JP7055805B2 - ナノポアを用いて検体を特徴付けるための方法及びシステム - Google Patents
ナノポアを用いて検体を特徴付けるための方法及びシステム Download PDFInfo
- Publication number
- JP7055805B2 JP7055805B2 JP2019529497A JP2019529497A JP7055805B2 JP 7055805 B2 JP7055805 B2 JP 7055805B2 JP 2019529497 A JP2019529497 A JP 2019529497A JP 2019529497 A JP2019529497 A JP 2019529497A JP 7055805 B2 JP7055805 B2 JP 7055805B2
- Authority
- JP
- Japan
- Prior art keywords
- polynucleotide
- nanopore
- strand
- adapter
- stranded
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Nanotechnology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1620450.5 | 2016-12-01 | ||
| GBGB1620450.5A GB201620450D0 (en) | 2016-12-01 | 2016-12-01 | Method |
| US201762471338P | 2017-03-14 | 2017-03-14 | |
| US62/471,338 | 2017-03-14 | ||
| PCT/GB2017/053603 WO2018100370A1 (en) | 2016-12-01 | 2017-11-29 | Methods and systems for characterizing analytes using nanopores |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020500528A JP2020500528A (ja) | 2020-01-16 |
| JP2020500528A5 JP2020500528A5 (enExample) | 2021-01-14 |
| JP7055805B2 true JP7055805B2 (ja) | 2022-04-18 |
Family
ID=58159754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019529497A Active JP7055805B2 (ja) | 2016-12-01 | 2017-11-29 | ナノポアを用いて検体を特徴付けるための方法及びシステム |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11466317B2 (enExample) |
| EP (2) | EP3548636B1 (enExample) |
| JP (1) | JP7055805B2 (enExample) |
| CN (3) | CN116334200B (enExample) |
| AU (1) | AU2017367238B2 (enExample) |
| CA (1) | CA3044351A1 (enExample) |
| GB (1) | GB201620450D0 (enExample) |
| WO (1) | WO2018100370A1 (enExample) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| CA2879261C (en) | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP4397972A3 (en) | 2014-10-16 | 2024-10-09 | Oxford Nanopore Technologies PLC | Alignment mapping estimation |
| CN109196116B (zh) | 2016-05-25 | 2022-09-27 | 牛津纳米孔科技公开有限公司 | 一种表征靶多核苷酸的方法 |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB2569630B (en) | 2017-12-21 | 2022-10-12 | Sharp Life Science Eu Ltd | Droplet Interfaces in Electro-wetting Devices |
| CN112534063A (zh) | 2018-05-22 | 2021-03-19 | 安序源有限公司 | 用于核酸测序的方法、系统和组合物 |
| KR102796115B1 (ko) | 2018-05-24 | 2025-04-14 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 전기 습윤 소자 내 액적 계면 |
| GB201809323D0 (en) * | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| GB2585816A (en) * | 2018-12-12 | 2021-01-27 | Univ York | Proof-of-work for blockchain applications |
| CN111455031A (zh) * | 2019-01-18 | 2020-07-28 | 中国科学院微生物研究所 | 基于Nanopore测序技术的多组学测序及分析方法 |
| JP7492200B2 (ja) * | 2019-03-12 | 2024-05-29 | オックスフォード ナノポール テクノロジーズ ピーエルシー | ナノ細孔センシングデバイスと操作方法およびその成形方法 |
| GB201907243D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Sensing interactions between molecular entities and nanapores |
| WO2021053745A1 (ja) * | 2019-09-18 | 2021-03-25 | 株式会社日立ハイテク | アダプター分子、当該アダプター分子と生体分子とが結合した生体分子-アダプター分子複合体、生体分子分析装置及び生体分子分析方法 |
| US20210247378A1 (en) * | 2020-02-10 | 2021-08-12 | Palogen, Inc. | Nanopore device and methods of detecting and classifying charged particles using same |
| WO2021184374A1 (en) * | 2020-03-20 | 2021-09-23 | Genesense Technology Inc | High throughput analytical system for molecule detection and sensing |
| US20230295712A1 (en) | 2020-06-18 | 2023-09-21 | Oxford Nanopore Technologies Plc | A method of selectively characterising a polynucleotide using a detector |
| CN113930406B (zh) * | 2021-12-17 | 2022-04-08 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| WO2022010662A1 (en) * | 2020-07-06 | 2022-01-13 | Illumina, Inc. | Separating polynucleotide fragments |
| CN112309503A (zh) * | 2020-10-19 | 2021-02-02 | 深圳市儒翰基因科技有限公司 | 基于纳米孔电信号的碱基判读方法、判读设备及存储介质 |
| US12090477B2 (en) * | 2021-03-05 | 2024-09-17 | Taiwan Semiconductor Manufacturing Company Ltd. | System and method for detecting biomolecules |
| EP4341433A1 (en) * | 2021-05-19 | 2024-03-27 | Oxford Nanopore Technologies PLC | Methods for complement strand sequencing |
| GB202107192D0 (en) * | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| CN113322180B (zh) * | 2021-05-31 | 2022-07-15 | 中国科学院物理研究所 | 基于纳米孔测序的力谱分析方法和分析装置 |
| CN115537413A (zh) * | 2021-06-29 | 2022-12-30 | 中国农业大学 | 一种双链功能核酸纳米花的组装和形貌调控方法及其应用 |
| CN113462764B (zh) * | 2021-09-01 | 2021-12-17 | 北京齐碳科技有限公司 | 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法 |
| CN118318049A (zh) | 2021-09-28 | 2024-07-09 | 安序源有限公司 | 用于处理核酸样品的方法以及核酸样品的组合物 |
| CN114134142B (zh) * | 2021-11-23 | 2024-05-28 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| CN118591641A (zh) * | 2021-12-30 | 2024-09-03 | 深圳华大生命科学研究院 | 单分子纳米孔测序方法 |
| EP4471141A4 (en) * | 2021-12-31 | 2025-12-10 | Bgi Shenzhen | Nucleic acid molecule capable of blocking a motor protein, its construction process and its application |
| WO2023215603A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| JP2025517736A (ja) | 2022-05-17 | 2025-06-10 | オックスフォード ナノポール テクノロジーズ ピーエルシー | 方法及びアダプター |
| GB202207267D0 (en) | 2022-05-18 | 2022-06-29 | Oxford Nanopore Tech Plc | Calibration and profiling of a nanopore array device |
| WO2024077281A2 (en) * | 2022-10-06 | 2024-04-11 | Cornell University | Genotyping and sequencing assays for prrsv |
| GB202215442D0 (en) | 2022-10-19 | 2022-11-30 | Oxford Nanopore Tech Plc | Analysis of a polymer |
| CN120418447A (zh) * | 2022-10-21 | 2025-08-01 | 安序源有限公司 | 用于分析靶分子的系统和方法 |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| WO2024101988A1 (en) | 2022-11-11 | 2024-05-16 | Umc Utrecht Holding B.V. | Computer-implemented method for identifying a dna modification |
| CN116478983B (zh) * | 2022-11-21 | 2023-10-24 | 北京普译生物科技有限公司 | 一种rna-dna嵌合接头及其应用 |
| WO2024124567A1 (zh) * | 2022-12-16 | 2024-06-20 | 深圳华大生命科学研究院 | 纳米孔测序方法和试剂盒 |
| GB202301095D0 (en) | 2023-01-25 | 2023-03-08 | Oxford Nanopore Tech Plc | Calibration of a nanopore array device |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
| GB202305524D0 (en) | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202307141D0 (en) | 2023-05-12 | 2023-06-28 | Oxford Nanopore Tech Plc | Modified helicases |
| EP4737587A1 (en) * | 2023-06-30 | 2026-05-06 | BGI Hangzhou CycloneSEQ Technoloy Co., Ltd. | Nanopore sequencing method using closer |
| WO2025223677A1 (en) * | 2024-04-26 | 2025-10-30 | Helmholtz-Zentrum für Infektionsforschung GmbH | Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method |
| WO2026057755A1 (en) | 2024-09-13 | 2026-03-19 | Oxford Nanopore Technologies Plc | Adaptors and kits for rna molecules labelling and characterising |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013185137A1 (en) | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| US20160041179A1 (en) | 2013-03-15 | 2016-02-11 | The Trustees Of Columbia University In The City Of New York | Method for detecting multiple predetermined compounds in a sample |
| WO2016099673A1 (en) | 2014-12-19 | 2016-06-23 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
Family Cites Families (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120160687A1 (en) | 1995-03-17 | 2012-06-28 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| WO2000034527A2 (en) | 1998-12-11 | 2000-06-15 | The Regents Of The University Of California | Targeted molecular bar codes |
| WO2001059453A2 (en) * | 2000-02-11 | 2001-08-16 | The Texas A & M University System | Biosensor compositions and methods of use |
| FR2814939B1 (fr) | 2000-10-05 | 2002-12-20 | Sofradim Production | Ensemble de soutenement sous-uretral dans le traitement de l'incontinence urinaire d'effort de la femme |
| US20020197618A1 (en) | 2001-01-20 | 2002-12-26 | Sampson Jeffrey R. | Synthesis and amplification of unstructured nucleic acids for rapid sequencing |
| US8278055B2 (en) | 2002-05-01 | 2012-10-02 | Intel Corporation | Methods and device for analyte characterization |
| US7238485B2 (en) | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| US20110121840A1 (en) | 2007-02-20 | 2011-05-26 | Gurdial Singh Sanghera | Lipid Bilayer Sensor System |
| WO2009035647A1 (en) | 2007-09-12 | 2009-03-19 | President And Fellows Of Harvard College | High-resolution molecular graphene sensor comprising an aperture in the graphene layer |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US20110229877A1 (en) * | 2008-07-07 | 2011-09-22 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| EP2310534B1 (en) | 2008-07-07 | 2018-09-05 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| GB0820927D0 (en) | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| KR20110125226A (ko) | 2009-01-30 | 2011-11-18 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 혼성화 링커 |
| GB0901588D0 (en) | 2009-02-02 | 2009-03-11 | Itis Holdings Plc | Apparatus and methods for providing journey information |
| US9127313B2 (en) | 2009-12-01 | 2015-09-08 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
| WO2012003330A2 (en) | 2010-06-30 | 2012-01-05 | Stratos Genomics, Inc | Multiplexed identification of nucleic acid sequences |
| WO2012042226A2 (en) | 2010-10-01 | 2012-04-05 | Oxford Nanopore Technologies Limited | Biochemical analysis apparatus and rotary valve |
| KR101939420B1 (ko) | 2011-02-11 | 2019-01-16 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 세공 |
| US9347929B2 (en) | 2011-03-01 | 2016-05-24 | The Regents Of The University Of Michigan | Controlling translocation through nanopores with fluid wall |
| EP4737389A2 (en) * | 2011-05-27 | 2026-05-06 | Oxford Nanopore Technologies PLC | Coupling method |
| JP6298404B2 (ja) * | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| CN107828877A (zh) | 2012-01-20 | 2018-03-23 | 吉尼亚科技公司 | 基于纳米孔的分子检测与测序 |
| WO2013123442A1 (en) * | 2012-02-17 | 2013-08-22 | Fred Hutchinson Cancer Research Center | Compositions and methods for accurately identifying mutations |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| AU2013267609C1 (en) | 2012-05-31 | 2019-01-03 | Board Of Regents, The University Of Texas System | Method for accurate sequencing of DNA |
| EP3674412A1 (en) * | 2012-06-20 | 2020-07-01 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
| JP6429773B2 (ja) | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| CA2879261C (en) | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| JP6375301B2 (ja) | 2012-10-26 | 2018-08-15 | オックスフォード ナノポール テクノロジーズ リミテッド | 液滴界面 |
| US9605309B2 (en) * | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| WO2014074922A1 (en) * | 2012-11-09 | 2014-05-15 | Stratos Genomics, Inc. | Concentrating a target molecule for sensing by a nanopore |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201314695D0 (en) * | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| CN203450281U (zh) | 2013-08-01 | 2014-02-26 | 大行科技(深圳)有限公司 | 一种折叠自行车走线结构 |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN111534504B (zh) | 2014-01-22 | 2024-06-21 | 牛津纳米孔科技公开有限公司 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| US10047392B2 (en) | 2014-02-21 | 2018-08-14 | Northeastern University | Fluorescence-based analysis of biopolymers using nanopores |
| WO2015140535A1 (en) | 2014-03-21 | 2015-09-24 | Oxford Nanopore Technologies Limited | Analysis of a polymer from multi-dimensional measurements |
| WO2015150786A1 (en) | 2014-04-04 | 2015-10-08 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP3137490B1 (en) | 2014-05-02 | 2021-01-27 | Oxford Nanopore Technologies Limited | Mutant pores |
| WO2015176034A1 (en) | 2014-05-15 | 2015-11-19 | Two Pore Guys, Inc. | Scaffold data storage and target detection in a sample using a nanopore |
| ES2846746T3 (es) | 2014-06-03 | 2021-07-29 | Illumina Inc | Composiciones, sistemas y métodos para detectar acontecimientos usando fijaciones ancladas o adyacentes a nanoporos |
| CA2959220A1 (en) | 2014-09-01 | 2016-03-10 | Vib Vzw | Mutant csgg pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| EP4397972A3 (en) | 2014-10-16 | 2024-10-09 | Oxford Nanopore Technologies PLC | Alignment mapping estimation |
| US10480026B2 (en) | 2014-10-17 | 2019-11-19 | Oxford Nanopore Technologies Ltd. | Method for nanopore RNA characterisation |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| JP6773687B2 (ja) * | 2015-02-17 | 2020-10-21 | エムジーアイ テック カンパニー リミテッドMGI Tech Co., Ltd. | 制御された鎖置換を用いてのdna配列決定 |
| EP3278108B1 (en) | 2015-04-03 | 2021-03-17 | Abbott Laboratories | Devices and methods for sample analysis |
| CA3021580A1 (en) | 2015-06-25 | 2016-12-29 | Barry L. Merriman | Biomolecular sensors and methods |
| US20200216887A1 (en) | 2016-01-21 | 2020-07-09 | Genia Technologies, Inc. | Nanopore sequencing complexes |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
-
2016
- 2016-12-01 GB GBGB1620450.5A patent/GB201620450D0/en not_active Ceased
-
2017
- 2017-11-29 CA CA3044351A patent/CA3044351A1/en active Pending
- 2017-11-29 AU AU2017367238A patent/AU2017367238B2/en active Active
- 2017-11-29 WO PCT/GB2017/053603 patent/WO2018100370A1/en not_active Ceased
- 2017-11-29 US US16/465,987 patent/US11466317B2/en active Active
- 2017-11-29 EP EP17817029.6A patent/EP3548636B1/en active Active
- 2017-11-29 JP JP2019529497A patent/JP7055805B2/ja active Active
- 2017-11-29 CN CN202310379929.9A patent/CN116334200B/zh active Active
- 2017-11-29 CN CN201780083105.XA patent/CN110168104A/zh active Pending
- 2017-11-29 EP EP23182828.6A patent/EP4269617A3/en active Pending
- 2017-11-29 CN CN202310379925.0A patent/CN116377044B/zh active Active
-
2022
- 2022-08-03 US US17/817,065 patent/US12509721B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013185137A1 (en) | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| US20160041179A1 (en) | 2013-03-15 | 2016-02-11 | The Trustees Of Columbia University In The City Of New York | Method for detecting multiple predetermined compounds in a sample |
| WO2016099673A1 (en) | 2014-12-19 | 2016-06-23 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3548636A1 (en) | 2019-10-09 |
| US20230084931A1 (en) | 2023-03-16 |
| US12509721B2 (en) | 2025-12-30 |
| EP4269617A2 (en) | 2023-11-01 |
| CN116377044B (zh) | 2025-05-06 |
| CA3044351A1 (en) | 2018-06-07 |
| US11466317B2 (en) | 2022-10-11 |
| AU2017367238A8 (en) | 2019-07-18 |
| CN116334200B (zh) | 2024-10-11 |
| EP4269617A3 (en) | 2024-01-10 |
| EP3548636B1 (en) | 2023-08-09 |
| AU2017367238B2 (en) | 2021-10-21 |
| US20190352709A1 (en) | 2019-11-21 |
| AU2017367238A1 (en) | 2019-06-13 |
| CN110168104A (zh) | 2019-08-23 |
| CN116334200A (zh) | 2023-06-27 |
| CN116377044A (zh) | 2023-07-04 |
| JP2020500528A (ja) | 2020-01-16 |
| GB201620450D0 (en) | 2017-01-18 |
| WO2018100370A1 (en) | 2018-06-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7055805B2 (ja) | ナノポアを用いて検体を特徴付けるための方法及びシステム | |
| EP3802877B1 (en) | Method | |
| US12584170B2 (en) | Method of nanopore sequencing of concatenated nucleic acids | |
| JP7065083B2 (ja) | ナノポアを通した誘導による核酸検出方法 | |
| CN114107457B (zh) | 向跨膜孔输送分析物的方法 | |
| US20250207188A1 (en) | Method | |
| EP3126516B1 (en) | Method of target molecule characterisation using a molecular pore | |
| JP2017503517A (ja) | 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20201127 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20201127 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20210929 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20211012 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20220107 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20220304 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20220329 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20220406 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 7055805 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |