JP2015508893A5 - - Google Patents

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JP2015508893A5
JP2015508893A5 JP2014557118A JP2014557118A JP2015508893A5 JP 2015508893 A5 JP2015508893 A5 JP 2015508893A5 JP 2014557118 A JP2014557118 A JP 2014557118A JP 2014557118 A JP2014557118 A JP 2014557118A JP 2015508893 A5 JP2015508893 A5 JP 2015508893A5
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analyte
probe
panel
aptamer
members
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JP2014557118A
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JP2015508893A (ja
JP6275052B2 (ja
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Priority claimed from PCT/GB2013/050348 external-priority patent/WO2013121201A1/en
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JP2014557118A 2012-02-15 2013-02-14 アプタマー法 Active JP6275052B2 (ja)

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Application Number Priority Date Filing Date Title
US201261599240P 2012-02-15 2012-02-15
US61/599,240 2012-02-15
PCT/GB2013/050348 WO2013121201A1 (en) 2012-02-15 2013-02-14 Aptamer method

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JP2015508893A JP2015508893A (ja) 2015-03-23
JP2015508893A5 true JP2015508893A5 (enExample) 2016-03-31
JP6275052B2 JP6275052B2 (ja) 2018-02-07

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US (3) US10739341B2 (enExample)
EP (1) EP2815235B1 (enExample)
JP (1) JP6275052B2 (enExample)
CN (1) CN104220874B (enExample)
AU (1) AU2013220156B2 (enExample)
CA (1) CA2864035C (enExample)
IN (1) IN2014DN06796A (enExample)
WO (1) WO2013121201A1 (enExample)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2012009578A2 (en) 2010-07-14 2012-01-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
US10739341B2 (en) 2012-02-15 2020-08-11 Oxford Nanopore Technologies Limited Aptamer method
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
JP2016517275A (ja) 2013-03-15 2016-06-16 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ 多重核酸検出のためのコード化ナノポアセンサー
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method
GB201406151D0 (en) * 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
WO2016034591A2 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant pores
JP6485855B2 (ja) * 2014-10-03 2019-03-20 国立研究開発法人理化学研究所 アプタマーを用いた被検物の電気化学検出法
KR102457147B1 (ko) 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
JP7237586B2 (ja) 2015-12-08 2023-03-13 カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント 修飾ナノポア、それを含む組成物およびその使用
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
CA3020203A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
CN105974125A (zh) * 2016-05-22 2016-09-28 杜护侠 一种心脑血管疾病特异性检测试剂盒
US10191040B2 (en) * 2016-09-30 2019-01-29 Chin-Yih Hong Method of multiplex immunoassays utilizing differential affinity and methods for synthesizing aptamer-based reagents for multiplex immunoassays
GB201617886D0 (en) * 2016-10-21 2016-12-07 Oxford Nanopore Technologies Limited Method
WO2018093976A1 (en) * 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag
GB201705764D0 (en) 2017-04-10 2017-05-24 Imp Innovations Ltd Analyte detection method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN110914290A (zh) 2017-06-30 2020-03-24 弗拉芒区生物技术研究所 新颖蛋白孔
GB201714478D0 (en) 2017-09-08 2017-10-25 Univ Bristol Sensor
CN112423878B (zh) * 2018-02-12 2024-06-18 牛津纳米孔科技公开有限公司 纳米孔组件及其用途
CN111919118B (zh) * 2018-04-13 2025-09-05 豪夫迈·罗氏有限公司 用于检测和分析分析物的方法和组合物
US20200326325A1 (en) 2019-04-12 2020-10-15 Lisa Diamond Nanosensor chip with compound nanopores
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
JPH08500722A (ja) 1992-01-29 1996-01-30 日立化成工業株式会社 ポリヌクレオチド固定化担体
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
US6246231B1 (en) * 1999-07-29 2001-06-12 Ascension Technology Corporation Magnetic field permeable barrier for magnetic position measurement system
CA2384100C (en) 1999-09-07 2011-03-29 The Regents Of The University Of California Methods of determining the presence of double stranded nucleic acids in a sample
EP1097993A3 (en) 1999-11-05 2004-01-07 National Institute of Advanced Industrial Science and Technology Functional ribozyme chimeric molecules capable of sliding
US20030008295A1 (en) * 2000-03-06 2003-01-09 Nassim Usman Nucleic acid sensor molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US6706203B2 (en) * 2001-10-30 2004-03-16 Agilent Technologies, Inc. Adjustable nanopore, nanotome, and nanotweezer
AU2003299507A1 (en) 2002-05-17 2004-06-07 Board Of Regents, The University Of Texas System Hop - a novel cardiac-restricted transcriptional factor potentially useful for cardiac regeneration and specification
EP1539979B1 (en) 2002-09-20 2008-11-19 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US7347921B2 (en) 2003-07-17 2008-03-25 Agilent Technologies, Inc. Apparatus and method for threading a biopolymer through a nanopore
US20060030535A1 (en) * 2004-03-05 2006-02-09 Healy Judith M Controlled modulation of the pharmacokinetics and biodistribution of aptamer therapeutics
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US20090264299A1 (en) * 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
US9233846B2 (en) * 2005-10-14 2016-01-12 The Regents Of The University Of California Formation and encapsulation of molecular bilayer and monolayer membranes
WO2007120265A2 (en) * 2005-11-14 2007-10-25 Applera Corporation Coded molecules for detecting target analytes
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP1994179A2 (en) * 2006-02-18 2008-11-26 Michael Strathmann Massively multiplexed sequencing
WO2007117832A2 (en) * 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
US20110118187A1 (en) * 2006-10-19 2011-05-19 Duke University Reversible platelet inhibition
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
US8500982B2 (en) * 2007-04-04 2013-08-06 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
EP2212434A1 (en) 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase ligation sequencing
EP2209893B1 (en) * 2007-10-12 2013-11-20 Pronota NV Use of aptamers in proteomics
WO2009053691A1 (en) * 2007-10-22 2009-04-30 Pronota Nv Method of selecting aptamers
GB0724736D0 (en) * 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CN102066562A (zh) * 2008-03-12 2011-05-18 雪城大学 结构限定的适配子的直接选择
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
WO2010047797A2 (en) * 2008-10-21 2010-04-29 Research Foundation Of The City University Of New York Single quantum-dot-based aptameric sensors
US20110008311A2 (en) 2008-11-03 2011-01-13 The Board Of Trustees Of The University Of Illinois Animal Model and Use of 17Beta-Hydroxysteroid Dehydrogenase Type 7 in the Diagnosis of Anencephaly
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
BRPI1007215A2 (pt) 2009-01-30 2017-08-29 Oxford Nanopore Tech Ltd Método de acoplamento covalente de duas ou mais porções, primeira e segunda porções, primeiro porção acoplada a uma segunda porção, par de primeiro e segundo ligantes, e, uso de um par de ligantes.
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010117470A2 (en) 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
CA2781581C (en) 2009-12-01 2018-03-06 Oxford Nanopore Technologies Limited Biochemical analysis instrument
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
EP2537026B1 (en) 2010-02-19 2018-06-27 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
US8557529B2 (en) * 2010-04-09 2013-10-15 International Business Machines Corporation Nanopore capture system
WO2012009578A2 (en) * 2010-07-14 2012-01-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
CN103392008B (zh) 2010-09-07 2017-10-20 加利福尼亚大学董事会 通过持续性酶以一个核苷酸的精度控制dna在纳米孔中的移动
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
EP3848706B1 (en) 2011-05-27 2023-07-19 Oxford Nanopore Technologies PLC Coupling method
BR112014001699A2 (pt) 2011-07-25 2017-06-13 Oxford Nanopore Tech Ltd método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho
JP2014521956A (ja) 2011-07-27 2014-08-28 ザ ボード オブ トラスティーズ オブ ザ ユニヴァーシティー オブ イリノイ 生体分子を特徴付けるためのナノ細孔センサー
SG11201401628WA (en) 2011-10-19 2014-05-29 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
CN102430153A (zh) 2011-12-24 2012-05-02 中国人民解放军第三军医大学第二附属医院 一种基于交联技术的仿生骨界面、构建方法及其应用
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
EP2798084B1 (en) 2011-12-29 2017-04-19 Oxford Nanopore Technologies Limited Enzyme method
US10739341B2 (en) 2012-02-15 2020-08-11 Oxford Nanopore Technologies Limited Aptamer method
BR102012007246A2 (pt) 2012-03-30 2016-02-10 Fundação Antonio Prudente métodos para prognóstico e classificação de resultados de um evento
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
WO2014013262A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
KR102457147B1 (ko) 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
JP6967006B2 (ja) * 2016-01-15 2021-11-17 クアンタポール, インコーポレイテッド 低減されたバックグラウンドの光学に基づくナノポア分析

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