JP2021505141A5 - - Google Patents
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- Publication number
- JP2021505141A5 JP2021505141A5 JP2020530565A JP2020530565A JP2021505141A5 JP 2021505141 A5 JP2021505141 A5 JP 2021505141A5 JP 2020530565 A JP2020530565 A JP 2020530565A JP 2020530565 A JP2020530565 A JP 2020530565A JP 2021505141 A5 JP2021505141 A5 JP 2021505141A5
- Authority
- JP
- Japan
- Prior art keywords
- human
- item
- rodent
- gene
- light chain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims description 545
- 101710189008 Immunoglobulin kappa light chain Proteins 0.000 claims description 178
- 102100029567 Immunoglobulin kappa light chain Human genes 0.000 claims description 175
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 claims description 107
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 claims description 107
- 108060003951 Immunoglobulin Proteins 0.000 claims description 71
- 102000018358 immunoglobulin Human genes 0.000 claims description 71
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 54
- 108091026890 Coding region Proteins 0.000 claims description 45
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 45
- 210000004602 germ cell Anatomy 0.000 claims description 31
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 29
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 claims description 29
- 150000007523 nucleic acids Chemical group 0.000 claims description 12
- 230000000392 somatic effect Effects 0.000 claims description 11
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 10
- 241000699670 Mus sp. Species 0.000 claims description 8
- 238000012239 gene modification Methods 0.000 claims description 5
- 230000005017 genetic modification Effects 0.000 claims description 4
- 235000013617 genetically modified food Nutrition 0.000 claims description 4
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 claims 1
- 241000283984 Rodentia Species 0.000 description 477
- 210000004027 cell Anatomy 0.000 description 137
- 238000000034 method Methods 0.000 description 128
- 239000012634 fragment Substances 0.000 description 41
- 229920001184 polypeptide Polymers 0.000 description 37
- 108090000765 processed proteins & peptides Proteins 0.000 description 37
- 102000004196 processed proteins & peptides Human genes 0.000 description 37
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 description 36
- 241000282412 Homo Species 0.000 description 25
- 239000000427 antigen Substances 0.000 description 23
- 108091007433 antigens Proteins 0.000 description 23
- 102000036639 antigens Human genes 0.000 description 23
- 239000002773 nucleotide Substances 0.000 description 22
- 125000003729 nucleotide group Chemical group 0.000 description 22
- 108091008109 Pseudogenes Proteins 0.000 description 10
- 102000057361 Pseudogenes Human genes 0.000 description 10
- 102000001183 RAG-1 Human genes 0.000 description 10
- 108060006897 RAG1 Proteins 0.000 description 10
- 101001061851 Homo sapiens V(D)J recombination-activating protein 2 Proteins 0.000 description 9
- 102100029591 V(D)J recombination-activating protein 2 Human genes 0.000 description 9
- 230000003053 immunization Effects 0.000 description 9
- 108010029485 Protein Isoforms Proteins 0.000 description 5
- 102000001708 Protein Isoforms Human genes 0.000 description 5
- 210000000349 chromosome Anatomy 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 description 4
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000028993 immune response Effects 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- 101150075516 RAG2 gene Proteins 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024012752A JP7765514B2 (ja) | 2017-12-05 | 2024-01-31 | 操作された免疫グロブリンラムダ軽鎖を有する非ヒト動物及びその使用 |
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762594946P | 2017-12-05 | 2017-12-05 | |
| US201762594944P | 2017-12-05 | 2017-12-05 | |
| US62/594,946 | 2017-12-05 | ||
| US62/594,944 | 2017-12-05 | ||
| US201762609251P | 2017-12-21 | 2017-12-21 | |
| US201762609241P | 2017-12-21 | 2017-12-21 | |
| US62/609,241 | 2017-12-21 | ||
| US62/609,251 | 2017-12-21 | ||
| PCT/US2018/063841 WO2019113065A1 (en) | 2017-12-05 | 2018-12-04 | Non-human animals having an engineered immunoglobulin lambda light chain and uses thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024012752A Division JP7765514B2 (ja) | 2017-12-05 | 2024-01-31 | 操作された免疫グロブリンラムダ軽鎖を有する非ヒト動物及びその使用 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021505141A JP2021505141A (ja) | 2021-02-18 |
| JP2021505141A5 true JP2021505141A5 (enExample) | 2022-01-11 |
| JP7430636B2 JP7430636B2 (ja) | 2024-02-13 |
Family
ID=64734263
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020530565A Active JP7430636B2 (ja) | 2017-12-05 | 2018-12-04 | 操作された免疫グロブリンラムダ軽鎖を有する非ヒト動物及びその使用 |
| JP2024012752A Active JP7765514B2 (ja) | 2017-12-05 | 2024-01-31 | 操作された免疫グロブリンラムダ軽鎖を有する非ヒト動物及びその使用 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024012752A Active JP7765514B2 (ja) | 2017-12-05 | 2024-01-31 | 操作された免疫グロブリンラムダ軽鎖を有する非ヒト動物及びその使用 |
Country Status (24)
| Country | Link |
|---|---|
| US (3) | US11051498B2 (enExample) |
| EP (3) | EP4140297B1 (enExample) |
| JP (2) | JP7430636B2 (enExample) |
| KR (2) | KR20250022220A (enExample) |
| CN (2) | CN116058333A (enExample) |
| AU (2) | AU2018381316B2 (enExample) |
| BR (1) | BR112020011184A2 (enExample) |
| CA (1) | CA3084049A1 (enExample) |
| DK (2) | DK4140297T3 (enExample) |
| ES (2) | ES2928995T3 (enExample) |
| FI (1) | FI4140297T3 (enExample) |
| HR (2) | HRP20221459T1 (enExample) |
| HU (2) | HUE060608T2 (enExample) |
| IL (2) | IL318197A (enExample) |
| LT (2) | LT4140297T (enExample) |
| MX (2) | MX2020005830A (enExample) |
| PL (2) | PL3720279T3 (enExample) |
| PT (2) | PT4140297T (enExample) |
| RS (2) | RS67058B1 (enExample) |
| SG (1) | SG11202003044SA (enExample) |
| SI (2) | SI4140297T1 (enExample) |
| SM (2) | SMT202200448T1 (enExample) |
| TW (2) | TWI827567B (enExample) |
| WO (1) | WO2019113065A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2580017C2 (ru) | 2010-07-26 | 2016-04-10 | Трианни, Инк. | Трансгенные животные и способы применения |
| US10662256B2 (en) | 2010-07-26 | 2020-05-26 | Trianni, Inc. | Transgenic mammals and methods of use thereof |
| JP6444321B2 (ja) | 2013-02-22 | 2018-12-26 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | ヒト化主要組織適合性遺伝子複合体を発現するマウス |
| WO2017095939A1 (en) | 2015-12-03 | 2017-06-08 | Trianni, Inc. | Enhanced immunoglobulin diversity |
| PL3720279T3 (pl) | 2017-12-05 | 2023-01-16 | Regeneron Pharmaceuticals, Inc. | Myszy posiadające zmodyfikowany sposobami inżynierii łańcuch lekki lambda immunoglobuliny oraz ich zastosowania |
| IL318469A (en) | 2018-06-14 | 2025-03-01 | Regeneron Pharma | Non-human animals capable of reorganizing transgenic DH-DH, and their uses |
| EP3927153A1 (en) | 2019-02-22 | 2021-12-29 | Regeneron Pharmaceuticals, Inc. | Rodents having genetically modified sodium channels and methods of use thereof |
| JP7752536B2 (ja) * | 2019-07-01 | 2025-10-10 | トリアニ・インコーポレイテッド | トランスジェニック哺乳動物およびその使用法 |
| AU2020299569A1 (en) * | 2019-07-01 | 2022-01-20 | Trianni, Inc. | Transgenic mammals and methods of use |
| EP4211155A1 (en) | 2020-09-11 | 2023-07-19 | Regeneron Pharmaceuticals, Inc. | Identification and production of antigen-specific antibodies |
| EP4262373A1 (en) | 2020-12-16 | 2023-10-25 | Regeneron Pharmaceuticals, Inc. | Mice expressing humanized fc alpha receptors |
| JP7758739B2 (ja) | 2020-12-23 | 2025-10-22 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | アンカー改変型抗体をコードする核酸およびその使用 |
| AU2021409906A1 (en) | 2020-12-23 | 2023-06-15 | Regeneron Pharmaceuticals, Inc. | Methods for obtaining antibodies that bind transmembrane proteins and cells that produce the same |
| CN114763558B (zh) * | 2021-01-14 | 2024-02-20 | 上海泰槿生物技术有限公司 | 重组基因组、非人哺乳动物细胞及其产生方法和用途 |
| IL307238A (en) * | 2021-03-31 | 2023-11-01 | Regeneron Pharma | Transgenic mice containing human components of the cellular immune system with an enhanced diversity of the TCRB repertoire |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6300129B1 (en) | 1990-08-29 | 2001-10-09 | Genpharm International | Transgenic non-human animals for producing heterologous antibodies |
| AU669124B2 (en) * | 1991-09-18 | 1996-05-30 | Kyowa Hakko Kirin Co., Ltd. | Process for producing humanized chimera antibody |
| US5670356A (en) | 1994-12-12 | 1997-09-23 | Promega Corporation | Modified luciferase |
| US5874304A (en) | 1996-01-18 | 1999-02-23 | University Of Florida Research Foundation, Inc. | Humanized green fluorescent protein genes and methods |
| DE69738539T2 (de) * | 1996-12-03 | 2009-03-26 | Amgen Fremont Inc. | Vollkommen humane Antikörper die EGFR binden |
| GB9823930D0 (en) | 1998-11-03 | 1998-12-30 | Babraham Inst | Murine expression of human ig\ locus |
| CA2634294A1 (en) * | 2000-08-03 | 2002-02-14 | Therapeutic Human Polyclonals, Inc. | Production of humanized antibodies in transgenic animals |
| US6596541B2 (en) | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| US7105348B2 (en) | 2000-10-31 | 2006-09-12 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| US6586251B2 (en) | 2000-10-31 | 2003-07-01 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| US7435871B2 (en) | 2001-11-30 | 2008-10-14 | Amgen Fremont Inc. | Transgenic animals bearing human Igλ light chain genes |
| CA2652976C (en) | 2006-06-02 | 2015-08-11 | Regeneron Pharmaceuticals, Inc. | High affinity antibodies to human il-6 receptor |
| EP2195336B1 (en) * | 2007-09-12 | 2015-01-21 | Probiodrug AG | Transgenic mice |
| RU2559524C2 (ru) * | 2008-06-27 | 2015-08-10 | Мерюс Б.В. | Продуцирующие антитела млекопитающие, не являющиеся человеком |
| LT2346994T (lt) | 2008-09-30 | 2022-03-10 | Ablexis, Llc | Knock-in pelė, skirta chimerinių antikūnų gamybai |
| EP3028565B1 (en) | 2009-07-08 | 2017-09-27 | Kymab Limited | Animal models and therapeutic molecules |
| US9445581B2 (en) * | 2012-03-28 | 2016-09-20 | Kymab Limited | Animal models and therapeutic molecules |
| RU2425880C2 (ru) | 2009-07-30 | 2011-08-10 | Учреждение Российской академии наук Институт общей генетики им. Н.И. Вавилова РАН | Способ получения трансгенных мышей |
| SI2480676T1 (sl) | 2010-06-22 | 2016-10-28 | Regeneron Pharmaceuticals, Inc. | Hibridna mišja lahka veriga |
| DK2813573T1 (da) | 2011-02-25 | 2015-01-12 | Regeneron Pharma | ADAM6 mus |
| PL3216871T3 (pl) | 2011-10-17 | 2022-03-21 | Regeneron Pharmaceuticals, Inc. | Ograniczony łańcuch ciężki immonoglobuliny pochodzącej od myszy |
| US9253965B2 (en) * | 2012-03-28 | 2016-02-09 | Kymab Limited | Animal models and therapeutic molecules |
| MX378871B (es) * | 2011-12-20 | 2025-03-10 | Regeneron Pharma | Ratones con cadena ligera humanizada. |
| PL2809150T3 (pl) * | 2012-02-01 | 2020-04-30 | Regeneron Pharmaceuticals, Inc. | Humanizowane myszy wykazujące ekspresję łańcuchów ciężkich zawierających domeny VL |
| EP2858487B1 (en) * | 2012-06-12 | 2019-10-23 | Regeneron Pharmaceuticals, Inc. | Humanized non-human animals with restricted immunoglobulin heavy chain loci |
| JP6475172B2 (ja) | 2013-02-20 | 2019-02-27 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | ラットの遺伝子組換え |
| EP3456831B1 (en) | 2013-04-16 | 2021-07-14 | Regeneron Pharmaceuticals, Inc. | Targeted modification of rat genome |
| WO2015049517A2 (en) * | 2013-10-01 | 2015-04-09 | Kymab Limited | Animal models and therapeutic molecules |
| MX384887B (es) | 2014-06-23 | 2025-03-14 | Regeneron Pharma | Ensamblaje de adn mediado por nucleasa. |
| CA3176380A1 (en) | 2014-11-21 | 2016-05-26 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modification using paired guide rnas |
| BR112017013104A2 (pt) | 2014-12-19 | 2018-05-15 | Regeneron Pharma | métodos para modificar um locus genômico alvo em uma célula, para intensificar a recombinação homóloga em um locus genômico alvo em uma célula e para produzir uma geração f0 de um animal não humano. |
| IL263160B2 (en) | 2016-06-03 | 2024-01-01 | Regeneron Pharma | Non-human animals expressing exogenous terminal deoxynucleotidyltransferase |
| GB201710984D0 (en) * | 2017-07-07 | 2017-08-23 | Kymab Ltd | Cells, vertebrates, populations & methods |
| PL3720279T3 (pl) | 2017-12-05 | 2023-01-16 | Regeneron Pharmaceuticals, Inc. | Myszy posiadające zmodyfikowany sposobami inżynierii łańcuch lekki lambda immunoglobuliny oraz ich zastosowania |
-
2018
- 2018-12-04 PL PL18821945.5T patent/PL3720279T3/pl unknown
- 2018-12-04 SM SM20220448T patent/SMT202200448T1/it unknown
- 2018-12-04 EP EP22188627.8A patent/EP4140297B1/en active Active
- 2018-12-04 AU AU2018381316A patent/AU2018381316B2/en active Active
- 2018-12-04 KR KR1020257002291A patent/KR20250022220A/ko active Pending
- 2018-12-04 ES ES18821945T patent/ES2928995T3/es active Active
- 2018-12-04 EP EP25169830.4A patent/EP4596688A3/en active Pending
- 2018-12-04 IL IL318197A patent/IL318197A/en unknown
- 2018-12-04 HR HRP20221459TT patent/HRP20221459T1/hr unknown
- 2018-12-04 CN CN202211211765.0A patent/CN116058333A/zh active Pending
- 2018-12-04 BR BR112020011184-8A patent/BR112020011184A2/pt unknown
- 2018-12-04 RS RS20250752A patent/RS67058B1/sr unknown
- 2018-12-04 KR KR1020207016646A patent/KR102760506B1/ko active Active
- 2018-12-04 DK DK22188627.8T patent/DK4140297T3/da active
- 2018-12-04 SI SI201831243T patent/SI4140297T1/sl unknown
- 2018-12-04 ES ES22188627T patent/ES3035461T3/es active Active
- 2018-12-04 EP EP18821945.5A patent/EP3720279B1/en active Active
- 2018-12-04 CN CN201880078631.1A patent/CN111432635B/zh active Active
- 2018-12-04 HR HRP20250920TT patent/HRP20250920T1/hr unknown
- 2018-12-04 PT PT221886278T patent/PT4140297T/pt unknown
- 2018-12-04 HU HUE18821945A patent/HUE060608T2/hu unknown
- 2018-12-04 DK DK18821945.5T patent/DK3720279T3/da active
- 2018-12-04 MX MX2020005830A patent/MX2020005830A/es unknown
- 2018-12-04 LT LTEP22188627.8T patent/LT4140297T/lt unknown
- 2018-12-04 CA CA3084049A patent/CA3084049A1/en active Pending
- 2018-12-04 RS RS20221094A patent/RS63772B1/sr unknown
- 2018-12-04 SM SM20250277T patent/SMT202500277T1/it unknown
- 2018-12-04 IL IL274797A patent/IL274797B2/en unknown
- 2018-12-04 LT LTEPPCT/US2018/063841T patent/LT3720279T/lt unknown
- 2018-12-04 US US16/209,820 patent/US11051498B2/en active Active
- 2018-12-04 SI SI201830769T patent/SI3720279T1/sl unknown
- 2018-12-04 SG SG11202003044SA patent/SG11202003044SA/en unknown
- 2018-12-04 WO PCT/US2018/063841 patent/WO2019113065A1/en not_active Ceased
- 2018-12-04 TW TW107143491A patent/TWI827567B/zh active
- 2018-12-04 US US16/769,821 patent/US20210345591A1/en not_active Abandoned
- 2018-12-04 JP JP2020530565A patent/JP7430636B2/ja active Active
- 2018-12-04 TW TW112148224A patent/TW202438676A/zh unknown
- 2018-12-04 PT PT188219455T patent/PT3720279T/pt unknown
- 2018-12-04 PL PL22188627.8T patent/PL4140297T3/pl unknown
- 2018-12-04 HU HUE22188627A patent/HUE071852T2/hu unknown
- 2018-12-04 FI FIEP22188627.8T patent/FI4140297T3/fi active
-
2020
- 2020-07-13 MX MX2024004496A patent/MX2024004496A/es unknown
-
2021
- 2021-06-01 US US17/335,727 patent/US12356967B2/en active Active
-
2024
- 2024-01-31 JP JP2024012752A patent/JP7765514B2/ja active Active
-
2025
- 2025-06-18 AU AU2025204547A patent/AU2025204547A1/en active Pending
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