GB2338237A - In vitro peptide or protein expression library - Google Patents

In vitro peptide or protein expression library

Info

Publication number
GB2338237A
GB2338237A GB9911675A GB9911675A GB2338237A GB 2338237 A GB2338237 A GB 2338237A GB 9911675 A GB9911675 A GB 9911675A GB 9911675 A GB9911675 A GB 9911675A GB 2338237 A GB2338237 A GB 2338237A
Authority
GB
Grant status
Application
Patent type
Prior art keywords
expression library
protein expression
library
proteins
vitro peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9911675A
Other versions
GB9911675D0 (en )
GB2338237B (en )
Inventor
David Andrews
Rd-Ljungquist Elizabeth Hagg
Morten Isaksen
Bjorn H Lindqvist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Actinova Ltd
Original Assignee
Actinova Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00Bacteriophages
    • C12N2795/00011Bacteriophages
    • C12N2795/10011Bacteriophages dsDNA Bacteriophages
    • C12N2795/10111Myoviridae
    • C12N2795/10122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

The present invention provides a method of producing an in vitro peptide or protein expression library which displays a diverse population of peptides or proteins, wherein the peptides or proteins are specifically associated with the DNA encoding them through covalent protein:DNA binding expecially through the use of cis-acting proteins, the library itself, DNA molecules encoding the library and uses of the expression library.
GB9911675A 1997-02-18 1998-02-18 In vitro peptide or protein expression library Expired - Fee Related GB2338237B (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
GB9703369A GB9703369D0 (en) 1997-02-18 1997-02-18 Process
PCT/GB1998/000518 WO1998037186A1 (en) 1997-02-18 1998-02-18 In vitro peptide or protein expression library
GB9911675A GB2338237B (en) 1997-02-18 1998-02-18 In vitro peptide or protein expression library

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9911675A GB2338237B (en) 1997-02-18 1998-02-18 In vitro peptide or protein expression library

Publications (3)

Publication Number Publication Date
GB9911675D0 GB9911675D0 (en) 1999-07-21
GB2338237A true true GB2338237A (en) 1999-12-15
GB2338237B GB2338237B (en) 2001-02-28

Family

ID=26311017

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9911675A Expired - Fee Related GB2338237B (en) 1997-02-18 1998-02-18 In vitro peptide or protein expression library

Country Status (1)

Country Link
GB (1) GB2338237B (en)

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US7919313B2 (en) 2007-07-12 2011-04-05 Sangamo Biosciences, Inc. Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression
WO2011100058A1 (en) 2010-02-09 2011-08-18 Sangamo Biosciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
WO2011139349A1 (en) 2010-05-03 2011-11-10 Sangamo Biosciences, Inc. Compositions for linking zinc finger modules
EP2395081A1 (en) 2006-08-11 2011-12-14 Dow AgroSciences LLC Zinc finger nuclease-mediated homologous recombination
WO2012012667A2 (en) 2010-07-21 2012-01-26 Sangamo Biosciences, Inc. Methods and compositions for modification of a hla locus
WO2012012738A1 (en) 2010-07-23 2012-01-26 Sigma-Aldrich Co., Llc Genome editing using targeting endonucleases and single-stranded nucleic acids
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WO2013003767A2 (en) 2011-06-30 2013-01-03 Sigma-Aldrich Co. Llc Cells deficient in cmp-n-acetylneuraminic acid hydroxylase and/or glycoprotein alpha-1,3-galactosyltransferase
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US8399218B2 (en) 2007-09-27 2013-03-19 Dow Agrosciences, Llc Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes
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WO2014011901A2 (en) 2012-07-11 2014-01-16 Sangamo Biosciences, Inc. Methods and compositions for delivery of biologics
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US8772009B2 (en) 2009-05-18 2014-07-08 Sangamo Biosciences, Inc. Methods and compositions for increasing nuclease activity
US8771985B2 (en) 2010-04-26 2014-07-08 Sangamo Biosciences, Inc. Genome editing of a Rosa locus using zinc-finger nucleases
WO2014125668A1 (en) 2013-02-14 2014-08-21 国立大学法人大阪大学 Method for isolating specific genomic region using molecule binding specifically to endogenous dna sequence
US8841260B2 (en) 2012-02-29 2014-09-23 Sangamo Biosciences, Inc. Methods and compositions for treating Huntington's Disease
US8895264B2 (en) 2011-10-27 2014-11-25 Sangamo Biosciences, Inc. Methods and compositions for modification of the HPRT locus
US8956828B2 (en) 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
WO2015057976A1 (en) 2013-10-17 2015-04-23 Sangamo Biosciences, Inc. Delivery methods and compositions for nuclease-mediated genome engineering in hematopoietic stem cells
US9045763B2 (en) 2005-07-26 2015-06-02 Sangamo Biosciences, Inc. Linear donor constructs for targeted integration
WO2015089046A1 (en) 2013-12-09 2015-06-18 Sangamo Biosciences, Inc. Methods and compositions for treating hemophilia
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US9249428B2 (en) 2003-08-08 2016-02-02 Sangamo Biosciences, Inc. Methods and compositions for targeted genomic deletion
US9255250B2 (en) 2012-12-05 2016-02-09 Sangamo Bioscience, Inc. Isolated mouse or human cell having an exogenous transgene in an endogenous albumin gene
US9267123B2 (en) 2011-01-05 2016-02-23 Sangamo Biosciences, Inc. Methods and compositions for gene correction
US9376484B2 (en) 2012-01-11 2016-06-28 Sigma-Aldrich Co. Llc Production of recombinant proteins with simple glycoforms
US9394531B2 (en) 2008-05-28 2016-07-19 Sangamo Biosciences, Inc. Compositions for linking DNA-binding domains and cleavage domains
US9458205B2 (en) 2011-11-16 2016-10-04 Sangamo Biosciences, Inc. Modified DNA-binding proteins and uses thereof
WO2016161446A1 (en) 2015-04-03 2016-10-06 Dana-Farber Cancer Institute, Inc. Composition and methods of genome editing of b-cells
US9506120B2 (en) 2007-09-27 2016-11-29 Sangamo Biosciences, Inc. Rapid in vivo identification of biologically active nucleases
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US9757420B2 (en) 2014-07-25 2017-09-12 Sangamo Therapeutics, Inc. Gene editing for HIV gene therapy
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WO2018013840A1 (en) 2016-07-13 2018-01-18 Vertex Pharmaceuticals Incorporated Methods, compositions and kits for increasing genome editing efficiency
US9873894B2 (en) 2013-05-15 2018-01-23 Sangamo Therapeutics, Inc. Methods and compositions for treatment of a genetic condition
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US9914930B2 (en) 2012-09-07 2018-03-13 Dow Agrosciences Llc FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
US9937207B2 (en) 2013-03-21 2018-04-10 Sangamo Therapeutics, Inc. Targeted disruption of T cell receptor genes using talens
US9957501B2 (en) 2015-06-18 2018-05-01 Sangamo Therapeutics, Inc. Nuclease-mediated regulation of gene expression
US9957526B2 (en) 2013-10-17 2018-05-01 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
US9970001B2 (en) 2014-06-05 2018-05-15 Sangamo Therapeutics, Inc. Methods and compositions for nuclease design
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US10093940B2 (en) 2013-11-04 2018-10-09 Dow Agrosciences Llc Optimal maize loci
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US9249428B2 (en) 2003-08-08 2016-02-02 Sangamo Biosciences, Inc. Methods and compositions for targeted genomic deletion
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US9376685B2 (en) 2005-07-26 2016-06-28 Sangamo Biosciences, Inc. Linear donor constructs for targeted integration
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