JP2013094161A - 腱細胞シート及びその製造方法 - Google Patents
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Abstract
【解決手段】伸縮可能な細胞培養用基材表面上で培養し、培養期間中、当該基材を延伸、収縮、或いはそれらを繰り返すこと。
【選択図】なし
Description
Claims (10)
- 細胞培養用基材を延伸、収縮、或いはそれらを繰り返すことでその基材表面上で培養された、腱細胞シート。
- 腱細胞が、成熟腱細胞、腱前駆細胞、腱組織細胞のいずれか、若しくは2種以上の混合物である、請求項1記載の腱細胞シート。
- 基材が伸縮性を有する材質である、請求項1、2のいずれか1項記載の腱細胞シート。
- 基材の材質がポリジメチルシロキサンである、請求項3記載の腱細胞シート。
- 細胞培養基材表面に0〜80℃の温度範囲内で水和力が変化する温度応答性ポリマーが被覆されたものである、請求項1〜4のいずれか1項記載の腱細胞シート。
- 基材表面に被覆された温度応答性ポリマーが、ポリ−N−置換アクリルアミド誘導体、ポリ−N−置換メタアクリルアミド誘導体、ポリアクリレート誘導体、ポリメタクリレート誘導体の単独、もしくはこれらの2つ以上の共重合体からなる、請求項1〜5のいずれか1項記載の腱細胞シート。
- 温度応答性ポリマーが、ポリ−N−イソプロピルアクリルアミドである、請求項6記載の腱細胞シート。
- 伸縮可能な細胞培養用基材表面上で培養し、培養期間中、当該基材を延伸、収縮、或いはそれらを繰り返すことを特徴とする腱細胞シートの製造方法。
- 基材の延伸、収縮、或いはそれらを繰り返すことが、細胞が基材表面に付着した直後から行われる、請求項8記載の腱細胞シートの製造方法。
- 請求項5〜7のいずれかの細胞培養用基材表面に被覆された温度応答性ポリマー層の性質の変化を利用することで剥離させる、請求項8、9のいずれか1項記載の腱細胞シートの製造方法。
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2015109812A (ja) * | 2013-12-06 | 2015-06-18 | 大日本印刷株式会社 | 温度応答性を有する細胞培養基材およびその製造方法 |
JP2018143761A (ja) * | 2017-03-01 | 2018-09-20 | 多木化学株式会社 | 伸縮性コラーゲンシート |
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