CN106987624A - 核酸的等位基因特异性扩增 - Google Patents

核酸的等位基因特异性扩增 Download PDF

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Publication number
CN106987624A
CN106987624A CN201710169202.2A CN201710169202A CN106987624A CN 106987624 A CN106987624 A CN 106987624A CN 201710169202 A CN201710169202 A CN 201710169202A CN 106987624 A CN106987624 A CN 106987624A
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CN
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Prior art keywords
oligonucleotides
target sequence
variant
nucleotides
nucleic acid
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CN201710169202.2A
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English (en)
Chinese (zh)
Inventor
A.赞
N.牛顿
S.G.威尔
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CN106987624A publication Critical patent/CN106987624A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201710169202.2A 2009-12-11 2010-12-10 核酸的等位基因特异性扩增 Pending CN106987624A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28569009P 2009-12-11 2009-12-11
US61/285690 2009-12-11
CN201080055770.6A CN102656278A (zh) 2009-12-11 2010-12-10 核酸的等位基因特异性扩增

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201080055770.6A Division CN102656278A (zh) 2009-12-11 2010-12-10 核酸的等位基因特异性扩增

Publications (1)

Publication Number Publication Date
CN106987624A true CN106987624A (zh) 2017-07-28

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Family Applications (2)

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CN201710169202.2A Pending CN106987624A (zh) 2009-12-11 2010-12-10 核酸的等位基因特异性扩增
CN201080055770.6A Pending CN102656278A (zh) 2009-12-11 2010-12-10 核酸的等位基因特异性扩增

Family Applications After (1)

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CN201080055770.6A Pending CN102656278A (zh) 2009-12-11 2010-12-10 核酸的等位基因特异性扩增

Country Status (7)

Country Link
US (1) US9238832B2 (cg-RX-API-DMAC7.html)
EP (1) EP2510117B1 (cg-RX-API-DMAC7.html)
JP (2) JP5886755B2 (cg-RX-API-DMAC7.html)
CN (2) CN106987624A (cg-RX-API-DMAC7.html)
CA (1) CA2781984C (cg-RX-API-DMAC7.html)
ES (1) ES2530273T3 (cg-RX-API-DMAC7.html)
WO (1) WO2011069677A1 (cg-RX-API-DMAC7.html)

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US9738935B2 (en) 2011-11-10 2017-08-22 Roche Molecular Systems, Inc. Complex mutations in the epidermal growth factor receptor kinase domain
WO2014070462A1 (en) 2012-10-29 2014-05-08 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
US9382581B2 (en) * 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
EP2971075B1 (en) * 2013-03-13 2018-08-15 Roche Diagnostics GmbH Methods and compositions for detecting mutations in the human pi3kca (pik3ca) gene
CN103911445B (zh) * 2014-03-20 2015-08-12 上海科亦生物科技有限公司 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
KR101756875B1 (ko) * 2015-05-28 2017-07-11 에스디 바이오센서 주식회사 상보적 염기서열과 미스-매치된 염기서열 그리고 reporter와 quencher를 포함하는 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭 및 측정 방법
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
US11286517B2 (en) * 2016-02-17 2022-03-29 President And Fellows Of Harvard College Molecular programming tools
JP2018164421A (ja) * 2017-03-28 2018-10-25 東洋紡株式会社 核酸増幅方法
KR20250047324A (ko) 2017-08-07 2025-04-03 더 존스 홉킨스 유니버시티 암을 평가하고 치료하기 위한 방법 및 재료
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
WO2021163546A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids

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CN101418340A (zh) * 2007-09-11 2009-04-29 霍夫曼-拉罗奇有限公司 B-raf激酶抑制剂易感性的诊断检测

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WO2003072814A2 (en) * 2002-02-26 2003-09-04 Roche Diagnostics Gmbh Improved method for allele-specific pcr
CN101418340A (zh) * 2007-09-11 2009-04-29 霍夫曼-拉罗奇有限公司 B-raf激酶抑制剂易感性的诊断检测

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JP2013513360A (ja) 2013-04-22
CA2781984C (en) 2015-02-17
EP2510117A1 (en) 2012-10-17
WO2011069677A1 (en) 2011-06-16
CA2781984A1 (en) 2011-06-16
JP2016013137A (ja) 2016-01-28
ES2530273T3 (es) 2015-02-27
CN102656278A (zh) 2012-09-05
US20110311968A1 (en) 2011-12-22
EP2510117B1 (en) 2014-12-10
US9238832B2 (en) 2016-01-19
JP5886755B2 (ja) 2016-03-16

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Application publication date: 20170728