JP2013513360A5 - - Google Patents
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- JP2013513360A5 JP2013513360A5 JP2012542402A JP2012542402A JP2013513360A5 JP 2013513360 A5 JP2013513360 A5 JP 2013513360A5 JP 2012542402 A JP2012542402 A JP 2012542402A JP 2012542402 A JP2012542402 A JP 2012542402A JP 2013513360 A5 JP2013513360 A5 JP 2013513360A5
- Authority
- JP
- Japan
- Prior art keywords
- oligonucleotide
- amino group
- target sequence
- sequence
- nucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108091034117 Oligonucleotide Proteins 0.000 claims 36
- 125000003729 nucleotide group Chemical group 0.000 claims 25
- 239000002773 nucleotide Substances 0.000 claims 24
- 125000003277 amino group Chemical group 0.000 claims 23
- 230000000295 complement effect Effects 0.000 claims 19
- 238000000034 method Methods 0.000 claims 12
- 108020004707 nucleic acids Proteins 0.000 claims 12
- 150000007523 nucleic acids Chemical class 0.000 claims 12
- 102000039446 nucleic acids Human genes 0.000 claims 12
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims 8
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 8
- 238000003752 polymerase chain reaction Methods 0.000 claims 8
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 7
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Chemical group O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 claims 4
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims 4
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 claims 4
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Chemical group O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 claims 4
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 claims 4
- 229960005305 adenosine Drugs 0.000 claims 4
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical group O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 claims 4
- 229940029575 guanosine Drugs 0.000 claims 4
- 238000009396 hybridization Methods 0.000 claims 4
- 108700028369 Alleles Proteins 0.000 claims 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 239000002777 nucleoside Substances 0.000 claims 2
- -1 nucleoside triphosphate Chemical class 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- WWOZGLRRDHCCGH-UHFFFAOYSA-N 4-amino-4-benzyl-1,3-dihydropyrimidin-2-one Chemical compound C=1C=CC=CC=1CC1(N)NC(=O)NC=C1 WWOZGLRRDHCCGH-UHFFFAOYSA-N 0.000 claims 1
- RYDDEYJZMHLIHD-UHFFFAOYSA-N 6-[(4-tert-butylphenyl)methyl]purin-6-amine Chemical compound C(C)(C)(C)C1=CC=C(CC2(C3=NC=NC3=NC=N2)N)C=C1 RYDDEYJZMHLIHD-UHFFFAOYSA-N 0.000 claims 1
- 101100381977 Homo sapiens BRAF gene Proteins 0.000 claims 1
- 101000851181 Homo sapiens Epidermal growth factor receptor Proteins 0.000 claims 1
- 101100086477 Homo sapiens KRAS gene Proteins 0.000 claims 1
- 101000605639 Homo sapiens Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Proteins 0.000 claims 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 claims 1
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 241000204666 Thermotoga maritima Species 0.000 claims 1
- 125000003545 alkoxy group Chemical group 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 claims 1
- 102000050152 human BRAF Human genes 0.000 claims 1
- 102000057995 human PIK3CA Human genes 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 claims 1
- 125000000951 phenoxy group Chemical group [H]C1=C([H])C([H])=C(O*)C([H])=C1[H] 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 125000003107 substituted aryl group Chemical group 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28569009P | 2009-12-11 | 2009-12-11 | |
| US61/285,690 | 2009-12-11 | ||
| PCT/EP2010/007560 WO2011069677A1 (en) | 2009-12-11 | 2010-12-10 | Allele-specific amplification of nucleic acids |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015196657A Division JP2016013137A (ja) | 2009-12-11 | 2015-10-02 | 核酸の対立遺伝子特異的増幅 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013513360A JP2013513360A (ja) | 2013-04-22 |
| JP2013513360A5 true JP2013513360A5 (cg-RX-API-DMAC7.html) | 2013-07-11 |
| JP5886755B2 JP5886755B2 (ja) | 2016-03-16 |
Family
ID=43531218
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012542402A Active JP5886755B2 (ja) | 2009-12-11 | 2010-12-10 | 核酸の対立遺伝子特異的増幅 |
| JP2015196657A Pending JP2016013137A (ja) | 2009-12-11 | 2015-10-02 | 核酸の対立遺伝子特異的増幅 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015196657A Pending JP2016013137A (ja) | 2009-12-11 | 2015-10-02 | 核酸の対立遺伝子特異的増幅 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9238832B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2510117B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP5886755B2 (cg-RX-API-DMAC7.html) |
| CN (2) | CN102656278A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2781984C (cg-RX-API-DMAC7.html) |
| ES (1) | ES2530273T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2011069677A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL3246416T3 (pl) | 2011-04-15 | 2024-09-30 | The Johns Hopkins University | Bezpieczny system sekwencjonowania |
| WO2013022807A1 (en) * | 2011-08-05 | 2013-02-14 | Unitaq Bio | Methods and compositions for detection of nucleic acids using 5'-nuclease cleavage and amplification |
| KR20140064971A (ko) | 2011-09-19 | 2014-05-28 | 제넨테크, 인크. | c-met 길항제 및 B-raf 길항제를 포함하는 조합 치료 |
| US9738935B2 (en) * | 2011-11-10 | 2017-08-22 | Roche Molecular Systems, Inc. | Complex mutations in the epidermal growth factor receptor kinase domain |
| CA2889937C (en) | 2012-10-29 | 2020-12-29 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| US9382581B2 (en) | 2012-12-13 | 2016-07-05 | Roche Molecular Systems, Inc. | Primers with modified phosphate and base in allele-specific PCR |
| ES2691306T3 (es) * | 2013-03-13 | 2018-11-26 | F. Hoffmann-La Roche Ag | Procedimientos y composiciones para detectar mutaciones en el gen PI3KCA (PIK3CA) humano |
| CN103911445B (zh) * | 2014-03-20 | 2015-08-12 | 上海科亦生物科技有限公司 | 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒 |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| KR101756875B1 (ko) * | 2015-05-28 | 2017-07-11 | 에스디 바이오센서 주식회사 | 상보적 염기서열과 미스-매치된 염기서열 그리고 reporter와 quencher를 포함하는 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭 및 측정 방법 |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| US11286517B2 (en) * | 2016-02-17 | 2022-03-29 | President And Fellows Of Harvard College | Molecular programming tools |
| JP2018164421A (ja) * | 2017-03-28 | 2018-10-25 | 東洋紡株式会社 | 核酸増幅方法 |
| WO2019067092A1 (en) | 2017-08-07 | 2019-04-04 | The Johns Hopkins University | METHODS AND SUBSTANCES FOR THE EVALUATION AND TREATMENT OF CANCER |
| WO2019099420A1 (en) * | 2017-11-15 | 2019-05-23 | Yan Wang | A method for detecting multiple dna mutations and copy number variations |
| US20220073977A1 (en) | 2020-02-14 | 2022-03-10 | The Johns Hopkins University | Methods and materials for assessing nucleic acids |
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| CA2639416C (en) * | 2007-09-11 | 2019-12-31 | F. Hoffmann-La Roche Ag | Diagnostic test for susceptibility to b-raf kinase inhibitors |
| ES2527653T3 (es) | 2008-10-20 | 2015-01-28 | F. Hoffmann-La Roche Ag | Amplificación específica de alelo usando un cebador con un nucleótido modificado |
| EP2418515A1 (en) | 2010-07-21 | 2012-02-15 | Astrium GmbH | Integrity method for differential corrections |
-
2010
- 2010-12-08 US US12/962,861 patent/US9238832B2/en active Active
- 2010-12-10 ES ES10795240.0T patent/ES2530273T3/es active Active
- 2010-12-10 CN CN201080055770.6A patent/CN102656278A/zh active Pending
- 2010-12-10 CA CA2781984A patent/CA2781984C/en active Active
- 2010-12-10 CN CN201710169202.2A patent/CN106987624A/zh active Pending
- 2010-12-10 WO PCT/EP2010/007560 patent/WO2011069677A1/en not_active Ceased
- 2010-12-10 JP JP2012542402A patent/JP5886755B2/ja active Active
- 2010-12-10 EP EP10795240.0A patent/EP2510117B1/en active Active
-
2015
- 2015-10-02 JP JP2015196657A patent/JP2016013137A/ja active Pending