JP2020500528A5 - - Google Patents

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JP2020500528A5
JP2020500528A5 JP2019529497A JP2019529497A JP2020500528A5 JP 2020500528 A5 JP2020500528 A5 JP 2020500528A5 JP 2019529497 A JP2019529497 A JP 2019529497A JP 2019529497 A JP2019529497 A JP 2019529497A JP 2020500528 A5 JP2020500528 A5 JP 2020500528A5
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nanopore
sample
adapter
strand
tag
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JP7055805B2 (ja
JP2020500528A (ja
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JP2019529497A 2016-12-01 2017-11-29 ナノポアを用いて検体を特徴付けるための方法及びシステム Active JP7055805B2 (ja)

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Application Number Priority Date Filing Date Title
GBGB1620450.5A GB201620450D0 (en) 2016-12-01 2016-12-01 Method
GB1620450.5 2016-12-01
US201762471338P 2017-03-14 2017-03-14
US62/471,338 2017-03-14
PCT/GB2017/053603 WO2018100370A1 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores

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JP2020500528A JP2020500528A (ja) 2020-01-16
JP2020500528A5 true JP2020500528A5 (enExample) 2021-01-14
JP7055805B2 JP7055805B2 (ja) 2022-04-18

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US (2) US11466317B2 (enExample)
EP (2) EP4269617A3 (enExample)
JP (1) JP7055805B2 (enExample)
CN (3) CN110168104A (enExample)
AU (1) AU2017367238B2 (enExample)
CA (1) CA3044351A1 (enExample)
GB (1) GB201620450D0 (enExample)
WO (1) WO2018100370A1 (enExample)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
CN109196116B (zh) 2016-05-25 2022-09-27 牛津纳米孔科技公开有限公司 一种表征靶多核苷酸的方法
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
CN121472373A (zh) 2018-05-22 2026-02-06 安序源有限公司 用于核酸测序的方法、系统和组合物
US12269037B2 (en) 2018-05-24 2025-04-08 Oxford Nanopore Technologies Plc Droplet interfaces in electro-wetting devices
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB2585816A (en) * 2018-12-12 2021-01-27 Univ York Proof-of-work for blockchain applications
CN111455031A (zh) * 2019-01-18 2020-07-28 中国科学院微生物研究所 基于Nanopore测序技术的多组学测序及分析方法
US11789006B2 (en) * 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
US20230220450A1 (en) * 2019-09-18 2023-07-13 Hitachi High-Tech Corporation Adapter molecule, biomolecule-adapter molecule complex composed of adapter molecule and biomolecule bound together, biomolecule analyzer and biomolecule analysis method
CN115398007A (zh) * 2020-02-10 2022-11-25 帕洛根公司 纳米孔装置和使用其检测和分类带电粒子的方法
US20230003648A1 (en) * 2020-03-20 2023-01-05 GeneSense Technology Inc. High throughput analytical system for molecule detection and sensing
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
CN113930406B (zh) * 2021-12-17 2022-04-08 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
EP4176079A1 (en) * 2020-07-06 2023-05-10 Illumina, Inc. Separating polynucleotide fragments
CN112309503A (zh) * 2020-10-19 2021-02-02 深圳市儒翰基因科技有限公司 基于纳米孔电信号的碱基判读方法、判读设备及存储介质
US12090477B2 (en) * 2021-03-05 2024-09-17 Taiwan Semiconductor Manufacturing Company Ltd. System and method for detecting biomolecules
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
WO2022243692A1 (en) * 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
CN113322180B (zh) * 2021-05-31 2022-07-15 中国科学院物理研究所 基于纳米孔测序的力谱分析方法和分析装置
CN115537413A (zh) * 2021-06-29 2022-12-30 中国农业大学 一种双链功能核酸纳米花的组装和形貌调控方法及其应用
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
CN118318049A (zh) 2021-09-28 2024-07-09 安序源有限公司 用于处理核酸样品的方法以及核酸样品的组合物
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
EP4458983A4 (en) * 2021-12-30 2026-01-21 Bgi Shenzhen SINGLE MOLECULE NANOPORE SEQUENCING METHOD
EP4471141A4 (en) * 2021-12-31 2025-12-10 Bgi Shenzhen Nucleic acid molecule capable of blocking a motor protein, its construction process and its application
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
AU2023270700A1 (en) 2022-05-17 2024-10-31 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
WO2024077281A2 (en) * 2022-10-06 2024-04-11 Cornell University Genotyping and sequencing assays for prrsv
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
WO2024086745A2 (en) * 2022-10-21 2024-04-25 Axbio Inc. Systems and methods for analyzing a target molecule
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
EP4616408A1 (en) 2022-11-11 2025-09-17 UMC Utrecht Holding B.V. Computer-implemented method for identifying a dna modification
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
CN120359304A (zh) * 2022-12-16 2025-07-22 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
WO2025000432A1 (zh) * 2023-06-30 2025-01-02 深圳华大生命科学研究院 利用封闭子进行纳米孔测序的方法
WO2025223677A1 (en) * 2024-04-26 2025-10-30 Helmholtz-Zentrum für Infektionsforschung GmbH Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
AU2206800A (en) 1998-12-11 2000-06-26 Regents Of The University Of California, The Targeted molecular bar codes and methods for using the same
US6916665B2 (en) * 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
FR2814939B1 (fr) 2000-10-05 2002-12-20 Sofradim Production Ensemble de soutenement sous-uretral dans le traitement de l'incontinence urinaire d'effort de la femme
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
BRPI1007215A2 (pt) 2009-01-30 2017-08-29 Oxford Nanopore Tech Ltd Método de acoplamento covalente de duas ou mais porções, primeira e segunda porções, primeiro porção acoplada a uma segunda porção, par de primeiro e segundo ligantes, e, uso de um par de ligantes.
GB0901588D0 (en) 2009-02-02 2009-03-11 Itis Holdings Plc Apparatus and methods for providing journey information
CA2781581C (en) 2009-12-01 2018-03-06 Oxford Nanopore Technologies Limited Biochemical analysis instrument
WO2012003330A2 (en) 2010-06-30 2012-01-05 Stratos Genomics, Inc Multiplexed identification of nucleic acid sequences
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
EP3848706B1 (en) * 2011-05-27 2023-07-19 Oxford Nanopore Technologies PLC Coupling method
BR112014001699A2 (pt) 2011-07-25 2017-06-13 Oxford Nanopore Tech Ltd método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
EP2798084B1 (en) 2011-12-29 2017-04-19 Oxford Nanopore Technologies Limited Enzyme method
WO2013109970A1 (en) 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
EP3363901B1 (en) * 2012-02-17 2020-12-30 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
GB2557509A (en) 2012-06-08 2018-06-20 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
US20150119259A1 (en) * 2012-06-20 2015-04-30 Jingyue Ju Nucleic acid sequencing by nanopore detection of tag molecules
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
WO2014013262A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
WO2014064444A1 (en) 2012-10-26 2014-05-01 Oxford Nanopore Technologies Limited Droplet interfaces
CA2890515C (en) * 2012-11-09 2021-11-09 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
CN203450281U (zh) 2013-08-01 2014-02-26 大行科技(深圳)有限公司 一种折叠自行车走线结构
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10047392B2 (en) 2014-02-21 2018-08-14 Northeastern University Fluorescence-based analysis of biopolymers using nanopores
US20170096703A1 (en) 2014-03-21 2017-04-06 Oxford Nanopore Technologies Ltd. Analysis of a polymer from multi-dimensional measurements
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3143165A4 (en) 2014-05-15 2018-05-16 Two Pore Guys, Inc. Scaffold data storage and target detection in a sample using a nanopore
CA2950298C (en) 2014-06-03 2024-01-09 Illumina, Inc. Compositions, systems, and methods for detecting events using tethers anchored to or adjacent to nanopores
WO2016034591A2 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
KR102457147B1 (ko) * 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
US9557294B2 (en) * 2014-12-19 2017-01-31 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
EP3259372B1 (en) 2015-02-17 2020-11-18 MGI Tech Co., Ltd. Dna sequencing using controlled strand displacement
WO2016161402A1 (en) 2015-04-03 2016-10-06 Abbott Laboratories Devices and methods for sample analysis
KR102782598B1 (ko) 2015-06-25 2025-03-14 로스웰 엠이 아이엔씨. 바이오분자 센서들 및 방법들
WO2017125565A1 (en) 2016-01-21 2017-07-27 Genia Technologies, Inc. Nanopore sequencing complexes
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method

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