JP2017503521A5 - - Google Patents
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- Publication number
- JP2017503521A5 JP2017503521A5 JP2016548054A JP2016548054A JP2017503521A5 JP 2017503521 A5 JP2017503521 A5 JP 2017503521A5 JP 2016548054 A JP2016548054 A JP 2016548054A JP 2016548054 A JP2016548054 A JP 2016548054A JP 2017503521 A5 JP2017503521 A5 JP 2017503521A5
- Authority
- JP
- Japan
- Prior art keywords
- reverse transcriptase
- nucleic acid
- mutant
- mlv
- mlv reverse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102100034343 Integrase Human genes 0.000 claims description 190
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 187
- 230000000694 effects Effects 0.000 claims description 46
- 102000039446 nucleic acids Human genes 0.000 claims description 44
- 108020004707 nucleic acids Proteins 0.000 claims description 44
- 150000007523 nucleic acids Chemical class 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 32
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 31
- 230000035772 mutation Effects 0.000 claims description 27
- 239000000203 mixture Substances 0.000 claims description 23
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 16
- 230000000295 complement effect Effects 0.000 claims description 12
- 239000011692 calcium ascorbate Substances 0.000 claims description 9
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 238000001668 nucleic acid synthesis Methods 0.000 claims description 7
- 239000002773 nucleotide Substances 0.000 claims description 7
- 125000003729 nucleotide group Chemical group 0.000 claims description 7
- 108020004999 messenger RNA Proteins 0.000 claims description 6
- 102200054079 rs121907976 Human genes 0.000 claims description 6
- 102200070544 rs202198133 Human genes 0.000 claims description 6
- 102200111286 rs2234704 Human genes 0.000 claims description 6
- 102220242537 rs762217448 Human genes 0.000 claims description 6
- 102200078515 rs779270933 Human genes 0.000 claims description 6
- 239000005546 dideoxynucleotide Substances 0.000 claims description 4
- 229920001184 polypeptide Polymers 0.000 claims description 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 238000010839 reverse transcription Methods 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 101710203526 Integrase Proteins 0.000 claims description 3
- 239000013604 expression vector Substances 0.000 claims description 2
- 102200028520 rs1057520297 Human genes 0.000 claims description 2
- 239000013598 vector Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000004113 Sepiolite Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 239000003419 rna directed dna polymerase inhibitor Substances 0.000 description 2
- 102220288227 rs141863326 Human genes 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461930395P | 2014-01-22 | 2014-01-22 | |
| US61/930,395 | 2014-01-22 | ||
| PCT/US2015/012534 WO2015112767A2 (en) | 2014-01-22 | 2015-01-22 | Novel reverse transcriptases for use in high temperature nucleic acid synthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017503521A JP2017503521A (ja) | 2017-02-02 |
| JP2017503521A5 true JP2017503521A5 (OSRAM) | 2018-02-01 |
Family
ID=52444678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016548054A Pending JP2017503521A (ja) | 2014-01-22 | 2015-01-22 | 高温核酸合成で使用するための新規な逆転写酵素 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9663770B2 (OSRAM) |
| EP (1) | EP3097190A2 (OSRAM) |
| JP (1) | JP2017503521A (OSRAM) |
| KR (1) | KR20160113177A (OSRAM) |
| CN (1) | CN106164261A (OSRAM) |
| WO (1) | WO2015112767A2 (OSRAM) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| WO2016033116A1 (en) * | 2014-08-25 | 2016-03-03 | Life Technologies Corporation | Reversibly inactivated thermostable reverse transcriptases, compositions and methods for use |
| EP3365356B1 (en) | 2015-10-23 | 2023-06-28 | President and Fellows of Harvard College | Nucleobase editors and uses thereof |
| JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US9580698B1 (en) * | 2016-09-23 | 2017-02-28 | New England Biolabs, Inc. | Mutant reverse transcriptase |
| EP3516050B1 (en) | 2016-09-23 | 2021-08-11 | New England Biolabs, Inc. | A mutant reverse transcriptase |
| US11220677B2 (en) | 2016-12-14 | 2022-01-11 | Takara Bio Inc. | Heat-resistant reverse transcriptase mutant |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| WO2018165631A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Cancer vaccine |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| CN110709513B (zh) * | 2017-04-11 | 2023-10-03 | 豪夫迈·罗氏有限公司 | 具有热稳定性增加的突变型逆转录酶以及涉及其的产物、方法和用途 |
| US20180312822A1 (en) * | 2017-04-26 | 2018-11-01 | 10X Genomics, Inc. | Mmlv reverse transcriptase variants |
| WO2018200867A1 (en) * | 2017-04-26 | 2018-11-01 | 10X Genomics, Inc. | Mmlv reverse transcriptase variants |
| WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| CN113785053B (zh) * | 2018-12-26 | 2024-07-12 | 深圳华大生命科学研究院 | 酶活性提高的逆转录酶及其应用 |
| WO2020154500A1 (en) | 2019-01-23 | 2020-07-30 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| MX2021011426A (es) | 2019-03-19 | 2022-03-11 | Broad Inst Inc | Metodos y composiciones para editar secuencias de nucleótidos. |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| JP7779828B2 (ja) * | 2019-07-26 | 2025-12-03 | ベクトン・ディキンソン・アンド・カンパニー | 凝集を低減するための緩衝剤組成物 |
| US20220290112A1 (en) * | 2019-07-26 | 2022-09-15 | Toyobo Co., Ltd. | Variant reverse transcriptase exhibiting excellent stability |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| CN112795547B (zh) * | 2019-11-13 | 2024-05-31 | 广州达安基因股份有限公司 | 高逆转录效率的逆转录酶突变体 |
| CN112795551B (zh) * | 2019-11-13 | 2024-01-30 | 广州达安基因股份有限公司 | 一种耐高温逆转录酶突变体及其应用 |
| CN112795546B (zh) * | 2019-11-13 | 2024-02-27 | 广州达安基因股份有限公司 | 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用 |
| CN112795549B (zh) * | 2019-11-13 | 2023-11-28 | 广州达安基因股份有限公司 | 一种逆转录酶突变体 |
| CN112795550B (zh) * | 2019-11-13 | 2024-05-31 | 广州达安基因股份有限公司 | 耐高温逆转录酶突变体 |
| WO2021119320A2 (en) | 2019-12-11 | 2021-06-17 | 10X Genomics, Inc. | Reverse transcriptase variants |
| CN115768558A (zh) | 2020-01-13 | 2023-03-07 | 福路伦特生物科学公司 | 用于单细胞基因谱分析的方法和系统 |
| JP2023511279A (ja) | 2020-01-13 | 2023-03-17 | フルーエント バイオサイエンシーズ インコーポレイテッド | 単一細胞シーケンシング |
| WO2021146183A1 (en) * | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Reverse transcription during template emulsification |
| US11512337B2 (en) | 2020-01-13 | 2022-11-29 | Fluent Biosciences Inc. | Emulsion based drug screening |
| JP2023515499A (ja) * | 2020-02-21 | 2023-04-13 | バイオオー サイエンティフィック コーポレーション | 超並列核酸シーケンシングライブラリを生成するための組成物及び方法 |
| IL296024A (en) | 2020-03-04 | 2022-10-01 | Flagship Pioneering Innovations Vi Llc | Methods and compositions for modulating a genome |
| US11866782B2 (en) | 2020-03-16 | 2024-01-09 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| US12428685B2 (en) | 2020-03-24 | 2025-09-30 | Illumina, Inc. | Viral detection using template emulsification |
| MX2022014008A (es) | 2020-05-08 | 2023-02-09 | Broad Inst Inc | Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo. |
| CN111647576B (zh) * | 2020-06-24 | 2021-02-09 | 南京诺唯赞生物科技股份有限公司 | 一种热稳定性逆转录酶突变体及其应用 |
| KR102338690B1 (ko) * | 2020-07-09 | 2021-12-14 | (주)바이오닉스 | 항체결합단백질 및 우라실 dna 글리코실레이즈 효소를 포함하는 융합 단백질, 및 상기 융합 단백질의 용도 |
| CA3189597A1 (en) | 2020-07-15 | 2022-01-20 | Fluent Biosciences Inc. | Tiered ligation oligos |
| WO2022020371A2 (en) * | 2020-07-20 | 2022-01-27 | Integrated Dna Technologies, Inc. | Reverse transcriptase mutants with increased activity and thermostability |
| US20220135955A1 (en) * | 2020-07-20 | 2022-05-05 | Integrated DNA Techonolgies, Inc. | Reverse Transcriptase Mutants with Increased Activity and Thermostability |
| CN114480335B (zh) * | 2020-11-13 | 2024-06-07 | 广州达安基因股份有限公司 | 逆转录酶及逆转录检测试剂 |
| CN114480331A (zh) * | 2020-11-13 | 2022-05-13 | 广州达安基因股份有限公司 | Mmlv酶组合突变体 |
| CN114480330B (zh) * | 2020-11-13 | 2024-11-01 | 广州达安基因股份有限公司 | 多突变位点的逆转录酶突变体 |
| CN114480338B (zh) * | 2020-11-13 | 2024-04-05 | 广州达安基因股份有限公司 | 用于核酸检测的逆转录酶突变体 |
| CN112251423B (zh) * | 2020-12-08 | 2021-03-19 | 北京健为医学检验实验室有限公司 | 一种m-mlv逆转录酶体及其应用 |
| CN113234858A (zh) * | 2021-05-20 | 2021-08-10 | 阿吉安(福州)基因医学检验实验室有限公司 | 一种covid-19的检测试剂盒 |
| CN113355303B (zh) * | 2021-06-07 | 2023-06-16 | 中元汇吉生物技术股份有限公司 | 一种m-mlv逆转录酶突变体及其应用 |
| AU2022343268A1 (en) * | 2021-09-08 | 2024-03-28 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| CN117586983A (zh) * | 2022-08-11 | 2024-02-23 | 菲鹏生物股份有限公司 | 一种逆转录酶、核酸分子及合成cDNA的方法 |
| CN120359295A (zh) * | 2022-12-21 | 2025-07-22 | 成对植物服务股份有限公司 | 工程化蛋白质和其使用方法 |
| CN116004568B (zh) * | 2023-02-07 | 2025-10-17 | 北京诺派生物科技有限公司 | 一种可用于核酸检测的耐高温反转录酶及其制备方法与应用 |
| CN116286721A (zh) * | 2023-03-29 | 2023-06-23 | 江苏同科医药科技有限公司 | 一种耐热型逆转录酶突变体及其制备方法和应用 |
| CN116286723A (zh) * | 2023-04-28 | 2023-06-23 | 深圳市艾伟迪生物科技有限公司 | 一种基于蛋白定向进化的快速逆转录酶及其应用 |
| GB2630305A (en) * | 2023-05-22 | 2024-11-27 | Thermo Fisher Scientific Baltics Uab | Reverse transcriptase with improved properties |
| CN119506243A (zh) * | 2023-06-30 | 2025-02-25 | 翌圣生物科技(上海)股份有限公司 | 一种鼠白血病病毒逆转录酶突变体及其应用 |
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| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| JPH0779694B2 (ja) | 1985-07-09 | 1995-08-30 | カドラント バイオリソ−シズ リミテツド | 蛋白質および同類品の保護 |
| US5693517A (en) | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5561058A (en) | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
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| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5498523A (en) | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
| GB8903593D0 (en) | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
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| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
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| CA2617790A1 (en) * | 2005-08-10 | 2007-02-22 | Stratagene California | Mutant reverse transcriptase and methods of use |
| GB0806562D0 (en) | 2008-04-10 | 2008-05-14 | Fermentas Uab | Production of nucleic acid |
| US8900814B2 (en) * | 2010-08-13 | 2014-12-02 | Kyoto University | Variant reverse transcriptase |
-
2015
- 2015-01-22 KR KR1020167022831A patent/KR20160113177A/ko not_active Withdrawn
- 2015-01-22 WO PCT/US2015/012534 patent/WO2015112767A2/en not_active Ceased
- 2015-01-22 US US14/603,256 patent/US9663770B2/en active Active
- 2015-01-22 EP EP15702365.6A patent/EP3097190A2/en not_active Withdrawn
- 2015-01-22 JP JP2016548054A patent/JP2017503521A/ja active Pending
- 2015-01-22 CN CN201580014630.7A patent/CN106164261A/zh active Pending
-
2017
- 2017-05-26 US US15/607,247 patent/US20180010105A1/en not_active Abandoned
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