CA3044351A1 - Methods and systems for characterizing analytes using nanopores - Google Patents

Methods and systems for characterizing analytes using nanopores Download PDF

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Publication number
CA3044351A1
CA3044351A1 CA3044351A CA3044351A CA3044351A1 CA 3044351 A1 CA3044351 A1 CA 3044351A1 CA 3044351 A CA3044351 A CA 3044351A CA 3044351 A CA3044351 A CA 3044351A CA 3044351 A1 CA3044351 A1 CA 3044351A1
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Prior art keywords
polynucleotide
nanopore
strand
adaptor
complement
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CA3044351A
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English (en)
French (fr)
Inventor
James Clarke
James White
Richard Muscat
Jessica Mary May KNOT
Ramiz Iqbal NATHANI
Andrew John Heron
Mark John BRUCE
Lakmal Nishantha JAYASINGHE
Domenico CAPROTTI
David Jackson STODDART
Rebecca Victoria BOWEN
Christopher James WRIGHT
Paul Richard Moody
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3044351A 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores Pending CA3044351A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1620450.5 2016-12-01
GBGB1620450.5A GB201620450D0 (en) 2016-12-01 2016-12-01 Method
US201762471338P 2017-03-14 2017-03-14
US62/471,338 2017-03-14
PCT/GB2017/053603 WO2018100370A1 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores

Publications (1)

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CA3044351A1 true CA3044351A1 (en) 2018-06-07

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CA3044351A Pending CA3044351A1 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores

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US (2) US11466317B2 (enExample)
EP (2) EP3548636B1 (enExample)
JP (1) JP7055805B2 (enExample)
CN (3) CN116334200B (enExample)
AU (1) AU2017367238B2 (enExample)
CA (1) CA3044351A1 (enExample)
GB (1) GB201620450D0 (enExample)
WO (1) WO2018100370A1 (enExample)

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EP3548636A1 (en) 2019-10-09
US20230084931A1 (en) 2023-03-16
US12509721B2 (en) 2025-12-30
EP4269617A2 (en) 2023-11-01
CN116377044B (zh) 2025-05-06
US11466317B2 (en) 2022-10-11
AU2017367238A8 (en) 2019-07-18
CN116334200B (zh) 2024-10-11
JP7055805B2 (ja) 2022-04-18
EP4269617A3 (en) 2024-01-10
EP3548636B1 (en) 2023-08-09
AU2017367238B2 (en) 2021-10-21
US20190352709A1 (en) 2019-11-21
AU2017367238A1 (en) 2019-06-13
CN110168104A (zh) 2019-08-23
CN116334200A (zh) 2023-06-27
CN116377044A (zh) 2023-07-04
JP2020500528A (ja) 2020-01-16
GB201620450D0 (en) 2017-01-18
WO2018100370A1 (en) 2018-06-07

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