CN116334200B - 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物 - Google Patents

使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物 Download PDF

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CN116334200B
CN116334200B CN202310379929.9A CN202310379929A CN116334200B CN 116334200 B CN116334200 B CN 116334200B CN 202310379929 A CN202310379929 A CN 202310379929A CN 116334200 B CN116334200 B CN 116334200B
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polynucleotide
nanopore
complementary
strand
stranded
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CN116334200A (zh
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詹姆斯·克拉克
詹姆斯·怀特
理查德·马斯喀特
杰西卡·玛丽·梅·克诺特
拉米兹·伊克巴尔·纳撒尼
安德鲁·约翰·赫伦
马克·约翰·布鲁斯
拉科马·尼尚萨·贾亚辛格
多梅尼科·卡普罗蒂
戴维·杰克逊·斯托达特
瑞贝卡·维多利亚·鲍恩
克里斯多夫·詹姆斯·莱特
保罗·理查德·穆迪
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Oxford Nanopore Technology Public Co ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

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US62/471,338 2017-03-14
CN201780083105.XA CN110168104A (zh) 2016-12-01 2017-11-29 使用纳米孔表征分析物的方法和系统
PCT/GB2017/053603 WO2018100370A1 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores
CN202310379929.9A CN116334200B (zh) 2016-12-01 2017-11-29 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物

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Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3207154B1 (en) 2014-10-16 2022-03-16 Oxford Nanopore Technologies PLC Analysis of a polymer
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN109196116B (zh) 2016-05-25 2022-09-27 牛津纳米孔科技公开有限公司 一种表征靶多核苷酸的方法
GB201620450D0 (en) * 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
CN112534063A (zh) 2018-05-22 2021-03-19 安序源有限公司 用于核酸测序的方法、系统和组合物
EP3801901A1 (en) 2018-05-24 2021-04-14 Oxford Nanopore Technologies Limited Droplet interfaces in electro-wetting devices
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB2585816A (en) * 2018-12-12 2021-01-27 Univ York Proof-of-work for blockchain applications
CN111455031A (zh) * 2019-01-18 2020-07-28 中国科学院微生物研究所 基于Nanopore测序技术的多组学测序及分析方法
JP7492200B2 (ja) * 2019-03-12 2024-05-29 オックスフォード ナノポール テクノロジーズ ピーエルシー ナノ細孔センシングデバイスと操作方法およびその成形方法
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
CN114364797B (zh) * 2019-09-18 2023-10-20 株式会社日立高新技术 生物分子分析方法
KR20220148196A (ko) * 2020-02-10 2022-11-04 팔로젠 인코포레이티드 나노포어 디바이스 및 이를 사용하여 하전된 입자들을 검출하고 분류하는 방법
WO2021184374A1 (en) * 2020-03-20 2021-09-23 Genesense Technology Inc High throughput analytical system for molecule detection and sensing
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
CN113930406B (zh) * 2021-12-17 2022-04-08 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
US11760994B2 (en) * 2020-07-06 2023-09-19 Illumina, Inc. Separating polynucleotide fragments
CN112309503A (zh) * 2020-10-19 2021-02-02 深圳市儒翰基因科技有限公司 基于纳米孔电信号的碱基判读方法、判读设备及存储介质
US12090477B2 (en) * 2021-03-05 2024-09-17 Taiwan Semiconductor Manufacturing Company Ltd. System and method for detecting biomolecules
EP4341433A1 (en) * 2021-05-19 2024-03-27 Oxford Nanopore Technologies PLC Methods for complement strand sequencing
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
CN113322180B (zh) * 2021-05-31 2022-07-15 中国科学院物理研究所 基于纳米孔测序的力谱分析方法和分析装置
CN115537413A (zh) * 2021-06-29 2022-12-30 中国农业大学 一种双链功能核酸纳米花的组装和形貌调控方法及其应用
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
CN118318049A (zh) 2021-09-28 2024-07-09 安序源有限公司 用于处理核酸样品的方法以及核酸样品的组合物
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
WO2023125605A1 (zh) * 2021-12-30 2023-07-06 深圳华大生命科学研究院 单分子纳米孔测序方法
US20250136974A1 (en) * 2021-12-31 2025-05-01 Bgi Shenzhen Nucleic acid molecule capable of blocking motor protein, and construction method and application thereof
US12559791B2 (en) 2022-05-06 2026-02-24 10X Genomics, Inc. Methods and compositions for in situ analysis of V(D)J sequences
AU2023270700A1 (en) 2022-05-17 2024-10-31 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
WO2024077281A2 (en) * 2022-10-06 2024-04-11 Cornell University Genotyping and sequencing assays for prrsv
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
EP4605552A2 (en) * 2022-10-21 2025-08-27 Axbio Inc. Systems and methods for analyzing a target molecule
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024101988A1 (en) 2022-11-11 2024-05-16 Umc Utrecht Holding B.V. Computer-implemented method for identifying a dna modification
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
CN120359304A (zh) * 2022-12-16 2025-07-22 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
WO2025000432A1 (zh) * 2023-06-30 2025-01-02 深圳华大生命科学研究院 利用封闭子进行纳米孔测序的方法
WO2025223677A1 (en) * 2024-04-26 2025-10-30 Helmholtz-Zentrum für Infektionsforschung GmbH Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method
WO2026057755A1 (en) 2024-09-13 2026-03-19 Oxford Nanopore Technologies Plc Adaptors and kits for rna molecules labelling and characterising

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2016099673A1 (en) * 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
EP2083015B1 (en) * 2000-02-11 2016-04-06 The Texas A & M University System Biosensor compositions and methods of use
FR2814939B1 (fr) 2000-10-05 2002-12-20 Sofradim Production Ensemble de soutenement sous-uretral dans le traitement de l'incontinence urinaire d'effort de la femme
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US8278055B2 (en) * 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US20100196203A1 (en) 2007-02-20 2010-08-05 Gurdial Singh Sanghera Formation of Lipid Bilayers
US8698481B2 (en) 2007-09-12 2014-04-15 President And Fellows Of Harvard College High-resolution molecular sensor
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2010004273A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
GB0901588D0 (en) 2009-02-02 2009-03-11 Itis Holdings Plc Apparatus and methods for providing journey information
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
US8586301B2 (en) 2010-06-30 2013-11-19 Stratos Genomics, Inc. Multiplexed identification of nucleic acid sequences
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
EP2673638B1 (en) 2011-02-11 2019-10-30 Oxford Nanopore Technologies Limited Mutant pores
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
EP3633370B1 (en) * 2011-05-27 2024-05-01 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
IN2014DN00221A (enExample) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
CA3113287C (en) 2011-09-23 2022-12-20 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
WO2013098561A1 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
WO2013109970A1 (en) * 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
HUE053360T2 (hu) * 2012-02-17 2021-06-28 Hutchinson Fred Cancer Res Kompozíciók és eljárások mutációk pontos azonosítására
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
CA2872141C (en) 2012-05-31 2016-01-19 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
EP2864502B1 (en) * 2012-06-20 2019-10-23 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
CA2889664C (en) 2012-10-26 2020-12-29 Oxford Nanopore Technologies Limited Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
EP2917732B1 (en) * 2012-11-09 2016-09-07 Stratos Genomics Inc. Concentrating a target molecule for sensing by a nanopore
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
BR112015021788B1 (pt) 2013-03-08 2023-02-28 Oxford Nanopore Technologies Plc Métodos para mover uma ou mais helicases imobilizadas, para controlar o movimento de um polinucleotídeo alvo, para caracterizar um polinucleotídeo alvo e para controlar o carregamento de uma ou mais helicases em um polinucleotídeo alvo, uso de um poro de transmembrana e de um potencial aplicado e de um ou mais espaçadores, complexo, e, kit
CN105102627B (zh) 2013-03-15 2018-10-19 纽约哥伦比亚大学理事会 用于检测样品中多种预定化合物的方法
CN203450281U (zh) 2013-08-01 2014-02-26 大行科技(深圳)有限公司 一种折叠自行车走线结构
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CA2937411C (en) 2014-01-22 2023-09-26 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3107865A4 (en) 2014-02-21 2017-10-18 Northeastern University Fluorescence-based analysis of biopolymers using nanopores
US20170096703A1 (en) 2014-03-21 2017-04-06 Oxford Nanopore Technologies Ltd. Analysis of a polymer from multi-dimensional measurements
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3137627B1 (en) 2014-05-02 2025-07-30 Oxford Nanopore Technologies plc Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US20170074855A1 (en) 2014-05-15 2017-03-16 Two Pore Guys, Inc. Scaffold Data Storage and Target Detection in a Sample Using a Nanopore
CN106687574B (zh) 2014-06-03 2021-06-29 亿明达股份有限公司 使用对纳米颗粒或纳米颗粒附近锚定的系链检测事件的组合物，系统和方法
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3207154B1 (en) 2014-10-16 2022-03-16 Oxford Nanopore Technologies PLC Analysis of a polymer
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
CN107250383B (zh) * 2015-02-17 2022-09-06 深圳华大智造科技股份有限公司 使用受控的链置换的dna序列测定
CN107690582B (zh) 2015-04-03 2023-10-20 雅培制药有限公司 用于样品分析的装置和方法
CA3021580A1 (en) 2015-06-25 2016-12-29 Barry L. Merriman Biomolecular sensors and methods
WO2017125565A1 (en) 2016-01-21 2017-07-27 Genia Technologies, Inc. Nanopore sequencing complexes
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2016099673A1 (en) * 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

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