JP2014506485A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2014506485A5 JP2014506485A5 JP2013555562A JP2013555562A JP2014506485A5 JP 2014506485 A5 JP2014506485 A5 JP 2014506485A5 JP 2013555562 A JP2013555562 A JP 2013555562A JP 2013555562 A JP2013555562 A JP 2013555562A JP 2014506485 A5 JP2014506485 A5 JP 2014506485A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- acid molecule
- rna polymerase
- target nucleic
- tag
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 71
- 108020004707 nucleic acids Proteins 0.000 claims description 58
- 150000007523 nucleic acids Chemical class 0.000 claims description 58
- 102000039446 nucleic acids Human genes 0.000 claims description 58
- 238000012163 sequencing technique Methods 0.000 claims description 49
- 239000002777 nucleoside Substances 0.000 claims description 47
- -1 nucleoside triphosphates Chemical class 0.000 claims description 47
- 235000011178 triphosphate Nutrition 0.000 claims description 47
- 239000001226 triphosphate Substances 0.000 claims description 47
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 43
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 39
- 239000007790 solid phase Substances 0.000 claims description 21
- 239000000758 substrate Substances 0.000 claims description 20
- 239000002253 acid Substances 0.000 claims description 7
- 241001515965 unidentified phage Species 0.000 claims description 5
- 108020004513 Bacterial RNA Proteins 0.000 claims description 4
- 101710137500 T7 RNA polymerase Proteins 0.000 claims description 3
- 239000012472 biological sample Substances 0.000 claims description 3
- 241000588724 Escherichia coli Species 0.000 claims description 2
- 241000206602 Eukaryota Species 0.000 claims description 2
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 claims description 2
- 230000005684 electric field Effects 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 description 12
- 239000000872 buffer Substances 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 239000012148 binding buffer Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161463850P | 2011-02-23 | 2011-02-23 | |
| US61/463,850 | 2011-02-23 | ||
| US201161574270P | 2011-07-30 | 2011-07-30 | |
| US61/574,270 | 2011-07-30 | ||
| PCT/US2012/026339 WO2012116191A1 (en) | 2011-02-23 | 2012-02-23 | Rotation-dependent transcriptional sequencing systems and methods of using |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014506485A JP2014506485A (ja) | 2014-03-17 |
| JP2014506485A5 true JP2014506485A5 (enExample) | 2015-04-09 |
| JP6018092B2 JP6018092B2 (ja) | 2016-11-02 |
Family
ID=45852700
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013555562A Expired - Fee Related JP6018092B2 (ja) | 2011-02-23 | 2012-02-23 | 回転依存転写配列決定システムおよび使用方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US8574840B2 (enExample) |
| EP (1) | EP2678444B1 (enExample) |
| JP (1) | JP6018092B2 (enExample) |
| KR (1) | KR101924665B1 (enExample) |
| CN (1) | CN103534357B (enExample) |
| AU (1) | AU2012220546B2 (enExample) |
| CA (1) | CA2827880A1 (enExample) |
| RU (1) | RU2636460C2 (enExample) |
| WO (1) | WO2012116191A1 (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9128981B1 (en) | 2008-07-29 | 2015-09-08 | James L. Geer | Phone assisted ‘photographic memory’ |
| US8775454B2 (en) | 2008-07-29 | 2014-07-08 | James L. Geer | Phone assisted ‘photographic memory’ |
| KR101924665B1 (ko) | 2011-02-23 | 2018-12-03 | 이브 바이오메디컬, 인크. | 회전-의존성 전사 서열분석 시스템 및 사용 방법 |
| JP2016507249A (ja) * | 2013-02-20 | 2016-03-10 | イブ バイオメディカル インコーポレイテッド | ナノ構造ベースの核酸配列決定のための方法および組成物 |
| WO2015103566A2 (en) * | 2014-01-06 | 2015-07-09 | The Regents Of The University Of California | Spatial frequency domain imaging using custom patterns |
| ES2697428T3 (es) | 2014-07-15 | 2019-01-23 | Illumina Inc | Dispositivo electrónico activado bioquímicamente |
| WO2016174271A1 (en) * | 2015-04-30 | 2016-11-03 | Curevac Ag | Immobilized poly(n)polymerase |
| CN106991224A (zh) * | 2017-03-27 | 2017-07-28 | 蔡彤� | 一种芯片引线键合定位的建模方法及系统 |
| US10273528B1 (en) | 2017-11-17 | 2019-04-30 | Ultima Genomics, Inc. | Methods and systems for analyte detection and analysis |
| US11499962B2 (en) | 2017-11-17 | 2022-11-15 | Ultima Genomics, Inc. | Methods and systems for analyte detection and analysis |
| US10885772B2 (en) * | 2017-12-21 | 2021-01-05 | Lumileds Llc | Road lighting |
| US11798406B2 (en) * | 2018-03-21 | 2023-10-24 | Lumileds Llc | Road lighting |
| US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| US10512911B1 (en) | 2018-12-07 | 2019-12-24 | Ultima Genomics, Inc. | Implementing barriers for controlled environments during sample processing and detection |
| US12239980B2 (en) | 2018-12-07 | 2025-03-04 | Ultima Genomics, Inc. | Implementing barriers for controlled environments during sample processing and detection |
| US10852518B1 (en) | 2019-03-14 | 2020-12-01 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US10900078B2 (en) | 2019-03-14 | 2021-01-26 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US11118223B2 (en) | 2019-03-14 | 2021-09-14 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US10830703B1 (en) | 2019-03-14 | 2020-11-10 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| CN113905822B (zh) * | 2019-04-12 | 2023-09-22 | 西部数据技术公司 | 使用磁传感器阵列边合成边核酸测序 |
| US11112468B2 (en) | 2019-04-12 | 2021-09-07 | Western Digital Technologies, Inc. | Magnetoresistive sensor array for molecule detection and related detection schemes |
| US11609208B2 (en) | 2019-04-12 | 2023-03-21 | Western Digital Technologies, Inc. | Devices and methods for molecule detection based on thermal stabilities of magnetic nanoparticles |
| US11327073B2 (en) | 2019-04-12 | 2022-05-10 | Western Digital Technologies, Inc. | Thermal sensor array for molecule detection and related detection schemes |
| CN110376171B (zh) * | 2019-07-15 | 2021-11-19 | 上海理工大学 | 应用于dPCR检测仪的透射式荧光检测成像系统 |
| US11208682B2 (en) | 2019-09-13 | 2021-12-28 | Western Digital Technologies, Inc. | Enhanced optical detection for nucleic acid sequencing using thermally-dependent fluorophore tags |
| US11060138B1 (en) | 2020-01-17 | 2021-07-13 | Element Biosciences, Inc. | Nucleic acid sequencing systems |
| CA3195982A1 (en) * | 2020-10-23 | 2022-04-28 | Spindle Biotech Inc. | Compositions and methods for rna synthesis |
| GB2629259B (en) | 2021-07-21 | 2025-05-28 | Element Biosciences Inc | Optical systems for nucleic acid sequencing and methods thereof |
| KR20250130601A (ko) | 2022-11-29 | 2025-09-02 | 엘리먼트 바이오사이언스, 인크. | 핵산 시퀀싱을 위한 플로우 셀 디바이스 및 광학 시스템 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693489A (en) | 1984-03-30 | 1997-12-02 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
| US6872816B1 (en) * | 1996-01-24 | 2005-03-29 | Third Wave Technologies, Inc. | Nucleic acid detection kits |
| RU2143004C1 (ru) * | 1993-09-27 | 1999-12-20 | АРЧ Девелопмент Корпорейшн | Способ определения последовательности нуклеиновой кислоты (варианты) и набор для использования при определении последовательности нуклеиновой кислоты |
| JP4638043B2 (ja) * | 1998-12-14 | 2011-02-23 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法 |
| US6982146B1 (en) * | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
| US6529240B2 (en) * | 1999-11-18 | 2003-03-04 | Agilent Technologies, Inc. | Random access memory integrated with CMOS sensors |
| US6548264B1 (en) * | 2000-05-17 | 2003-04-15 | University Of Florida | Coated nanoparticles |
| EP1721000A4 (en) * | 2004-03-05 | 2008-08-20 | Univ New York State Res Found | USE OF RNA POLYMERASE AS MOLECULAR ENGINE DEPENDENT OF INFORMATION |
| US20070077575A1 (en) * | 2004-03-05 | 2007-04-05 | Mcallister William T | Use of rna polymerase as an information-dependent molecular motor |
| US7556922B2 (en) * | 2006-03-23 | 2009-07-07 | The Board Of Trustees Of The Leland Stanford Junior University | Motion resolved molecular sequencing |
| CA2707600C (en) * | 2007-12-04 | 2018-02-20 | Pacific Biosciences Of California, Inc. | Alternate labeling strategies for single molecule sequencing |
| US8815576B2 (en) * | 2007-12-27 | 2014-08-26 | Lawrence Livermore National Security, Llc. | Chip-based sequencing nucleic acids |
| KR101924665B1 (ko) | 2011-02-23 | 2018-12-03 | 이브 바이오메디컬, 인크. | 회전-의존성 전사 서열분석 시스템 및 사용 방법 |
-
2012
- 2012-02-23 KR KR1020137024640A patent/KR101924665B1/ko not_active Expired - Fee Related
- 2012-02-23 CN CN201280019968.8A patent/CN103534357B/zh not_active Expired - Fee Related
- 2012-02-23 CA CA2827880A patent/CA2827880A1/en not_active Abandoned
- 2012-02-23 AU AU2012220546A patent/AU2012220546B2/en not_active Ceased
- 2012-02-23 RU RU2013142965A patent/RU2636460C2/ru not_active IP Right Cessation
- 2012-02-23 JP JP2013555562A patent/JP6018092B2/ja not_active Expired - Fee Related
- 2012-02-23 US US13/403,645 patent/US8574840B2/en not_active Expired - Fee Related
- 2012-02-23 EP EP12709419.1A patent/EP2678444B1/en not_active Not-in-force
- 2012-02-23 WO PCT/US2012/026339 patent/WO2012116191A1/en not_active Ceased
-
2013
- 2013-11-04 US US14/070,904 patent/US9657343B2/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2013142965A (ru) | Системы и способы применения зависящего от вращения транскрипционного секвенирования | |
| JP2016507249A5 (enExample) | ||
| RU2015139609A (ru) | Способы и композиции для секвенирования нуклеиновой кислоты, основанные на наноструктуре | |
| CN107002006B (zh) | 用于检测目标基因的微流体装置、用于制造微流体装置的方法及用于使用微流体装置进行检测的方法 | |
| US9683230B2 (en) | Sample preparation on a solid support | |
| EP3259602B1 (en) | Method for rapid accurate dispensing, visualization and analysis of single cells | |
| JP2014513557A5 (enExample) | ||
| Qu et al. | Real-time continuous identification of greenhouse plant pathogens based on recyclable microfluidic bioassay system | |
| Sun et al. | Direct immobilization of DNA probes on non-modified plastics by UV irradiation and integration in microfluidic devices for rapid bioassay | |
| Sabourin et al. | Microfluidic DNA microarrays in PMMA chips: streamlined fabrication via simultaneous DNA immobilization and bonding activation by brief UV exposure | |
| JP7667739B2 (ja) | 固体支持体上で固定化されたｃｒｉｓｐｒ／ｃａｓ９を使用する核酸シークエンシングライブラリを調製するための組成物及び方法 | |
| Jensen et al. | Direct oligonucleotide synthesis onto super-paramagnetic beads | |
| US20210033606A1 (en) | DNA mapping and sequencing on linearized DNA molecules | |
| HK1187959B (en) | Rotation-dependent transcriptional sequencing systems and methods of using | |
| HK40096585A (zh) | 多路复用covid-19锁式测定 | |
| KIM et al. | An asymmetric PCR on film device and PNA hybridization for identifying fish species | |
| Cho | Ordered DNA Fragmentation and Amplification for Sequencing Applications | |
| CA3021915A1 (en) | Self-assembled patterning using patterned hydrophobic surfaces |