JP2013512673A5 - - Google Patents
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- Publication number
- JP2013512673A5 JP2013512673A5 JP2012542163A JP2012542163A JP2013512673A5 JP 2013512673 A5 JP2013512673 A5 JP 2013512673A5 JP 2012542163 A JP2012542163 A JP 2012542163A JP 2012542163 A JP2012542163 A JP 2012542163A JP 2013512673 A5 JP2013512673 A5 JP 2013512673A5
- Authority
- JP
- Japan
- Prior art keywords
- hemangioblasts
- cells
- culture
- serum
- item
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 88
- 210000003566 hemangioblast Anatomy 0.000 claims description 39
- 210000004027 cell Anatomy 0.000 claims description 28
- 210000004443 dendritic cell Anatomy 0.000 claims description 28
- 239000000203 mixture Substances 0.000 claims description 25
- 102000004127 Cytokines Human genes 0.000 claims description 20
- 108090000695 Cytokines Proteins 0.000 claims description 20
- 238000012258 culturing Methods 0.000 claims description 16
- 210000000822 natural killer cell Anatomy 0.000 claims description 14
- 102000003812 Interleukin-15 Human genes 0.000 claims description 12
- 108090000172 Interleukin-15 Proteins 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 10
- 229920000609 methyl cellulose Polymers 0.000 claims description 10
- 239000001923 methylcellulose Substances 0.000 claims description 10
- 101000917858 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-A Proteins 0.000 claims description 8
- 101000917839 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-B Proteins 0.000 claims description 8
- 102100029185 Low affinity immunoglobulin gamma Fc region receptor III-B Human genes 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 8
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 claims description 4
- 102000006354 HLA-DR Antigens Human genes 0.000 claims description 4
- 108010058597 HLA-DR Antigens Proteins 0.000 claims description 4
- 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 claims description 4
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 claims description 4
- 108091036414 Polyinosinic:polycytidylic acid Proteins 0.000 claims description 4
- 210000004748 cultured cell Anatomy 0.000 claims description 4
- XEYBRNLFEZDVAW-ARSRFYASSA-N dinoprostone Chemical compound CCCCC[C@H](O)\C=C\[C@H]1[C@H](O)CC(=O)[C@@H]1C\C=C/CCCC(O)=O XEYBRNLFEZDVAW-ARSRFYASSA-N 0.000 claims description 4
- 229960002986 dinoprostone Drugs 0.000 claims description 4
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 4
- 210000003509 immature nk cell Anatomy 0.000 claims description 4
- 210000001939 mature NK cell Anatomy 0.000 claims description 4
- XEYBRNLFEZDVAW-UHFFFAOYSA-N prostaglandin E2 Natural products CCCCCC(O)C=CC1C(O)CC(=O)C1CC=CCCCC(O)=O XEYBRNLFEZDVAW-UHFFFAOYSA-N 0.000 claims description 4
- 102100035793 CD83 antigen Human genes 0.000 claims description 2
- 101000946856 Homo sapiens CD83 antigen Proteins 0.000 claims description 2
- 102100022297 Integrin alpha-X Human genes 0.000 claims description 2
- 230000035800 maturation Effects 0.000 claims description 2
- 210000002242 embryoid body Anatomy 0.000 claims 16
- 239000003102 growth factor Substances 0.000 claims 16
- 239000012679 serum free medium Substances 0.000 claims 12
- 239000002609 medium Substances 0.000 claims 7
- 238000004113 cell culture Methods 0.000 claims 5
- 102100024505 Bone morphogenetic protein 4 Human genes 0.000 claims 4
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims 4
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 4
- 101000762379 Homo sapiens Bone morphogenetic protein 4 Proteins 0.000 claims 4
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 4
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 4
- 230000004069 differentiation Effects 0.000 claims 4
- 210000000648 angioblast Anatomy 0.000 claims 3
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 claims 2
- 230000001939 inductive effect Effects 0.000 claims 1
- 230000004936 stimulating effect Effects 0.000 claims 1
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26666109P | 2009-12-04 | 2009-12-04 | |
| US61/266,661 | 2009-12-04 | ||
| PCT/US2010/058593 WO2011068896A1 (en) | 2009-12-04 | 2010-12-01 | Method of generating natural killer cells and dendritic cells from human embryonic stem cell-derived hemangioblasts |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016060101A Division JP2016119910A (ja) | 2009-12-04 | 2016-03-24 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013512673A JP2013512673A (ja) | 2013-04-18 |
| JP2013512673A5 true JP2013512673A5 (enExample) | 2014-01-16 |
| JP6013187B2 JP6013187B2 (ja) | 2016-10-25 |
Family
ID=44115272
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012542163A Active JP6013187B2 (ja) | 2009-12-04 | 2010-12-01 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
| JP2016060101A Pending JP2016119910A (ja) | 2009-12-04 | 2016-03-24 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
| JP2019022273A Active JP7343984B2 (ja) | 2009-12-04 | 2019-02-12 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
| JP2021188673A Pending JP2022027790A (ja) | 2009-12-04 | 2021-11-19 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
| JP2024154660A Pending JP2024177183A (ja) | 2009-12-04 | 2024-09-09 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016060101A Pending JP2016119910A (ja) | 2009-12-04 | 2016-03-24 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
| JP2019022273A Active JP7343984B2 (ja) | 2009-12-04 | 2019-02-12 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
| JP2021188673A Pending JP2022027790A (ja) | 2009-12-04 | 2021-11-19 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
| JP2024154660A Pending JP2024177183A (ja) | 2009-12-04 | 2024-09-09 | ヒト胚性幹細胞由来血管芽細胞からナチュラルキラー細胞および樹状細胞を生成する方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US8822218B2 (enExample) |
| EP (1) | EP2507365A4 (enExample) |
| JP (5) | JP6013187B2 (enExample) |
| KR (5) | KR101947588B1 (enExample) |
| CN (1) | CN102822332A (enExample) |
| AU (1) | AU2010326027A1 (enExample) |
| BR (1) | BR112012014104A2 (enExample) |
| CA (3) | CA3080368A1 (enExample) |
| IN (1) | IN2012DN05053A (enExample) |
| RU (1) | RU2012127811A (enExample) |
| WO (1) | WO2011068896A1 (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102651433B1 (ko) | 2006-04-14 | 2024-03-25 | 아스텔라스 인스티튜트 포 리제너러티브 메디슨 | 혈관 콜로니 형성 세포 |
| BR112012014104A2 (pt) | 2009-12-04 | 2016-07-05 | Stem Cell & Regenerative Medicine International Inc | método de geração de células destrutoras naturais e células dendríticas de hemangioblastos embrionários humanos derivados de células-tronco |
| US8961956B2 (en) | 2011-11-30 | 2015-02-24 | Ocata Therapeutics, Inc. | Mesenchymal stromal cells and uses related thereto |
| RS57886B1 (sr) | 2011-11-30 | 2019-01-31 | Astellas Inst For Regenerative Medicine | Mezenhimalne stromalne ćelije i primene vezane za njih |
| US9260696B2 (en) | 2012-04-24 | 2016-02-16 | Dan S. Kaufman | Method for developing natural killer cells from stem cells |
| CA2876499C (en) | 2012-07-12 | 2021-10-26 | Imstem Biotechnology, Inc. | Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof |
| CN114558032B (zh) | 2012-12-21 | 2025-07-29 | 安斯泰来再生医药协会 | 由多能干细胞制备血小板的方法及其组合物 |
| CN106103701B (zh) * | 2014-01-03 | 2020-04-24 | 中央研究院 | 改造的自然杀手细胞及其组成与用途 |
| CN105219710B (zh) * | 2014-06-05 | 2020-01-10 | 上海厚超生物科技有限公司 | 一种高杀伤活性的免疫细胞群的培养方法 |
| DE102014014993B4 (de) * | 2014-10-09 | 2017-02-02 | Helga Schmetzer | Verwendung von immunmodulatorisch wirksamen Kits zur immuntherapeutischen Behandlung von Patienten mit myeloischen Leukämien |
| CN104711225B (zh) * | 2015-04-09 | 2018-05-22 | 奥思达干细胞有限公司 | Nk细胞的体外制备方法 |
| SG10201913250SA (en) | 2015-08-18 | 2020-03-30 | Astellas Inst For Regenerative Medicine | Clinical formulations |
| CN105219717A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种一型极化树突状细胞及其诱导方法和应用 |
| CN105219719A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种用于激活卵巢癌特异性免疫反应的试剂盒 |
| CN105219722A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种用于激活肾癌特异性免疫反应的试剂盒 |
| CN105219723A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种用于激活胃癌特异性免疫反应的试剂盒 |
| CN105219725A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种用于激活肉瘤特异性免疫反应的试剂盒 |
| CN105219728A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种用于激活乳腺癌特异性免疫反应的试剂盒 |
| CN105219727A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种用于激活结直肠癌特异性免疫反应的试剂盒 |
| CN105219718A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种用于激活前列腺癌特异性免疫反应的试剂盒 |
| CN105219716A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种用于激活头颈部癌特异性免疫反应的试剂盒 |
| CN105219726A (zh) * | 2015-10-20 | 2016-01-06 | 上海隆耀生物科技有限公司 | 一种高效制备一型极化树突状细胞的方法及其应用 |
| KR102167548B1 (ko) * | 2017-09-21 | 2020-10-19 | 한국생명공학연구원 | 자연살해세포의 제조방법 및 그의 용도 |
| GB201810486D0 (en) | 2018-06-26 | 2018-08-08 | Imperial Innovations Ltd | Natural killer cells |
| CN113166726A (zh) | 2019-07-08 | 2021-07-23 | 南克维斯特公司 | 单核细胞源性nk细胞 |
| US11453862B2 (en) | 2019-07-08 | 2022-09-27 | Immunitybio, Inc. | Mononuclear cell derived NK cells |
| US11547726B2 (en) * | 2020-03-26 | 2023-01-10 | Honed Life Sciences, Llc | Enhancement of production of NK cells from stem cells |
| US11459372B2 (en) | 2020-11-30 | 2022-10-04 | Crispr Therapeutics Ag | Gene-edited natural killer cells |
| KR102292843B1 (ko) * | 2020-12-29 | 2021-08-25 | 주식회사 온코인사이트 | 역분화줄기세포(iPSC) 유래 자연 살해 세포 및 이의 용도 |
| US11473060B2 (en) | 2020-12-30 | 2022-10-18 | Crispr Therapeutics Ag | Compositions and methods for differentiating stem cells into NK cells |
| CN115247149B (zh) * | 2022-08-22 | 2023-06-16 | 华域生物科技(天津)有限公司 | 适用于nk细胞的培养基组合物及培养方法 |
| CN120659871A (zh) * | 2022-09-02 | 2025-09-16 | 帝国理工学院创新有限公司 | 自然杀伤细胞 |
| KR20250127134A (ko) | 2022-12-29 | 2025-08-26 | 아스텔라스세이야쿠 가부시키가이샤 | 조작된 자연 살상 세포 및 관련 방법 |
| WO2025212519A1 (en) | 2024-04-01 | 2025-10-09 | Moonlight Bio, Inc. | Dll3 binding proteins and uses thereof |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0765398B1 (en) * | 1994-06-15 | 2003-06-04 | Systemix, Inc. | Population of cells enriched for myeloid and/or lymphoid progenitors and methods of making and using |
| JP2002515756A (ja) * | 1997-01-31 | 2002-05-28 | ヘモソル インク． | 選択されたリンパ球の生産方法 |
| WO1998053048A1 (en) * | 1997-05-21 | 1998-11-26 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Methods and compositions for making dendritic cells from expanded populations of monocytes and for activating t cells |
| JP2001525176A (ja) * | 1997-12-04 | 2001-12-11 | デューク・ユニバーシティー | Ｃｄ７＋ｃｄ３４−ｌｉｎ−造血細胞を単離し、使用する方法 |
| FR2801056B1 (fr) * | 1999-11-12 | 2003-03-28 | Commissariat Energie Atomique | Proteine presente a la surface des cellules souches hematopoietiques de la lignee lymphoide et des cellules nk, et ses applications |
| KR20020083634A (ko) * | 2001-04-27 | 2002-11-04 | 크레아젠 주식회사 | 수지상 세포를 포함하는 자가면역 질환의 치료용 약제학적조성물 및 이를 이용한 치료방법 |
| US20050042751A1 (en) * | 2001-10-31 | 2005-02-24 | Michel Goldman | Generation and use of new types of dendritic cells |
| WO2004050855A2 (en) * | 2002-12-04 | 2004-06-17 | Baylor Research Institute | Rapid one-step method for generation of antigen loaded dendritic cell vaccine from precursors |
| US20050032210A1 (en) * | 2003-03-18 | 2005-02-10 | Kirin Beer Kabushiki Kaisha | Method of preparing immuno-regulatory dendritic cells and the use thereof |
| EP2263698A1 (en) * | 2003-04-01 | 2010-12-22 | United States of America Dempartment of Veteran's Affairs | Stem-cell, precursor cell, or target cell-based treatment of multi-organ failure and renal dysfunction |
| US20070141703A1 (en) * | 2003-11-19 | 2007-06-21 | Stanley Edouard G | Methods for producing blood products from pluripotent cells in cell culture |
| WO2005085426A1 (ja) * | 2004-03-04 | 2005-09-15 | Tanabe Seiyaku Co., Ltd. | 無フィ−ダ−分化用培地及び霊長類動物胚性幹細胞からの無フィ−ダ−分化方法 |
| CA2571844A1 (en) * | 2004-06-24 | 2006-01-05 | Dnavec Research Inc. | Anticancer agent containing minus-strand rna virus |
| US20080145931A1 (en) * | 2004-10-07 | 2008-06-19 | Argos Therapeutics, Inc. | Mature Dendritic Cell Compositions and Methods of Culturing Same |
| US20080166751A1 (en) * | 2005-02-23 | 2008-07-10 | Takayuki Asahara | Method of Analyzing Dynamics in the Differentiation of Vascular Endothelial Progenitor Cells |
| KR20150032915A (ko) * | 2005-04-08 | 2015-03-30 | 아르고스 쎄라퓨틱스 인코포레이티드 | 수지상 세포 조성물 및 방법 |
| GB2440494B (en) * | 2005-06-01 | 2010-07-28 | Wisconsin Alumni Res Found | Method of forming dendritic from embryonic stem cells |
| DK1941027T3 (da) * | 2005-09-28 | 2014-10-13 | Ipd Therapeutics B V | Fremgangsmåder og midler til stamcelleforøgelse og efterfølgende generering og ekspansion af progenitorceller samt produktion af effektorceller som kliniske terapeutika |
| KR102651433B1 (ko) * | 2006-04-14 | 2024-03-25 | 아스텔라스 인스티튜트 포 리제너러티브 메디슨 | 혈관 콜로니 형성 세포 |
| WO2008038279A2 (en) * | 2006-09-25 | 2008-04-03 | Kfir Nissan | Force transducer and method |
| EP2088190A4 (en) | 2006-11-09 | 2011-01-05 | Japan Government | METHOD FOR THE CULTURE AND PASSING OF AN EMBRYONAL PRIMATIVE STEM CELL AND METHOD FOR INDUCING THE DIFFERENTIATION OF THE EMBRYONAL STEM CELL |
| DE102006054041B3 (de) * | 2006-11-16 | 2008-05-08 | Pierburg Gmbh | Regelvorrichtung für eine Verbrennungskraftmaschine |
| CA2700617C (en) * | 2007-09-28 | 2018-11-06 | Celgene Cellular Therapeutics | Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells |
| WO2009120891A2 (en) * | 2008-03-27 | 2009-10-01 | Geron Corporation | Differentiation of primate pluripotent stem cells to hematopoietic lineage cells |
| KR20210003301A (ko) * | 2008-05-06 | 2021-01-11 | 아스텔라스 인스티튜트 포 리제너러티브 메디슨 | 다능성 줄기세포로부터 유도된 탈핵 적혈구계 세포를 생산하는 방법 |
| EP2712921B1 (en) | 2008-05-06 | 2018-07-11 | Astellas Institute for Regenerative Medicine | Hemangio colony forming cells and non-engrafting hemangio cells |
| CN102388130B (zh) * | 2009-02-27 | 2014-08-27 | 细胞动力国际有限公司 | 多能细胞的分化 |
| BR112012014104A2 (pt) | 2009-12-04 | 2016-07-05 | Stem Cell & Regenerative Medicine International Inc | método de geração de células destrutoras naturais e células dendríticas de hemangioblastos embrionários humanos derivados de células-tronco |
-
2010
- 2010-12-01 BR BR112012014104A patent/BR112012014104A2/pt not_active IP Right Cessation
- 2010-12-01 RU RU2012127811/10A patent/RU2012127811A/ru not_active Application Discontinuation
- 2010-12-01 CA CA3080368A patent/CA3080368A1/en not_active Abandoned
- 2010-12-01 US US13/512,795 patent/US8822218B2/en active Active
- 2010-12-01 EP EP10835074.5A patent/EP2507365A4/en not_active Withdrawn
- 2010-12-01 AU AU2010326027A patent/AU2010326027A1/en not_active Abandoned
- 2010-12-01 KR KR1020127017370A patent/KR101947588B1/ko active Active
- 2010-12-01 JP JP2012542163A patent/JP6013187B2/ja active Active
- 2010-12-01 KR KR1020207018660A patent/KR20200083645A/ko not_active Ceased
- 2010-12-01 WO PCT/US2010/058593 patent/WO2011068896A1/en not_active Ceased
- 2010-12-01 KR KR1020197003655A patent/KR20190016602A/ko not_active Ceased
- 2010-12-01 KR KR1020257009314A patent/KR20250048382A/ko active Pending
- 2010-12-01 CA CA3095576A patent/CA3095576A1/en active Pending
- 2010-12-01 KR KR1020217030824A patent/KR20210120136A/ko not_active Ceased
- 2010-12-01 IN IN5053DEN2012 patent/IN2012DN05053A/en unknown
- 2010-12-01 CA CA2781969A patent/CA2781969A1/en not_active Abandoned
- 2010-12-01 CN CN2010800629687A patent/CN102822332A/zh active Pending
-
2014
- 2014-08-21 US US14/465,533 patent/US20150140657A1/en not_active Abandoned
-
2016
- 2016-03-24 JP JP2016060101A patent/JP2016119910A/ja active Pending
-
2019
- 2019-02-12 JP JP2019022273A patent/JP7343984B2/ja active Active
- 2019-10-31 US US16/669,931 patent/US20200131475A1/en active Pending
-
2021
- 2021-11-19 JP JP2021188673A patent/JP2022027790A/ja active Pending
-
2024
- 2024-09-09 JP JP2024154660A patent/JP2024177183A/ja active Pending
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