JP2014520551A5 - - Google Patents
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- JP2014520551A5 JP2014520551A5 JP2014520269A JP2014520269A JP2014520551A5 JP 2014520551 A5 JP2014520551 A5 JP 2014520551A5 JP 2014520269 A JP2014520269 A JP 2014520269A JP 2014520269 A JP2014520269 A JP 2014520269A JP 2014520551 A5 JP2014520551 A5 JP 2014520551A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- nucleic acid
- cell
- culturing
- acid molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 210000004027 cell Anatomy 0.000 claims description 87
- 238000000034 method Methods 0.000 claims description 61
- 150000007523 nucleic acids Chemical class 0.000 claims description 57
- 108020004707 nucleic acids Proteins 0.000 claims description 55
- 102000039446 nucleic acids Human genes 0.000 claims description 55
- 238000012258 culturing Methods 0.000 claims description 25
- 230000008672 reprogramming Effects 0.000 claims description 25
- 239000002609 medium Substances 0.000 claims description 22
- 210000001082 somatic cell Anatomy 0.000 claims description 13
- 239000003112 inhibitor Substances 0.000 claims description 12
- 230000002068 genetic effect Effects 0.000 claims description 10
- 239000013598 vector Substances 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 5
- 230000010354 integration Effects 0.000 claims description 5
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims description 4
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims description 4
- 102000019058 Glycogen Synthase Kinase 3 beta Human genes 0.000 claims description 4
- 108010051975 Glycogen Synthase Kinase 3 beta Proteins 0.000 claims description 4
- 102000004058 Leukemia inhibitory factor Human genes 0.000 claims description 4
- 108090000581 Leukemia inhibitory factor Proteins 0.000 claims description 4
- 229940124647 MEK inhibitor Drugs 0.000 claims description 4
- 108091005735 TGF-beta receptors Proteins 0.000 claims description 4
- 108091023040 Transcription factor Proteins 0.000 claims description 4
- 102000040945 Transcription factor Human genes 0.000 claims description 4
- 102000016715 Transforming Growth Factor beta Receptors Human genes 0.000 claims description 4
- 238000010195 expression analysis Methods 0.000 claims description 4
- 229940126864 fibroblast growth factor Drugs 0.000 claims description 4
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims description 4
- 238000002826 magnetic-activated cell sorting Methods 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims description 4
- 239000002829 mitogen activated protein kinase inhibitor Substances 0.000 claims description 4
- 239000013603 viral vector Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 3
- 108091026890 Coding region Proteins 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 claims description 2
- 241000702421 Dependoparvovirus Species 0.000 claims description 2
- 102000004533 Endonucleases Human genes 0.000 claims description 2
- 108010042407 Endonucleases Proteins 0.000 claims description 2
- 101001000998 Homo sapiens Protein phosphatase 1 regulatory subunit 12C Proteins 0.000 claims description 2
- 108700021430 Kruppel-Like Factor 4 Proteins 0.000 claims description 2
- 102000005640 Myosin Type II Human genes 0.000 claims description 2
- 108010045128 Myosin Type II Proteins 0.000 claims description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- 108091000080 Phosphotransferase Proteins 0.000 claims description 2
- 102100035620 Protein phosphatase 1 regulatory subunit 12C Human genes 0.000 claims description 2
- 206010043276 Teratoma Diseases 0.000 claims description 2
- 210000001789 adipocyte Anatomy 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 claims description 2
- 210000001771 cumulus cell Anatomy 0.000 claims description 2
- 230000002950 deficient Effects 0.000 claims description 2
- 230000004069 differentiation Effects 0.000 claims description 2
- 239000012636 effector Substances 0.000 claims description 2
- 210000002889 endothelial cell Anatomy 0.000 claims description 2
- 230000001973 epigenetic effect Effects 0.000 claims description 2
- 210000002919 epithelial cell Anatomy 0.000 claims description 2
- 210000002950 fibroblast Anatomy 0.000 claims description 2
- 238000000684 flow cytometry Methods 0.000 claims description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 2
- 230000009395 genetic defect Effects 0.000 claims description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 2
- 230000006801 homologous recombination Effects 0.000 claims description 2
- 238000002744 homologous recombination Methods 0.000 claims description 2
- 230000005847 immunogenicity Effects 0.000 claims description 2
- 208000015181 infectious disease Diseases 0.000 claims description 2
- 210000004153 islets of langerhan Anatomy 0.000 claims description 2
- 210000002510 keratinocyte Anatomy 0.000 claims description 2
- 210000003292 kidney cell Anatomy 0.000 claims description 2
- 101150111214 lin-28 gene Proteins 0.000 claims description 2
- 210000005229 liver cell Anatomy 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 210000000663 muscle cell Anatomy 0.000 claims description 2
- 210000002569 neuron Anatomy 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 244000052769 pathogen Species 0.000 claims description 2
- 230000001717 pathogenic effect Effects 0.000 claims description 2
- 102000020233 phosphotransferase Human genes 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 230000010076 replication Effects 0.000 claims description 2
- 239000011435 rock Substances 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 claims description 2
- 210000004927 skin cell Anatomy 0.000 claims description 2
- 230000002103 transcriptional effect Effects 0.000 claims description 2
- 230000002463 transducing effect Effects 0.000 claims description 2
- 230000009466 transformation Effects 0.000 claims description 2
- 241001430294 unidentified retrovirus Species 0.000 claims description 2
- 230000000762 glandular Effects 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161506314P | 2011-07-11 | 2011-07-11 | |
| US61/506,314 | 2011-07-11 | ||
| PCT/US2012/046194 WO2013009825A1 (en) | 2011-07-11 | 2012-07-11 | Methods for cell reprogramming and genome engineering |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014520551A JP2014520551A (ja) | 2014-08-25 |
| JP2014520551A5 true JP2014520551A5 (enExample) | 2015-08-13 |
Family
ID=46516887
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014520269A Pending JP2014520551A (ja) | 2011-07-11 | 2012-07-11 | 細胞のリプログラミング方法およびゲノムの改変方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20130040302A1 (enExample) |
| EP (1) | EP2732029B1 (enExample) |
| JP (1) | JP2014520551A (enExample) |
| CA (1) | CA2841165A1 (enExample) |
| WO (1) | WO2013009825A1 (enExample) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3260140T3 (da) | 2011-12-05 | 2021-04-19 | Factor Bioscience Inc | Fremgangsmåder og produkter til transficering af celler |
| US8497124B2 (en) | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
| BR122019025681B1 (pt) | 2012-11-01 | 2023-04-18 | Factor Bioscience Inc | Método para inserir uma sequência de ácido nucleico em uma localização segura de um genoma de uma célula |
| BR112015022848B1 (pt) | 2013-03-15 | 2022-10-11 | Cibus Europe B.V. | Método para introdução de uma mutação mediada por gron em uma sequência de dna alvo em um protoplasto vegetal e uso de uma ou mais endonucleases sítio específica que introduzem cortes de fita única |
| EP2792742A1 (en) | 2013-04-17 | 2014-10-22 | Universitätsklinikum Hamburg-Eppendorf (UKE) | Gene-therapy vectors for treating cardiomyopathy |
| WO2015006725A2 (en) * | 2013-07-12 | 2015-01-15 | Cedars-Sinai Medical Center | Generation of induced pluripotent stem cells from normal human mammary epithelial cells |
| KR101655383B1 (ko) * | 2013-07-27 | 2016-09-08 | 고려대학교 산학협력단 | 소분자 화합물을 포함하는 만능성 줄기세포의 염색체 안정성 유지용 조성물 |
| WO2015117021A1 (en) * | 2014-01-31 | 2015-08-06 | Factor Bioscience Inc. | Methods and products for nucleic acid production and delivery |
| CN105039399A (zh) * | 2014-04-23 | 2015-11-11 | 复旦大学 | 多能干细胞-遗传性心肌病心肌细胞及其制备方法 |
| US10626372B1 (en) | 2015-01-26 | 2020-04-21 | Fate Therapeutics, Inc. | Methods and compositions for inducing hematopoietic cell differentiation |
| EP3543339A1 (en) | 2015-02-13 | 2019-09-25 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
| KR20250141836A (ko) | 2015-11-04 | 2025-09-29 | 페이트 세러퓨틱스, 인코포레이티드 | 만능 세포의 유전자 조작 |
| CN108473961B (zh) | 2015-11-04 | 2022-11-29 | 菲特治疗公司 | 用于诱导造血细胞分化的方法和组合物 |
| CA3010764A1 (en) * | 2016-01-12 | 2017-07-20 | Lonza Walkersville, Inc. | Methods and vectors to produce vector free induced pluripotent stem cells |
| WO2017196175A1 (en) | 2016-05-12 | 2017-11-16 | Erasmus University Medical Center Rotterdam | A method for culturing myogenic cells, cultures obtained therefrom, screening methods, and cell culture medium. |
| US10221395B2 (en) | 2016-06-16 | 2019-03-05 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
| US11572545B2 (en) | 2016-06-16 | 2023-02-07 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
| CN109803977B (zh) | 2016-08-17 | 2023-03-17 | 菲克特生物科学股份有限公司 | 核酸产品及其施用方法 |
| CN110637090A (zh) * | 2016-11-02 | 2019-12-31 | D·基维利希 | 用于表达大型核酸转基因的质粒载体 |
| MX2019011897A (es) * | 2017-04-18 | 2019-11-28 | Fujifilm Cellular Dynamics Inc | Celulas efectoras inmunitarias especificas de antigenos. |
| NL2019517B1 (en) | 2017-09-08 | 2019-03-19 | Univ Erasmus Med Ct Rotterdam | New therapy for Pompe disease |
| JP7344877B2 (ja) | 2017-12-28 | 2023-09-14 | ザ ジェイ． デビッド グラッドストーン インスティテューツ、 ア テスタメンタリー トラスト エスタブリッシュド アンダー ザ ウィル オブ ジェイ． デビッド グラッドストーン | Ｃｒｉｓｐｒ活性化による人工多能性細胞の生成 |
| JP7473209B2 (ja) | 2018-01-29 | 2024-04-23 | 中国科学院動物研究所 | 細胞誘導の方法 |
| CN110016483A (zh) * | 2019-04-18 | 2019-07-16 | 安徽农业大学 | 一种研究卵母细胞体外成熟中卵丘细胞基因功能的方法 |
| WO2021003462A1 (en) | 2019-07-03 | 2021-01-07 | Factor Bioscience Inc. | Cationic lipids and uses thereof |
| US10501404B1 (en) | 2019-07-30 | 2019-12-10 | Factor Bioscience Inc. | Cationic lipids and transfection methods |
| MY209860A (en) | 2020-02-13 | 2025-08-07 | Tenaya Therapeutics Inc | Gene therapy vectors for treating heart disease |
| EP3922431A1 (en) | 2020-06-08 | 2021-12-15 | Erasmus University Medical Center Rotterdam | Method of manufacturing microdevices for lab-on-chip applications |
| CN113201481B (zh) * | 2021-04-19 | 2023-10-13 | 清华大学深圳国际研究生院 | 皮肤微球及其制备方法和应用 |
| GB202115676D0 (en) * | 2021-11-01 | 2021-12-15 | Icahn School Med Mount Sinai | Methods and compositions for bat ipsc preparation and use |
| WO2024091801A2 (en) * | 2022-10-13 | 2024-05-02 | President And Fellows Of Harvard College | Methods and compositions for inducing cell differentiation |
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| EP3450545B1 (en) * | 2008-10-24 | 2023-08-23 | Wisconsin Alumni Research Foundation | Pluripotent stem cells obtained by non-viral reprogramming |
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-
2012
- 2012-07-11 JP JP2014520269A patent/JP2014520551A/ja active Pending
- 2012-07-11 WO PCT/US2012/046194 patent/WO2013009825A1/en not_active Ceased
- 2012-07-11 US US13/546,365 patent/US20130040302A1/en not_active Abandoned
- 2012-07-11 EP EP12736042.8A patent/EP2732029B1/en active Active
- 2012-07-11 CA CA2841165A patent/CA2841165A1/en not_active Abandoned
-
2016
- 2016-02-23 US US15/051,541 patent/US20160281063A1/en not_active Abandoned
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