CN110168104A - 使用纳米孔表征分析物的方法和系统 - Google Patents

使用纳米孔表征分析物的方法和系统 Download PDF

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Publication number
CN110168104A
CN110168104A CN201780083105.XA CN201780083105A CN110168104A CN 110168104 A CN110168104 A CN 110168104A CN 201780083105 A CN201780083105 A CN 201780083105A CN 110168104 A CN110168104 A CN 110168104A
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pore
polynucleotide
nano
stranded
polynucleotides
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Chinese (zh)
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詹姆斯·克拉克
詹姆斯·怀特
理查德·马斯喀特
杰西卡·玛丽·梅·克诺特
拉米兹·伊克巴尔·纳撒尼
安德鲁·约翰·赫伦
马克·约翰·布鲁斯
拉科马·尼尚萨·贾亚辛格
多梅尼科·卡普罗蒂
戴维·杰克逊·斯托达特
瑞贝卡·维多利亚·鲍恩
克里斯多夫·詹姆斯·莱特
保罗·理查德·穆迪
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Oxford Nanopore Technologies PLC
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Priority to CN202310379929.9A priority Critical patent/CN116334200B/zh
Priority to CN202310379925.0A priority patent/CN116377044B/zh
Publication of CN110168104A publication Critical patent/CN110168104A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores

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  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201780083105.XA 2016-12-01 2017-11-29 使用纳米孔表征分析物的方法和系统 Pending CN110168104A (zh)

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CN202310379929.9A CN116334200B (zh) 2016-12-01 2017-11-29 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物
CN202310379925.0A CN116377044B (zh) 2016-12-01 2017-11-29 用于使两个非共价结合的互补核酸链依次易位通过纳米孔的方法和复合物

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GBGB1620450.5A GB201620450D0 (en) 2016-12-01 2016-12-01 Method
GB1620450.5 2016-12-01
US201762471338P 2017-03-14 2017-03-14
US62/471,338 2017-03-14
PCT/GB2017/053603 WO2018100370A1 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores

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CN202310379925.0A Division CN116377044B (zh) 2016-12-01 2017-11-29 用于使两个非共价结合的互补核酸链依次易位通过纳米孔的方法和复合物

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CN202310379929.9A Active CN116334200B (zh) 2016-12-01 2017-11-29 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物

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CN202310379929.9A Active CN116334200B (zh) 2016-12-01 2017-11-29 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物

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EP (2) EP4269617A3 (enExample)
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AU (1) AU2017367238B2 (enExample)
CA (1) CA3044351A1 (enExample)
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112309503A (zh) * 2020-10-19 2021-02-02 深圳市儒翰基因科技有限公司 基于纳米孔电信号的碱基判读方法、判读设备及存储介质
CN113322180A (zh) * 2021-05-31 2021-08-31 中国科学院物理研究所 基于纳米孔测序的力谱分析方法和分析装置
CN113930406A (zh) * 2021-12-17 2022-01-14 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
CN114134142A (zh) * 2021-11-23 2022-03-04 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN114364797A (zh) * 2019-09-18 2022-04-15 株式会社日立高新技术 衔接子分子、该衔接子分子与生物分子结合而成的生物分子-衔接子分子复合体、生物分子分析装置和生物分子分析方法
CN114689672A (zh) * 2021-03-05 2022-07-01 台湾积体电路制造股份有限公司 用于检测生物分子的系统及方法
WO2023125605A1 (zh) * 2021-12-30 2023-07-06 深圳华大生命科学研究院 单分子纳米孔测序方法
CN116478983A (zh) * 2022-11-21 2023-07-25 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
WO2025000432A1 (zh) * 2023-06-30 2025-01-02 深圳华大生命科学研究院 利用封闭子进行纳米孔测序的方法

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
CN109196116B (zh) 2016-05-25 2022-09-27 牛津纳米孔科技公开有限公司 一种表征靶多核苷酸的方法
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
CN121472373A (zh) 2018-05-22 2026-02-06 安序源有限公司 用于核酸测序的方法、系统和组合物
US12269037B2 (en) 2018-05-24 2025-04-08 Oxford Nanopore Technologies Plc Droplet interfaces in electro-wetting devices
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB2585816A (en) * 2018-12-12 2021-01-27 Univ York Proof-of-work for blockchain applications
CN111455031A (zh) * 2019-01-18 2020-07-28 中国科学院微生物研究所 基于Nanopore测序技术的多组学测序及分析方法
US11789006B2 (en) * 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
CN115398007A (zh) * 2020-02-10 2022-11-25 帕洛根公司 纳米孔装置和使用其检测和分类带电粒子的方法
US20230003648A1 (en) * 2020-03-20 2023-01-05 GeneSense Technology Inc. High throughput analytical system for molecule detection and sensing
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
EP4176079A1 (en) * 2020-07-06 2023-05-10 Illumina, Inc. Separating polynucleotide fragments
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
WO2022243692A1 (en) * 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
CN115537413A (zh) * 2021-06-29 2022-12-30 中国农业大学 一种双链功能核酸纳米花的组装和形貌调控方法及其应用
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
CN118318049A (zh) 2021-09-28 2024-07-09 安序源有限公司 用于处理核酸样品的方法以及核酸样品的组合物
EP4471141A4 (en) * 2021-12-31 2025-12-10 Bgi Shenzhen Nucleic acid molecule capable of blocking a motor protein, its construction process and its application
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
AU2023270700A1 (en) 2022-05-17 2024-10-31 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
WO2024077281A2 (en) * 2022-10-06 2024-04-11 Cornell University Genotyping and sequencing assays for prrsv
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
WO2024086745A2 (en) * 2022-10-21 2024-04-25 Axbio Inc. Systems and methods for analyzing a target molecule
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
EP4616408A1 (en) 2022-11-11 2025-09-17 UMC Utrecht Holding B.V. Computer-implemented method for identifying a dna modification
CN120359304A (zh) * 2022-12-16 2025-07-22 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
WO2025223677A1 (en) * 2024-04-26 2025-10-30 Helmholtz-Zentrum für Infektionsforschung GmbH Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2016099673A1 (en) * 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
AU2206800A (en) 1998-12-11 2000-06-26 Regents Of The University Of California, The Targeted molecular bar codes and methods for using the same
US6916665B2 (en) * 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
FR2814939B1 (fr) 2000-10-05 2002-12-20 Sofradim Production Ensemble de soutenement sous-uretral dans le traitement de l'incontinence urinaire d'effort de la femme
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
BRPI1007215A2 (pt) 2009-01-30 2017-08-29 Oxford Nanopore Tech Ltd Método de acoplamento covalente de duas ou mais porções, primeira e segunda porções, primeiro porção acoplada a uma segunda porção, par de primeiro e segundo ligantes, e, uso de um par de ligantes.
GB0901588D0 (en) 2009-02-02 2009-03-11 Itis Holdings Plc Apparatus and methods for providing journey information
CA2781581C (en) 2009-12-01 2018-03-06 Oxford Nanopore Technologies Limited Biochemical analysis instrument
WO2012003330A2 (en) 2010-06-30 2012-01-05 Stratos Genomics, Inc Multiplexed identification of nucleic acid sequences
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
EP3848706B1 (en) * 2011-05-27 2023-07-19 Oxford Nanopore Technologies PLC Coupling method
BR112014001699A2 (pt) 2011-07-25 2017-06-13 Oxford Nanopore Tech Ltd método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
EP2798084B1 (en) 2011-12-29 2017-04-19 Oxford Nanopore Technologies Limited Enzyme method
WO2013109970A1 (en) 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
EP3363901B1 (en) * 2012-02-17 2020-12-30 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
US20150119259A1 (en) * 2012-06-20 2015-04-30 Jingyue Ju Nucleic acid sequencing by nanopore detection of tag molecules
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
WO2014013262A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
WO2014064444A1 (en) 2012-10-26 2014-05-01 Oxford Nanopore Technologies Limited Droplet interfaces
CA2890515C (en) * 2012-11-09 2021-11-09 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
CN203450281U (zh) 2013-08-01 2014-02-26 大行科技(深圳)有限公司 一种折叠自行车走线结构
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10047392B2 (en) 2014-02-21 2018-08-14 Northeastern University Fluorescence-based analysis of biopolymers using nanopores
US20170096703A1 (en) 2014-03-21 2017-04-06 Oxford Nanopore Technologies Ltd. Analysis of a polymer from multi-dimensional measurements
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3143165A4 (en) 2014-05-15 2018-05-16 Two Pore Guys, Inc. Scaffold data storage and target detection in a sample using a nanopore
CA2950298C (en) 2014-06-03 2024-01-09 Illumina, Inc. Compositions, systems, and methods for detecting events using tethers anchored to or adjacent to nanopores
WO2016034591A2 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
KR102457147B1 (ko) * 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
EP3259372B1 (en) 2015-02-17 2020-11-18 MGI Tech Co., Ltd. Dna sequencing using controlled strand displacement
WO2016161402A1 (en) 2015-04-03 2016-10-06 Abbott Laboratories Devices and methods for sample analysis
KR102782598B1 (ko) 2015-06-25 2025-03-14 로스웰 엠이 아이엔씨. 바이오분자 센서들 및 방법들
WO2017125565A1 (en) 2016-01-21 2017-07-27 Genia Technologies, Inc. Nanopore sequencing complexes
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2016099673A1 (en) * 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114364797B (zh) * 2019-09-18 2023-10-20 株式会社日立高新技术 生物分子分析方法
CN114364797A (zh) * 2019-09-18 2022-04-15 株式会社日立高新技术 衔接子分子、该衔接子分子与生物分子结合而成的生物分子-衔接子分子复合体、生物分子分析装置和生物分子分析方法
CN112309503A (zh) * 2020-10-19 2021-02-02 深圳市儒翰基因科技有限公司 基于纳米孔电信号的碱基判读方法、判读设备及存储介质
CN114689672A (zh) * 2021-03-05 2022-07-01 台湾积体电路制造股份有限公司 用于检测生物分子的系统及方法
CN114689672B (zh) * 2021-03-05 2024-05-07 台湾积体电路制造股份有限公司 用于检测生物分子的系统及方法
CN113322180A (zh) * 2021-05-31 2021-08-31 中国科学院物理研究所 基于纳米孔测序的力谱分析方法和分析装置
CN114134142A (zh) * 2021-11-23 2022-03-04 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
WO2023093688A1 (zh) * 2021-11-23 2023-06-01 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN113930406B (zh) * 2021-12-17 2022-04-08 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
CN113930406A (zh) * 2021-12-17 2022-01-14 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
WO2023125605A1 (zh) * 2021-12-30 2023-07-06 深圳华大生命科学研究院 单分子纳米孔测序方法
CN116478983A (zh) * 2022-11-21 2023-07-25 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
WO2025000432A1 (zh) * 2023-06-30 2025-01-02 深圳华大生命科学研究院 利用封闭子进行纳米孔测序的方法

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