MX350593B - Metodo mejorado para la produccion de macroperlas. - Google Patents

Metodo mejorado para la produccion de macroperlas.

Info

Publication number
MX350593B
MX350593B MX2014009295A MX2014009295A MX350593B MX 350593 B MX350593 B MX 350593B MX 2014009295 A MX2014009295 A MX 2014009295A MX 2014009295 A MX2014009295 A MX 2014009295A MX 350593 B MX350593 B MX 350593B
Authority
MX
Mexico
Prior art keywords
improved method
macrobeads
manufacture
tool
beads
Prior art date
Application number
MX2014009295A
Other languages
English (en)
Other versions
MX2014009295A (es
Inventor
Smith Barry
Gazda Lawrence
Laramore Melissa
Hamilton Timothy
Original Assignee
The Rogosin Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Rogosin Inst filed Critical The Rogosin Inst
Publication of MX2014009295A publication Critical patent/MX2014009295A/es
Publication of MX350593B publication Critical patent/MX350593B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B25—HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
    • B25J—MANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
    • B25J15/00—Gripping heads and other end effectors
    • B25J15/06—Gripping heads and other end effectors with vacuum or magnetic holding means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B25—HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
    • B25J—MANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
    • B25J15/00—Gripping heads and other end effectors
    • B25J15/06—Gripping heads and other end effectors with vacuum or magnetic holding means
    • B25J15/0616—Gripping heads and other end effectors with vacuum or magnetic holding means with vacuum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/76—Agarose, agar-agar

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Mechanical Engineering (AREA)
  • Robotics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Dispersion Chemistry (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención se refiere a un método para producir una composición de material que comprende una muestra de células vivas en un glóbulo que contiene agarosa, en el cual dicho glóbulo está revestido con agarosa, que comprende: (a) mezcla runa primera muestra de agarosa con dicha muestra de células vivas para formar una suspensión; (b) mover dicha suspensión a una primera muestra de aceite mineral, para formar un glóbulo a partir de dicha suspensión; (c) retirar dicho glóbulo a partir de dicha primera muestra de aceite mineral; (d) enjuagar el aceite mineral a partir de dicho glóbulo; ( e) mover dicho glóbulo con una herramienta de trompeta a una segunda solución de agarosa; (f) revestir dicho glóbulo con dicha segunda solución de agarosa, (g) retirar dicho glóbulo a partir de dicha segunda solución de agarosa con una herramienta de popote, y (h) dispensar dicho glóbulo revestido en una segunda muestra de aceite mineral, en donde dicha muestra de aceite mineral se mantiene a un gradiente de temperatura de manera que dicho glóbulo se mueva a lo largo de una trayectoria desde una ubicación en el aceite mineral que está a una temperatura más alta de aproximadamente 20°C hasta aproximadamente 30°C hasta una ubicación en el aceite mineral a una temperatura más baja de aproximadamente 0°C hasta aproximadamente -8°C.
MX2014009295A 2012-01-31 2013-01-30 Metodo mejorado para la produccion de macroperlas. MX350593B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261592949P 2012-01-31 2012-01-31
PCT/US2013/023802 WO2013116306A1 (en) 2012-01-31 2013-01-30 Improved method for manufacture of macrobeads

Publications (2)

Publication Number Publication Date
MX2014009295A MX2014009295A (es) 2014-09-11
MX350593B true MX350593B (es) 2017-09-11

Family

ID=48903127

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014009295A MX350593B (es) 2012-01-31 2013-01-30 Metodo mejorado para la produccion de macroperlas.

Country Status (21)

Country Link
US (2) US9090866B2 (es)
EP (1) EP2809782B1 (es)
JP (1) JP5986226B2 (es)
KR (1) KR101722824B1 (es)
CN (1) CN103975059B (es)
AP (1) AP2014007623A0 (es)
AU (1) AU2013202026B2 (es)
BR (1) BR112014015804B1 (es)
CA (1) CA2851023C (es)
DK (1) DK2809782T3 (es)
ES (1) ES2647613T3 (es)
HK (1) HK1199903A1 (es)
HU (1) HUE034618T2 (es)
IL (1) IL231834A (es)
MX (1) MX350593B (es)
NO (1) NO2904201T3 (es)
NZ (1) NZ624195A (es)
PL (1) PL2809782T3 (es)
PT (1) PT2809782T (es)
RU (1) RU2591518C2 (es)
WO (1) WO2013116306A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017020141A1 (es) * 2015-08-03 2017-02-09 Vera Campos Claudio Herramienta, sistema y procedimiento automatizado de toma de muestras
KR20180117179A (ko) * 2016-03-01 2018-10-26 옥스포드 유니버시티 이노베이션 리미티드 셀룰러화 스캐폴드의 3d 인쇄
DE102017106867B4 (de) * 2017-03-30 2021-12-02 Hamilton Bonaduz Ag Verfahren zur Herstellung einer Portionseinheit
US20210197162A1 (en) * 2019-12-30 2021-07-01 Industrial Technology Research Institute Extracellular vesicle separation method, colloidal particle and preparation method thereof
TW202208062A (zh) * 2020-08-20 2022-03-01 財桂生物股份有限公司 微量管上蓋及其組裝裝置

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1418228E (pt) * 1994-01-13 2006-09-29 Rogosin Inst Processo de preparacao de uma biblioteca multicombinatoria de vectores de expressao de genes de anticorpos.
US6303151B1 (en) 1996-04-03 2001-10-16 The Rogosin Institute Cancer-cell proliferation-suppressing material produced by cancer cells restricted by entrapment
DE69725317T2 (de) * 1996-04-03 2004-07-29 The Rogosin Institute Implantierbare agarose-kollagenkügelchen enthaltende zellen, die ein diffusionsfähiges biologisches produkt bilden und ihre verwendung
US6224912B1 (en) 1996-04-03 2001-05-01 The Rogo Institute Cancer-cell proliferation-suppressing material produced by cancer cells restricted by entrapment
US5888497A (en) 1996-04-03 1999-03-30 The Rogosin Institute Agarose coated agarose beads containing cancer cells that produce material which suppresses cancer cell proliferation
US20030012699A1 (en) * 1998-11-18 2003-01-16 Thomas Moore Simultaneous handling of magnetic beads in a two-dimensional arrangement
US6497155B1 (en) 1999-02-09 2002-12-24 Pharmacopeia, Inc. Article comprising a particle retrieval device
US6558665B1 (en) 1999-05-18 2003-05-06 Arch Development Corporation Encapsulating particles with coatings that conform to size and shape of the particles
JP2001168127A (ja) 1999-12-08 2001-06-22 Japan Em Kk 微小球体移載装置
US20050053586A1 (en) 2003-09-04 2005-03-10 Bryan Conn Entrapped stem cells and uses thereof
JP2005114576A (ja) 2003-10-08 2005-04-28 Hitachi Software Eng Co Ltd 両親媒性分子固定化ビーズ、その製造方法、及びキャピラリビーズアレイのビーズ配列方法
US8359971B2 (en) * 2004-02-25 2013-01-29 Andhow Innovations, Llc Leak resistant siphoning device for use in fluid transfer
JP4520359B2 (ja) 2005-05-13 2010-08-04 日立ソフトウエアエンジニアリング株式会社 粒子捕捉装置、並びに粒子配列方法及び粒子配列装置
SG131015A1 (en) * 2005-09-15 2007-04-26 Millipore Corp Method and apparatus for making porous agarose beads
CA2622529C (en) * 2005-09-26 2014-02-18 Lawrence Gazda Secretory cell-containing macrobeads comprising seakem gold agarose, and uses thereof
JP5658566B2 (ja) 2007-09-29 2015-01-28 イーアイ・スペクトラ・エルエルシー 計装ピペット先端
EP2435490A1 (en) * 2009-05-28 2012-04-04 Corning Incorporated Swellable synthetic microcarriers for culturing cells
USD625429S1 (en) 2009-10-01 2010-10-12 Holger Link Pipette
DE102010040685A1 (de) 2010-09-14 2012-03-15 Hamilton Bonaduz Ag Temperiervorrichtung zur thermischen Verfestigung von Wirkstoff-Beads
DE102010040687A1 (de) * 2010-09-14 2012-03-15 Hamilton Bonaduz Ag Verfahren zum Herstellen von Wirkstoff-Beads
DE102010040681A1 (de) 2010-09-14 2012-03-15 Hamilton Bonaduz Ag Unterdruckgreifer zur Handhabung von Wirkstoff-Beads mit vorzugsweise konkaver Bead-Kontaktfläche
DE102010040684A1 (de) 2010-09-14 2012-03-15 Hamilton Bonaduz Ag Röhrenförmiger Unterdruckgreifer zur Handhabung von Rohlingen von Wirkstoff-Beads
DE102011050024A1 (de) 2011-04-29 2012-10-31 Hamilton Bonaduz Ag Analysevorrichtung für eine berührungslose Analyse der Ausformung eines transparenten Körpers und Verfahren zur Durchführung der berührungslosen Analyse

Also Published As

Publication number Publication date
EP2809782A1 (en) 2014-12-10
IL231834A0 (en) 2014-05-28
KR20140122742A (ko) 2014-10-20
USRE47439E1 (en) 2019-06-18
PL2809782T3 (pl) 2018-04-30
RU2591518C2 (ru) 2016-07-20
EP2809782A4 (en) 2015-11-04
JP5986226B2 (ja) 2016-09-06
BR112014015804A2 (pt) 2021-08-31
CA2851023C (en) 2018-03-20
CN103975059A (zh) 2014-08-06
AU2013202026A1 (en) 2013-08-15
IL231834A (en) 2016-04-21
AU2013202026B2 (en) 2014-05-08
RU2014133367A (ru) 2016-03-27
DK2809782T3 (da) 2017-11-20
JP2015510396A (ja) 2015-04-09
CN103975059B (zh) 2016-08-31
WO2013116306A1 (en) 2013-08-08
AP2014007623A0 (en) 2014-05-31
CA2851023A1 (en) 2013-08-08
US20130202802A1 (en) 2013-08-08
NO2904201T3 (es) 2018-01-20
US9090866B2 (en) 2015-07-28
BR112014015804B1 (pt) 2022-10-18
EP2809782B1 (en) 2017-08-23
KR101722824B1 (ko) 2017-04-03
ES2647613T3 (es) 2017-12-22
MX2014009295A (es) 2014-09-11
HK1199903A1 (en) 2015-07-24
NZ624195A (en) 2015-07-31
HUE034618T2 (en) 2018-02-28
PT2809782T (pt) 2017-12-01

Similar Documents

Publication Publication Date Title
WO2011131746A3 (en) Heterodimeric antibody fc-containing proteins and methods for production thereof
PL2986735T3 (pl) Sposób określania składu ilościowego komórek krwi w próbce
HK1218297A1 (zh) 用於生產單克隆抗體的改進方法
EP2770090A4 (en) METHOD FOR PRODUCING A NUCLEIC ACID LIBRARY AND USES THEREOF AND KITS THEREFOR
MX350593B (es) Metodo mejorado para la produccion de macroperlas.
MX2015011781A (es) Medio de cultivo celular y metodos de produccion de anticuerpos.
EP2782023A4 (en) METHOD FOR THE AUTOMATED ANALYSIS OF TEXT DOCUMENTS
EP3362784A4 (en) SYSTEM AND METHOD FOR REGULATING THE CELL CULTURE BASED MANUFACTURE OF BIOLOGICS
GB2515626B (en) PCBN Material, Method for making same, tools comprising same and method of using same
MX2019012139A (es) Preparacion de 18f-fluciclovina.
PT2737060T (pt) Método para a produção de partículas retrovirais úteis para transdução de células eucarióticas
HK1225798B (zh) 試劑用於裂解紅細胞的用途以及涉及其的方法和試劑盒
HUE055177T2 (hu) Brikett kõzetgyapot elõállításához, és eljárás a szóban forgó brikett elõállítására
WO2012001073A3 (en) Improved method for selecting high producing cell lines
WO2012112958A3 (en) High-throughput assays to probe leukemia within the stromal niche
EP2811281A4 (en) COMPOSITION FOR ENHANCING A BIOLOGICAL SAMPLE, PROCESS FOR PREPARING A PARAFFIN BLOCK USING THE SAME, AND PROCESS FOR PREPARING A CELL BLOCK TEST FROM THE PARAFFIN BLOCK TO FORM A CELL MICROMATOR
WO2012113906A3 (en) Solid support and method of enhancing the recovery of biological material therefrom
PL3080286T3 (pl) Sposób wytwarzania oleju mikrobiologicznego z materiałów lignocelulozowych
EP2918682A4 (en) METHOD USING STRAIN OF HIGH-DENSITY CULTIVATED FUNGAL FUNGI FOR MANUFACTURING USEFUL MATERIAL
EP3020805A4 (en) CULTURE MANUFACTURING METHOD COMPRISING VIRAL PSEUDO PARTICLES
DK2727979T3 (da) Fischer-tropsch-fremgangsmåde til fremstilling af carbonhydrider på grundlag af biogas
EP3041814B8 (en) Process for the production of dichlorohydrins
CL2016000874A1 (es) Anticuerpos anti-cd38 (divisional de sol. 1944-13).
FR2972065B1 (fr) Procede d’attribution d’identifiants a des objets, et de discrimination desdits objets a partir des identifiants attribues
UA61819U (ru) Способ диагностики состояния организма человека

Legal Events

Date Code Title Description
FG Grant or registration