JP2014526262A5 - - Google Patents
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- Publication number
- JP2014526262A5 JP2014526262A5 JP2014530656A JP2014530656A JP2014526262A5 JP 2014526262 A5 JP2014526262 A5 JP 2014526262A5 JP 2014530656 A JP2014530656 A JP 2014530656A JP 2014530656 A JP2014530656 A JP 2014530656A JP 2014526262 A5 JP2014526262 A5 JP 2014526262A5
- Authority
- JP
- Japan
- Prior art keywords
- activity
- fatty acid
- polypeptide
- microbial cell
- recombinant microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000813 microbial effect Effects 0.000 claims description 102
- 230000000694 effects Effects 0.000 claims description 98
- 229920001184 polypeptide Polymers 0.000 claims description 58
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 58
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 58
- 239000002157 polynucleotide Substances 0.000 claims description 50
- 102000040430 polynucleotide Human genes 0.000 claims description 50
- 108091033319 polynucleotide Proteins 0.000 claims description 50
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 49
- 229930195729 fatty acid Natural products 0.000 claims description 49
- 239000000194 fatty acid Substances 0.000 claims description 49
- 150000004665 fatty acids Chemical class 0.000 claims description 49
- 229940053200 antiepileptics fatty acid derivative Drugs 0.000 claims description 27
- QAQREVBBADEHPA-IEXPHMLFSA-N propionyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QAQREVBBADEHPA-IEXPHMLFSA-N 0.000 claims description 23
- 102000004190 Enzymes Human genes 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 15
- 230000014509 gene expression Effects 0.000 claims description 14
- 150000002191 fatty alcohols Chemical class 0.000 claims description 12
- 150000002192 fatty aldehydes Chemical class 0.000 claims description 12
- 108010019608 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase Proteins 0.000 claims description 11
- 102100037149 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial Human genes 0.000 claims description 11
- 150000002194 fatty esters Chemical class 0.000 claims description 10
- 229930195733 hydrocarbon Natural products 0.000 claims description 10
- 150000002430 hydrocarbons Chemical class 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 108010064711 Homoserine dehydrogenase Proteins 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 230000001851 biosynthetic effect Effects 0.000 claims description 6
- 150000001336 alkenes Chemical class 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 108010028984 3-isopropylmalate dehydratase Proteins 0.000 claims description 4
- 108010039636 3-isopropylmalate dehydrogenase Proteins 0.000 claims description 4
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 4
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims description 4
- 108010055400 Aspartate kinase Proteins 0.000 claims description 4
- 239000004215 Carbon black (E152) Substances 0.000 claims description 4
- 102100029106 Ethylmalonyl-CoA decarboxylase Human genes 0.000 claims description 4
- 108010085747 Methylmalonyl-CoA Decarboxylase Proteins 0.000 claims description 4
- 108010051679 Methylmalonyl-CoA carboxytransferase Proteins 0.000 claims description 4
- 102000005488 Thioesterase Human genes 0.000 claims description 4
- 108010006873 Threonine Dehydratase Proteins 0.000 claims description 4
- 102000006843 Threonine synthase Human genes 0.000 claims description 4
- 108010022394 Threonine synthase Proteins 0.000 claims description 4
- 102100033451 Thyroid hormone receptor beta Human genes 0.000 claims description 4
- 230000002238 attenuated effect Effects 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 150000002148 esters Chemical class 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 108010071598 homoserine kinase Proteins 0.000 claims description 4
- 108020002982 thioesterase Proteins 0.000 claims description 4
- 108010063585 (R)-citramalate synthase Proteins 0.000 claims description 3
- 102000019010 Methylmalonyl-CoA Mutase Human genes 0.000 claims description 3
- 108010051862 Methylmalonyl-CoA mutase Proteins 0.000 claims description 3
- 108010082340 Arginine deiminase Proteins 0.000 claims description 2
- 108090000489 Carboxy-Lyases Proteins 0.000 claims description 2
- 102000004031 Carboxy-Lyases Human genes 0.000 claims description 2
- 108030002325 Carboxylate reductases Proteins 0.000 claims description 2
- 101710129019 Long-chain acyl-[acyl-carrier-protein] reductase Proteins 0.000 claims description 2
- 102000030503 Methylmalonyl-CoA epimerase Human genes 0.000 claims description 2
- 241000795633 Olea <sea slug> Species 0.000 claims description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 108090001018 hexadecanal dehydrogenase (acylating) Proteins 0.000 claims description 2
- 150000002576 ketones Chemical class 0.000 claims description 2
- 108091000124 methylmalonyl-CoA epimerase Proteins 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- 230000002018 overexpression Effects 0.000 claims description 2
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 claims 2
- 239000013256 coordination polymer Substances 0.000 claims 1
- XFTRTWQBIOMVPK-UHFFFAOYSA-N (-)-(R)-2-hydroxy-2-methylbutanedioic acid Natural products OC(=O)C(O)(C)CC(O)=O XFTRTWQBIOMVPK-UHFFFAOYSA-N 0.000 description 1
- GGQQNYXPYWCUHG-RMTFUQJTSA-N (3e,6e)-deca-3,6-diene Chemical compound CCC\C=C\C\C=C\CC GGQQNYXPYWCUHG-RMTFUQJTSA-N 0.000 description 1
- XFTRTWQBIOMVPK-RXMQYKEDSA-N D-citramalic acid Chemical compound OC(=O)[C@@](O)(C)CC(O)=O XFTRTWQBIOMVPK-RXMQYKEDSA-N 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38308610P | 2010-09-15 | 2010-09-15 | |
| US13/232,927 | 2011-09-14 | ||
| US13/232,927 US8372610B2 (en) | 2010-09-15 | 2011-09-14 | Production of odd chain fatty acid derivatives in recombinant microbial cells |
| PCT/US2012/028256 WO2013039563A1 (en) | 2010-09-15 | 2012-03-08 | Production of odd chain fatty acid derivatives in recombinant microbial cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017011738A Division JP6609274B2 (ja) | 2011-09-14 | 2017-01-26 | 組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014526262A JP2014526262A (ja) | 2014-10-06 |
| JP2014526262A5 true JP2014526262A5 (https=) | 2015-03-12 |
| JP6141282B2 JP6141282B2 (ja) | 2017-06-07 |
Family
ID=47884064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014530656A Expired - Fee Related JP6141282B2 (ja) | 2010-09-15 | 2012-03-08 | 組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US8372610B2 (https=) |
| EP (2) | EP3957736B1 (https=) |
| JP (1) | JP6141282B2 (https=) |
| CN (1) | CN103906845B (https=) |
| CA (1) | CA2848253C (https=) |
| MY (1) | MY191370A (https=) |
| WO (1) | WO2013039563A1 (https=) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3101888A1 (en) | 2008-05-16 | 2009-11-19 | REG Life Sciences, LLC | Methods and compositions for producing hydrocarbons |
| KR101636403B1 (ko) | 2008-10-28 | 2016-07-05 | 알이지 라이프 사이언시스, 엘엘씨 | 지방족 알코올들을 생산하기 위한 방법들과 조성물들 |
| WO2013148348A1 (en) * | 2012-03-28 | 2013-10-03 | Kiverdi, Inc. | Engineered co2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same |
| US9957534B2 (en) | 2008-11-06 | 2018-05-01 | Kiverdi, Inc. | Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same |
| MX2012003604A (es) | 2009-09-27 | 2012-09-12 | Opx Biotechnologies Inc | Metodo para producir acido 3-hidroxipropionico y otros productos. |
| EP2834352B1 (en) * | 2012-04-02 | 2020-01-08 | Genomatica, Inc. | Improved production of fatty acid derivatives |
| MX2015001780A (es) | 2012-08-10 | 2015-09-28 | Opx Biotechnologies Inc | Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos. |
| EP2931742A2 (en) | 2012-12-12 | 2015-10-21 | REG Life Sciences, LLC | Acp-mediated production of fatty acid derivatives |
| US20140193867A1 (en) * | 2012-12-21 | 2014-07-10 | William Marsh Rice University | Microbial odd chain fatty acids |
| JP6063585B2 (ja) | 2013-01-16 | 2017-01-18 | アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー | 改良された特性を有するアシル−ａｃｐレダクターゼ |
| CA2902279C (en) | 2013-03-05 | 2019-05-28 | The Procter & Gamble Company | Mixed sugar amine or sugar amide surfactant compositions |
| US20150119601A1 (en) | 2013-03-15 | 2015-04-30 | Opx Biotechnologies, Inc. | Monofunctional mcr + 3-hp dehydrogenase |
| SI2984490T1 (sl) | 2013-06-14 | 2020-10-30 | Genomatica, Inc. | Metode proizvodnje derivatov omega hidroksilirane maščobne kisline |
| EP3022310B1 (en) * | 2013-07-19 | 2019-10-16 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| US11408013B2 (en) | 2013-07-19 | 2022-08-09 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| US9469586B2 (en) | 2013-08-19 | 2016-10-18 | REG Life Sciences, LLC | Production of partially refined waste glycerol |
| KR20190017074A (ko) | 2013-08-19 | 2019-02-19 | 알이지 라이프 사이언시스, 엘엘씨 | 부분 정제된 폐글리세롤의 생성 |
| AU2014318531B2 (en) | 2013-09-13 | 2020-10-08 | Genomatica, Inc. | Improved acetyl-CoA carboxylase variants |
| US20160376600A1 (en) | 2013-11-25 | 2016-12-29 | Genomatica, Inc. | Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol |
| WO2015085271A1 (en) | 2013-12-05 | 2015-06-11 | REG Life Sciences, LLC | Microbial production of fatty amines |
| WO2015089127A1 (en) * | 2013-12-12 | 2015-06-18 | Dow Global Technologies Llc | Processes to prepare elongated 2-ketoacids and c6-c10 compounds therefrom via genetic modifications to microbial metabolic pathways |
| EP3129492B1 (en) * | 2014-04-10 | 2020-06-03 | Genomatica, Inc. | Chemo-enzymatic process for the preparation of lactones and macrocyclic ketones |
| CN106604990B (zh) | 2014-06-16 | 2022-04-05 | 基因组股份公司 | 具有改进的特性的ω-羟化酶相关的融合多肽 |
| EP3155111A1 (en) * | 2014-06-16 | 2017-04-19 | Invista Technologies S.à r.l. | Process for producing glutarate and glutaric acid methyl ester |
| EP4089176A1 (en) | 2014-07-18 | 2022-11-16 | Genomatica, Inc. | Microbial production of 1,3 diols |
| EP2993228B1 (en) | 2014-09-02 | 2019-10-09 | Cargill, Incorporated | Production of fatty acid esters |
| US10260060B2 (en) | 2014-12-10 | 2019-04-16 | Dow Global Technologies Llc | Genetically modified phenylpyruvate decarboxylase, processes to prepare, and uses thereof |
| US10851393B2 (en) * | 2015-02-24 | 2020-12-01 | William Marsh Rice University | KAS-III free FA synthesis |
| JP6681625B2 (ja) * | 2015-06-16 | 2020-04-15 | 国立大学法人名古屋大学 | タンパク質の発現方法 |
| WO2017101987A1 (en) | 2015-12-15 | 2017-06-22 | REG Life Sciences, LLC | Omega-hydroxylase-related fusion polypeptide variants with improved properties |
| CN106916856B (zh) * | 2015-12-28 | 2021-08-24 | 丰益(上海)生物技术研发中心有限公司 | 提高产脂微生物生产奇数碳脂肪酸产量的培养基及方法 |
| EP3512825B1 (en) | 2016-09-14 | 2020-10-21 | Genomatica, Inc. | 1,3-fatty diol compounds and derivatives thereof |
| EP3519579B1 (en) | 2016-09-30 | 2025-11-05 | Dow Global Technologies LLC | Processes to prepare elongated 2-ketoacids and c-5-c10 compounds therefrom via genetic modifications to microbial metabolic pathways |
| US11345938B2 (en) | 2017-02-02 | 2022-05-31 | Cargill, Incorporated | Genetically modified cells that produce C6-C10 fatty acid derivatives |
| MY205555A (en) | 2017-04-03 | 2024-10-25 | Genomatica Inc | Thioesterase variants having improved activity for the production of medium-chain fatty acid derivatives |
| WO2019056071A1 (en) * | 2017-09-21 | 2019-03-28 | The University Of Queensland | RECOMBINANT MICROORGANISMS |
| US11230705B2 (en) | 2017-09-29 | 2022-01-25 | Dow Global Technologies Llc | Genetically modified isopropylmalate isomerase enzyme complexes and processes to prepare elongated 2-ketoacids and C5-C10 compounds therewith |
| EP3844179A4 (en) | 2018-08-31 | 2022-08-31 | Genomatica, Inc. | XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE |
| JP7601774B2 (ja) * | 2018-10-04 | 2024-12-17 | アンナ・ユニバーシティ | シトラマル酸、シトラコン酸又は２－オキソ酪酸からのｌ－２－アミノ酪酸の生成 |
| CN109517811B (zh) * | 2018-11-29 | 2022-11-11 | 滨州医学院 | 一种β-酮脂酰-ACP合成酶突变体 |
| CN110129400B (zh) * | 2019-05-25 | 2023-09-01 | 华南理工大学 | 一种提高微生物油脂奇数碳链脂肪酸含量的方法 |
| CN112941096B (zh) * | 2019-12-10 | 2023-05-26 | 北京化工大学 | 重组质粒组合、基因改造酵母菌及生产奇数链脂肪酸方法 |
| WO2023178197A1 (en) | 2022-03-16 | 2023-09-21 | Genomatica, Inc. | Recombinant microbes for production of trans-2 unsaturated fatty acids and derivatives thereof |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991016427A1 (en) | 1990-04-24 | 1991-10-31 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US6428767B1 (en) | 1995-05-12 | 2002-08-06 | E. I. Du Pont De Nemours And Company | Method for identifying the source of carbon in 1,3-propanediol |
| CA2650773C (en) | 2006-05-19 | 2014-12-02 | Jay D. Keasling | Microorganisms transformed with acetyl-coa carboxylase and wax synthase for production of fatty acid derivatives |
| CN101490241A (zh) * | 2006-05-19 | 2009-07-22 | Ls9公司 | 脂肪酸及其衍生物的制备 |
| US8110670B2 (en) * | 2006-05-19 | 2012-02-07 | Ls9, Inc. | Enhanced production of fatty acid derivatives |
| PL389665A1 (pl) * | 2007-02-09 | 2010-07-05 | The Regents of the University of CaliforniaThe Regents of the University of California | Wytwarzanie biopaliwa przez rekombinowane mikroorganizmy |
| US9695426B2 (en) | 2007-02-09 | 2017-07-04 | The Regents Of The University Of California | Biofuel production by recombinant microorganisms |
| CN101680009B (zh) | 2007-03-28 | 2017-02-22 | Reg生命科学有限责任公司 | 提高脂肪酸衍生物的产量 |
| BRPI0813359A2 (pt) | 2007-05-22 | 2014-10-14 | Ls9 Inc | Ácidos nucleicos isolados, vetor, células, métodos para produzir um hidrocarboneto, para produzir uma cetona alifática, para produzir um polipeptídeo purificado, para produzir um hidrocarboneto, para produzir biocombustíveis, hidrocarboneto, biocombustível, polipeptídeo isolado, ácido nucleico isolado, organismos geneticamente engenheirados, método para identificar uma enzima útil para a produção de hidrocarbonetos |
| EP2615164A1 (en) * | 2007-11-10 | 2013-07-17 | Joule Unlimited Technologies, Inc. | Hyperphotosynthetic organisms |
| WO2009085278A1 (en) | 2007-12-21 | 2009-07-09 | Ls9, Inc. | Methods and compositions for producing olefins |
| CA3101888A1 (en) | 2008-05-16 | 2009-11-19 | REG Life Sciences, LLC | Methods and compositions for producing hydrocarbons |
| KR101717569B1 (ko) | 2008-10-07 | 2017-03-17 | 알이지 라이프 사이언시스, 엘엘씨 | 지방족 알데히드를 생산하기 위한 방법과 조성물들 |
| KR101636403B1 (ko) | 2008-10-28 | 2016-07-05 | 알이지 라이프 사이언시스, 엘엘씨 | 지방족 알코올들을 생산하기 위한 방법들과 조성물들 |
| CA3055144C (en) | 2008-12-23 | 2022-07-19 | REG Life Sciences, LLC | Methods and compositions related to thioesterase enzymes |
| BRPI1010920B1 (pt) * | 2009-05-22 | 2020-04-28 | Codexis Inc | processo para a produção biológica de álcoois graxos |
| US8715971B2 (en) | 2009-09-09 | 2014-05-06 | Genomatica, Inc. | Microorganisms and methods for the co-production of isopropanol and 1,4-butanediol |
| US20110166370A1 (en) | 2010-01-12 | 2011-07-07 | Charles Winston Saunders | Scattered Branched-Chain Fatty Acids And Biological Production Thereof |
-
2011
- 2011-09-14 US US13/232,927 patent/US8372610B2/en not_active Expired - Fee Related
-
2012
- 2012-03-08 MY MYPI2014000550A patent/MY191370A/en unknown
- 2012-03-08 JP JP2014530656A patent/JP6141282B2/ja not_active Expired - Fee Related
- 2012-03-08 WO PCT/US2012/028256 patent/WO2013039563A1/en not_active Ceased
- 2012-03-08 CA CA2848253A patent/CA2848253C/en active Active
- 2012-03-08 US US14/344,658 patent/US20150167033A1/en not_active Abandoned
- 2012-03-08 CN CN201280054084.6A patent/CN103906845B/zh active Active
- 2012-03-08 EP EP21177335.3A patent/EP3957736B1/en active Active
- 2012-03-08 EP EP12709480.3A patent/EP2756090B1/en active Active
-
2021
- 2021-02-08 US US17/169,845 patent/US20210324431A1/en active Pending
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