JP2015510396A - マクロビーズ製造のための改良方法 - Google Patents
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- B25—HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
- B25J—MANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
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Abstract
Description
当該分野で非常に望ましい一改良は、手作業によるプロセスを自動化できることである。
粉末化アガロース(リテックスHSB−LV)を最小必須培地に溶解して濃度を0.8%又は4.5%(w/v)とした後、121℃で20分間オートクレーブ処理し、粘稠性融解溶液を得た。このアガロース溶液を冷却し、51〜53℃又は61〜63℃でそれぞれ維持した。
上述の温度勾配を用い、一連の異なる実験を行った。
鉱油温度勾配の最適開始温度が約25℃、終了温度が約−2℃であることが確立されたことを考慮し、これを一連の実験に用いて代謝活性及び腫瘍抑制能を測定した。
ビーズの製造を、上述のように本発明に従って行うと共に、引用した先行技術に記載の手作業による方法によって行った。7ヶ月間に亘り、10個のビーズを無作為に選択してその直径を測定した。次の表に示す結果から、本発明に従って製造したビーズは直径がより一定であり、即ち、コーティングがより一定であることが分かる。
次の実施例では、アガロースコートの膵島含有アガロースビーズ(3グループ)の製造について詳述する。
先の実施例に記載のように、サンプルから培地サンプルを採取し、市販のブタインスリンELISAを用いてインスリン含量についてアッセイした。結果を図6に示す。インスリン産生に関しては、0.8%SG及び1.5%SGのいずれかにカプセル化された膵島よりも0.8%Liにカプセル化された膵島の方が優れていることが分かる。
上述のインビトロ実験を本明細書に記載のインビボ実験に拡張した。
レシピエントラットを屠殺した後、マクロビーズに関して研究を行い、構造的完全性や機能的能力について検討した。
Claims (16)
- アガロースでコートされたアガロース含有ビーズ中に細胞サンプルを含む組成物を製造する方法であって、
(a)第1のアガロースサンプルを前記細胞と混合して懸濁液を形成することと、
(b)前記懸濁液からビーズを形成することと、
(c)前記ビーズを第2のアガロース溶液でコートすることと、
(d)前記コートビーズを鉱油サンプルに分注することとを含む方法において、前記鉱油サンプルを温度勾配下に維持して、前記ビーズが高温の鉱油から低温の鉱油へ移動するようにする方法。 - 前記細胞は分泌細胞である、請求項1に記載の方法。
- 前記細胞は癌細胞である、請求項1に記載の方法。
- 前記細胞は癌幹細胞である、請求項1に記載の方法。
- 前記細胞は膵島細胞である、請求項1に記載の方法。
- 前記細胞は幹細胞である、請求項1に記載の方法。
- 前記細胞は胚性幹細胞である、請求項6に記載の方法。
- 前記細胞は多能性細胞である、請求項1に記載の方法。
- 前記ビーズを第2のアガロース溶液でコートする前に、トランペットツールを用いて前記ビーズを前記懸濁液から取り出すことを更に含む、請求項1に記載の方法。
- トランペットツールを用いて前記ビーズを前記鉱油から取り出すことを更に含む、請求項1に記載の方法。
- 前記温度勾配は約20℃〜約50℃であり、勾配開始時に高温である、請求項1に記載の方法。
- 前記高温は約20℃〜約35℃の範囲である、請求項11に記載の方法。
- 前記高温は約20℃〜約30℃であり、前記低温は約0℃〜約−8℃である、請求項1に記載の方法。
- 前記高温は約20℃〜約25℃であり、前記低温は約0℃〜約−2℃である、請求項13に記載の方法。
- 一点から他点へ対象物を運ぶのに有用な装置であって、中空長手方向軸を有するワンピース構造物を含み、前記中空長手方向軸は、第1の端には前記中空長手方向軸と接合する逆円錐面を有し、第2の端には真空手段と接合するようになっている手段を有し、第2の端の前記手段は、直径が長手方向軸及び逆円錐手段のいずれの直径よりも大きい装置。
- 一点から他点へ対象物を運ぶのに有用な装置であって、中空長手方向軸と2個の端を有するワンピース構造物を含み、その第1の端は真空手段に接続するようになっており、第2の端は運ばれる対象物を受け入れるようになっている装置であって、流体源用の接続手段を受け入れるようになっている内部手段と、前記流体を運ぶのに用いる送達デバイスを妨げるための手段とを更に含む装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US201261592949P | 2012-01-31 | 2012-01-31 | |
US61/592,949 | 2012-01-31 | ||
PCT/US2013/023802 WO2013116306A1 (en) | 2012-01-31 | 2013-01-30 | Improved method for manufacture of macrobeads |
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JP2015510396A true JP2015510396A (ja) | 2015-04-09 |
JP5986226B2 JP5986226B2 (ja) | 2016-09-06 |
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US (2) | US9090866B2 (ja) |
EP (1) | EP2809782B1 (ja) |
JP (1) | JP5986226B2 (ja) |
KR (1) | KR101722824B1 (ja) |
CN (1) | CN103975059B (ja) |
AP (1) | AP2014007623A0 (ja) |
AU (1) | AU2013202026B2 (ja) |
BR (1) | BR112014015804B1 (ja) |
CA (1) | CA2851023C (ja) |
DK (1) | DK2809782T3 (ja) |
ES (1) | ES2647613T3 (ja) |
HK (1) | HK1199903A1 (ja) |
HU (1) | HUE034618T2 (ja) |
IL (1) | IL231834A (ja) |
MX (1) | MX350593B (ja) |
NO (1) | NO2904201T3 (ja) |
NZ (1) | NZ624195A (ja) |
PL (1) | PL2809782T3 (ja) |
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RU (1) | RU2591518C2 (ja) |
WO (1) | WO2013116306A1 (ja) |
Cited By (1)
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JP2021108658A (ja) * | 2019-12-30 | 2021-08-02 | 財團法人工業技術研究院Industrial Technology Research Institute | 細胞外小胞分離法、コロイド粒子とその調製方法 |
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EP2809782A1 (en) | 2014-12-10 |
IL231834A0 (en) | 2014-05-28 |
KR20140122742A (ko) | 2014-10-20 |
USRE47439E1 (en) | 2019-06-18 |
PL2809782T3 (pl) | 2018-04-30 |
RU2591518C2 (ru) | 2016-07-20 |
EP2809782A4 (en) | 2015-11-04 |
JP5986226B2 (ja) | 2016-09-06 |
BR112014015804A2 (pt) | 2021-08-31 |
CA2851023C (en) | 2018-03-20 |
CN103975059A (zh) | 2014-08-06 |
AU2013202026A1 (en) | 2013-08-15 |
IL231834A (en) | 2016-04-21 |
AU2013202026B2 (en) | 2014-05-08 |
RU2014133367A (ru) | 2016-03-27 |
DK2809782T3 (da) | 2017-11-20 |
MX350593B (es) | 2017-09-11 |
CN103975059B (zh) | 2016-08-31 |
WO2013116306A1 (en) | 2013-08-08 |
AP2014007623A0 (en) | 2014-05-31 |
CA2851023A1 (en) | 2013-08-08 |
US20130202802A1 (en) | 2013-08-08 |
NO2904201T3 (ja) | 2018-01-20 |
US9090866B2 (en) | 2015-07-28 |
BR112014015804B1 (pt) | 2022-10-18 |
EP2809782B1 (en) | 2017-08-23 |
KR101722824B1 (ko) | 2017-04-03 |
ES2647613T3 (es) | 2017-12-22 |
MX2014009295A (es) | 2014-09-11 |
HK1199903A1 (en) | 2015-07-24 |
NZ624195A (en) | 2015-07-31 |
HUE034618T2 (en) | 2018-02-28 |
PT2809782T (pt) | 2017-12-01 |
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