JP2758495B2 - Hair restoration cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a hair-growth cosmetic having excellent hair-growth and hair-growth effects. More specifically, the present invention relates to a hair restoration cosmetic having an excellent hair-growth effect and an anti-hair loss effect.

[Problems to be solved by conventional technology and invention]

2. Description of the Related Art Conventionally, hair restoration cosmetics containing a scalp blood circulation promoting substance such as pepper tincture, assembly extract, Korean ginseng, nicotinic acid, and methyl nicotinate are well known.

However, these blood circulation promoting substances have strong skin irritation,
The compounding amount is restricted, the blood circulation promoting action is reduced in a short time, or the blood circulation promoting action is weak, and the substance is not effective enough for hair growth and hair loss preventing effects to be sufficiently exhibited. Was.

Therefore, the present inventors have conducted intensive studies on natural extracts, cultured cell extract substances, and cell activators which are highly effective when incorporated into hair nourishing cosmetics. It has been found that a hair nourishing cosmetic characterized by the following is extremely weak in skin irritation and has an effect of preventing hair growth and hair loss, thereby completing the present invention.

[Configuration of the invention]

The present invention is a hair restoration cosmetic comprising a hair papilla or a hair papilla cell.

The papilla or papilla cells used in the present invention are described, for example, in the Japanese Dermatological Association, Vol. 99, pp. 529-536, and can be obtained, for example, as follows.

(Preparation method of hair nipple)

The anagen hair is separated from the skin tissue piece and traversed immediately above the hair bulb. Separate from the hair bulb to the dermal papilla using two 1 ml syringes with a 20G needle.

Twenty obtained hair papilla were suspended in 1 ml of phosphate buffered saline and homogenized with a Polytron homogenizer.

(Preparation method of hair papilla cells)

Three to five hair papilla separated from the above hair bulb were arranged in a 35 mmφ petri dish for culture, and supplemented with 20% fetal calf serum, in a Dulbecco's modified Eagle's medium (DEMM) at 5% CO ₂ at 37 ° C. And cultured. After culturing for 5 to 7 days, the medium was changed every three days thereafter. At the time when sufficient cell growth was obtained (after 4-6 weeks), the cells were detached with an EDTA-trypsin solution and 2 ×
The cells were suspended in 10 ⁵ / ml DMEM and homogenized using a Teflon homogenizer.

The present inventors have conducted intensive studies on the above-mentioned constituents to be incorporated into the hair nourishing cosmetics, and have found that the excellent hair-growth effect of the present invention (mouse hair growth promoting effect, human hair growth promoting effect),
It was remarkably recognized to have a hair-growing effect.

That is, the components of the papilla act on the scalp to form hair follicles,
The excellent effect of normalizing the hair matrix metabolic function and improving the hair-growing effect and the hair loss-preventing effect could be obtained.

Further, the mixing ratio of the hair nipple of the hair restoration cosmetic is appropriately adjusted depending on the dosage form of the hair restoration cosmetic, etc., but it is sufficient that the content is 0.01 to 10.0 wt% based on the total amount. If the amount is less than the lower limit of the compounding ingredient, the effect intended by the present invention is not sufficient. On the other hand, if the amount exceeds the upper limit, the improvement of the effect corresponding to the increase cannot be expected.

The hair restoration cosmetic of the present invention can be made into a dosage form such as a hair tonic, a hair lotion, a hair cream or the like according to a conventional method. The hair restoration cosmetic of the present invention includes a pigment, a fragrance, a bactericide, a preservative, a keratolytic agent, an antiandrogen,
A hair restorer, an antioxidant, and the like can be appropriately compounded within a range that achieves the object of the present invention.

(Examples) Hereinafter, the present invention will be described in detail based on examples and comparative examples.

The mouse hair growth promotion test, human hair growth promotion test and practical test are shown below.

(1) Mouse hair growth promotion test The back skin from the tail of a ddy white mouse (male, 6 weeks old, average weight 30g) was cut with an electric hair clipper, and the hair was completely removed with a depilatory cream. Each sample of Comparative Example and 0.1 ml of each sample was applied to the skin of the test portion twice daily in the morning and evening. For each sample, 10 animals were used per group.

The evaluation of the hair growth effect was performed by comparing the hair length of the sample application part with the control group.

On the 15th day after the start of the experiment, the animals were sacrificed, and the skin at the test site was punched out and dried. The length of the 20 hairs was measured, and the average value was shown.

(2) Human hair growth promotion test Above the ears of the heads of 10 subjects who are male pattern baldness patients
To the test site where the hair at 5 cm position was shaved at two places on the left and right into a circular shape with a diameter of 1 cm, apply the sample of the example or the comparative example to the left side about 3 ml twice a day every morning and evening and compare with the untreated right side did. The effect was determined on the 28th day after the start of the test by shaving 20 hairs on each of the left and right test sites, and averaging the length of the 20 hairs on the left side (to which the example or comparative example was applied) (B ) Was divided by the average value (A) of the lengths of the 20 right hairs (untreated) to evaluate.

The judgment results were shown as the average of (B) / (A) of 10 test subjects.

(3) Human Practical Testing Method The effect of applying to the heads of 20 test subjects who are male pattern baldness patients twice daily in the morning and evening for 6 consecutive months was evaluated. The test results are for hair growth and hair loss prevention.
The number of respondents who answered that "hair was bristle or hair was increased" and "hair loss was reduced" were shown.

Examples 1-4, Comparative Example 1 [Oily Hair Tonic] In the following raw material composition, as shown in Table 1, hair papilla or hair papilla cell homogenate was blended to prepare each hair tonic. Was tested.

(1) Composition (2) Preparation method After uniformly dissolving the components (B) and (C), (A)
The components are mixed, stirred and dispersed, and then filled into a container.

(3) Characteristics The results of conducting various tests on each oily hair art tonic are shown in the right column of Table 1.

The hair growth cosmetics of the present invention of Examples 1 to 4 showed clearly good results.

Examples 5 to 6, Comparative Example 2 [Hair Cream] Each of the hair creams was prepared in the same manner as in Example 1, and various tests were carried out. The results are shown in the right column of Table 1.

(2) Preparation method The components (A) and (C), each of which was heated and dissolved at 80 ° C., were mixed. Then, while cooling with stirring, when the temperature reached 50 ° C, the component (C) was added, and stirring was further continued to a temperature of 30 ° C to prepare each hair cream.

(3) Properties As shown in Table 1, Examples 5 and 6, which are the hair growth cosmetics of the present invention, exhibited excellent effects.

[Effects of the Invention] As described above, it is apparent that the present invention provides a hair restoration cosmetic composition having excellent hair restoration and hair loss prevention effects.

Claims (1)

(57) [Claims] 1. A hair restoration cosmetic comprising a hair papilla or a hair papilla cell.