JP2017503521A - 高温核酸合成で使用するための新規な逆転写酵素 - Google Patents

高温核酸合成で使用するための新規な逆転写酵素 Download PDF

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JP2017503521A
JP2017503521A JP2016548054A JP2016548054A JP2017503521A JP 2017503521 A JP2017503521 A JP 2017503521A JP 2016548054 A JP2016548054 A JP 2016548054A JP 2016548054 A JP2016548054 A JP 2016548054A JP 2017503521 A JP2017503521 A JP 2017503521A
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reverse transcriptase
mutant
mlv
nucleic acid
activity
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JP2017503521A5 (OSRAM
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ジェフリー ロジャーズ
ジェフリー ロジャーズ
ジェイソン ポッター
ジェイソン ポッター
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ライフ テクノロジーズ コーポレーション
ライフ テクノロジーズ コーポレーション
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)

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  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2016548054A 2014-01-22 2015-01-22 高温核酸合成で使用するための新規な逆転写酵素 Pending JP2017503521A (ja)

Applications Claiming Priority (3)

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US201461930395P 2014-01-22 2014-01-22
US61/930,395 2014-01-22
PCT/US2015/012534 WO2015112767A2 (en) 2014-01-22 2015-01-22 Novel reverse transcriptases for use in high temperature nucleic acid synthesis

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JP2017503521A true JP2017503521A (ja) 2017-02-02
JP2017503521A5 JP2017503521A5 (OSRAM) 2018-02-01

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US (2) US9663770B2 (OSRAM)
EP (1) EP3097190A2 (OSRAM)
JP (1) JP2017503521A (OSRAM)
KR (1) KR20160113177A (OSRAM)
CN (1) CN106164261A (OSRAM)
WO (1) WO2015112767A2 (OSRAM)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102338690B1 (ko) * 2020-07-09 2021-12-14 (주)바이오닉스 항체결합단백질 및 우라실 dna 글리코실레이즈 효소를 포함하는 융합 단백질, 및 상기 융합 단백질의 용도
JP2023515499A (ja) * 2020-02-21 2023-04-13 バイオオー サイエンティフィック コーポレーション 超並列核酸シーケンシングライブラリを生成するための組成物及び方法
JP2023517187A (ja) * 2020-03-04 2023-04-24 フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー ゲノムを調節するための方法及び組成物

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
WO2016033116A1 (en) * 2014-08-25 2016-03-03 Life Technologies Corporation Reversibly inactivated thermostable reverse transcriptases, compositions and methods for use
EP3365356B1 (en) 2015-10-23 2023-06-28 President and Fellows of Harvard College Nucleobase editors and uses thereof
JP7231935B2 (ja) 2016-08-03 2023-03-08 プレジデント アンド フェローズ オブ ハーバード カレッジ アデノシン核酸塩基編集因子およびそれらの使用
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US9580698B1 (en) * 2016-09-23 2017-02-28 New England Biolabs, Inc. Mutant reverse transcriptase
EP3516050B1 (en) 2016-09-23 2021-08-11 New England Biolabs, Inc. A mutant reverse transcriptase
US11220677B2 (en) 2016-12-14 2022-01-11 Takara Bio Inc. Heat-resistant reverse transcriptase mutant
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165631A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Cancer vaccine
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
CN110709513B (zh) * 2017-04-11 2023-10-03 豪夫迈·罗氏有限公司 具有热稳定性增加的突变型逆转录酶以及涉及其的产物、方法和用途
US20180312822A1 (en) * 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
WO2018200867A1 (en) * 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
CN113785053B (zh) * 2018-12-26 2024-07-12 深圳华大生命科学研究院 酶活性提高的逆转录酶及其应用
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
MX2021011426A (es) 2019-03-19 2022-03-11 Broad Inst Inc Metodos y composiciones para editar secuencias de nucleótidos.
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
JP7779828B2 (ja) * 2019-07-26 2025-12-03 ベクトン・ディキンソン・アンド・カンパニー 凝集を低減するための緩衝剤組成物
US20220290112A1 (en) * 2019-07-26 2022-09-15 Toyobo Co., Ltd. Variant reverse transcriptase exhibiting excellent stability
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN112795547B (zh) * 2019-11-13 2024-05-31 广州达安基因股份有限公司 高逆转录效率的逆转录酶突变体
CN112795551B (zh) * 2019-11-13 2024-01-30 广州达安基因股份有限公司 一种耐高温逆转录酶突变体及其应用
CN112795546B (zh) * 2019-11-13 2024-02-27 广州达安基因股份有限公司 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用
CN112795549B (zh) * 2019-11-13 2023-11-28 广州达安基因股份有限公司 一种逆转录酶突变体
CN112795550B (zh) * 2019-11-13 2024-05-31 广州达安基因股份有限公司 耐高温逆转录酶突变体
WO2021119320A2 (en) 2019-12-11 2021-06-17 10X Genomics, Inc. Reverse transcriptase variants
CN115768558A (zh) 2020-01-13 2023-03-07 福路伦特生物科学公司 用于单细胞基因谱分析的方法和系统
JP2023511279A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞シーケンシング
WO2021146183A1 (en) * 2020-01-13 2021-07-22 Fluent Biosciences Inc. Reverse transcription during template emulsification
US11512337B2 (en) 2020-01-13 2022-11-29 Fluent Biosciences Inc. Emulsion based drug screening
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.
CN111647576B (zh) * 2020-06-24 2021-02-09 南京诺唯赞生物科技股份有限公司 一种热稳定性逆转录酶突变体及其应用
CA3189597A1 (en) 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
WO2022020371A2 (en) * 2020-07-20 2022-01-27 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
US20220135955A1 (en) * 2020-07-20 2022-05-05 Integrated DNA Techonolgies, Inc. Reverse Transcriptase Mutants with Increased Activity and Thermostability
CN114480335B (zh) * 2020-11-13 2024-06-07 广州达安基因股份有限公司 逆转录酶及逆转录检测试剂
CN114480331A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 Mmlv酶组合突变体
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CN113355303B (zh) * 2021-06-07 2023-06-16 中元汇吉生物技术股份有限公司 一种m-mlv逆转录酶突变体及其应用
AU2022343268A1 (en) * 2021-09-08 2024-03-28 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
CN117586983A (zh) * 2022-08-11 2024-02-23 菲鹏生物股份有限公司 一种逆转录酶、核酸分子及合成cDNA的方法
CN120359295A (zh) * 2022-12-21 2025-07-22 成对植物服务股份有限公司 工程化蛋白质和其使用方法
CN116004568B (zh) * 2023-02-07 2025-10-17 北京诺派生物科技有限公司 一种可用于核酸检测的耐高温反转录酶及其制备方法与应用
CN116286721A (zh) * 2023-03-29 2023-06-23 江苏同科医药科技有限公司 一种耐热型逆转录酶突变体及其制备方法和应用
CN116286723A (zh) * 2023-04-28 2023-06-23 深圳市艾伟迪生物科技有限公司 一种基于蛋白定向进化的快速逆转录酶及其应用
GB2630305A (en) * 2023-05-22 2024-11-27 Thermo Fisher Scientific Baltics Uab Reverse transcriptase with improved properties
CN119506243A (zh) * 2023-06-30 2025-02-25 翌圣生物科技(上海)股份有限公司 一种鼠白血病病毒逆转录酶突变体及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005538719A (ja) * 2002-09-13 2005-12-22 インヴィトロジェン コーポレーション 耐熱性逆転写酵素およびその使用法
JP2009504162A (ja) * 2005-08-10 2009-02-05 ストラタジーン カリフォルニア 変異体逆転写酵素および使用方法
US20110081704A1 (en) * 2000-05-26 2011-04-07 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
JP2011516072A (ja) * 2008-04-10 2011-05-26 フェルメンタス ユーエービー 核酸の生成

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
JPH0779694B2 (ja) 1985-07-09 1995-08-30 カドラント バイオリソ−シズ リミテツド 蛋白質および同類品の保護
US5693517A (en) 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5561058A (en) 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US4962022A (en) 1986-09-22 1990-10-09 Becton Dickinson And Company Storage and use of liposomes
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5646016A (en) 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
RU2182176C2 (ru) 1991-09-24 2002-05-10 Кейгене Н.В. Способ селективной амплификации, олигонуклеотид и набор для селективной амплификации
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6015668A (en) 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5948614A (en) 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US6013488A (en) 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
EP2184289A1 (en) 1997-04-22 2010-05-12 Life Technologies Corporation Methods for the production of ASLV reverse transcriptases composed of multiple subunits
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
JP4193079B2 (ja) 1998-11-10 2008-12-10 東洋紡績株式会社 変異型逆転写酵素
CA2401417A1 (en) 2000-03-15 2001-09-20 Invitrogen Corporation High fidelity reverse transcriptases and uses thereof
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
EP1290149A4 (en) * 2000-05-26 2004-03-17 Invitrogen Corp THERMOSTABLE INVERTED TRANSCRIPTASES AND USES
WO2003025132A2 (en) 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
WO2006081222A2 (en) 2005-01-25 2006-08-03 Compass Genetics, Llc. Isothermal dna amplification
US8900814B2 (en) * 2010-08-13 2014-12-02 Kyoto University Variant reverse transcriptase

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110081704A1 (en) * 2000-05-26 2011-04-07 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
JP2005538719A (ja) * 2002-09-13 2005-12-22 インヴィトロジェン コーポレーション 耐熱性逆転写酵素およびその使用法
JP2009504162A (ja) * 2005-08-10 2009-02-05 ストラタジーン カリフォルニア 変異体逆転写酵素および使用方法
JP2011516072A (ja) * 2008-04-10 2011-05-26 フェルメンタス ユーエービー 核酸の生成

Cited By (4)

* Cited by examiner, † Cited by third party
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JP2023515499A (ja) * 2020-02-21 2023-04-13 バイオオー サイエンティフィック コーポレーション 超並列核酸シーケンシングライブラリを生成するための組成物及び方法
JP2023517187A (ja) * 2020-03-04 2023-04-24 フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー ゲノムを調節するための方法及び組成物
US12454706B2 (en) 2020-03-04 2025-10-28 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
KR102338690B1 (ko) * 2020-07-09 2021-12-14 (주)바이오닉스 항체결합단백질 및 우라실 dna 글리코실레이즈 효소를 포함하는 융합 단백질, 및 상기 융합 단백질의 용도

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CN106164261A (zh) 2016-11-23
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