JP2014520551A - 細胞のリプログラミング方法およびゲノムの改変方法 - Google Patents

細胞のリプログラミング方法およびゲノムの改変方法 Download PDF

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JP2014520551A
JP2014520551A JP2014520269A JP2014520269A JP2014520551A JP 2014520551 A JP2014520551 A JP 2014520551A JP 2014520269 A JP2014520269 A JP 2014520269A JP 2014520269 A JP2014520269 A JP 2014520269A JP 2014520551 A JP2014520551 A JP 2014520551A
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cells
cell
nucleic acid
reprogramming
genome
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JP2014520551A5 (enExample
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トーマス バーク，
マイケル ミラー，
マイケル マクラクラン，
サラ ディカーソン，
アン ストラウス，
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セルラー ダイナミクス インターナショナル， インコーポレイテッド
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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JP2014520269A 2011-07-11 2012-07-11 細胞のリプログラミング方法およびゲノムの改変方法 Pending JP2014520551A (ja)

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US201161506314P 2011-07-11 2011-07-11
US61/506,314 2011-07-11
PCT/US2012/046194 WO2013009825A1 (en) 2011-07-11 2012-07-11 Methods for cell reprogramming and genome engineering

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JP2014520551A true JP2014520551A (ja) 2014-08-25
JP2014520551A5 JP2014520551A5 (enExample) 2015-08-13

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US (2) US20130040302A1 (enExample)
EP (1) EP2732029B1 (enExample)
JP (1) JP2014520551A (enExample)
CA (1) CA2841165A1 (enExample)
WO (1) WO2013009825A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7473209B2 (ja) 2018-01-29 2024-04-23 中国科学院動物研究所 細胞誘導の方法
US12006508B2 (en) 2012-11-01 2024-06-11 Factor Bioscience Inc. Methods and products for expressing proteins in cells

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3260140B1 (en) 2011-12-05 2021-02-03 Factor Bioscience Inc. Methods and products for transfecting cells
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
AU2014233465A1 (en) * 2013-03-15 2015-09-03 Cibus Europe B.V. Targeted gene modification using oligonucleotide-mediated gene repair
EP2792742A1 (en) 2013-04-17 2014-10-22 Universitätsklinikum Hamburg-Eppendorf (UKE) Gene-therapy vectors for treating cardiomyopathy
US10738323B2 (en) * 2013-07-12 2020-08-11 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
KR101655383B1 (ko) * 2013-07-27 2016-09-08 고려대학교 산학협력단 소분자 화합물을 포함하는 만능성 줄기세포의 염색체 안정성 유지용 조성물
KR102415811B1 (ko) 2014-01-31 2022-07-04 팩터 바이오사이언스 인크. 핵산 제조 및 송달을 위한 방법 및 산물
CN105039399A (zh) * 2014-04-23 2015-11-11 复旦大学 多能干细胞-遗传性心肌病心肌细胞及其制备方法
US10626372B1 (en) 2015-01-26 2020-04-21 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
US11241505B2 (en) 2015-02-13 2022-02-08 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
US12642852B1 (en) 2015-11-04 2026-06-02 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
CN122146606A (zh) 2015-11-04 2026-06-05 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
CA3010764A1 (en) * 2016-01-12 2017-07-20 Lonza Walkersville, Inc. Methods and vectors to produce vector free induced pluripotent stem cells
WO2017196175A1 (en) 2016-05-12 2017-11-16 Erasmus University Medical Center Rotterdam A method for culturing myogenic cells, cultures obtained therefrom, screening methods, and cell culture medium.
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
IL264439B2 (en) 2016-08-17 2024-08-01 Factor Bioscience Inc non-viral, cell-free composition comprising a synthetic messenger RNA (MRNA) encoding a gene-editing protein for use in treating cancer, and a synthetic RNA encoding a gene-editing protein for use in treatment
US20190390221A1 (en) * 2016-11-02 2019-12-26 David Kiewlich Plasmid vectors for expression of large nucleic acid transgenes
CN110891967B (zh) * 2017-04-18 2025-03-14 富士胶片细胞动力公司 抗原特异性免疫效应细胞
NL2019517B1 (en) 2017-09-08 2019-03-19 Univ Erasmus Med Ct Rotterdam New therapy for Pompe disease
CN111511902A (zh) * 2017-12-28 2020-08-07 J·大卫格莱斯顿研究所-根据J·大卫格莱斯顿遗嘱的遗嘱信托 通过crispr激活生成诱导性多能细胞
CN110016483A (zh) * 2019-04-18 2019-07-16 安徽农业大学 一种研究卵母细胞体外成熟中卵丘细胞基因功能的方法
CA3145425A1 (en) 2019-07-03 2021-01-07 Factor Bioscience Inc. Cationic lipids and uses thereof
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
FI4103214T3 (fi) 2020-02-13 2026-07-03 Tenaya Therapeutics Inc Geeniterapian vektorit sydänsairauden hoitoon
EP3922431A1 (en) 2020-06-08 2021-12-15 Erasmus University Medical Center Rotterdam Method of manufacturing microdevices for lab-on-chip applications
CN113201481B (zh) * 2021-04-19 2023-10-13 清华大学深圳国际研究生院 皮肤微球及其制备方法和应用
GB202115676D0 (en) * 2021-11-01 2021-12-15 Icahn School Med Mount Sinai Methods and compositions for bat ipsc preparation and use
WO2024091801A2 (en) * 2022-10-13 2024-05-02 President And Fellows Of Harvard College Methods and compositions for inducing cell differentiation

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009149233A1 (en) * 2008-06-04 2009-12-10 Stem Cell Products, Inc. Methods for the production of ips cells using non-viral approach
WO2009157201A1 (en) * 2008-06-26 2009-12-30 Osaka University Method and kit for preparing ips cells
WO2010048567A1 (en) * 2008-10-24 2010-04-29 Wisconsin Alumni Research Foundation Pluripotent stem cells obtained by non-viral reprogramming
WO2011056971A2 (en) * 2009-11-04 2011-05-12 Cellular Dynamics International, Inc. Episomal reprogramming with chemicals
JP2011516042A (ja) * 2008-03-17 2011-05-26 へルムホルツ・ツェントルム・ミュンヘン−ドイチェス・フォルシュングスツェントルム・ヒューア・ゲズントハイト・ウント・ウムヴェルト（ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング） 部位特異的組み換えを用いて無ベクター誘導多能性幹（ｉＰＳ）細胞を作製するベクターおよびその方法

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1088100A (en) 1913-04-08 1914-02-24 Benjamin P Saunders Apparatus for unloading freight-cars.
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0273085A1 (en) 1986-12-29 1988-07-06 IntraCel Corporation A method for internalizing nucleic acids into eukaryotic cells
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
WO1993004169A1 (en) 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
ES2148179T3 (es) 1991-09-06 2000-10-16 Yoshitomi Pharmaceutical Compuesto de 4-amino(alquil)ciclohexan-1-carboxamida y uso del mismo.
US5610042A (en) 1991-10-07 1997-03-11 Ciba-Geigy Corporation Methods for stable transformation of wheat
JP4115514B2 (ja) 1991-10-23 2008-07-09 キャンサー・リサーチ・キヤンペーン・テクノロジー・リミテツド Ｃｂ １９５４およびその類似体を細胞障害性の形態に還元するための細菌のニトロリダクターゼ
US5702932A (en) 1992-07-20 1997-12-30 University Of Florida Microinjection methods to transform arthropods with exogenous DNA
AU670316B2 (en) 1992-07-27 1996-07-11 Pioneer Hi-Bred International, Inc. An improved method of (agrobacterium)-mediated transformation of cultured soybean cells
DE4228457A1 (de) 1992-08-27 1994-04-28 Beiersdorf Ag Herstellung von heterodimerem PDGF-AB mit Hilfe eines bicistronischen Vektorsystems in Säugerzellen
GB9222888D0 (en) 1992-10-30 1992-12-16 British Tech Group Tomography
US6033907A (en) 1995-09-29 2000-03-07 Indiana University Foundation Enhanced virus-mediated DNA transfer
US5686278A (en) 1994-03-25 1997-11-11 Indiana University Foundation Methods for enhanced retrovirus-mediated gene transfer
US7083979B1 (en) 1994-03-25 2006-08-01 Indiana University Foundation Methods for enhanced retroviral-mediated gene transfer
US5656610A (en) 1994-06-21 1997-08-12 University Of Southern California Producing a protein in a mammal by injection of a DNA-sequence into the tongue
FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
US5736524A (en) 1994-11-14 1998-04-07 Merck & Co.,. Inc. Polynucleotide tuberculosis vaccine
KR100793625B1 (ko) 1995-09-29 2008-01-11 인디아나 유니버시티 리서치 앤드 테크놀로지 코퍼레이션 바이러스-매개된 ｄｎａ 전달을 향상시키는 방법에 의해생산된 생존성 세포 집단 및 이를 포함하는 세포 조성물
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
US5780448A (en) 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
US20030059913A1 (en) 1995-11-20 2003-03-27 Walter Weyler Novel Microorganisms with ability to degrade indole and novel enzymes therefrom
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5945100A (en) 1996-07-31 1999-08-31 Fbp Corporation Tumor delivery vehicles
US5981274A (en) 1996-09-18 1999-11-09 Tyrrell; D. Lorne J. Recombinant hepatitis virus vectors
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
GB0002261D0 (en) 2000-02-02 2000-03-22 Amersham Pharm Biotech Uk Ltd Fluorescent detection method & reagent
JP4458222B2 (ja) 2000-05-16 2010-04-28 独立行政法人理化学研究所 胚性幹細胞の外胚葉系細胞への新規な分化誘導法及びその用途
EP1287116A2 (en) 2000-05-17 2003-03-05 Geron Corporation Neural progenitor cell populations
JP2002184539A (ja) 2000-12-14 2002-06-28 Auto Network Gijutsu Kenkyusho:Kk コネクタ
PE20020958A1 (es) 2001-03-23 2002-11-14 Bayer Corp Inhibidores de la rho-quinasa
HN2002000067A (es) 2001-03-23 2003-10-24 Bayer Healthcare Llc Inhibidores de la rho - quinasa.
US20030211603A1 (en) 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
JP2003097552A (ja) 2001-09-21 2003-04-03 Nsk Ltd 摩擦付加装置および直動案内装置
US20030062225A1 (en) 2001-10-03 2003-04-03 Stucky Paul A. Elevator load bearing assembly having a detectable element that is indicative of local strain
EP1465900B1 (en) 2002-01-10 2008-05-14 Bayer HealthCare AG Rho-kinase inhibitors
CA2473910C (en) 2002-01-23 2011-03-15 Bayer Pharmaceuticals Corporation Pyrimidine derivatives as rho-kinase inhibitors
MXPA04007191A (es) 2002-01-23 2005-03-31 Bayer Pharmaceuticals Corp Derivados de pirimidina como inhibidores de rho-quinasa.
DE10224242A1 (de) 2002-05-29 2003-12-11 Max Delbrueck Centrum Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
US20040039796A1 (en) 2002-08-08 2004-02-26 Virtual Radio, Inc. Personalized cyber disk jockey and Internet radio advertising
JP2004295625A (ja) 2003-03-27 2004-10-21 Fujitsu Ltd エリア情報提供システム、エリア情報提供プログラム
US20050123902A1 (en) 2003-05-21 2005-06-09 President And Fellows Of Harvard College Human papillomavirus inhibitors
WO2005080554A1 (ja) 2004-02-23 2005-09-01 Kyoto University 胚性幹細胞の分化調節方法
BRPI0514641A (pt) 2004-09-08 2008-06-17 Wisconsin Alumni Res Found cultivação das células tronco embriÈnica humana
US20070116680A1 (en) 2005-11-18 2007-05-24 Rensselaer Polytechnic Institute Stem cells within gel microenvironments
WO2007113505A2 (en) 2006-03-30 2007-10-11 The University Court Of The University Of Edinburgh Culture medium containing kinase inhibitors. and uses thereof
WO2008005268A1 (en) 2006-06-30 2008-01-10 Schering Corporation Substituted piperidines that increase p53 activity and the uses thereof
CA2657227A1 (en) 2006-07-14 2008-01-17 Novartis Ag Pyrimidine derivatives as alk-5 inhibitors
SE0950586L (sv) 2007-01-17 2009-08-13 Wisconsin Alumni Res Found Förbättrad odling av stamceller
EP2126045A4 (en) 2007-01-30 2010-05-26 Univ Georgia EARLY MESODERM CELLS, STABLE POPULATION OF MESENDODERM CELLS WITH THE ABILITY TO GENERATE ENDODERM AND MESODERM CELL LINES AND MULTIPOTENTIAL MIGRATION CELLS
WO2010042490A1 (en) * 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
EP2601289B1 (en) * 2010-08-04 2017-07-12 Cellular Dynamics International, Inc. Reprogramming immortalized b cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011516042A (ja) * 2008-03-17 2011-05-26 へルムホルツ・ツェントルム・ミュンヘン−ドイチェス・フォルシュングスツェントルム・ヒューア・ゲズントハイト・ウント・ウムヴェルト（ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング） 部位特異的組み換えを用いて無ベクター誘導多能性幹（ｉＰＳ）細胞を作製するベクターおよびその方法
WO2009149233A1 (en) * 2008-06-04 2009-12-10 Stem Cell Products, Inc. Methods for the production of ips cells using non-viral approach
WO2009157201A1 (en) * 2008-06-26 2009-12-30 Osaka University Method and kit for preparing ips cells
WO2010048567A1 (en) * 2008-10-24 2010-04-29 Wisconsin Alumni Research Foundation Pluripotent stem cells obtained by non-viral reprogramming
WO2011056971A2 (en) * 2009-11-04 2011-05-12 Cellular Dynamics International, Inc. Episomal reprogramming with chemicals

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
NAT. PROTOC., vol. 5, no. 4, JPN6016018737, April 2010 (2010-04-01), pages 647 - 660, ISSN: 0003320822 *
NAT. REV. GENET., vol. 11, no. 9, JPN6016018738, September 2010 (2010-09-01), pages 636 - 646, ISSN: 0003320823 *
NATURE, vol. 458, no. 7239, JPN6016018740, 9 April 2009 (2009-04-09), pages 766 - 770, ISSN: 0003320824 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12006508B2 (en) 2012-11-01 2024-06-11 Factor Bioscience Inc. Methods and products for expressing proteins in cells
JP7473209B2 (ja) 2018-01-29 2024-04-23 中国科学院動物研究所 細胞誘導の方法

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