JP2012531913A5 - - Google Patents

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JP2012531913A5
JP2012531913A5 JP2012518572A JP2012518572A JP2012531913A5 JP 2012531913 A5 JP2012531913 A5 JP 2012531913A5 JP 2012518572 A JP2012518572 A JP 2012518572A JP 2012518572 A JP2012518572 A JP 2012518572A JP 2012531913 A5 JP2012531913 A5 JP 2012531913A5
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tag sequence
nucleic acid
sequence
universal
target
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JP2012531913A (ja
JP5785942B2 (ja
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JP2012518572A 2009-06-29 2010-06-29 ヘアピンコンフォメーションを有するキメラプライマーおよびその使用法 Active JP5785942B2 (ja)

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US22127109P 2009-06-29 2009-06-29
US61/221,271 2009-06-29
PCT/US2010/040354 WO2011008530A2 (en) 2009-06-29 2010-06-29 Chimeric primers with hairpin conformations and methods of using same

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JP2012531913A JP2012531913A (ja) 2012-12-13
JP2012531913A5 true JP2012531913A5 (enExample) 2013-06-27
JP5785942B2 JP5785942B2 (ja) 2015-09-30

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JP2012518572A Active JP5785942B2 (ja) 2009-06-29 2010-06-29 ヘアピンコンフォメーションを有するキメラプライマーおよびその使用法
JP2015147338A Active JP6270786B2 (ja) 2009-06-29 2015-07-27 ヘアピンコンフォメーションを有するキメラプライマーおよびその使用法
JP2017248991A Active JP6591521B2 (ja) 2009-06-29 2017-12-26 ヘアピンコンフォメーションを有するキメラプライマーおよびその使用法

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JP2017248991A Active JP6591521B2 (ja) 2009-06-29 2017-12-26 ヘアピンコンフォメーションを有するキメラプライマーおよびその使用法

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US (5) US9587270B2 (enExample)
EP (2) EP2789689B1 (enExample)
JP (3) JP5785942B2 (enExample)
CN (1) CN102686728B (enExample)
CA (1) CA2766351C (enExample)
WO (1) WO2011008530A2 (enExample)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007029772B4 (de) * 2007-06-22 2011-12-08 Aj Innuscreen Gmbh Verfahren und Schnelltest zum Nachweis spezifischer Nukleinsäuresequenzen
CN102639714A (zh) * 2009-10-29 2012-08-15 日本碍子株式会社 靶核酸的检测方法
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
JP5851496B2 (ja) * 2010-06-14 2016-02-03 ナショナル ユニヴァーシティー オブ シンガポール 修飾ステムループオリゴヌクレオチドが仲介する逆転写および塩基間隔が制限された定量的pcr
CN103228785B (zh) * 2010-11-24 2016-09-07 株式会社钟化 扩增核酸的检测方法和检测装置
EP2762562B1 (en) * 2011-09-14 2019-05-22 NGK Insulators, Ltd. Method for detecting target nucleic acid
CN108192952A (zh) 2011-09-29 2018-06-22 露美内克丝公司 水解探针
US10385392B2 (en) * 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
CA2865575C (en) * 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
CN104254620B (zh) * 2012-04-27 2022-11-08 株式会社钟化 核酸的扩增方法及扩增核酸的检测方法
WO2014062717A1 (en) * 2012-10-15 2014-04-24 Life Technologies Corporation Compositions, methods, systems and kits for target nucleic acid enrichment
EP3988671A1 (en) * 2013-02-20 2022-04-27 Emory University Compositions for sequencing nucleic acids in mixtures
EP4697022A2 (en) * 2013-08-09 2026-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
CN105745528A (zh) 2013-10-07 2016-07-06 赛卢拉研究公司 用于以数字方式对阵列上的特征进行计数的方法和系统
JP6691380B2 (ja) 2013-11-22 2020-04-28 株式会社カネカ 短鎖rnaの検出方法
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US10947588B2 (en) * 2014-06-19 2021-03-16 Multiplicom N.V. Restriction mediated quantitative polymerase chain reactions
WO2016025815A1 (en) 2014-08-14 2016-02-18 Luminex Corporation Cleavable hairpin primers
WO2016134078A1 (en) 2015-02-19 2016-08-25 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
US10557134B2 (en) * 2015-02-24 2020-02-11 Trustees Of Boston University Protection of barcodes during DNA amplification using molecular hairpins
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
CA3019589A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
CN109074430B (zh) 2016-05-26 2022-03-29 贝克顿迪金森公司 分子标记计数调整方法
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11608497B2 (en) 2016-11-08 2023-03-21 Becton, Dickinson And Company Methods for cell label classification
JP7232180B2 (ja) 2016-11-08 2023-03-02 ベクトン・ディキンソン・アンド・カンパニー 発現プロファイル分類の方法
JP7104048B2 (ja) 2017-01-13 2022-07-20 セルラー リサーチ, インコーポレイテッド 流体チャネルの親水性コーティング
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US10941445B2 (en) 2017-03-24 2021-03-09 Bio-Rad Laboratories, Inc. Universal hairpin primers
CN108546748A (zh) * 2017-04-05 2018-09-18 杭州丹威生物科技有限公司 一种检测核酸的方法和试剂盒
CN108690875A (zh) * 2017-04-05 2018-10-23 杭州丹威生物科技有限公司 用来筛查传染病基因和遗传改变的带有条码的微阵列芯片及使用方法
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
KR101899371B1 (ko) * 2017-07-25 2018-10-29 (주)엔바이오텍 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
JP2021510085A (ja) * 2018-01-05 2021-04-15 クオシェント スイッス エスエー 自己組織型診断アレイプラットフォーム
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
CA3097938A1 (en) * 2018-05-04 2019-11-07 The Regents Of The University Of California Spiked primers for enrichment of pathogen nucleic acids among background of nucleic acids
CN118853827A (zh) 2018-10-01 2024-10-29 贝克顿迪金森公司 确定5’转录物序列
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
EP3894552B1 (en) 2018-12-13 2026-01-28 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
CN109852612A (zh) * 2019-02-26 2019-06-07 上海翼和应用生物技术有限公司 一种结合通用荧光引物的发夹修饰引物
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
CN114651070A (zh) * 2019-09-16 2022-06-21 精密科学公司 结构和温度依赖性瓣状核酸内切酶底物
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
EP4090763B1 (en) 2020-01-13 2024-12-04 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US20230227896A1 (en) * 2020-01-16 2023-07-20 Dnae Diagnostics Limited Compositions, Kits and Methods for Isolating Target Polynucleotides
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021188186A2 (en) * 2020-03-18 2021-09-23 The Board Of Trustees Of The Leland Stanford Junior University Detection of gene polymorphisms
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
WO2021247593A1 (en) 2020-06-02 2021-12-09 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
KR20230098259A (ko) * 2020-10-29 2023-07-03 비오까르띠 엔브이 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
JP6972291B1 (ja) * 2020-12-24 2021-11-24 栄研化学株式会社 核酸増幅方法、核酸増幅方法用プライマーセット、プローブ、及びキット
CN117683855B (zh) * 2024-01-29 2024-05-17 杭州布平医学检验实验室有限公司 基因突变参考品的制备方法及其应用

Family Cites Families (139)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4498766A (en) 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4767206A (en) * 1984-12-24 1988-08-30 Flow Cytometry Standards Corporation Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof
US4857451A (en) 1984-12-24 1989-08-15 Flow Cytometry Standards Corporation Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor
US4774189A (en) 1984-12-24 1988-09-27 Flow Cytometry Standards Corp. Fluorescent calibration microbeads simulating stained cells
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4989977A (en) * 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
US4682195A (en) 1985-09-30 1987-07-21 General Electric Company Insulated gate device with configured emitter contact pad
US4959463A (en) 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
US4659774A (en) 1985-11-01 1987-04-21 American Hoechst Corporation Support for solid-phase oligonucleotide synthesis
US4714682A (en) 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells
EP0232967B1 (en) 1986-01-10 1993-04-28 Amoco Corporation Competitive homogeneous assay
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
EP0266032A1 (en) 1986-08-29 1988-05-04 Beecham Group Plc Modified fibrinolytic enzyme
AU622104B2 (en) 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
US4816571A (en) 1987-06-04 1989-03-28 Applied Biosystems, Inc. Chemical capping by phosphitylation during oligonucleotide synthesis
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
US4942124A (en) 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
EP0359789B1 (en) 1988-01-21 1993-08-04 Genentech, Inc. Amplification and detection of nucleic acid sequences
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5932413A (en) 1988-04-01 1999-08-03 Celebuski; Joseph Eugene DNA probe assay using neutrally charged probe strands
US5602244A (en) 1988-05-26 1997-02-11 Competitive Technologies, Inc. Polynucleotide phosphorodithioate compounds
US5858652A (en) 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US4932207A (en) 1988-12-28 1990-06-12 Sundstrand Corporation Segmented seal plate for a turbine engine
US5856092A (en) 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
US5141813A (en) 1989-08-28 1992-08-25 Clontech Laboratories, Inc. Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes
US5137806A (en) 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
US5160974A (en) * 1990-06-25 1992-11-03 Flow Science, Inc. Closed sample cell for use in flow cytometry
US5645987A (en) 1990-09-21 1997-07-08 Amgen Inc. Enzymatic synthesis of oligonucleotides
US5478722A (en) * 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5719262A (en) 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5714331A (en) 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5766855A (en) 1991-05-24 1998-06-16 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity and sequence specificity
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
DE4204650C1 (enExample) 1992-02-15 1993-07-08 Hoffmeister, Helmut, Dr., 4400 Muenster, De
EP0675966B1 (en) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
EP0566751B1 (en) * 1992-03-23 1996-01-10 F. Hoffmann-La Roche Ag DNA detection method
EP0672181B1 (en) 1992-04-01 2003-11-05 The Johns Hopkins University School Of Medicine Methods of detecting mammalian nucleic acids isolated from stool specimen and reagents therefor
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
GB9211979D0 (en) 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5843640A (en) 1992-06-19 1998-12-01 Northwestern University Method of simultaneously detecting amplified nucleic acid sequences and cellular antigens in cells
US5861244A (en) 1992-10-29 1999-01-19 Profile Diagnostic Sciences, Inc. Genetic sequence assay using DNA triple strand formation
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
GB9311386D0 (en) 1993-06-02 1993-07-21 Pna Diagnostics As Nucleic acid analogue assay procedures
US5846709A (en) 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
EP0663447B1 (en) 1993-12-28 2003-07-09 Eiken Chemical Co., Ltd. Method of detecting a specific polynucleotide
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
EP0763135B1 (en) 1994-05-28 2002-07-10 Tepnel Medical Limited Producing copies of nucleic acids
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
WO1996006190A2 (en) 1994-08-19 1996-02-29 Perkin-Elmer Corporation Coupled amplification and ligation method
US5574146A (en) 1994-08-30 1996-11-12 Beckman Instruments, Inc. Oligonucleotide synthesis with substituted aryl carboxylic acids as activators
US5599668A (en) 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
US5554744A (en) 1994-09-23 1996-09-10 Hybridon, Inc. Method for loading solid supports for nucleic acid synthesis
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
ATE340866T1 (de) 1994-10-28 2006-10-15 Gen Probe Inc Zusammensetzungen und verfahren für die gleichzeitige detektion und quantifizierung von einer mehrheit spezifischer nuklein säure sequenzen
US5935825A (en) 1994-11-18 1999-08-10 Shimadzu Corporation Process and reagent for amplifying nucleic acid sequences
US5843650A (en) 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
US5929227A (en) 1995-07-12 1999-07-27 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5866331A (en) 1995-10-20 1999-02-02 University Of Massachusetts Single molecule detection by in situ hybridization
US5705629A (en) 1995-10-20 1998-01-06 Hybridon, Inc. Methods for H-phosphonate synthesis of mono- and oligonucleotides
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
CA2244891C (en) 1996-02-09 2008-12-30 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
US5912124A (en) 1996-06-14 1999-06-15 Sarnoff Corporation Padlock probe detection
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
CA2744096C (en) 1996-07-31 2013-07-30 Laboratory Corporation Of America Holdings Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US5853992A (en) 1996-10-04 1998-12-29 The Regents Of The University Of California Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels
US5853993A (en) 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US5908845A (en) 1996-10-30 1999-06-01 Segev; David Polyether nucleic acids
US5905024A (en) 1996-12-17 1999-05-18 University Of Chicago Method for performing site-specific affinity fractionation for use in DNA sequencing
US5846729A (en) 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5928869A (en) 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5866366A (en) 1997-07-01 1999-02-02 Smithkline Beecham Corporation gidB
US5916776A (en) 1997-08-27 1999-06-29 Sarnoff Corporation Amplification method for a polynucleotide
US5935791A (en) 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
GB9902971D0 (en) 1999-02-11 1999-03-31 Zeneca Ltd Assay
US6964847B1 (en) * 1999-07-14 2005-11-15 Packard Biosciences Company Derivative nucleic acids and uses thereof
US6287778B1 (en) 1999-10-19 2001-09-11 Affymetrix, Inc. Allele detection using primer extension with sequence-coded identity tags
US7838225B2 (en) * 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
ATE411397T1 (de) * 2000-02-07 2008-10-15 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
US20050106564A1 (en) * 2000-06-02 2005-05-19 Hechinger Mark K. One-step, no-wash multiplex bead-based flow cytometric assay
WO2001094625A2 (en) 2000-06-06 2001-12-13 Tm Bioscience Corporation Capture moieties for nucleic acids and uses thereof
JP4588976B2 (ja) 2001-01-25 2010-12-01 ルミネックス モレキュラー ダイアグノスティックス, インク. タグおよびタグ相補体としてのポリヌクレオチド、製品、ならびにその使用法
US7226737B2 (en) 2001-01-25 2007-06-05 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
EP1439222A1 (en) 2001-10-26 2004-07-21 Matsushita Electric Industrial Co., Ltd. Method of detecting target nucleic acid and nucleic acid probe
US6803201B2 (en) * 2002-01-24 2004-10-12 Stratagene Compositions and methods for polynucleotide sequence determination
JP2004205414A (ja) 2002-12-26 2004-07-22 Institute Of Physical & Chemical Research 核酸マイクロアレイ、その作製方法及びその使用方法
US7700750B2 (en) * 2003-01-09 2010-04-20 Third Wave Technologies, Inc. Connexin allele detection assays
JP2004283024A (ja) 2003-03-19 2004-10-14 Junsei Chemical Co Ltd 新規アンチセンスオリゴヌクレオチド及び抗hiv剤
CN1836050A (zh) * 2003-07-07 2006-09-20 单细胞系统公司 发夹标记的探针及其使用方法
WO2005010199A2 (en) 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
WO2005047533A1 (en) 2003-11-17 2005-05-26 Tm Bioscience Corporation Method of detecting mutations associated with thrombosis
JP2005253427A (ja) * 2004-03-15 2005-09-22 Aisin Seiki Co Ltd 核酸検出方法及び核酸単離方法
CA2479968A1 (en) 2004-09-01 2006-03-01 Keith R. Walley Protein c and endothelial protein c receptor polymorphisms as indicators of patient outcome
ES2292270B1 (es) * 2004-04-14 2009-02-16 Oryzon Genomics, S.A. Procedimiento para detectar selectivamente acidos nucleicos con estructuras atipicas convertibles en muescas.
AU2005249154B2 (en) * 2004-06-01 2011-02-10 Luminex Molecular Diagnostics, Inc. Method of detecting cystic fibrosis associated mutations
ATE471991T1 (de) 2004-06-30 2010-07-15 Luminex Molecular Diagnostics Verfahren zum nachweis von mutationen im für cytochrom p450-2d6 codierenden gen
CA2571817A1 (en) 2004-06-30 2006-01-12 Tm Bioscience Pgx, Inc. Method of detecting mutations in the gene encoding cytochrome p450-2c9
US20070259356A1 (en) * 2004-11-04 2007-11-08 Applera Corporation Concatameric Ligation Products: Compositions, Methods and Kits for Same
US8883487B2 (en) * 2004-12-23 2014-11-11 Abbott Point Of Care Inc. Molecular diagnostics system and methods
WO2007011946A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Detection of nucleic acid amplification
EP1984546A4 (en) 2006-01-24 2009-09-23 Univ British Columbia POLYMORPHISMS OF THE VASOPRESSIN SIGNALING PATH AS INDICATORS OF THE EVOLUTION OF STATUS OF SUBJECTS IN SUBJECTS SERIOUSLY REACHED
US20070172841A1 (en) 2006-01-25 2007-07-26 Hui Wang Probe/target stabilization with add-in oligo
JP2008048648A (ja) 2006-08-23 2008-03-06 Institute Of Physical & Chemical Research 核酸増幅用プライマーセット及び核酸の増幅方法
WO2008045251A2 (en) * 2006-10-04 2008-04-17 Third Wave Technologies, Inc. Snap-back primers and detectable hairpin structures
KR101433208B1 (ko) * 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 pcr의 다중 분석을 위한 시스템과 방법
US7862999B2 (en) * 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
WO2008152145A1 (de) 2007-06-13 2008-12-18 Attomol Gmbh Molekulare Diagnostika Verfahren zur durchführung und auswertung von mix & measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat
EP2215245A2 (en) * 2007-11-02 2010-08-11 Luminex Molecular Diagnostics, Inc. One-step target detection assay
US8008019B2 (en) * 2007-11-28 2011-08-30 Luminex Molecular Diagnostics Use of dual-tags for the evaluation of genomic variable repeat regions
JP4744503B2 (ja) * 2007-11-30 2011-08-10 シャープ株式会社 操作処理装置
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
FR2957821B1 (fr) 2010-03-24 2014-08-29 Inst Francais Du Petrole Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives

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