AU2017367238B2 - Methods and systems for characterizing analytes using nanopores - Google Patents

Methods and systems for characterizing analytes using nanopores Download PDF

Info

Publication number
AU2017367238B2
AU2017367238B2 AU2017367238A AU2017367238A AU2017367238B2 AU 2017367238 B2 AU2017367238 B2 AU 2017367238B2 AU 2017367238 A AU2017367238 A AU 2017367238A AU 2017367238 A AU2017367238 A AU 2017367238A AU 2017367238 B2 AU2017367238 B2 AU 2017367238B2
Authority
AU
Australia
Prior art keywords
polynucleotide
nanopore
strand
pore
adaptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2017367238A
Other languages
English (en)
Other versions
AU2017367238A8 (en
AU2017367238A1 (en
Inventor
Rebecca Victoria BOWEN
Mark John BRUCE
Domenico CAPROTTI
James Clarke
Andrew John Heron
Lakmal Nishantha JAYASINGHE
Jessica Mary May Knott
Paul Richard Moody
Richard Muscat
Ramiz Iqbal NATHANI
David Jackson STODDART
James White
Christopher James WRIGHT
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Publication of AU2017367238A1 publication Critical patent/AU2017367238A1/en
Publication of AU2017367238A8 publication Critical patent/AU2017367238A8/en
Application granted granted Critical
Publication of AU2017367238B2 publication Critical patent/AU2017367238B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2017367238A 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores Active AU2017367238B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1620450.5 2016-12-01
GBGB1620450.5A GB201620450D0 (en) 2016-12-01 2016-12-01 Method
US201762471338P 2017-03-14 2017-03-14
US62/471,338 2017-03-14
PCT/GB2017/053603 WO2018100370A1 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores

Publications (3)

Publication Number Publication Date
AU2017367238A1 AU2017367238A1 (en) 2019-06-13
AU2017367238A8 AU2017367238A8 (en) 2019-07-18
AU2017367238B2 true AU2017367238B2 (en) 2021-10-21

Family

ID=58159754

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017367238A Active AU2017367238B2 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores

Country Status (8)

Country Link
US (2) US11466317B2 (enExample)
EP (2) EP4269617A3 (enExample)
JP (1) JP7055805B2 (enExample)
CN (3) CN116377044B (enExample)
AU (1) AU2017367238B2 (enExample)
CA (1) CA3044351A1 (enExample)
GB (1) GB201620450D0 (enExample)
WO (1) WO2018100370A1 (enExample)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2017203268A1 (en) 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) * 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
WO2019226689A1 (en) 2018-05-22 2019-11-28 Axbio Inc. Methods, systems, and compositions for nucleic acid sequencing
CN112203767B (zh) 2018-05-24 2023-04-11 牛津纳米孔科技公司 电润湿装置中的液滴界面
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB2585816A (en) * 2018-12-12 2021-01-27 Univ York Proof-of-work for blockchain applications
CN111455031A (zh) * 2019-01-18 2020-07-28 中国科学院微生物研究所 基于Nanopore测序技术的多组学测序及分析方法
KR20210138594A (ko) * 2019-03-12 2021-11-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
GB2603061A (en) * 2019-09-18 2022-07-27 Hitachi High Tech Corp Adapter molecule, biomolecule-adapter molecule complex composed of adapter molecule and biomolecule bound together, biomolecule analyzer and biomolecule
WO2021163163A1 (en) * 2020-02-10 2021-08-19 Palogen, Inc. Nanopore device and methods of detecting and classifying charged particles using same
EP3991211A4 (en) * 2020-03-20 2022-07-20 GeneSense Technology Inc. High throughput analytical system for molecule detection and sensing
CN115698331A (zh) 2020-06-18 2023-02-03 牛津纳米孔科技公开有限公司 使用检测器选择性地表征多核苷酸的方法
CN113930406B (zh) * 2021-12-17 2022-04-08 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
MX2022014721A (es) * 2020-07-06 2023-03-06 Illumina Inc Separacion de fragmentos de polinucleotidos.
CN112309503A (zh) * 2020-10-19 2021-02-02 深圳市儒翰基因科技有限公司 基于纳米孔电信号的碱基判读方法、判读设备及存储介质
US12090477B2 (en) * 2021-03-05 2024-09-17 Taiwan Semiconductor Manufacturing Company Ltd. System and method for detecting biomolecules
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
CN117337333A (zh) 2021-05-19 2024-01-02 牛津纳米孔科技公开有限公司 用于补体链测序的方法
CN113322180B (zh) * 2021-05-31 2022-07-15 中国科学院物理研究所 基于纳米孔测序的力谱分析方法和分析装置
CN115537413A (zh) * 2021-06-29 2022-12-30 中国农业大学 一种双链功能核酸纳米花的组装和形貌调控方法及其应用
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
WO2023055752A2 (en) 2021-09-28 2023-04-06 Axbio Inc. Methods for processing a nucleic acid sample and compositions thereof
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
WO2023125605A1 (zh) * 2021-12-30 2023-07-06 深圳华大生命科学研究院 单分子纳米孔测序方法
US20250136974A1 (en) * 2021-12-31 2025-05-01 Bgi Shenzhen Nucleic acid molecule capable of blocking motor protein, and construction method and application thereof
EP4519453A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
WO2024077281A2 (en) * 2022-10-06 2024-04-11 Cornell University Genotyping and sequencing assays for prrsv
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
EP4605552A2 (en) * 2022-10-21 2025-08-27 Axbio Inc. Systems and methods for analyzing a target molecule
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024101988A1 (en) 2022-11-11 2024-05-16 Umc Utrecht Holding B.V. Computer-implemented method for identifying a dna modification
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
EP4636094A1 (en) * 2022-12-16 2025-10-22 BGI Shenzhen Nanopore sequencing method and kit
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
WO2025000432A1 (zh) * 2023-06-30 2025-01-02 深圳华大生命科学研究院 利用封闭子进行纳米孔测序的方法
WO2025223677A1 (en) * 2024-04-26 2025-10-30 Helmholtz-Zentrum für Infektionsforschung GmbH Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2016099673A1 (en) * 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
AU2001241474A1 (en) * 2000-02-11 2001-08-20 The Texas A And M University System Biosensor compositions and methods of use
FR2814939B1 (fr) 2000-10-05 2002-12-20 Sofradim Production Ensemble de soutenement sous-uretral dans le traitement de l'incontinence urinaire d'effort de la femme
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
NZ579083A (en) 2007-02-20 2012-07-27 Oxford Nanopore Tech Ltd Lipid bilayer sensor system
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US20110229877A1 (en) * 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CA2750874A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
GB0901588D0 (en) 2009-02-02 2009-03-11 Itis Holdings Plc Apparatus and methods for providing journey information
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
US8586301B2 (en) 2010-06-30 2013-11-19 Stratos Genomics, Inc. Multiplexed identification of nucleic acid sequences
CN103370617B (zh) 2010-10-01 2015-11-25 牛津纳米孔技术有限公司 生物化学分析设备和旋转阀
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
AU2012264497B2 (en) * 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
WO2013041878A1 (en) 2011-09-23 2013-03-28 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
KR102086182B1 (ko) 2011-12-29 2020-03-06 옥스포드 나노포어 테크놀로지즈 리미티드 효소 방법
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
EP3415901A1 (en) * 2012-01-20 2018-12-19 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
EP2814959B1 (en) 2012-02-17 2018-01-17 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
CN112646019B (zh) * 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
WO2013181170A1 (en) 2012-05-31 2013-12-05 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
EP2864502B1 (en) * 2012-06-20 2019-10-23 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
US9823235B2 (en) 2012-10-26 2017-11-21 Oxford Nanopre Technologies Ltd. Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
US9670526B2 (en) * 2012-11-09 2017-06-06 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CN105102627B (zh) 2013-03-15 2018-10-19 纽约哥伦比亚大学理事会 用于检测样品中多种预定化合物的方法
CN203450281U (zh) 2013-08-01 2014-02-26 大行科技(深圳)有限公司 一种折叠自行车走线结构
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015127387A1 (en) 2014-02-21 2015-08-27 Northeastern University Fluorescence-based analysis of biopolymers using nanopores
US20170096703A1 (en) 2014-03-21 2017-04-06 Oxford Nanopore Technologies Ltd. Analysis of a polymer from multi-dimensional measurements
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
US10400014B2 (en) * 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN115851894B (zh) 2014-10-16 2025-06-27 牛津楠路珀尔科技股份有限公司 聚合物的分析
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
AU2016220404B2 (en) * 2015-02-17 2021-05-27 Mgi Tech Co., Ltd. DNA sequencing using controlled strand displacement
KR102782598B1 (ko) 2015-06-25 2025-03-14 로스웰 엠이 아이엔씨. 바이오분자 센서들 및 방법들
WO2017125565A1 (en) 2016-01-21 2017-07-27 Genia Technologies, Inc. Nanopore sequencing complexes
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2016099673A1 (en) * 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

Also Published As

Publication number Publication date
AU2017367238A8 (en) 2019-07-18
AU2017367238A1 (en) 2019-06-13
WO2018100370A1 (en) 2018-06-07
EP3548636B1 (en) 2023-08-09
US11466317B2 (en) 2022-10-11
EP3548636A1 (en) 2019-10-09
US20230084931A1 (en) 2023-03-16
CA3044351A1 (en) 2018-06-07
CN110168104A (zh) 2019-08-23
CN116334200A (zh) 2023-06-27
CN116377044A (zh) 2023-07-04
US20190352709A1 (en) 2019-11-21
EP4269617A2 (en) 2023-11-01
CN116334200B (zh) 2024-10-11
JP7055805B2 (ja) 2022-04-18
GB201620450D0 (en) 2017-01-18
EP4269617A3 (en) 2024-01-10
CN116377044B (zh) 2025-05-06
JP2020500528A (ja) 2020-01-16

Similar Documents

Publication Publication Date Title
AU2017367238B2 (en) Methods and systems for characterizing analytes using nanopores
EP3802877B1 (en) Method
US20230374583A1 (en) Method of target molecule characterisation using a molecular pore
US11236385B2 (en) Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
EP3464624B1 (en) Method of nanopore sequencing of concatenated nucleic acids
US20250207188A1 (en) Method
CN107002151B (zh) 向跨膜孔输送分析物的方法
WO2018060740A1 (en) Method for nucleic acid detection by guiding through a nanopore

Legal Events

Date Code Title Description
TH Corrigenda

Free format text: IN VOL 33 , NO 22 , PAGE(S) 3247 UNDER THE HEADING PCT APPLICATIONS THAT HAVE ENTERED THE NATIONAL PHASE - NAME INDEX UNDER THE NAME OXFORD NANOPORE TECHNOLOGIES LIMITED, APPLICATION NO. 2017367238, UNDER INID (72) CORRECT THE CO-INVENTOR TO KNOTT, JESSICA MARY MAY

FGA Letters patent sealed or granted (standard patent)