CN110168104A - 使用纳米孔表征分析物的方法和系统 - Google Patents
使用纳米孔表征分析物的方法和系统 Download PDFInfo
- Publication number
- CN110168104A CN110168104A CN201780083105.XA CN201780083105A CN110168104A CN 110168104 A CN110168104 A CN 110168104A CN 201780083105 A CN201780083105 A CN 201780083105A CN 110168104 A CN110168104 A CN 110168104A
- Authority
- CN
- China
- Prior art keywords
- pore
- polynucleotide
- nano
- stranded
- polynucleotides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Nanotechnology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310379929.9A CN116334200B (zh) | 2016-12-01 | 2017-11-29 | 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物 |
| CN202310379925.0A CN116377044B (zh) | 2016-12-01 | 2017-11-29 | 用于使两个非共价结合的互补核酸链依次易位通过纳米孔的方法和复合物 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1620450.5A GB201620450D0 (en) | 2016-12-01 | 2016-12-01 | Method |
| GB1620450.5 | 2016-12-01 | ||
| US201762471338P | 2017-03-14 | 2017-03-14 | |
| US62/471,338 | 2017-03-14 | ||
| PCT/GB2017/053603 WO2018100370A1 (en) | 2016-12-01 | 2017-11-29 | Methods and systems for characterizing analytes using nanopores |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310379929.9A Division CN116334200B (zh) | 2016-12-01 | 2017-11-29 | 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物 |
| CN202310379925.0A Division CN116377044B (zh) | 2016-12-01 | 2017-11-29 | 用于使两个非共价结合的互补核酸链依次易位通过纳米孔的方法和复合物 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110168104A true CN110168104A (zh) | 2019-08-23 |
Family
ID=58159754
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780083105.XA Pending CN110168104A (zh) | 2016-12-01 | 2017-11-29 | 使用纳米孔表征分析物的方法和系统 |
| CN202310379925.0A Active CN116377044B (zh) | 2016-12-01 | 2017-11-29 | 用于使两个非共价结合的互补核酸链依次易位通过纳米孔的方法和复合物 |
| CN202310379929.9A Active CN116334200B (zh) | 2016-12-01 | 2017-11-29 | 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310379925.0A Active CN116377044B (zh) | 2016-12-01 | 2017-11-29 | 用于使两个非共价结合的互补核酸链依次易位通过纳米孔的方法和复合物 |
| CN202310379929.9A Active CN116334200B (zh) | 2016-12-01 | 2017-11-29 | 使用纳米孔确定多核苷酸特征的方法和用于该方法的复合物 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11466317B2 (enExample) |
| EP (2) | EP4269617A3 (enExample) |
| JP (1) | JP7055805B2 (enExample) |
| CN (3) | CN110168104A (enExample) |
| AU (1) | AU2017367238B2 (enExample) |
| CA (1) | CA3044351A1 (enExample) |
| GB (1) | GB201620450D0 (enExample) |
| WO (1) | WO2018100370A1 (enExample) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112309503A (zh) * | 2020-10-19 | 2021-02-02 | 深圳市儒翰基因科技有限公司 | 基于纳米孔电信号的碱基判读方法、判读设备及存储介质 |
| CN113322180A (zh) * | 2021-05-31 | 2021-08-31 | 中国科学院物理研究所 | 基于纳米孔测序的力谱分析方法和分析装置 |
| CN113930406A (zh) * | 2021-12-17 | 2022-01-14 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| CN114134142A (zh) * | 2021-11-23 | 2022-03-04 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| CN114364797A (zh) * | 2019-09-18 | 2022-04-15 | 株式会社日立高新技术 | 衔接子分子、该衔接子分子与生物分子结合而成的生物分子-衔接子分子复合体、生物分子分析装置和生物分子分析方法 |
| CN114689672A (zh) * | 2021-03-05 | 2022-07-01 | 台湾积体电路制造股份有限公司 | 用于检测生物分子的系统及方法 |
| WO2023125605A1 (zh) * | 2021-12-30 | 2023-07-06 | 深圳华大生命科学研究院 | 单分子纳米孔测序方法 |
| CN116478983A (zh) * | 2022-11-21 | 2023-07-25 | 北京普译生物科技有限公司 | 一种rna-dna嵌合接头及其应用 |
| WO2025000432A1 (zh) * | 2023-06-30 | 2025-01-02 | 深圳华大生命科学研究院 | 利用封闭子进行纳米孔测序的方法 |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140090633A (ko) | 2011-10-21 | 2014-07-17 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| JP6614972B2 (ja) | 2012-07-19 | 2019-12-04 | オックスフォード ナノポール テクノロジーズ リミテッド | 修飾ヘリカーゼ |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| CN118086476A (zh) | 2013-10-18 | 2024-05-28 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP4397972A3 (en) | 2014-10-16 | 2024-10-09 | Oxford Nanopore Technologies PLC | Alignment mapping estimation |
| CN109196116B (zh) | 2016-05-25 | 2022-09-27 | 牛津纳米孔科技公开有限公司 | 一种表征靶多核苷酸的方法 |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB2569630B (en) | 2017-12-21 | 2022-10-12 | Sharp Life Science Eu Ltd | Droplet Interfaces in Electro-wetting Devices |
| CN121472373A (zh) | 2018-05-22 | 2026-02-06 | 安序源有限公司 | 用于核酸测序的方法、系统和组合物 |
| US12269037B2 (en) | 2018-05-24 | 2025-04-08 | Oxford Nanopore Technologies Plc | Droplet interfaces in electro-wetting devices |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| GB2585816A (en) * | 2018-12-12 | 2021-01-27 | Univ York | Proof-of-work for blockchain applications |
| CN111455031A (zh) * | 2019-01-18 | 2020-07-28 | 中国科学院微生物研究所 | 基于Nanopore测序技术的多组学测序及分析方法 |
| US11789006B2 (en) * | 2019-03-12 | 2023-10-17 | Oxford Nanopore Technologies Plc | Nanopore sensing device, components and method of operation |
| GB201907243D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Sensing interactions between molecular entities and nanapores |
| CN115398007A (zh) * | 2020-02-10 | 2022-11-25 | 帕洛根公司 | 纳米孔装置和使用其检测和分类带电粒子的方法 |
| US20230003648A1 (en) * | 2020-03-20 | 2023-01-05 | GeneSense Technology Inc. | High throughput analytical system for molecule detection and sensing |
| US20230295712A1 (en) | 2020-06-18 | 2023-09-21 | Oxford Nanopore Technologies Plc | A method of selectively characterising a polynucleotide using a detector |
| EP4176079A1 (en) * | 2020-07-06 | 2023-05-10 | Illumina, Inc. | Separating polynucleotide fragments |
| GB202107192D0 (en) * | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| WO2022243692A1 (en) * | 2021-05-19 | 2022-11-24 | Oxford Nanopore Technologies Plc | Methods for complement strand sequencing |
| CN115537413A (zh) * | 2021-06-29 | 2022-12-30 | 中国农业大学 | 一种双链功能核酸纳米花的组装和形貌调控方法及其应用 |
| CN113462764B (zh) * | 2021-09-01 | 2021-12-17 | 北京齐碳科技有限公司 | 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法 |
| CN118318049A (zh) | 2021-09-28 | 2024-07-09 | 安序源有限公司 | 用于处理核酸样品的方法以及核酸样品的组合物 |
| EP4471141A4 (en) * | 2021-12-31 | 2025-12-10 | Bgi Shenzhen | Nucleic acid molecule capable of blocking a motor protein, its construction process and its application |
| WO2023215603A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| AU2023270700A1 (en) | 2022-05-17 | 2024-10-31 | Oxford Nanopore Technologies Plc | Method and adaptors |
| GB202207267D0 (en) | 2022-05-18 | 2022-06-29 | Oxford Nanopore Tech Plc | Calibration and profiling of a nanopore array device |
| WO2024077281A2 (en) * | 2022-10-06 | 2024-04-11 | Cornell University | Genotyping and sequencing assays for prrsv |
| GB202215442D0 (en) | 2022-10-19 | 2022-11-30 | Oxford Nanopore Tech Plc | Analysis of a polymer |
| WO2024086745A2 (en) * | 2022-10-21 | 2024-04-25 | Axbio Inc. | Systems and methods for analyzing a target molecule |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| EP4616408A1 (en) | 2022-11-11 | 2025-09-17 | UMC Utrecht Holding B.V. | Computer-implemented method for identifying a dna modification |
| CN120359304A (zh) * | 2022-12-16 | 2025-07-22 | 深圳华大生命科学研究院 | 纳米孔测序方法和试剂盒 |
| GB202301095D0 (en) | 2023-01-25 | 2023-03-08 | Oxford Nanopore Tech Plc | Calibration of a nanopore array device |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
| GB202305524D0 (en) | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202307141D0 (en) | 2023-05-12 | 2023-06-28 | Oxford Nanopore Tech Plc | Modified helicases |
| WO2025223677A1 (en) * | 2024-04-26 | 2025-10-30 | Helmholtz-Zentrum für Infektionsforschung GmbH | Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013185137A1 (en) * | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| WO2016099673A1 (en) * | 2014-12-19 | 2016-06-23 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
Family Cites Families (71)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US20120160687A1 (en) | 1995-03-17 | 2012-06-28 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| AU2206800A (en) | 1998-12-11 | 2000-06-26 | Regents Of The University Of California, The | Targeted molecular bar codes and methods for using the same |
| US6916665B2 (en) * | 2000-02-11 | 2005-07-12 | The Texas A&M University System | Biosensor compositions and methods of use |
| FR2814939B1 (fr) | 2000-10-05 | 2002-12-20 | Sofradim Production | Ensemble de soutenement sous-uretral dans le traitement de l'incontinence urinaire d'effort de la femme |
| US20020197618A1 (en) | 2001-01-20 | 2002-12-26 | Sampson Jeffrey R. | Synthesis and amplification of unstructured nucleic acids for rapid sequencing |
| US8278055B2 (en) | 2002-05-01 | 2012-10-02 | Intel Corporation | Methods and device for analyte characterization |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| EP2122344B8 (en) | 2007-02-20 | 2019-08-21 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor system |
| EP3540436B1 (en) | 2007-09-12 | 2023-11-01 | President And Fellows Of Harvard College | High-resolution molecular sensor |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| EP2307540B1 (en) | 2008-07-07 | 2017-04-19 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| JP2011527191A (ja) | 2008-07-07 | 2011-10-27 | オックスフォード ナノポア テクノロジーズ リミテッド | 塩基検出細孔 |
| GB0820927D0 (en) | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| BRPI1007215A2 (pt) | 2009-01-30 | 2017-08-29 | Oxford Nanopore Tech Ltd | Método de acoplamento covalente de duas ou mais porções, primeira e segunda porções, primeiro porção acoplada a uma segunda porção, par de primeiro e segundo ligantes, e, uso de um par de ligantes. |
| GB0901588D0 (en) | 2009-02-02 | 2009-03-11 | Itis Holdings Plc | Apparatus and methods for providing journey information |
| CA2781581C (en) | 2009-12-01 | 2018-03-06 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
| WO2012003330A2 (en) | 2010-06-30 | 2012-01-05 | Stratos Genomics, Inc | Multiplexed identification of nucleic acid sequences |
| US9593370B2 (en) | 2010-10-01 | 2017-03-14 | Oxford Nanopore Technologies Ltd. | Biochemical analysis apparatus and rotary valve |
| KR101939420B1 (ko) | 2011-02-11 | 2019-01-16 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 세공 |
| US9347929B2 (en) | 2011-03-01 | 2016-05-24 | The Regents Of The University Of Michigan | Controlling translocation through nanopores with fluid wall |
| EP3848706B1 (en) * | 2011-05-27 | 2023-07-19 | Oxford Nanopore Technologies PLC | Coupling method |
| BR112014001699A2 (pt) | 2011-07-25 | 2017-06-13 | Oxford Nanopore Tech Ltd | método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
| KR20140090633A (ko) | 2011-10-21 | 2014-07-17 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법 |
| EP2798083B1 (en) | 2011-12-29 | 2017-08-09 | Oxford Nanopore Technologies Limited | Method for characterising a polynucelotide by using a xpd helicase |
| EP2798084B1 (en) | 2011-12-29 | 2017-04-19 | Oxford Nanopore Technologies Limited | Enzyme method |
| WO2013109970A1 (en) | 2012-01-20 | 2013-07-25 | Genia Technologies, Inc. | Nanopore based molecular detection and sequencing |
| EP3363901B1 (en) * | 2012-02-17 | 2020-12-30 | Fred Hutchinson Cancer Research Center | Compositions and methods for accurately identifying mutations |
| CA2869546C (en) | 2012-04-10 | 2020-07-21 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| AU2013267609C1 (en) | 2012-05-31 | 2019-01-03 | Board Of Regents, The University Of Texas System | Method for accurate sequencing of DNA |
| US20150119259A1 (en) * | 2012-06-20 | 2015-04-30 | Jingyue Ju | Nucleic acid sequencing by nanopore detection of tag molecules |
| JP6614972B2 (ja) | 2012-07-19 | 2019-12-04 | オックスフォード ナノポール テクノロジーズ リミテッド | 修飾ヘリカーゼ |
| EP2875154B1 (en) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB method for characterising a nucleic acid |
| WO2014013262A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Enzyme construct |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| WO2014064444A1 (en) | 2012-10-26 | 2014-05-01 | Oxford Nanopore Technologies Limited | Droplet interfaces |
| CA2890515C (en) * | 2012-11-09 | 2021-11-09 | Stratos Genomics, Inc. | Concentrating a target molecule for sensing by a nanopore |
| US9605309B2 (en) * | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| AU2014224432B2 (en) | 2013-03-08 | 2019-10-24 | Oxford Nanopore Technologies Limited | Enzyme stalling method |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| US10732183B2 (en) | 2013-03-15 | 2020-08-04 | The Trustees Of Columbia University In The City Of New York | Method for detecting multiple predetermined compounds in a sample |
| CN203450281U (zh) | 2013-08-01 | 2014-02-26 | 大行科技(深圳)有限公司 | 一种折叠自行车走线结构 |
| CN118086476A (zh) | 2013-10-18 | 2024-05-28 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| WO2015110813A1 (en) | 2014-01-22 | 2015-07-30 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| US10047392B2 (en) | 2014-02-21 | 2018-08-14 | Northeastern University | Fluorescence-based analysis of biopolymers using nanopores |
| US20170096703A1 (en) | 2014-03-21 | 2017-04-06 | Oxford Nanopore Technologies Ltd. | Analysis of a polymer from multi-dimensional measurements |
| US10337060B2 (en) | 2014-04-04 | 2019-07-02 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| EP3137490B1 (en) | 2014-05-02 | 2021-01-27 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP3143165A4 (en) | 2014-05-15 | 2018-05-16 | Two Pore Guys, Inc. | Scaffold data storage and target detection in a sample using a nanopore |
| CA2950298C (en) | 2014-06-03 | 2024-01-09 | Illumina, Inc. | Compositions, systems, and methods for detecting events using tethers anchored to or adjacent to nanopores |
| WO2016034591A2 (en) | 2014-09-01 | 2016-03-10 | Vib Vzw | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| EP4397972A3 (en) | 2014-10-16 | 2024-10-09 | Oxford Nanopore Technologies PLC | Alignment mapping estimation |
| KR102457147B1 (ko) * | 2014-10-17 | 2022-10-19 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노 세공 rna 특징 규명을 위한 방법 |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| EP3259372B1 (en) | 2015-02-17 | 2020-11-18 | MGI Tech Co., Ltd. | Dna sequencing using controlled strand displacement |
| WO2016161402A1 (en) | 2015-04-03 | 2016-10-06 | Abbott Laboratories | Devices and methods for sample analysis |
| KR102782598B1 (ko) | 2015-06-25 | 2025-03-14 | 로스웰 엠이 아이엔씨. | 바이오분자 센서들 및 방법들 |
| WO2017125565A1 (en) | 2016-01-21 | 2017-07-27 | Genia Technologies, Inc. | Nanopore sequencing complexes |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
-
2016
- 2016-12-01 GB GBGB1620450.5A patent/GB201620450D0/en not_active Ceased
-
2017
- 2017-11-29 AU AU2017367238A patent/AU2017367238B2/en active Active
- 2017-11-29 CA CA3044351A patent/CA3044351A1/en active Pending
- 2017-11-29 JP JP2019529497A patent/JP7055805B2/ja active Active
- 2017-11-29 CN CN201780083105.XA patent/CN110168104A/zh active Pending
- 2017-11-29 CN CN202310379925.0A patent/CN116377044B/zh active Active
- 2017-11-29 EP EP23182828.6A patent/EP4269617A3/en active Pending
- 2017-11-29 CN CN202310379929.9A patent/CN116334200B/zh active Active
- 2017-11-29 EP EP17817029.6A patent/EP3548636B1/en active Active
- 2017-11-29 WO PCT/GB2017/053603 patent/WO2018100370A1/en not_active Ceased
- 2017-11-29 US US16/465,987 patent/US11466317B2/en active Active
-
2022
- 2022-08-03 US US17/817,065 patent/US12509721B2/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013185137A1 (en) * | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| WO2016099673A1 (en) * | 2014-12-19 | 2016-06-23 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114364797B (zh) * | 2019-09-18 | 2023-10-20 | 株式会社日立高新技术 | 生物分子分析方法 |
| CN114364797A (zh) * | 2019-09-18 | 2022-04-15 | 株式会社日立高新技术 | 衔接子分子、该衔接子分子与生物分子结合而成的生物分子-衔接子分子复合体、生物分子分析装置和生物分子分析方法 |
| CN112309503A (zh) * | 2020-10-19 | 2021-02-02 | 深圳市儒翰基因科技有限公司 | 基于纳米孔电信号的碱基判读方法、判读设备及存储介质 |
| CN114689672A (zh) * | 2021-03-05 | 2022-07-01 | 台湾积体电路制造股份有限公司 | 用于检测生物分子的系统及方法 |
| CN114689672B (zh) * | 2021-03-05 | 2024-05-07 | 台湾积体电路制造股份有限公司 | 用于检测生物分子的系统及方法 |
| CN113322180A (zh) * | 2021-05-31 | 2021-08-31 | 中国科学院物理研究所 | 基于纳米孔测序的力谱分析方法和分析装置 |
| CN114134142A (zh) * | 2021-11-23 | 2022-03-04 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| WO2023093688A1 (zh) * | 2021-11-23 | 2023-06-01 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| CN114134142B (zh) * | 2021-11-23 | 2024-05-28 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| CN113930406B (zh) * | 2021-12-17 | 2022-04-08 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| CN113930406A (zh) * | 2021-12-17 | 2022-01-14 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| WO2023125605A1 (zh) * | 2021-12-30 | 2023-07-06 | 深圳华大生命科学研究院 | 单分子纳米孔测序方法 |
| CN116478983A (zh) * | 2022-11-21 | 2023-07-25 | 北京普译生物科技有限公司 | 一种rna-dna嵌合接头及其应用 |
| CN116478983B (zh) * | 2022-11-21 | 2023-10-24 | 北京普译生物科技有限公司 | 一种rna-dna嵌合接头及其应用 |
| WO2025000432A1 (zh) * | 2023-06-30 | 2025-01-02 | 深圳华大生命科学研究院 | 利用封闭子进行纳米孔测序的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP7055805B2 (ja) | 2022-04-18 |
| AU2017367238B2 (en) | 2021-10-21 |
| GB201620450D0 (en) | 2017-01-18 |
| EP3548636A1 (en) | 2019-10-09 |
| US20230084931A1 (en) | 2023-03-16 |
| EP3548636B1 (en) | 2023-08-09 |
| CN116334200A (zh) | 2023-06-27 |
| EP4269617A2 (en) | 2023-11-01 |
| WO2018100370A1 (en) | 2018-06-07 |
| US20190352709A1 (en) | 2019-11-21 |
| CN116377044B (zh) | 2025-05-06 |
| AU2017367238A1 (en) | 2019-06-13 |
| US12509721B2 (en) | 2025-12-30 |
| CA3044351A1 (en) | 2018-06-07 |
| US11466317B2 (en) | 2022-10-11 |
| CN116377044A (zh) | 2023-07-04 |
| CN116334200B (zh) | 2024-10-11 |
| AU2017367238A8 (en) | 2019-07-18 |
| JP2020500528A (ja) | 2020-01-16 |
| EP4269617A3 (en) | 2024-01-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12509721B2 (en) | Methods and systems for characterizing analytes using nanopores | |
| US12571035B2 (en) | Method of target molecule characterisation using a molecular pore | |
| EP3802877B1 (en) | Method | |
| US11236385B2 (en) | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid | |
| EP3464624B1 (en) | Method of nanopore sequencing of concatenated nucleic acids | |
| CN107002151B (zh) | 向跨膜孔输送分析物的方法 | |
| CN109196116B (zh) | 一种表征靶多核苷酸的方法 | |
| CN110088299A (zh) | 通过纳米孔指导的核酸检测方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: oxford Applicant after: Oxford nanopore Technology Public Co.,Ltd. Address before: oxford Applicant before: OXFORD NANOPORE TECHNOLOGIES LTD. |