JP2016116507A - 食品変敗微生物を検出するための組成物 - Google Patents
食品変敗微生物を検出するための組成物 Download PDFInfo
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- JP2016116507A JP2016116507A JP2015233650A JP2015233650A JP2016116507A JP 2016116507 A JP2016116507 A JP 2016116507A JP 2015233650 A JP2015233650 A JP 2015233650A JP 2015233650 A JP2015233650 A JP 2015233650A JP 2016116507 A JP2016116507 A JP 2016116507A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
[0031]本実施例では、本発明の核酸を使用するL.カゼイ(casei)の定量的検出を行う。
[0040]本実施例では本発明の核酸の特異性を明らかにする。
Claims (12)
- 配列番号1〜45からなる群から選択されるヌクレオチド配列からなる核酸。
- 2つ以上の核酸を含む核酸集団であって、前記2つ以上の核酸は各々、配列番号1〜45からなる群から選択されるヌクレオチド配列からなる、集団。
- 前記2つ以上の核酸は2つ以上の異なる核酸を含む、請求項2に記載の集団。
- 核酸集団を含むアッセイキットであって、前記集団は、
(a)配列番号1〜3、
(b)配列番号4〜6、
(c)配列番号7〜9、
(d)配列番号10〜12、
(e)配列番号13〜15、
(f)配列番号16〜18、
(g)配列番号19〜21、
(h)配列番号22、24及び27、
(i)配列番号22、25及び27、
(j)配列番号23、26及び27、
(k)配列番号28〜30、
(l)配列番号31〜33、
(m)配列番号34〜36、
(n)配列番号37〜39、
(o)配列番号40〜42、及び
(p)配列番号43〜45
からなる群から選択される、キット。 - 支持体上に固定化された請求項1に記載の核酸、請求項2又は3に記載の核酸集団又は請求項4に記載のアッセイキットの核酸集団を含む支持体。
- 検出可能な標識をさらに含む、請求項5に記載の支持体。
- 食品中の1又は複数の微生物の存在を検出するための方法であって、
(a)食品から単離された微生物核酸を含む少なくとも1つの試験サンプルを得るステップ、
(b)請求項1に記載の核酸、請求項2又は3に記載の核酸集団、請求項4に記載のアッセイキットの核酸集団、又は請求項5又は6に記載の核酸を含む支持体を、該核酸と前記微生物核酸との間で複合体が形成されるのを可能にする条件下で前記少なくとも1つの試験サンプルと接触させるステップ、
(c)前記複合体を検出するステップ、及び、任意選択で、
(d)前記少なくとも1つの試験サンプル中の複合体の量を、微生物核酸を欠くネガティブサンプルからの複合体の量と比較するステップ(該ステップにおいて、前記少なくとも1つの試験サンプルからの複合体の量の増加は1又は複数の微生物の存在を示す。)
を含む方法。 - 食品の変敗を引き起こす1又は複数の微生物の存在を検出するステップを含む、請求項7に記載の方法。
- ペディオコッカス(Pediococcus)、ラクトバチルス(Lactobacillus)、ペクチナタス(Pectinatus)及びメガスフェラ(Megasphaera)からなる群から選択される1又は複数の属の1又は複数の微生物の存在を検出するステップを含む、請求項7又は8に記載の方法。
- ペディオコッカス・ダムノサス(Pediococcus damnosus)、ペディオコッカス・イノピナタス(Pediococcus inopinatus)、ペディオコッカス・クラウセニ(Pediococcus claussenii)、ラクトバチルス・バッキ(Lactobacillus backii)、ラクトバチルス・ブレビス(Lactobacillus brevis)、ラクトバチルス・カゼイ(Lactobacillus casei)、ラクトバチルス・コリニフォルミス(Lactobacillus coryniformis)、ラクトバチルス・コリノイデス(Lactobacillus collinoides)、ラクトバチルス・リンドネリ(Lactobacillus lindneri)、ラクトバチルス・ロシエ(Lactobacillus rossiae)、ラクトバチルス・パラブフネリ(Lactobacillus parabuchneri)、ラクトバチルス・パラコリノイデス(Lactobacillus paracollinoides)、ラクトバチルス・ペロレンス(Lactobacillus perolens)、ラクトバチルス・プランタルム(Lactobacillus plantarum)、ペクチナタス・セレビシフィラス(Pectinatus cerevisiiphilus)、ペクチナタス・フリシンゲンシス(Pectinatus frisingensis)、ペクチナタス・ハイカラエ(Pectinatus haikarae)、ペクチナタス・ポルタレンシス(Pectinatus portalensis)、メガスフェラ・セレビシエ(Megasphaera cerevisiae)及びメガスフェラ・エルスデニ(Megasphaera elsdenii)からなる群から選択される1又は複数の微生物の存在を検出するステップを含む、請求項7〜9のいずれか一項に記載の方法。
- 食品が飲料である、請求項7〜10のいずれか一項に記載の方法。
- 食品がビールである、請求項7〜11のいずれか一項に記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/555,399 US9303281B2 (en) | 2012-07-23 | 2012-07-23 | Compositions for detecting foodstuff spoilage microorganisms |
US13/555,399 | 2012-07-23 |
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JP2013152725A Division JP6051430B2 (ja) | 2012-07-23 | 2013-07-23 | 食品変敗微生物を検出するための組成物 |
Publications (2)
Publication Number | Publication Date |
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JP2016116507A true JP2016116507A (ja) | 2016-06-30 |
JP6236690B2 JP6236690B2 (ja) | 2017-11-29 |
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Application Number | Title | Priority Date | Filing Date |
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JP2013152725A Expired - Fee Related JP6051430B2 (ja) | 2012-07-23 | 2013-07-23 | 食品変敗微生物を検出するための組成物 |
JP2015233650A Expired - Fee Related JP6236690B2 (ja) | 2012-07-23 | 2015-11-30 | 食品変敗微生物を検出するための組成物 |
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JP2013152725A Expired - Fee Related JP6051430B2 (ja) | 2012-07-23 | 2013-07-23 | 食品変敗微生物を検出するための組成物 |
Country Status (10)
Country | Link |
---|---|
US (1) | US9303281B2 (ja) |
EP (1) | EP2690179B1 (ja) |
JP (2) | JP6051430B2 (ja) |
KR (1) | KR101524259B1 (ja) |
CN (1) | CN103571829A (ja) |
AU (1) | AU2013206376A1 (ja) |
BR (1) | BR102013018581A2 (ja) |
CA (1) | CA2820041C (ja) |
SG (1) | SG196715A1 (ja) |
TW (1) | TWI502105B (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018224561A1 (en) * | 2017-06-07 | 2018-12-13 | Original G B.V. | Method for detecting food spoilage microbes |
CN111560313A (zh) * | 2020-05-14 | 2020-08-21 | 保睿(上海)生物科技有限公司 | 一种用于肠菌微生物的检测试剂盒及其检测方法 |
CN116064301A (zh) * | 2022-09-22 | 2023-05-05 | 广西大学 | 一株类布氏乳杆菌及其应用 |
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JP2007259730A (ja) * | 2006-03-28 | 2007-10-11 | Asahi Breweries Ltd | マルチプレックスpcr用人工陽性対照dna群の作製法 |
JP2008263971A (ja) * | 2007-03-26 | 2008-11-06 | Kirin Brewery Co Ltd | ラクトバチラス属乳酸菌の検出用プライマーおよびプローブ |
JP2010081935A (ja) * | 2008-09-02 | 2010-04-15 | Kirin Brewery Co Ltd | ペディオコッカス・ダムノサスの検出用プライマーおよびプローブ |
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-
2012
- 2012-07-23 US US13/555,399 patent/US9303281B2/en not_active Expired - Fee Related
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2013
- 2013-06-18 SG SG2013046982A patent/SG196715A1/en unknown
- 2013-06-18 AU AU2013206376A patent/AU2013206376A1/en not_active Abandoned
- 2013-06-19 EP EP13172688.7A patent/EP2690179B1/en not_active Not-in-force
- 2013-07-04 TW TW102124046A patent/TWI502105B/zh not_active IP Right Cessation
- 2013-07-04 CA CA2820041A patent/CA2820041C/en not_active Expired - Fee Related
- 2013-07-19 BR BRBR102013018581-7A patent/BR102013018581A2/pt not_active IP Right Cessation
- 2013-07-22 CN CN201310306778.0A patent/CN103571829A/zh active Pending
- 2013-07-23 KR KR1020130086759A patent/KR101524259B1/ko not_active IP Right Cessation
- 2013-07-23 JP JP2013152725A patent/JP6051430B2/ja not_active Expired - Fee Related
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- 2015-11-30 JP JP2015233650A patent/JP6236690B2/ja not_active Expired - Fee Related
Patent Citations (4)
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JP2007259730A (ja) * | 2006-03-28 | 2007-10-11 | Asahi Breweries Ltd | マルチプレックスpcr用人工陽性対照dna群の作製法 |
JP2008263971A (ja) * | 2007-03-26 | 2008-11-06 | Kirin Brewery Co Ltd | ラクトバチラス属乳酸菌の検出用プライマーおよびプローブ |
JP2010081935A (ja) * | 2008-09-02 | 2010-04-15 | Kirin Brewery Co Ltd | ペディオコッカス・ダムノサスの検出用プライマーおよびプローブ |
Also Published As
Publication number | Publication date |
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EP2690179B1 (en) | 2017-08-09 |
TW201407009A (zh) | 2014-02-16 |
US20140024543A1 (en) | 2014-01-23 |
JP2014027933A (ja) | 2014-02-13 |
EP2690179A1 (en) | 2014-01-29 |
TWI502105B (zh) | 2015-10-01 |
KR20140012903A (ko) | 2014-02-04 |
BR102013018581A2 (pt) | 2015-08-11 |
SG196715A1 (en) | 2014-02-13 |
KR101524259B1 (ko) | 2015-05-29 |
JP6051430B2 (ja) | 2016-12-27 |
US9303281B2 (en) | 2016-04-05 |
CA2820041A1 (en) | 2014-01-23 |
AU2013206376A1 (en) | 2014-02-06 |
CA2820041C (en) | 2016-04-12 |
JP6236690B2 (ja) | 2017-11-29 |
CN103571829A (zh) | 2014-02-12 |
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