AU2013220156B2 - Aptamer method - Google Patents

Aptamer method Download PDF

Info

Publication number
AU2013220156B2
AU2013220156B2 AU2013220156A AU2013220156A AU2013220156B2 AU 2013220156 B2 AU2013220156 B2 AU 2013220156B2 AU 2013220156 A AU2013220156 A AU 2013220156A AU 2013220156 A AU2013220156 A AU 2013220156A AU 2013220156 B2 AU2013220156 B2 AU 2013220156B2
Authority
AU
Australia
Prior art keywords
lys
asn
pore
ser
thr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2013220156A
Other languages
English (en)
Other versions
AU2013220156A1 (en
Inventor
Clive Gavin Brown
James Anthony Clarke
Daniel George FORDHAM
Roger Charles GILL
Stuart Reid
Daniel John Turner
James White
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Publication of AU2013220156A1 publication Critical patent/AU2013220156A1/en
Application granted granted Critical
Publication of AU2013220156B2 publication Critical patent/AU2013220156B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/36—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Actinomyces; from Streptomyces (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/49—Platelet-derived growth factor [PDGF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96463—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
AU2013220156A 2012-02-15 2013-02-14 Aptamer method Active AU2013220156B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261599240P 2012-02-15 2012-02-15
US61/599,240 2012-02-15
PCT/GB2013/050348 WO2013121201A1 (en) 2012-02-15 2013-02-14 Aptamer method

Publications (2)

Publication Number Publication Date
AU2013220156A1 AU2013220156A1 (en) 2014-09-18
AU2013220156B2 true AU2013220156B2 (en) 2018-08-09

Family

ID=47749885

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013220156A Active AU2013220156B2 (en) 2012-02-15 2013-02-14 Aptamer method

Country Status (8)

Country Link
US (3) US10739341B2 (enExample)
EP (1) EP2815235B1 (enExample)
JP (1) JP6275052B2 (enExample)
CN (1) CN104220874B (enExample)
AU (1) AU2013220156B2 (enExample)
CA (1) CA2864035C (enExample)
IN (1) IN2014DN06796A (enExample)
WO (1) WO2013121201A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12704516B2 (en) 2020-05-20 2026-08-11 The Board Of Trustees Of The University Of Illinois Methods and compositions for rapid direct detection and differentiation of infectious from noninfectious virus

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP2593568B1 (en) 2010-07-14 2017-04-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
CA2864035C (en) 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
WO2014041337A1 (en) 2012-09-14 2014-03-20 Oxford Nanopore Technologies Limited Sample preparation method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
US9732379B2 (en) 2013-03-15 2017-08-15 The Curators Of The University Of Missouri Encoded nanopore sensor for multiplex nucleic acids detection
GB201313477D0 (en) * 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method
GB201406151D0 (en) * 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
JP6485855B2 (ja) * 2014-10-03 2019-03-20 国立研究開発法人理化学研究所 アプタマーを用いた被検物の電気化学検出法
WO2016059436A1 (en) 2014-10-17 2016-04-21 Oxford Nanopore Technologies Limited Method for nanopore rna characterisation
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
CN107735686B (zh) 2015-04-14 2021-06-11 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔，包含其的组合物及其应用
KR102222191B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
CN118272512A (zh) 2016-04-06 2024-07-02 牛津纳米孔科技公开有限公司 突变体孔
CN105974125A (zh) * 2016-05-22 2016-09-28 杜护侠 一种心脑血管疾病特异性检测试剂盒
US10191040B2 (en) 2016-09-30 2019-01-29 Chin-Yih Hong Method of multiplex immunoassays utilizing differential affinity and methods for synthesizing aptamer-based reagents for multiplex immunoassays
GB201617886D0 (en) 2016-10-21 2016-12-07 Oxford Nanopore Technologies Limited Method
WO2018093976A1 (en) * 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag
GB201705764D0 (en) 2017-04-10 2017-05-24 Imp Innovations Ltd Analyte detection method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
EP3645552B1 (en) 2017-06-30 2023-06-28 Vib Vzw Novel protein pores
GB201714478D0 (en) 2017-09-08 2017-10-25 Univ Bristol Sensor
CN118949941A (zh) * 2018-02-12 2024-11-15 牛津纳米孔科技公开有限公司 纳米孔组件及其用途
WO2019197590A1 (en) * 2018-04-13 2019-10-17 F. Hoffmann-La Roche Ag Methods and compositions for detection and analysis of analytes
US20200326325A1 (en) 2019-04-12 2020-10-15 Lisa Diamond Nanosensor chip with compound nanopores
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
MX9300492A (es) 1992-01-29 1994-07-29 Hitachi Chemical Co Ltd Soporte inmovilizado de nucleotido y metodo para su produccion.
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) * 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
US6246231B1 (en) * 1999-07-29 2001-06-12 Ascension Technology Corporation Magnetic field permeable barrier for magnetic position measurement system
EP1212462B1 (en) 1999-09-07 2006-01-25 The Regents of the University of California Methods of determining the presence of double stranded nucleic acids in a sample
EP1097993A3 (en) 1999-11-05 2004-01-07 National Institute of Advanced Industrial Science and Technology Functional ribozyme chimeric molecules capable of sliding
US20030008295A1 (en) * 2000-03-06 2003-01-09 Nassim Usman Nucleic acid sensor molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US6706203B2 (en) * 2001-10-30 2004-03-16 Agilent Technologies, Inc. Adjustable nanopore, nanotome, and nanotweezer
WO2004041843A2 (en) 2002-05-17 2004-05-21 Board Of Regents, The University Of Texas System Hop - a novel cardiac-restricted transcriptional factor potentially useful for cardiac regeneration and specification
AU2003272438B2 (en) 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US7347921B2 (en) 2003-07-17 2008-03-25 Agilent Technologies, Inc. Apparatus and method for threading a biopolymer through a nanopore
EP1732571A4 (en) * 2004-03-05 2009-09-09 Archemix Corp CONTROLLED MODULATION OF PHARMACOKINETICS AND BIODISTRIBUTION OF APTAMER THERAPEUTICS
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US9233846B2 (en) * 2005-10-14 2016-01-12 The Regents Of The University Of California Formation and encapsulation of molecular bilayer and monolayer membranes
US20070190543A1 (en) * 2005-11-14 2007-08-16 Applera Corporation Coded Molecules for Detecting Target Analytes
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
WO2007098427A2 (en) * 2006-02-18 2007-08-30 Michael Strathmann Massively multiplexed sequencing
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US20100291548A1 (en) * 2006-03-12 2010-11-18 Applera Corporation Methods of Detecting Target Nucleic Acids
US20110118187A1 (en) * 2006-10-19 2011-05-19 Duke University Reversible platelet inhibition
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
WO2008124107A1 (en) * 2007-04-04 2008-10-16 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
US20090191553A1 (en) 2007-10-01 2009-07-30 Applied Biosystems Inc. Chase Ligation Sequencing
ES2448426T3 (es) * 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
EP2209914B2 (en) * 2007-10-22 2017-07-26 Caris Life Sciences Switzerland Holdings GmbH Method of selecting aptamers
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CN102066562A (zh) * 2008-03-12 2011-05-18 雪城大学 结构限定的适配子的直接选择
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CA2730068A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
US8642260B2 (en) * 2008-10-21 2014-02-04 The Research Foundation Of The City University Of New York Single quantum-dot based aptameric nanosensors
US20110008311A2 (en) 2008-11-03 2011-01-13 The Board Of Trustees Of The University Of Illinois Animal Model and Use of 17Beta-Hydroxysteroid Dehydrogenase Type 7 in the Diagnosis of Anencephaly
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010117470A2 (en) 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CA2758617C (en) 2009-04-20 2017-10-03 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
EP2537026B1 (en) 2010-02-19 2018-06-27 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
US8557529B2 (en) * 2010-04-09 2013-10-15 International Business Machines Corporation Nanopore capture system
EP2593568B1 (en) 2010-07-14 2017-04-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
EP2614156B1 (en) 2010-09-07 2018-08-01 The Regents of The University of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
BR112013020411B1 (pt) 2011-02-11 2021-09-08 Oxford Nanopore Technologies Limited Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
KR20140050067A (ko) 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
WO2013016486A1 (en) 2011-07-27 2013-01-31 The Board Of Trustees Of The University Of Illinois Nanopore sensors for biomolecular characterization
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
CN102430153A (zh) 2011-12-24 2012-05-02 中国人民解放军第三军医大学第二附属医院 一种基于交联技术的仿生骨界面、构建方法及其应用
CA2861808C (en) 2011-12-29 2021-02-23 Oxford Nanopore Technologies Limited Enzyme method
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
CA2864035C (en) 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
BR102012007246A2 (pt) 2012-03-30 2016-02-10 Fundação Antonio Prudente métodos para prognóstico e classificação de resultados de um evento
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
CA2879355C (en) 2012-07-19 2021-09-21 Oxford Nanopore Technologies Limited Helicase construct and its use in characterising polynucleotides
WO2014041337A1 (en) 2012-09-14 2014-03-20 Oxford Nanopore Technologies Limited Sample preparation method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
WO2016059436A1 (en) 2014-10-17 2016-04-21 Oxford Nanopore Technologies Limited Method for nanopore rna characterisation
CN108463560B (zh) * 2016-01-15 2022-09-20 昆塔波尔公司 具有降低的背景的基于光学的纳米孔分析

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DVIR ROTEM ET AL, "Protein Detection by Nanopores Equipped with Aptamers", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, (2012-02-08), vol. 134, no. 5, doi:10.1021/ja2105653, ISSN 0002-7863, pages 2781 - 2787 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12704516B2 (en) 2020-05-20 2026-08-11 The Board Of Trustees Of The University Of Illinois Methods and compositions for rapid direct detection and differentiation of infectious from noninfectious virus

Also Published As

Publication number Publication date
EP2815235A1 (en) 2014-12-24
JP2015508893A (ja) 2015-03-23
IN2014DN06796A (enExample) 2015-05-22
US20210018500A1 (en) 2021-01-21
JP6275052B2 (ja) 2018-02-07
CA2864035C (en) 2021-05-18
US10739341B2 (en) 2020-08-11
CN104220874A (zh) 2014-12-17
CA2864035A1 (en) 2013-08-22
AU2013220156A1 (en) 2014-09-18
US11685922B2 (en) 2023-06-27
US20230392157A1 (en) 2023-12-07
WO2013121201A1 (en) 2013-08-22
US20150177237A1 (en) 2015-06-25
EP2815235B1 (en) 2020-04-15
CN104220874B (zh) 2017-05-24

Similar Documents

Publication Publication Date Title
US20230392157A1 (en) Aptamer method
US20230212665A1 (en) Enzyme stalling method
EP2917366B1 (en) Quadruplex method
KR102086182B1 (ko) 효소 방법
EP2798083B1 (en) Method for characterising a polynucelotide by using a xpd helicase
AU2012324639B2 (en) Method of characterizing a target polynucleotide using a pore and a Hel308 helicase
US11466317B2 (en) Methods and systems for characterizing analytes using nanopores
EP3207157B1 (en) Methods for delivering an analyte to transmembrane pores
EP3192804B1 (en) Mutant lysenin pores
US9551023B2 (en) Sample preparation method
KR20140050067A (ko) 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
CN105765387A (zh) 一种方法
US20250283153A1 (en) Multiplex methods of detecting molecules using nanopores

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)