JP2017537615A5 - - Google Patents

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JP2017537615A5
JP2017537615A5 JP2017525396A JP2017525396A JP2017537615A5 JP 2017537615 A5 JP2017537615 A5 JP 2017537615A5 JP 2017525396 A JP2017525396 A JP 2017525396A JP 2017525396 A JP2017525396 A JP 2017525396A JP 2017537615 A5 JP2017537615 A5 JP 2017537615A5
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cell
electric field
seconds
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cells
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JP2017525396A 2014-11-14 2015-11-13 破壊及び場による細胞への化合物及び組成物の送達 Active JP6846345B2 (ja)

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JP2021031352A JP7591265B2 (ja) 2014-11-14 2021-03-01 破壊及び場による細胞への化合物及び組成物の送達

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US201462080201P 2014-11-14 2014-11-14
US62/080,201 2014-11-14
US201562239241P 2015-10-08 2015-10-08
US62/239,241 2015-10-08
PCT/US2015/060689 WO2016077761A1 (en) 2014-11-14 2015-11-13 Disruption and field enabled delivery of compounds and compositions into cells

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JP2017537615A JP2017537615A (ja) 2017-12-21
JP2017537615A5 true JP2017537615A5 (https=) 2018-12-27
JP6846345B2 JP6846345B2 (ja) 2021-03-24

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JP2017525396A Active JP6846345B2 (ja) 2014-11-14 2015-11-13 破壊及び場による細胞への化合物及び組成物の送達
JP2021031352A Active JP7591265B2 (ja) 2014-11-14 2021-03-01 破壊及び場による細胞への化合物及び組成物の送達
JP2023002190A Pending JP2023036990A (ja) 2014-11-14 2023-01-11 破壊及び場による細胞への化合物及び組成物の送達

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JP2023002190A Pending JP2023036990A (ja) 2014-11-14 2023-01-11 破壊及び場による細胞への化合物及び組成物の送達

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US (1) US10526573B2 (https=)
EP (1) EP3218492A4 (https=)
JP (3) JP6846345B2 (https=)
CN (2) CN107002089B (https=)
CA (1) CA2964138C (https=)
WO (1) WO2016077761A1 (https=)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107058101B (zh) * 2011-10-17 2021-06-01 麻省理工学院 细胞内传递
EP2934532B1 (en) 2012-12-20 2019-10-23 Purdue Research Foundation Chimeric antigen receptor-expressing t cells as anti-cancer therapeutics
EP3033184B1 (en) 2013-08-16 2021-03-31 Massachusetts Institute of Technology Selective delivery of material to cells
JP7523203B2 (ja) 2014-10-31 2024-07-26 マサチューセッツ インスティテュート オブ テクノロジー 生体分子の免疫細胞への送達
WO2016077761A1 (en) 2014-11-14 2016-05-19 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
CN107250373A (zh) 2015-01-12 2017-10-13 麻省理工学院 通过微流体递送实现的基因编辑
SG10201912910PA (en) 2015-07-09 2020-02-27 Massachusetts Inst Technology Delivery of materials to anucleate cells
EP3344575B1 (en) 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
US20190275520A1 (en) 2016-03-31 2019-09-12 Massachusetts Institute Of Technology Flow-through microfluidic methods and devices featuring membrane-perturbing surface interactions for intracellular delivery
JP7282521B2 (ja) 2016-04-08 2023-05-29 パーデュー・リサーチ・ファウンデイション Ｃａｒ ｔ細胞療法のための方法および組成物
KR102490952B1 (ko) 2016-05-03 2023-01-19 에스큐지 바이오테크놀로지스 컴퍼니 관용을 유도하는 생체분자의 세포내 전달
JP7602702B2 (ja) 2016-05-03 2024-12-19 ステムセル テクノロジーズ カナダ インコーポレーテッド 寛容性を誘導するための生体分子の細胞内送達
US10724043B2 (en) * 2016-10-06 2020-07-28 Nantbio, Inc. Multi-pulse transfection methods and cells
CA3088495A1 (en) * 2017-02-13 2018-08-16 The Regents Of The University Of California Device and method for intracellular delivery of biomolecular cargo via acoustic wave exposure
JP7178355B2 (ja) 2017-02-28 2022-11-25 エンドサイト・インコーポレイテッド Ｃａｒ ｔ細胞療法のための組成物および方法
CN111315892B (zh) 2017-11-02 2024-03-12 因迪私人有限公司 细胞内递送及其方法
US11365390B2 (en) 2017-12-19 2022-06-21 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
JP7549303B2 (ja) 2018-01-22 2024-09-11 エンドサイト・インコーポレイテッド Ｃａｒ ｔ細胞の使用方法
CN112105382A (zh) 2018-02-23 2020-12-18 恩多塞特公司 用于car t细胞疗法的顺序方法
US12599656B2 (en) 2018-03-12 2026-04-14 Stemcell Technologies Canada Inc. Methods for treating HPV-associated diseases
EP3556845A1 (en) 2018-04-20 2019-10-23 Cellix Limited A method and device for transfecting cells
CN108887095B (zh) * 2018-05-15 2020-10-02 中亚民生科技发展有限公司 一种发光植物的培育方法
EP3820612A4 (en) * 2018-07-12 2022-05-04 GPB Scientific, Inc. MICROFLUID PROCESSES FOR CELL MANUFACTURE
US11052395B2 (en) * 2018-08-21 2021-07-06 The Regents Of The University Of California Lateral cavity acoustic transducer (LCAT) for shear-induced cell transfection
US12478965B2 (en) 2018-08-21 2025-11-25 The Regents Of The University Of California Cavity acoustic transducer (CAT) for shear-induced cell transfection
EP3844261A2 (en) * 2018-08-31 2021-07-07 The Charles Stark Draper Laboratory, Inc. Method and apparatus for high throughput high efficiency transfection of cells
WO2020096690A2 (en) * 2018-09-04 2020-05-14 The Charles Stark Draper Laboratory, Inc. Method and device for high field strength electrotransfection of microvescicles and cells
CN111202506A (zh) * 2018-11-21 2020-05-29 浙江清华柔性电子技术研究院 流体的检测器件及其制备方法、血管中血液的检测器件
KR102232757B1 (ko) * 2018-11-22 2021-03-26 (주)엑솔런스바이오테크놀로지 체외충격파를 이용한 표적물질 전달 장치
WO2020154696A1 (en) 2019-01-25 2020-07-30 Sqz Biotechnologies Company Anucleate cell-derived vaccines
CA3131701A1 (en) 2019-02-28 2020-09-03 Sqz Biotechnologies Company Delivery of biomolecules to pbmcs to modify an immune response
CN113840657B (zh) * 2019-03-12 2023-04-14 明新特生物科技公司 用于材料的细胞内输送的微流体系统及其方法
US11679388B2 (en) 2019-04-08 2023-06-20 Sqz Biotechnologies Company Cartridge for use in a system for delivery of a payload into a cell
US12465912B2 (en) 2019-04-11 2025-11-11 The Chinese University Of Hong Kong Systems and methods for controlled membrane disruption
US11685891B2 (en) 2019-05-17 2023-06-27 Regents Of The University Of Colorado Precise mechanical disruption for intracellular delivery to cells and small organisms
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
CN110272810A (zh) * 2019-07-01 2019-09-24 广州世赛生物科技有限公司 外源物质递送至真核细胞内的装置和方法及其应用
CA3145294A1 (en) * 2019-07-02 2021-01-07 Kytopen Corporation Devices, systems, and kits for electro-mechanical delivery and methods of use thereof
WO2021194497A1 (en) 2020-03-26 2021-09-30 Hewlett-Packard Development Company, L.P. Cell preparation with a series of detection devices
CN112268934B (zh) * 2020-09-16 2022-06-28 东南大学 一种针对循环肿瘤细胞的检测芯片及其检测方法
JP7642813B2 (ja) 2020-11-18 2025-03-10 セルフィー、インク． 生体細胞へのメカノポレーションベースのペイロード送達のための方法及びシステム
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
CA3202806A1 (en) 2020-12-29 2022-07-07 Maisam DADGAR High-throughput microfluidic chip having parallelized constrictions for perturbing cell membranes
CN114134028B (zh) * 2022-01-29 2022-05-10 季华实验室 细胞显微注射装置和控制方法
CN115254214B (zh) * 2022-06-29 2023-07-25 中国科学院精密测量科学与技术创新研究院 一种微流控通道、微流控芯片和生化分子递送方法
CN115308301B (zh) * 2022-08-16 2023-03-10 中山大学 一种可测量细胞及细胞核的弹性模量的测量装置
CN115927465B (zh) * 2022-12-26 2025-06-17 苏州依科赛生物科技股份有限公司 一种真核细胞的电转染方法
EP4428222A1 (en) * 2023-03-10 2024-09-11 Cambridge Enterprise, Ltd. Devices and methods for electroporation
KR102679742B1 (ko) * 2023-11-30 2024-07-01 (주)엠엑스티바이오텍 액적 기반 세포 내 물질 전달 플랫폼

Family Cites Families (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2502621C3 (de) 1975-01-23 1978-09-14 Kernforschungsanlage Juelich Gmbh, 5170 Juelich Messung elastischer und dielektrischer Eigenschaften der Membran lebender Zellen
US4478824A (en) 1983-08-08 1984-10-23 Franco Robert S Method for altering red blood cell function and survival
FR2569477B1 (fr) 1984-08-24 1987-01-02 Descartes Universite Rene Appareil et procede pour la determination de la deformabilite des globules rouges du sang
DE3742607A1 (de) 1987-12-16 1989-06-29 Asea Brown Boveri Hochtemperatur-speicherbatterie
JP2720161B2 (ja) 1988-02-01 1998-02-25 株式会社アドバンス 細胞変形能測定装置
JP2685544B2 (ja) 1988-11-11 1997-12-03 株式会社日立製作所 血液フィルタおよび血液検査方法並びに血液検査装置
JP2532707B2 (ja) 1990-03-08 1996-09-11 佑二 菊池 血液回路及びこれを用いた血液測定装置及び血液測定方法
US5643577A (en) 1990-04-24 1997-07-01 The University Of Newcastle Research Associates Limited Oral vaccine comprising antigen surface-associated with red blood cells
US5658892A (en) 1993-01-15 1997-08-19 The General Hospital Corporation Compound delivery using high-pressure impulse transients
US6218166B1 (en) 1994-12-09 2001-04-17 John Wayne Cancer Institute Adjuvant incorporation into antigen carrying cells: compositions and methods
JP3926842B2 (ja) 1995-03-08 2007-06-06 ザ・スクリプス・リサーチ・インステイチユート 抗原提示系およびｔ−細胞の活性化方法
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
DE69626853T2 (de) 1995-12-04 2004-02-19 Puget Sound Blood Center And Programm, Seattle Nicht-immunogene Blutplättchen und rote Blutzellen enthaltende Zubereitungen
US5951976A (en) 1996-03-28 1999-09-14 Whitenead Institute For Biomedical Research Opsonin-enhanced cells, and methods of modulating an immune response to an antigen
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
EP0882448B1 (en) 1997-05-05 2005-01-12 DIDECO S.r.l. Method of encapsulating biologically active agents within erythrocytes and apparatus therefor
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
SE9704076D0 (sv) 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
WO2003046170A1 (en) 2001-11-27 2003-06-05 Cellectricon Ab A method for combined parallel agent delivery and electroporation for cell structures and use thereof
GB9816583D0 (en) 1998-07-31 1998-09-30 Univ Ulster Nucleic acid carrier
WO2000078920A1 (en) 1999-06-21 2000-12-28 The General Hospital Corporation Methods and devices for cell culturing and organ assist systems
JP2002325572A (ja) 2000-12-25 2002-11-12 Univ Osaka 外来物質の導入方法
AU2002252073A1 (en) 2001-02-22 2002-09-12 The Scepens Eye Research Institute, Inc. Tolerogenic antigen presenting cells and in treating immune-inflammatory conditions
WO2003020039A1 (en) 2001-08-28 2003-03-13 Rush-Presbyterian-St. Luke's Medical Center Immune tolerance to predetermined antigens
US20030133922A1 (en) 2002-01-15 2003-07-17 Kasha John R. Oral tolerance using allogeneic platelets in ITP
US7148059B1 (en) * 2002-02-22 2006-12-12 Ionoptix Corporation Myocyte culture pacing apparatus
EP1411351A4 (en) 2002-06-05 2010-07-07 Panasonic Corp DEVICE FOR MEASURING THE EXTRACELLULAR POTENTIAL AND METHOD FOR PRODUCING THE DEVICE
GB0214528D0 (en) 2002-06-24 2002-08-07 Univ Aberdeen Materials and methods for induction of immune tolerance
US20040176282A1 (en) 2003-01-09 2004-09-09 Brian Dalby Cellular delivery and activation of polypeptide-nucleic acid complexes
US20060134067A1 (en) 2003-02-18 2006-06-22 Maxcyte, Inc. Loading of cells with antigens by electroporation
EP1771608B1 (en) 2004-07-27 2010-09-15 DSM IP Assets B.V. Process for making a carbon nanotubes/ultra-high molar mass polyethylene composite fibre
US20060134772A1 (en) 2004-11-18 2006-06-22 The Regents Of The University Of California System for locating cells and for cellular analysis
WO2006095330A2 (en) 2005-03-10 2006-09-14 Yeda Research And Development Co. Ltd. Methods and immunogenic cell preparations for treating antigen-associated diseases
US7704743B2 (en) 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
WO2007067032A1 (en) 2005-12-09 2007-06-14 Academisch Medisch Cemtrum Bij De Universiteit Van Amsterdam Means and methods for influencing the stability of cells
WO2007097934A2 (en) 2006-02-17 2007-08-30 Elusys Therapeutics, Inc. Methods and compositions for using erythrocytes as carriers for delivery of drugs
US8293524B2 (en) 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US20070249038A1 (en) 2006-04-21 2007-10-25 Andrea Adamo Microfluidic device for single cell targeted operations
AU2007284454A1 (en) 2006-08-17 2008-02-21 Massachusetts Institute Of Technology Method and apparatus for microfluidic injection
WO2008133755A2 (en) * 2007-01-05 2008-11-06 The Regents Of The University Of California Intracellular molecular delivery based on nanostructure injectors
US20080311140A1 (en) 2007-05-29 2008-12-18 Baylor College Of Medicine Antigen specific immunosuppression by dendritic cell therapy
US8841135B2 (en) 2007-06-20 2014-09-23 University Of Washington Biochip for high-throughput screening of circulating tumor cells
EP2169070B1 (en) 2007-06-21 2015-06-10 National University Corporation Nagoya University Method for introducing foreign substance into cell having cell wall
FR2919804B1 (fr) 2007-08-08 2010-08-27 Erytech Pharma Composition et vaccin therapeutique anti-tumoral
GB0718160D0 (en) 2007-09-18 2007-10-24 Medical Res Council Methods
EP2057998A1 (en) 2007-10-31 2009-05-13 Universitätsklinikum Hamburg-Eppendorf Use of modified cells for the treatment of multiple sclerosis
EP2216639A4 (en) 2007-11-28 2014-01-15 Konica Minolta Opto Inc APPARATUS AND METHOD FOR MEASURING BLOOD FLUIDITY
US20090280518A1 (en) 2008-05-12 2009-11-12 Massachusetts Institute Of Technology System for high throughput measurement of mechanical properties of cells
JP5137129B2 (ja) 2008-07-23 2013-02-06 国立大学法人山口大学 血液細胞の力学的特性測定装置
EP2321405A4 (en) 2008-08-08 2012-09-12 Agency Science Tech & Res Microfluidic continuous flow device
US8211656B2 (en) 2008-08-13 2012-07-03 The Invention Science Fund I, Llc Biological targeting compositions and methods of using the same
US8187864B2 (en) * 2008-10-01 2012-05-29 The Governing Council Of The University Of Toronto Exchangeable sheets pre-loaded with reagent depots for digital microfluidics
US20110091973A1 (en) 2008-12-09 2011-04-21 Glaser Larry F Modified and fusion enhanced erythrocytes, cells and uses thereof
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
US9017991B2 (en) 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
EP2405959A4 (en) 2009-03-13 2013-10-16 Univ Tufts METHODS, DEVICES AND KITS FOR INTRODUCING GENETIC MATERIAL IN LIVING CELLS
SG174373A1 (en) 2009-03-20 2011-10-28 Agency Science Tech & Res Devices for separating cells and methods of using them
WO2010129671A2 (en) * 2009-05-08 2010-11-11 Chauncey Sayre Method of and apparatus for changing one type of cell into another type of cell
GB0909754D0 (en) 2009-06-05 2009-07-22 Magnani Mauro Drug delivery systems
US20110300205A1 (en) 2009-07-06 2011-12-08 Novartis Ag Self replicating rna molecules and uses thereof
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
HRP20161223T1 (hr) 2009-10-27 2016-11-18 Erytech Pharma Pripravak za induciranje specifične imune tolerancije
CA2951341A1 (en) 2009-12-21 2011-06-30 Keygene N.V. Improved techniques for transfecting protoplasts
US9005579B2 (en) 2010-01-05 2015-04-14 Contrafect Corporation Methods and compositions for enhanced immunological therapy and targeting of gram-positive bacteria
US8844570B2 (en) 2010-02-01 2014-09-30 California Institute Of Technology Generating binary states using a microfluidic channel
JP5704590B2 (ja) 2010-02-05 2015-04-22 国立大学法人東京農工大学 サイズ選択マイクロキャビティアレイを用いた循環腫瘍細胞の検出
US20110293558A1 (en) 2010-03-22 2011-12-01 Massachusetts Institute Of Technology Material properties of t cells and related methods and compositions
WO2015061458A1 (en) 2013-10-22 2015-04-30 Cellanyx Diagnostics, Inc. Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells
EP2665540A1 (en) 2011-01-19 2013-11-27 The University of British Columbia Apparatus and method for particle separation
KR102117921B1 (ko) 2011-02-28 2020-06-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 세포 배양 시스템
CN102250842A (zh) * 2011-05-23 2011-11-23 昆明理工大学 一种电穿孔转染昆虫细胞的方法
WO2012162779A1 (en) 2011-05-27 2012-12-06 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
HRP20141230T1 (hr) 2011-07-05 2015-02-13 SOTIO a.s. Naäśini i postupci aktivne staniäśne imunoterapije raka upotrebom tumorskih stanica usmrä†enih visokim hidrostatskim tlakom, te dendritiäśkih stanica
US20130065314A1 (en) 2011-08-25 2013-03-14 Phytocentric Biotech Inc. Algal transformation systems, compositions and methods
CN107058101B (zh) * 2011-10-17 2021-06-01 麻省理工学院 细胞内传递
JP2015520194A (ja) 2012-06-07 2015-07-16 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 薬物標的指向化のためのナノ療法
US9255245B2 (en) 2012-07-03 2016-02-09 Agilent Technologies, Inc. Sample probes and methods for sampling intracellular material
US9327526B2 (en) * 2012-07-25 2016-05-03 Xerox Corporation Active biased electrodes for reducing electrostatic fields underneath print heads in an electrostatic media transport
CN102925337B (zh) 2012-11-08 2014-06-18 武汉友芝友生物制药有限公司 一种微流体细胞捕获芯片及其制备方法
GB201300049D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-stimulatory dendritic cells
GB201300052D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-suppressive dendritic cells
US20140295490A1 (en) 2013-01-30 2014-10-02 Asta Fluidic Technologies, Inc. Fetal red blood cell detection
US9725709B2 (en) 2013-03-12 2017-08-08 OpenCell Technologies, Inc. Intracellular delivery and transfection methods and devices
US10982217B2 (en) 2013-03-15 2021-04-20 The Regents Of The University Of California High-throughput cargo delivery into live cells using photothermal platforms
KR20140115560A (ko) 2013-03-21 2014-10-01 인하대학교 산학협력단 기계적 스트레스를 이용한 단세포 생물체 개질 방법 및 이에 사용되는 장치
AU2014248119B2 (en) 2013-04-03 2019-06-20 Memorial Sloan-Kettering Cancer Center Effective generation of tumor-targeted T-cells derived from pluripotent stem cells
EP3033184B1 (en) 2013-08-16 2021-03-31 Massachusetts Institute of Technology Selective delivery of material to cells
WO2015103331A1 (en) 2013-12-31 2015-07-09 Canon U.S. Life Sciences, Inc. Methods and systems for continuous flow cell lysis in a microfluidic device
ES2865825T3 (es) 2014-04-01 2021-10-18 Rubius Therapeutics Inc Procedimientos y composiciones para inmunomodulación
CA2945335A1 (en) 2014-04-18 2015-10-22 Editas Medicine, Inc. Crispr-cas-related methods, compositions and components for cancer immunotherapy
US10947526B2 (en) * 2014-07-03 2021-03-16 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
JP7523203B2 (ja) 2014-10-31 2024-07-26 マサチューセッツ インスティテュート オブ テクノロジー 生体分子の免疫細胞への送達
WO2016077761A1 (en) 2014-11-14 2016-05-19 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
WO2016109864A1 (en) 2015-01-07 2016-07-14 Indee. Inc. A method for mechanical and hydrodynamic microfluidic transfection and apparatus therefor
CN107250373A (zh) 2015-01-12 2017-10-13 麻省理工学院 通过微流体递送实现的基因编辑
US20180135012A1 (en) 2015-05-13 2018-05-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
GB201513278D0 (en) 2015-07-03 2015-09-09 Transimmune Ag And Yale University Device and method for obtaining immunostimulatory antigen-presenting cells
SG10201912910PA (en) 2015-07-09 2020-02-27 Massachusetts Inst Technology Delivery of materials to anucleate cells
EP3344575B1 (en) 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
ES2930017T3 (es) 2015-09-04 2022-12-05 Sqz Biotechnologies Co Suministro intracelular de biomoléculas mediado por una superficie con poros
CA3009369A1 (en) 2015-12-22 2017-06-29 Sangui Bio Pty. Ltd Therapeutic methods using erythrocytes
AU2017207736A1 (en) 2016-01-11 2018-07-12 Rubius Therapeutics, Inc. Compositions and methods related to multimodal therapeutic cell systems for immune indications
CN108779475A (zh) 2016-01-12 2018-11-09 Sqz生物技术公司 复合物的细胞内递送
KR102490952B1 (ko) 2016-05-03 2023-01-19 에스큐지 바이오테크놀로지스 컴퍼니 관용을 유도하는 생체분자의 세포내 전달
JP7602702B2 (ja) 2016-05-03 2024-12-19 ステムセル テクノロジーズ カナダ インコーポレーテッド 寛容性を誘導するための生体分子の細胞内送達
US20170326213A1 (en) 2016-05-16 2017-11-16 Augusta University Research Institute, Inc. Protein-Coupled Red Blood Cell Compositions and Methods of Their Use
CN106244543A (zh) 2016-07-29 2016-12-21 北京时合生物科技有限公司 一种pbmc体外诱导分化成为树突状细胞的方法
WO2018089497A1 (en) 2016-11-08 2018-05-17 Georgia Tech Research Corporation Methods for convectively-driven intracellular delivery

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