CA2864035C - A method for determining the presence of an analyte using an aptamer - Google Patents

A method for determining the presence of an analyte using an aptamer Download PDF

Info

Publication number
CA2864035C
CA2864035C CA2864035A CA2864035A CA2864035C CA 2864035 C CA2864035 C CA 2864035C CA 2864035 A CA2864035 A CA 2864035A CA 2864035 A CA2864035 A CA 2864035A CA 2864035 C CA2864035 C CA 2864035C
Authority
CA
Canada
Prior art keywords
determining
analyte
aptamer
absence
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2864035A
Other languages
English (en)
French (fr)
Other versions
CA2864035A1 (en
Inventor
Daniel John Turner
Daniel George FORDHAM
Roger Charles GILL
Clive Gavin Brown
Stuart Reid
James Anthony Clarke
James White
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Publication of CA2864035A1 publication Critical patent/CA2864035A1/en
Application granted granted Critical
Publication of CA2864035C publication Critical patent/CA2864035C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/36—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Actinomyces; from Streptomyces (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/49—Platelet-derived growth factor [PDGF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96463—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CA2864035A 2012-02-15 2013-02-14 A method for determining the presence of an analyte using an aptamer Active CA2864035C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261599240P 2012-02-15 2012-02-15
US61/599,240 2012-02-15
PCT/GB2013/050348 WO2013121201A1 (en) 2012-02-15 2013-02-14 Aptamer method

Publications (2)

Publication Number Publication Date
CA2864035A1 CA2864035A1 (en) 2013-08-22
CA2864035C true CA2864035C (en) 2021-05-18

Family

ID=47749885

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2864035A Active CA2864035C (en) 2012-02-15 2013-02-14 A method for determining the presence of an analyte using an aptamer

Country Status (8)

Country Link
US (3) US10739341B2 (enExample)
EP (1) EP2815235B1 (enExample)
JP (1) JP6275052B2 (enExample)
CN (1) CN104220874B (enExample)
AU (1) AU2013220156B2 (enExample)
CA (1) CA2864035C (enExample)
IN (1) IN2014DN06796A (enExample)
WO (1) WO2013121201A1 (enExample)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
JP6016792B2 (ja) 2010-07-14 2016-10-26 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ ナノポア促進型の核酸の単分子検出
AU2013220156B2 (en) 2012-02-15 2018-08-09 Oxford Nanopore Technologies Limited Aptamer method
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
EP2895618B1 (en) 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
US9995728B2 (en) 2012-11-06 2018-06-12 Oxford Nanopore Technologies Ltd. Quadruplex method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
WO2014144217A1 (en) 2013-03-15 2014-09-18 The Curators Of The University Of Missouri Encoded nanopore sensor for multiplex nucleic acids detection
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
GB201316849D0 (en) 2013-09-23 2013-11-06 Isis Innovation Method
GB201406151D0 (en) * 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
JP6485855B2 (ja) * 2014-10-03 2019-03-20 国立研究開発法人理化学研究所 アプタマーを用いた被検物の電気化学検出法
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
EP3283887B1 (en) 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
KR102222192B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
JP7364333B2 (ja) 2016-04-06 2023-10-18 オックスフォード ナノポール テクノロジーズ ピーエルシー 変異体ポア
CN105974125A (zh) * 2016-05-22 2016-09-28 杜护侠 一种心脑血管疾病特异性检测试剂盒
US10191040B2 (en) * 2016-09-30 2019-01-29 Chin-Yih Hong Method of multiplex immunoassays utilizing differential affinity and methods for synthesizing aptamer-based reagents for multiplex immunoassays
GB201617886D0 (en) 2016-10-21 2016-12-07 Oxford Nanopore Technologies Limited Method
WO2018093976A1 (en) * 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag
GB201705764D0 (en) 2017-04-10 2017-05-24 Imp Innovations Ltd Analyte detection method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
GB201714478D0 (en) 2017-09-08 2017-10-25 Univ Bristol Sensor
US20200399693A1 (en) * 2018-02-12 2020-12-24 Oxford Nanopore Technologies, Ltd. Nanopore assemblies and uses thereof
WO2019197590A1 (en) * 2018-04-13 2019-10-17 F. Hoffmann-La Roche Ag Methods and compositions for detection and analysis of analytes
US20200326325A1 (en) 2019-04-12 2020-10-15 Lisa Diamond Nanosensor chip with compound nanopores
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
CA2128891A1 (en) 1992-01-29 1993-08-05 Cylia Keller Polynucleotide immobilized support
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) * 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
US6246231B1 (en) * 1999-07-29 2001-06-12 Ascension Technology Corporation Magnetic field permeable barrier for magnetic position measurement system
DE60025739T2 (de) 1999-09-07 2006-08-31 The Regents Of The University Of California, Oakland Verfahren um die anwesenheit von doppelsträngiger dns in einer probe nachzuweisen
EP1097993A3 (en) 1999-11-05 2004-01-07 National Institute of Advanced Industrial Science and Technology Functional ribozyme chimeric molecules capable of sliding
US20030008295A1 (en) * 2000-03-06 2003-01-09 Nassim Usman Nucleic acid sensor molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US6706203B2 (en) * 2001-10-30 2004-03-16 Agilent Technologies, Inc. Adjustable nanopore, nanotome, and nanotweezer
US20040029158A1 (en) 2002-05-17 2004-02-12 Eric Olson HOP - a novel cardiac-restricted transcriptional factor potentially useful for cardiac regeneration and specification
AU2003272438B2 (en) 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US7347921B2 (en) 2003-07-17 2008-03-25 Agilent Technologies, Inc. Apparatus and method for threading a biopolymer through a nanopore
US20060030535A1 (en) * 2004-03-05 2006-02-09 Healy Judith M Controlled modulation of the pharmacokinetics and biodistribution of aptamer therapeutics
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US20090264299A1 (en) * 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
US9233846B2 (en) * 2005-10-14 2016-01-12 The Regents Of The University Of California Formation and encapsulation of molecular bilayer and monolayer membranes
EP1954838B1 (en) * 2005-11-14 2014-02-26 Life Technologies Corporation Coded molecules for detecting target analytes
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US20070224613A1 (en) * 2006-02-18 2007-09-27 Strathmann Michael P Massively Multiplexed Sequencing
WO2007117832A2 (en) * 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
WO2008066621A2 (en) * 2006-10-19 2008-06-05 Duke University Reversible platelet inhibition
NZ579083A (en) 2007-02-20 2012-07-27 Oxford Nanopore Tech Ltd Lipid bilayer sensor system
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
WO2009046149A1 (en) 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
ES2448426T3 (es) * 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
WO2009053691A1 (en) * 2007-10-22 2009-04-30 Pronota Nv Method of selecting aptamers
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US20110263459A1 (en) * 2008-03-12 2011-10-27 Borer Philip N Direct selection of structurally defined aptamers
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9447152B2 (en) 2008-07-07 2016-09-20 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
WO2010047797A2 (en) * 2008-10-21 2010-04-29 Research Foundation Of The City University Of New York Single quantum-dot-based aptameric sensors
US20110008311A2 (en) 2008-11-03 2011-01-13 The Board Of Trustees Of The University Of Illinois Animal Model and Use of 17Beta-Hydroxysteroid Dehydrogenase Type 7 in the Diagnosis of Anencephaly
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
CA2750874A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
WO2011103424A2 (en) 2010-02-19 2011-08-25 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
US8557529B2 (en) * 2010-04-09 2013-10-15 International Business Machines Corporation Nanopore capture system
JP6016792B2 (ja) * 2010-07-14 2016-10-26 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ ナノポア促進型の核酸の単分子検出
WO2012033524A2 (en) 2010-09-07 2012-03-15 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
GB2500360B (en) 2010-12-22 2019-10-23 Genia Tech Inc Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
JP2014521956A (ja) 2011-07-27 2014-08-28 ザ ボード オブ トラスティーズ オブ ザ ユニヴァーシティー オブ イリノイ 生体分子を特徴付けるためのナノ細孔センサー
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
CN102430153A (zh) 2011-12-24 2012-05-02 中国人民解放军第三军医大学第二附属医院 一种基于交联技术的仿生骨界面、构建方法及其应用
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
KR102086182B1 (ko) 2011-12-29 2020-03-06 옥스포드 나노포어 테크놀로지즈 리미티드 효소 방법
AU2013220156B2 (en) 2012-02-15 2018-08-09 Oxford Nanopore Technologies Limited Aptamer method
BR102012007246A2 (pt) 2012-03-30 2016-02-10 Fundação Antonio Prudente métodos para prognóstico e classificação de resultados de um evento
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
EP2895618B1 (en) 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
US9995728B2 (en) 2012-11-06 2018-06-12 Oxford Nanopore Technologies Ltd. Quadruplex method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
WO2017123647A1 (en) * 2016-01-15 2017-07-20 Quantapore, Inc. Optically-based nanopore analysis with reduced background

Also Published As

Publication number Publication date
JP6275052B2 (ja) 2018-02-07
CA2864035A1 (en) 2013-08-22
US10739341B2 (en) 2020-08-11
AU2013220156A1 (en) 2014-09-18
US20150177237A1 (en) 2015-06-25
US20230392157A1 (en) 2023-12-07
CN104220874A (zh) 2014-12-17
EP2815235A1 (en) 2014-12-24
AU2013220156B2 (en) 2018-08-09
WO2013121201A1 (en) 2013-08-22
US20210018500A1 (en) 2021-01-21
EP2815235B1 (en) 2020-04-15
JP2015508893A (ja) 2015-03-23
IN2014DN06796A (enExample) 2015-05-22
CN104220874B (zh) 2017-05-24
US11685922B2 (en) 2023-06-27

Similar Documents

Publication Publication Date Title
CA2864035C (en) A method for determining the presence of an analyte using an aptamer
PH12020551028A1 (en) Methods, kits and apparatus for expanding a population of cells
AU2017367238A8 (en) Methods and systems for characterizing analytes using nanopores
EP2668501A4 (en) Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof
BR112014018831A2 (pt) método para otimizar sensibilidade e/ou seletividade de um sensor para um ou mais analitos, método para formar um sensor para um ou mais analitos, kit para a detecção de um ou mais analitos, método de identificação de um aptâmero, composição de matéria e aptâmeros.
BR112015001592A8 (pt) análise de imagem e medidas de amostras biológicas
PH12014502581B1 (en) Aptamer-based multiplexed assays
EP2673631A1 (en) Methods and systems for determining the presence or amount of testosterone in a sample
EP4249605A3 (en) Methods for analyte detection
CR20140212A (es) Anticuerpos anti-htra1 y métodos de uso
EP3004385A4 (en) Nanopore-based nucleic acid analysis with mixed fret detection
MX353954B (es) Aptameros y metodos de diagnostico para detectar el receptor del factor de crecimiento epidermal (egf).
EP3311140B8 (en) Determining extracellular analyte concentration with nanoplasmonic sensors
WO2013166338A3 (en) Cell capture system and use thereof
EP4527921A3 (en) Renal cell populations and uses thereof
EP4563709A3 (en) Methods and materials for assessing allelic imbalance
GB201011459D0 (en) Optical element, assembly and method for determining analyte concentration
WO2012170206A3 (en) Color-producing diagnostic systems, reagents and methods
EP2929068A4 (en) ELECTROCHEMICAL DISTRIBUTION SYSTEMS AND METHOD
WO2012164525A3 (en) Aging biomarkers
GB2496557A (en) Products and methods for identifying rock samples
MY165647A (en) Psa assay and reagent therefor
MX2014014381A (es) Anticuerpos de anti-teofilina y metodos de uso.
MX2015004419A (es) Deteccion de analito y determinacion de concentracion de analito mediante uso de perlas magnetizables.
WO2012071471A3 (en) Biosensing systems for measurement of lactose

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180212