CN116334200B - 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物 - Google Patents

使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物 Download PDF

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CN116334200B
CN116334200B CN202310379929.9A CN202310379929A CN116334200B CN 116334200 B CN116334200 B CN 116334200B CN 202310379929 A CN202310379929 A CN 202310379929A CN 116334200 B CN116334200 B CN 116334200B
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polynucleotide
nanopore
complementary
strand
stranded
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CN116334200A (zh
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詹姆斯·克拉克
詹姆斯·怀特
理查德·马斯喀特
杰西卡·玛丽·梅·克诺特
拉米兹·伊克巴尔·纳撒尼
安德鲁·约翰·赫伦
马克·约翰·布鲁斯
拉科马·尼尚萨·贾亚辛格
多梅尼科·卡普罗蒂
戴维·杰克逊·斯托达特
瑞贝卡·维多利亚·鲍恩
克里斯多夫·詹姆斯·莱特
保罗·理查德·穆迪
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Oxford Nanopore Technology Public Co ltd
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
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    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores

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US201762471338P 2017-03-14 2017-03-14
US62/471,338 2017-03-14
CN202310379929.9A CN116334200B (zh) 2016-12-01 2017-11-29 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物
CN201780083105.XA CN110168104A (zh) 2016-12-01 2017-11-29 使用纳米孔表征分析物的方法和系统
PCT/GB2017/053603 WO2018100370A1 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores

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Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
CA2879261C (en) 2012-07-19 2022-12-06 Oxford Nanopore Technologies Limited Modified helicases
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
CN109196116B (zh) 2016-05-25 2022-09-27 牛津纳米孔科技公开有限公司 一种表征靶多核苷酸的方法
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
CN112534063A (zh) 2018-05-22 2021-03-19 安序源有限公司 用于核酸测序的方法、系统和组合物
KR102796115B1 (ko) 2018-05-24 2025-04-14 옥스포드 나노포어 테크놀로지즈 피엘씨 전기 습윤 소자 내 액적 계면
GB201809323D0 (en) * 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB2585816A (en) * 2018-12-12 2021-01-27 Univ York Proof-of-work for blockchain applications
CN111455031A (zh) * 2019-01-18 2020-07-28 中国科学院微生物研究所 基于Nanopore测序技术的多组学测序及分析方法
JP7492200B2 (ja) * 2019-03-12 2024-05-29 オックスフォード ナノポール テクノロジーズ ピーエルシー ナノ細孔センシングデバイスと操作方法およびその成形方法
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
WO2021053745A1 (ja) * 2019-09-18 2021-03-25 株式会社日立ハイテク アダプター分子、当該アダプター分子と生体分子とが結合した生体分子-アダプター分子複合体、生体分子分析装置及び生体分子分析方法
US20210247378A1 (en) * 2020-02-10 2021-08-12 Palogen, Inc. Nanopore device and methods of detecting and classifying charged particles using same
WO2021184374A1 (en) * 2020-03-20 2021-09-23 Genesense Technology Inc High throughput analytical system for molecule detection and sensing
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
CN113930406B (zh) * 2021-12-17 2022-04-08 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
WO2022010662A1 (en) * 2020-07-06 2022-01-13 Illumina, Inc. Separating polynucleotide fragments
CN112309503A (zh) * 2020-10-19 2021-02-02 深圳市儒翰基因科技有限公司 基于纳米孔电信号的碱基判读方法、判读设备及存储介质
US12090477B2 (en) * 2021-03-05 2024-09-17 Taiwan Semiconductor Manufacturing Company Ltd. System and method for detecting biomolecules
EP4341433A1 (en) * 2021-05-19 2024-03-27 Oxford Nanopore Technologies PLC Methods for complement strand sequencing
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
CN113322180B (zh) * 2021-05-31 2022-07-15 中国科学院物理研究所 基于纳米孔测序的力谱分析方法和分析装置
CN115537413A (zh) * 2021-06-29 2022-12-30 中国农业大学 一种双链功能核酸纳米花的组装和形貌调控方法及其应用
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
CN118318049A (zh) 2021-09-28 2024-07-09 安序源有限公司 用于处理核酸样品的方法以及核酸样品的组合物
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN118591641A (zh) * 2021-12-30 2024-09-03 深圳华大生命科学研究院 单分子纳米孔测序方法
EP4471141A4 (en) * 2021-12-31 2025-12-10 Bgi Shenzhen Nucleic acid molecule capable of blocking a motor protein, its construction process and its application
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
JP2025517736A (ja) 2022-05-17 2025-06-10 オックスフォード ナノポール テクノロジーズ ピーエルシー 方法及びアダプター
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
WO2024077281A2 (en) * 2022-10-06 2024-04-11 Cornell University Genotyping and sequencing assays for prrsv
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
CN120418447A (zh) * 2022-10-21 2025-08-01 安序源有限公司 用于分析靶分子的系统和方法
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024101988A1 (en) 2022-11-11 2024-05-16 Umc Utrecht Holding B.V. Computer-implemented method for identifying a dna modification
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
WO2024124567A1 (zh) * 2022-12-16 2024-06-20 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
EP4737587A1 (en) * 2023-06-30 2026-05-06 BGI Hangzhou CycloneSEQ Technoloy Co., Ltd. Nanopore sequencing method using closer
WO2025223677A1 (en) * 2024-04-26 2025-10-30 Helmholtz-Zentrum für Infektionsforschung GmbH Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method
WO2026057755A1 (en) 2024-09-13 2026-03-19 Oxford Nanopore Technologies Plc Adaptors and kits for rna molecules labelling and characterising

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2016099673A1 (en) * 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
WO2001059453A2 (en) * 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
FR2814939B1 (fr) 2000-10-05 2002-12-20 Sofradim Production Ensemble de soutenement sous-uretral dans le traitement de l'incontinence urinaire d'effort de la femme
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US20110229877A1 (en) * 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
EP2310534B1 (en) 2008-07-07 2018-09-05 Oxford Nanopore Technologies Limited Base-detecting pore
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
KR20110125226A (ko) 2009-01-30 2011-11-18 옥스포드 나노포어 테크놀로지즈 리미티드 혼성화 링커
GB0901588D0 (en) 2009-02-02 2009-03-11 Itis Holdings Plc Apparatus and methods for providing journey information
US9127313B2 (en) 2009-12-01 2015-09-08 Oxford Nanopore Technologies Limited Biochemical analysis instrument
WO2012003330A2 (en) 2010-06-30 2012-01-05 Stratos Genomics, Inc Multiplexed identification of nucleic acid sequences
WO2012042226A2 (en) 2010-10-01 2012-04-05 Oxford Nanopore Technologies Limited Biochemical analysis apparatus and rotary valve
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
EP4737389A2 (en) * 2011-05-27 2026-05-06 Oxford Nanopore Technologies PLC Coupling method
JP6298404B2 (ja) * 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
AU2012360244B2 (en) 2011-12-29 2018-08-23 Oxford Nanopore Technologies Limited Enzyme method
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
CN107828877A (zh) 2012-01-20 2018-03-23 吉尼亚科技公司 基于纳米孔的分子检测与测序
WO2013123442A1 (en) * 2012-02-17 2013-08-22 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
EP3674412A1 (en) * 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
CA2879261C (en) 2012-07-19 2022-12-06 Oxford Nanopore Technologies Limited Modified helicases
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
WO2014074922A1 (en) * 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201314695D0 (en) * 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
CN203450281U (zh) 2013-08-01 2014-02-26 大行科技(深圳)有限公司 一种折叠自行车走线结构
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN111534504B (zh) 2014-01-22 2024-06-21 牛津纳米孔科技公开有限公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10047392B2 (en) 2014-02-21 2018-08-14 Northeastern University Fluorescence-based analysis of biopolymers using nanopores
WO2015140535A1 (en) 2014-03-21 2015-09-24 Oxford Nanopore Technologies Limited Analysis of a polymer from multi-dimensional measurements
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
WO2015176034A1 (en) 2014-05-15 2015-11-19 Two Pore Guys, Inc. Scaffold data storage and target detection in a sample using a nanopore
ES2846746T3 (es) 2014-06-03 2021-07-29 Illumina Inc Composiciones, sistemas y métodos para detectar acontecimientos usando fijaciones ancladas o adyacentes a nanoporos
CA2959220A1 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant csgg pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
US10480026B2 (en) 2014-10-17 2019-11-19 Oxford Nanopore Technologies Ltd. Method for nanopore RNA characterisation
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
JP6773687B2 (ja) * 2015-02-17 2020-10-21 エムジーアイ テック カンパニー リミテッドMGI Tech Co., Ltd. 制御された鎖置換を用いてのdna配列決定
EP3278108B1 (en) 2015-04-03 2021-03-17 Abbott Laboratories Devices and methods for sample analysis
CA3021580A1 (en) 2015-06-25 2016-12-29 Barry L. Merriman Biomolecular sensors and methods
US20200216887A1 (en) 2016-01-21 2020-07-09 Genia Technologies, Inc. Nanopore sequencing complexes
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2016099673A1 (en) * 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

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