JP2017537645A5 - - Google Patents

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JP2017537645A5
JP2017537645A5 JP2017532096A JP2017532096A JP2017537645A5 JP 2017537645 A5 JP2017537645 A5 JP 2017537645A5 JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017537645 A5 JP2017537645 A5 JP 2017537645A5
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JP
Japan
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barcode
epitope
unique binding
cells
cell origin
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JP2017532096A
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JP2017537645A (ja
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Priority claimed from PCT/US2015/067147 external-priority patent/WO2016100976A2/en
Publication of JP2017537645A publication Critical patent/JP2017537645A/ja
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JP2017532096A 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法 Pending JP2017537645A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462094924P 2014-12-19 2014-12-19
US201462094919P 2014-12-19 2014-12-19
US201462094917P 2014-12-19 2014-12-19
US62/094,919 2014-12-19
US62/094,917 2014-12-19
US62/094,924 2014-12-19
PCT/US2015/067147 WO2016100976A2 (en) 2014-12-19 2015-12-21 Methods for identifying multiple epitopes in selected sub-populations of cells

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JP2020186442A Division JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

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JP2017537645A JP2017537645A (ja) 2017-12-21
JP2017537645A5 true JP2017537645A5 (enExample) 2019-01-17

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JP2017532096A Pending JP2017537645A (ja) 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法
JP2020186442A Active JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

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US (3) US20180320241A1 (enExample)
EP (1) EP3234602B1 (enExample)
JP (2) JP2017537645A (enExample)
CN (1) CN107110849B (enExample)
WO (1) WO2016100976A2 (enExample)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11560585B2 (en) 2011-01-31 2023-01-24 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
CN103703143B (zh) 2011-01-31 2016-12-14 爱普瑞斯生物公司 鉴定细胞中的多个表位的方法
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CN107406888A (zh) 2015-03-30 2017-11-28 赛卢拉研究公司 用于组合条形编码的方法和组合物
EP3286326B1 (en) 2015-04-23 2025-01-22 Becton, Dickinson and Company Method for whole transcriptome amplification
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3488239B1 (en) * 2016-07-22 2026-03-25 Verily Life Sciences LLC Quantitative massively parallel proteomics
IL265478B2 (en) 2016-09-26 2024-10-01 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622222D0 (en) * 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
EP3574112B1 (en) * 2017-01-27 2023-01-11 Roche Diagnostics GmbH Barcoded dna for long range sequencing
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
EP3447152A1 (en) * 2017-08-25 2019-02-27 Eawag, Swiss Federal Institute of Aquatic Science and Technology Accurate and massively parallel quantification of nucleic acid
DK3684949T3 (en) * 2017-09-22 2024-07-08 Univ Washington In situ combinatorial labeling of cellular molecules
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
WO2019099908A1 (en) 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN114807306A (zh) * 2017-12-08 2022-07-29 10X基因组学有限公司 用于标记细胞的方法和组合物
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
US11039856B2 (en) 2018-05-10 2021-06-22 Verily Life Sciences Llc Surgical laparoscopic ports for implanting medical systems
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
EP3861134B1 (en) 2018-10-01 2024-09-04 Becton, Dickinson and Company Determining 5' transcript sequences
EP3877520B1 (en) 2018-11-08 2025-03-19 Becton, Dickson And Company Whole transcriptome analysis of single cells using random priming
AU2019384154B2 (en) * 2018-11-21 2025-11-20 Agilent Technologies, Inc. Methods for targeted nucleic acid library formation
JP7332694B2 (ja) * 2018-12-04 2023-08-23 エフ． ホフマン－ラ ロシュ アーゲー 細胞標的の空間指向性量子バーコード化
EP3894552B1 (en) 2018-12-13 2026-01-28 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
SG10201904897UA (en) * 2019-05-30 2020-12-30 Nat Univ Singapore Method, structures and system for nucleic acid sequence topology assembly for multiplexed profiling of proteins.
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
WO2021034791A1 (en) * 2019-08-19 2021-02-25 Inscripta, Inc. Optimizing high-throughput sequencing capacity
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ， インク． 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
CN115279919A (zh) 2020-01-13 2022-11-01 贝克顿迪金森公司 使用含有du的寡核苷酸的细胞捕获
WO2021146393A1 (en) * 2020-01-14 2021-07-22 William Marsh Rice University High throughput genetic barcoding and analysis methods
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
EP4162069A1 (en) * 2020-06-08 2023-04-12 F. Hoffmann-La Roche AG Method of single-cell analysis of multiple samples
US20230340578A1 (en) * 2020-07-08 2023-10-26 Roche Sequencing Solutions, Inc. Spatial analysis of multiple targets in tissue samples
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
US20240336959A1 (en) * 2020-12-22 2024-10-10 Pixelgen Technologies Ab Method for making a physical map of a population of barcoded particles
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US12704508B2 (en) 2021-06-02 2026-08-11 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (NULISA)
EP4399327B1 (en) * 2021-09-08 2025-10-29 Becton, Dickinson and Company Non-sequencing pcr-based method for detection of antibody-conjugated oligonucleotides
WO2023235179A1 (en) * 2022-05-30 2023-12-07 The Regents Of The University Of California Methods and compositions for generating spatially resolved genomic profiles from tissues

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
SE9400088D0 (sv) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6799120B2 (en) * 2000-02-03 2004-09-28 Nanoscale Combinatorial Synthesis, Inc. Nonredundant split/pool synthesis of combinatorial libraries
SE516272C2 (sv) * 2000-02-18 2001-12-10 Ulf Landegren Metoder och kit för analytdetektion mha proximitets-probning
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
WO2005123963A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
EP2185720A4 (en) * 2007-08-10 2010-09-01 Bio Inc Primity BIOLOGICAL CODING OF A LARGE NUMBER OF CELLS
EP2279291B1 (en) * 2008-04-09 2019-07-03 Cornell University Coferons and methods of making and using them
GB0816976D0 (en) * 2008-09-16 2008-10-22 Natural Environment Res Council Protein
US8481698B2 (en) * 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
CN103703143B (zh) * 2011-01-31 2016-12-14 爱普瑞斯生物公司 鉴定细胞中的多个表位的方法
JP6153874B2 (ja) * 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
EP4450644A3 (en) * 2012-08-08 2025-01-08 F. Hoffmann-La Roche AG Increasing dynamic range for identifying multiple epitopes in cells
IL308158A (en) * 2012-12-17 2023-12-01 Harvard College Rna-guided human genome engineering

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