verbesserungen in Bezug auf Ribosom-Komplexe als Selektionspartikel |
PT1360500E (pt) | 2000-04-14 | 2010-04-19 | Genencor Int | Métodos para direccionamento selectivo |
US7129326B2 (en) | 2000-04-14 | 2006-10-31 | Genencor International, Inc. | Methods for selective targeting |
BR0208051A (pt) | 2001-03-07 | 2006-02-21 | Childrens Medical Center | método de seleção de bibliotecas de apresentação de peptìdeo que usa apresentação em minicélula |
US20060234231A1 (en) * | 2001-06-20 | 2006-10-19 | Nuevolution A/S | Microarrays displaying encoded molecules |
WO2002102820A1 (en) | 2001-06-20 | 2002-12-27 | Nuevolution A/S | Nucleoside derivatives for library preparation |
DE10145226A1 (de) * | 2001-09-13 | 2003-04-10 | Lifebits Ag | Herstellung von trägergebundenen Molekülen |
DE10147074A1 (de) * | 2001-09-25 | 2003-05-08 | Beru Ag | Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung |
DE20200926U1 (de) * | 2002-01-23 | 2002-04-18 | Hegenscheidt Mfd Gmbh & Co Kg | Festwalzgerät einer Festwalzmaschine für Kurbelwellen |
WO2003078446A2 (en) * | 2002-03-15 | 2003-09-25 | Nuevolution A/S | A building block forming a c-c or a c-hetero atom bond upon reaction |
ATE414769T1 (de) | 2002-03-15 | 2008-12-15 | Nuevolution As | Eine verbesserte methode zur synthese von matritzenabhängigen molekülen |
AU2003253069A1 (en) * | 2002-03-15 | 2003-09-29 | Nuevolution A/S | A building block forming a c-c bond upon reaction |
AU2003218630A1 (en) * | 2002-03-15 | 2003-09-29 | Nuevolution A/S | A building block capable of functional entity transfer to nucleophil |
WO2004013070A2 (en) * | 2002-08-01 | 2004-02-12 | Nuevolution A/S | Multi-step synthesis of templated molecules |
ATE488586T1 (de) * | 2002-09-06 | 2010-12-15 | Isogenica Ltd | In vitro peptid-expressionsbank |
LT2348125T (lt) | 2002-10-30 | 2017-09-11 | Nuevolution A/S | Bifunkcinio komplekso sintezės būdas |
US20060171885A1 (en) | 2002-11-25 | 2006-08-03 | Giselle Janssen | Skin or hair binding peptides |
ATE450609T1 (de) * | 2002-12-19 | 2009-12-15 | Nuevolution As | Durch quasizufallsstrukturen und funktionen geführte synthesemethode |
WO2004074429A2 (en) * | 2003-02-21 | 2004-09-02 | Nuevolution A/S | Method for producing second-generation library |
US20060269920A1 (en) * | 2003-02-21 | 2006-11-30 | Nuevolution A/S | Method for obtaining structural information about an encoded molecule |
EP1604044B1 (de) * | 2003-03-19 | 2009-05-13 | Discerna Limited | Gewinnung von dna |
EP1608748B1 (de) * | 2003-03-20 | 2009-03-04 | Nuevolution A/S | Ligationsvermittelnde codierung von kleinen molekülen |
DE10324447A1 (de) | 2003-05-28 | 2004-12-30 | Scil Proteins Gmbh | Generierung künstlicher Bindungsproteine auf der Grundlage von Ubiquitin |
GB0316314D0 (en) * | 2003-07-12 | 2003-08-13 | Univ Belfast | Method |
US11118215B2 (en) * | 2003-09-18 | 2021-09-14 | Nuevolution A/S | Method for obtaining structural information concerning an encoded molecule and method for selecting compounds |
EP1723255B1 (de) * | 2004-02-17 | 2010-12-29 | Nuevolution A/S | Anreicherungsverfahren mit eliminierung durch fehlpaarungshybridisierung |
DE602005017370D1 (de) * | 2004-03-22 | 2009-12-10 | Nuevolution As | Ligationscodierung unter verwendung von oligonukleotidbausteinen |
US20050250112A1 (en) * | 2004-05-07 | 2005-11-10 | Padmabandu Gothami A | Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species |
US9006393B1 (en) | 2004-08-26 | 2015-04-14 | Patricia L. Clark | Molecular constructs and uses thereof in ribosomal translational events |
DE102004049479A1 (de) | 2004-10-11 | 2006-04-13 | Scil Proteins Gmbh | Proteinkonjugate zur Verwendung in Therapie, Diagnose und Chromatographie |
DK2341140T3 (da) | 2005-12-01 | 2017-11-06 | Nuevolution As | Fremgangsmåde til enzymatisk kodning ved effektiv syntese af store biblioteker |
GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
US7923533B2 (en) * | 2006-06-29 | 2011-04-12 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
US7888465B2 (en) * | 2006-06-29 | 2011-02-15 | The Invention Science Fund, I, LLC | Methods for arbitrary peptide synthesis |
US7910695B2 (en) | 2006-06-29 | 2011-03-22 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
US7777002B2 (en) * | 2006-06-29 | 2010-08-17 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
US7879975B2 (en) * | 2006-06-29 | 2011-02-01 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
EP1925664A1 (de) | 2006-11-15 | 2008-05-28 | Scil proteins GmbH | Künstliche Bindungsproteine auf Grundlage einer modifizierten alpha-Helix Region von Ubiquitin |
US8865179B2 (en) * | 2008-01-26 | 2014-10-21 | Swey-Shen Alexchen | Aptameric IgE peptides in a protein scaffold as an allergy vaccine |
EP3629022A1 (de) | 2008-07-25 | 2020-04-01 | Richard W. Wagner | Proteinscreeningverfahren |
KR20110076906A (ko) * | 2008-09-30 | 2011-07-06 | 아보트 러보러터리즈 | 개선된 rna 디스플레이 방법 |
US8748351B2 (en) | 2009-12-14 | 2014-06-10 | Scil Proteins Gmbh | Method for identifying hetero-multimeric modified ubiquitin proteins with binding capability to ligands |
JP5863045B2 (ja) | 2010-03-11 | 2016-02-16 | 国立研究開発法人理化学研究所 | ポリペプチド配列の選択方法、並びに金属酸化物または含珪素化合物結合ペプチドおよびその利用 |
GB201004292D0 (en) | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
SI2558577T1 (sl) | 2010-04-16 | 2019-05-31 | Nuevolution A/S | Bifunkcionalni kompleksi in metode za pripravo in uporabo takšnih kompleksov |
GB201011971D0 (en) | 2010-07-15 | 2010-09-01 | Olink Ab | Methods and product |
US10669569B2 (en) | 2010-10-15 | 2020-06-02 | Navinci Diagnostics Ab | Dynamic range methods |
EP2466310A1 (de) | 2010-12-17 | 2012-06-20 | Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH | Mittel und Verfahren für den Nachweis von mehrstrangigen Brüchen |
DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
GB201101621D0 (en) | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
JP5948673B2 (ja) | 2011-03-10 | 2016-07-06 | ジーンフロンティア株式会社 | リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法 |
GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
US9863936B2 (en) | 2011-05-23 | 2018-01-09 | Riken | Nucleic acid construct, nucleic acid-protein complex, and use thereof |
JP6146821B2 (ja) | 2011-06-15 | 2017-06-14 | ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH | 修飾ユビキチンに基づく二量体型結合タンパク質 |
GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
JP6814634B2 (ja) | 2014-02-05 | 2021-01-20 | モレキュラー テンプレーツ,インク.Molecular Templates, Inc. | リボ毒性の一時的な減少に基づき、細胞毒性組換えポリペプチドをスクリーニングし、選択し、同定する方法 |
EP3209776A1 (de) * | 2014-10-22 | 2017-08-30 | Crescendo Biologics Limited | Humane vh-domänengerüste |
CA2975362A1 (en) | 2015-02-06 | 2016-08-11 | Navigo Proteins Gmbh | Egfr binding proteins comprising ubiquitin muteins |
BR112018000477A2 (pt) | 2015-07-16 | 2018-09-18 | Navigo Proteins Gmbh | proteína de ligação de imunoglobulina não natural, composição, uso da proteína de ligação de ig não natural, método de purificação de afinidade de imunoglobulinas, método de geração de uma proteína de ligação de imunoglobulina não natural, molécula de ácido nucleico, vetor, célula hospedeira ou um hospedeiro não humano, e método para a produção de uma proteína de ligação de imunoglobulina não natural |
JP2018520675A (ja) | 2015-07-20 | 2018-08-02 | ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH | ジユビキチン変異タンパク質をベースとした新規結合タンパク質及びその生成方法 |
ES2928681T3 (es) | 2015-09-24 | 2022-11-21 | Abvitro Llc | Conjugados de afinidad-oligonucleótido y usos de los mismos |
AU2017260274A1 (en) | 2016-05-04 | 2018-11-01 | Navigo Proteins Gmbh | Targeted compounds for the site-specific coupling of chemical moieties comprising a peptide linker |
CN109963864B (zh) | 2016-08-11 | 2024-01-02 | 瑞普利金公司 | 用于亲和色谱的碱性稳定性fc结合蛋白 |
BR112019005748A2 (pt) | 2016-09-24 | 2019-06-18 | Abvitro Llc | afinidade-conjugados de oligonucleotídeo e usos destes |
US11414466B2 (en) | 2017-11-07 | 2022-08-16 | Navigo Proteins Gmbh | Fusion proteins with specificity for ED-B and long serum half-life for diagnosis or treatment of cancer |
BR112022002365A2 (pt) | 2019-08-09 | 2022-04-26 | Nutcracker Therapeutics Inc | Métodos e aparelhos para fabricar e para remover material de uma composição terapêutica |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06178688A (ja) | 1992-12-11 | 1994-06-28 | Mitsui Toatsu Chem Inc | E型緑膿菌を抗原とするヒト抗体をコードする遺伝子 |
AU8124694A (en) * | 1993-10-29 | 1995-05-22 | Affymax Technologies N.V. | In vitro peptide and antibody display libraries |
JP2003529369A (ja) | 2000-03-31 | 2003-10-07 | ケンブリッジ アンティボディー テクノロジー リミティド | リボソーム・ディスプレイの改良 |
-
1998
- 1998-05-28 DK DK98924468T patent/DK0985032T4/da active
- 1998-05-28 EP EP98924468A patent/EP0985032B2/de not_active Expired - Lifetime
- 1998-05-28 AT AT98924468T patent/ATE250668T1/de active
- 1998-05-28 CA CA2291721A patent/CA2291721C/en not_active Expired - Fee Related
- 1998-05-28 WO PCT/GB1998/001564 patent/WO1998054312A1/en active IP Right Grant
- 1998-05-28 ES ES98924468T patent/ES2209145T5/es not_active Expired - Lifetime
- 1998-05-28 US US09/424,712 patent/US6620587B1/en not_active Expired - Lifetime
- 1998-05-28 PT PT98924468T patent/PT985032E/pt unknown
- 1998-05-28 DE DE69818458T patent/DE69818458T3/de not_active Expired - Lifetime
- 1998-05-28 JP JP50038699A patent/JP4060892B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
DE69818458T2 (de) | 2004-07-08 |
PT985032E (pt) | 2004-02-27 |
US6620587B1 (en) | 2003-09-16 |
ES2209145T5 (es) | 2009-10-30 |
ATE250668T1 (de) | 2003-10-15 |
EP0985032A1 (de) | 2000-03-15 |
AU725957B2 (en) | 2000-10-26 |
AU7666698A (en) | 1998-12-30 |
JP2002500514A (ja) | 2002-01-08 |
DE69818458T3 (de) | 2009-12-17 |
WO1998054312A1 (en) | 1998-12-03 |
EP0985032B1 (de) | 2003-09-24 |
EP0985032B2 (de) | 2009-06-03 |
DK0985032T3 (da) | 2004-02-02 |
JP4060892B2 (ja) | 2008-03-12 |
CA2291721C (en) | 2011-08-09 |
DK0985032T4 (da) | 2009-08-31 |
ES2209145T3 (es) | 2004-06-16 |
CA2291721A1 (en) | 1998-12-03 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8363 | Opposition against the patent | ||
8366 | Restricted maintained after opposition proceedings | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: CRESCENDO BIOLOGICS LTD., BABRAHAM, CAMBRIDGE, GB |
|
8328 | Change in the person/name/address of the agent |
Representative=s name: MARKS & CLERK (LUXEMBOURG) LLP, LUXEMBOURG, LU |